PRELIMINARY PHYTOCHEMICAL STUDY OF FOUR PLANT …PowerPoint Presentation Author: 2 COOL Created...
Transcript of PRELIMINARY PHYTOCHEMICAL STUDY OF FOUR PLANT …PowerPoint Presentation Author: 2 COOL Created...
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SYAIFUDDIN1
ACEP AKBAR2
BUDI SUTIYA3
SITI HAMIDAH4
PRELIMINARY PHYTOCHEMICAL STUDY OF FOUR PLANT
SPECIES FROM MANGROVE AREA AT KURAU DISTRICT OF
TANAH LAUT SOUTH BORNEO PROVINCE
1,2 FORESTRY RESEARCH INSTITUTE BANJARBARU 3 ,4 LAMBUNG MANGKURAT UNIVERSITY BANJARBARU
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INTRODUCTION
1. One of the mangrove forest benefits is as source of medicinal
plants for human life
2. Species of mangrove forest in kurau as medicinal plants:
a. Api-api (Avicennia marina)
b. Bebutah (Excoecaria agallocha)
c. Jeruju (Acanthus ilicifolius)
d. Rambai (Sonneratia caseolaris)
3. Information about the active chemical compounds contained in
four mangrove species is not widely known
4. The Active Chemical Compounds: saponin, flavonoid, quinon,
steroid, triterpenoid, carotenoid, and alkaloid
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THE OBJECTIVE
The Objectives of this study is phytochemical testing to
identification of active chemical compounds contained in
api-api, bebutah, jeruju, and rambai
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A. Place:
1. Sample was taken in Mangrove forest Kurau
district of Tanah Laut, South Borneo
2. Phytochemical tests conducted in Laboratory of
FMIPA Lambung Mangkurat University Banjarbaru
METHODS
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B. Makes of simplicia • Sample: Leaf, Wood, Bark, and Root
• Sample was dried (20°-25°C) and then mashed into powder
C. Phytochemical analysis: Make the test solution for identification saponin, flavonoid, and quinon:
1 gr simplicia included in 100 ml of boiling water for 5 minutes
• Saponin: 10 ml the test solution shaken for 10 seconds, then left for 10
minutes, foam found in the test solution
• Flavonoid: 5 ml the test solution, 5 mg Mg, 1 ml concentrated HCl, half of
the ethanol solution, red-orange
• Quinon: 3 drops of 1 N NaOH solution into 5 ml of test solution, red
Make the filtrate for identification steroid and triterpenoid:
1 gr simplicia, 2 ml methanol, shaken, filtered, the filtrate was taken
• Steroid and triterpenoid: the filtrate, 2 drops C2H4O2, 2 drops H2SO4
Steroid: the test solution to be bluish green
Triterpenoid: the test solution to be purplish red
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• Karotenoid: 2 gr simplicia, 5 ml CHCl3, 5 ml C2H5OH, heated for 5 minutes, added water, shaken, red-orange
• Alkaloid: 2 gr simplicia, 5 ml CHCl3, 5 ml NH3, heated for 5 minutes, shaken, filtered, 5 ml H2SO4, shaken, taken the top layer, divided into 3 parts
mayer reagent white sediment,
wagner reagent the test solution to be brown,
dragendorf reagent the test solution to be red
(Source: FMIPA Lambung Mangkurat University Banjarbaru)
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1. Api-api (Avicennia marina Forsk)
Potentially
Treating
sunburn
RESULTS
(a) (b) (c) (d)
Part of
Plant
The Active Chemical Compound
Saponin Flavonoid Quinon Steroid Triterpenoid Carotenoid Alkaloid
Leaf - - - + - + +
Wood + - - - - - -
Bark - - - + - - -
Root + - - - - - -
a. Leaf b. Wood c. Bark d. Root
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2. Bebutah (Excoecaria agallocha Linn)
Part of
Plant
The Active Chemical Compound
Saponin Flavonoid Quinon Steroid Triterpenoid Carotenoid Alkaloid
Leaf - - - + - + -
Wood + - - - - - -
Bark - + + - + - +
Root - - + - + - +
(a) (b) (c) (d)
Potentially
Cure the
swollen
a. Leaf b. Wood c. Bark d. Root
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Part of
Plant
The Active Chemical Compound
Saponin Flavonoid Quinon Steroid Triterpenoid Carotenoid Alkaloid
Leaf - - - + - + +
Wood - - - + - - -
Bark - - - + - - -
Root - - - + - - -
(a) (b) (c) (d)
3. Jeruju (Acanthus ilicifolius Linn)
Potentially
Sores
recovery
a. Leaf b. Wood c. Bark d. Root
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Part of
Plant
The Active Chemical Compound
Saponin Flavonoid Quinon Steroid Triterpenoid Carotenoid Alkaloid
Leaf - + + + - + +
Wood + - - - - - -
Bark + + + - + - -
Root - - + + - - -
(a) (b) (c) (d)
4. Rambai (Sonneratia caseolaris Linn)
Potentially
Cold powder
to make the
skin smooth,
tighten the
skin, removes
spots and
pimples on the
skin.
a. Leaf b. Wood c. Bark d. Root
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CONCLUSION
Four species of mangrove forest at Kurau Subdistrict
area, in Tanah Laut South Borneo Province namely api-
api, bebutah, jeruju, and rambai contain saponins,
flavonoids, quinone, steroids, triterpenoids, carotenoids,
and alkaloids
Pharmacology, toxicity, and clinical test of four
mangrove species mentioned are strongly recommended
for safely used of medicine by society