Preliminary results concerning the element content of Fusarium-infected wheat genotypes
12
Szeged – Timisoara axis for the safe food and feed SZETISA1 Hungary-Romania Cross-Border Co-operation Programme 2007-2013 HURO/0901/147/2.2.2. Preliminary results concerning the element content of Fusarium- infected wheat genotypes Timisoara, May 30-31, 2011 Andrea Pető Ph.D. student
description
Szeged – Timisoara axis for the safe food and feed SZETISA1 Hungary-Romania Cross-Border Co-operation Programme 2007-2013 HURO/0901/147/2.2.2 . Andrea Pető Ph.D . student. Preliminary results concerning the element content of Fusarium-infected wheat genotypes. - PowerPoint PPT Presentation
Transcript of Preliminary results concerning the element content of Fusarium-infected wheat genotypes
Szeged – Timisoara axis for the safe food and feedSZETISA1 Hungary-Romania Cross-Border Co-operation Programme 2007-2013HURO/0901/147/2.2.2.
Preliminary results concerning the element content of Fusarium-infected wheat genotypes
Timisoara, May 30-31, 2011
Andrea PetőPh.D. student
Materials and methods
Optima 7000 ICP-OES
AAS Hitachi Z-8200
MarsXPress CEM
• Sampling happened in summer of 2010• Measuring in autumn of 2010
•Department of Physical Geography and Geoinformatics, University of Szeged
Resistant lines
514
515504502
500
474
493415 518
484Sensitive linesWheat sampling
1. GK Mini Manó/Nobeokabozu population
Wheat sampling1. GK Mini Manó/Nobeokabozu population
A- grainB- chaffC-flag leafD-green earE-culm
Sensitive: 518 504 500 493 415
Resistant: 515 502 496 484 474
10 Elements were measured:Fe, Mn, Cu, Zn, Ni, Co, Cr, Cd, As, Se
Fe Mn Cu Zn Ni Co Cr Cd As Se -10.000
0.000
10.000
20.000
30.000
40.000
50.000
60.000
518 A Sensitive
Elements
μg/g
518 sample
Fe Mn Cu Zn Ni Co Cr Cd As Se -10.000
0.00010.00020.00030.00040.00050.00060.00070.00080.000
518 B Sensitive
Elements
μg/g
Fe Mn Cu Zn Ni Co Cr Cd As Se -20.000
0.00020.00040.00060.00080.000
100.000120.000
518 C Sensitive
Elements
μg/g
Fe Mn Cu Zn Ni Co Cr Cd As Se -10.000
0.000
10.000
20.000
30.000
40.000
50.000
60.000
70.000
518 D Sensitive
Elements
μg/g
Fe Mn Cu Zn Ni Co Cr Cd As Se -5.000
0.000
5.000
10.000
15.000
20.000
25.000
30.000
518 E Sensititve
Elements
μg/g
Fe Mn Cu Zn Ni Co Cr Cd As Se
-20.000
0.000
20.000
40.000
60.000
80.000
100.000
493 B Sensitive
Elements
μg/g
The lowest Mn values
Fe Mn Cu Zn Ni Co Cr Cd As Se
-10.000
0.000
10.000
20.000
30.000
40.000
50.000
60.000
500 B Sensitive
Elements
μg/g
Fe Mn Cu Zn Ni Co Cr Cd As Se
-20.000
0.000
20.000
40.000
60.000
80.000
100.000
484 B Resistant
Elements
μg/g
The highest Mn values
The highest Ca values The lowest Ca values493 B Sensitive -> 32,39 μmolCa/gDM 484 B Resistant -> 57,13 μmolCa/gDM
500 B Sensitive -> 17,3 μmolCa/gDM 496 B Resistant -> 48,86 μmolCa/gDM
B = chaff
Fe Mn Cu Zn Ni Co Cr Cd As Se -20.000
0.000
20.000
40.000
60.000
80.000
100.000
120.000
140.000
515 B Resistant
Elements
μg/g
grain green ear culm chaff flagleaf0
20
40
60
80
100
120
140
160
Ca content
SensitiveResistant
μmol
Ca/g
DM
Measured by AAS
grain green ear culm chaff flagleaf0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
Mn content
SensitiveResistant
μmol
Mn/
gDM
grain chaff green ear culm flagleaf05
101520253035404550
Znμg/g
grain chaff green ear culm flagleaf0
1
2
3
4
5
6
Cu
μg/g
grain chaff green ear culm flagleaf0
0.5
1
1.5
2
2.5
3
Se
μg/g
grain chaff green ear culm flagleaf0
20
40
60
80
100
120
Fe
ResistantSensitive
μg/g
Summarized data Measured by ICP
grain chaff green ear culm flagleaf0
10
20
30
40
50
60
70
80
90
Mnμg/g
Measured by AAS
grain green ear culm chaff flagleaf0.00
50.00
100.00
150.00
200.00
250.00
Ca contentμm
olCa
/gDM
grain green ear culm chaff flagleaf0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0.16
Cu content
μmol
Cu/g
DM
grain green ear culm chaff flagleaf0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
Mn content
Csillag InfectedKalász InfectedCsillag Not InfectedKalász Not Infected
μmol
Mn/
gDM
R S R S R S R S R Schaff grain green ear culm flagleaf
0
50
100
150
200
250
Element content
ZnCuSeMnFe
R - ResistantS - Sensitive
Measured by ICP
Our 0-hour-old samples - May, 2011
Culms, flagleaves and ears
474 – Resistant – high Mn content483 – Sensitive – high Mn content488 – Sensitive – low Mn content501 – Resistant – low Mn content
According to earlier results:
Thank you for your attention!
