C297-E055BPPSQ-31A

/ 33A

Protein Sequencer

PPSQ-31A / 33A

Printed in Japan 3655-05318-30AMF

Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.

For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

© Shimadzu Corporation, 2013www.shimadzu.com/an/

Achieving Greater Simplicity and Reliabil ity in the Determination of Amino Acid Sequences

Repeated Use of Mobile PhaseReduced running costs and l iquid waste in theanalysis of PTH-amino acids using isocratic mode

Software with Excellent Operabil itySimple operations andeasy-to-use data analysis functions

Analytical Stabil ityExcellent baseline stabil ity andretention time reproducibil ity

Protein Sequencer

PPSQ-31A / 33AP. 4

P. 5

P. 5

Achieving Greater Simplicity and Reliabil ity in the Determination of Amino Acid Sequences

Repeated Use of Mobile PhaseReduced running costs and l iquid waste in theanalysis of PTH-amino acids using isocratic mode

Software with Excellent Operabil itySimple operations andeasy-to-use data analysis functions

Analytical Stabil ityExcellent baseline stabil ity andretention time reproducibil ity

Protein Sequencer

PPSQ-31A / 33AP. 4

P. 5

P. 5

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25

0.00

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

mV

D

E

TH

N Q S

G AY R

M VP W

F

K

I L

PTH-AA

2D Electrophoresis Image of Proteins Extracted from E. Coli

10pmol

Spot 1

Sequence analysis can be started by simply registering

the number of analysis cycles, data file names, and other

information, and then clicking the [Start] button.

When a reagent is replaced, the inside of the reagent bottle is

purged with nitrogen. The replacement dates and times of the

reagents can be ascertained with a glance, and lot control is

possible.

Analytical Stabil ity

Baseline Stabil ity

Repeated Use of Mobile Phase

PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.

Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.

Software with Excellent Operabil ity

Simple OperationsSpecialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.

Main Window

Sequence Schedule Window Reagent Control Window

Retention Time Reproducibil ity

Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)

Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.

cycle 1 cycle 2

cycle 14 cycle 15

cycle 24 cycle 25

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

DTT

S

DMPTU DPTU

DPU DTT

T

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

DMPTU

LDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

Y

DTT DPTU

DMPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

EDTT

DMPTUDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50mV

K

DTT

DMPTUDPTU

Selection of sequencing schedule

Summary display of currently executed cycle and step and estimated completion time

4 5PPSQ-31A / 33A

Protein Sequencer

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 min-0.25

0.00

0.25

0.50

0.75

1.00

1.25

1.50

1.75

2.00

mV

D

E

TH

N Q S

G AY R

M VP W

F

K

I L

PTH-AA

2D Electrophoresis Image of Proteins Extracted from E. Coli

10pmol

Spot 1

Sequence analysis can be started by simply registering

the number of analysis cycles, data file names, and other

information, and then clicking the [Start] button.

When a reagent is replaced, the inside of the reagent bottle is

purged with nitrogen. The replacement dates and times of the

reagents can be ascertained with a glance, and lot control is

possible.

Analytical Stabil ity

Baseline Stabil ity

Repeated Use of Mobile Phase

PPSQ series protein sequencers separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids.

Analysis of PTH-Amino Acids Using Isocratic ModePerforming PTH-amino acid analysis in an isocratic mode in which eluents are recycled to allow repeated use of the mobile phase makes it possible to reduce liquid waste and running costs.

Software with Excellent Operabil ity

Simple OperationsSpecialized protein sequencer software incorporating control functions for the reaction unit and HPLC analysis unit makes it easy to perform sequence analysis.

Main Window

Sequence Schedule Window Reagent Control Window

Retention Time Reproducibil ity

Analysis Examples for Spot 1 (The results for cycle 2 onwards are subtraction chromatograms.)

Isocratic sequence analysis provides more stable retention times. Therefore, peaks detected in previous cycles can be cancelled using substation chromatogram processing, making it easier to identify sequences.

cycle 1 cycle 2

cycle 14 cycle 15

cycle 24 cycle 25

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

DTT

S

DMPTU DPTU

DPU DTT

T

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.0

0.2

0.4

0.6

0.8

1.0 mV

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

DMPTU

LDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

Y

DTT DPTU

DMPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50 mV

EDTT

DMPTUDPTU

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

0.00

0.10

0.20

0.30

0.40

0.50mV

K

DTT

DMPTUDPTU

Selection of sequencing schedule

Summary display of currently executed cycle and step and estimated completion time

4 5PPSQ-31A / 33A

Protein Sequencer

Reprocessing of chromatograms required

for data analysisOverlay of multiple

chromatograms

Automatic Sequence Estimation

Automatic estimation of amino acid sequences

Chromatogram peak integration, printing, and other

processes can be performed on a per-sample basis.

Displays multiple chromatograms. Analysis cycles can be

updated simply by clicking a button.

Window allows easy identification of sequences.

Calibrates PTH-amino acids.

Retention times can be edited simply by selecting

a PTH-amino acid and clicking a peak.

Automatically generates a report containing analytical parameters,

estimated sequences, yield rates, and other information.

If the appropriate settings are entered before analysis

starts, sequences are automatically estimated after each

cycle is complete. Up to four candidate amino acids are

displayed together with their certainty levels.

Customized Reports

Reports containing information such as analytical

parameters, estimated sequences, and yield rates are

automatically created.

Yield Graph Display

Initial and repeated yield rates are calculated and

displayed in graph form. Amino acids used for

calculations can be freely selected.

Reaction method

Reaction time

Number of reactors

Sample immobilization method

Reactor temperature control

Converter temperature control

Column temperature control

Number of reagents/solvents

Reagent/solvent delivery method

Dimensions

Weight

Edman degradation

PPSQ-31A: 47 min/cycle

PPSQ-33A: 48.5 min/cycle

PPSQ-31A: 1

PPSQ-33A: 3

Glass fiber disk (8 mm dia.) or PVDF membrane

Room temperature + 10 °C to 60 °C

Room temperature + 10 °C to 70 °C

Room temperature + 10 °C to 60 °C

8 (one is an option)

Nitrogen gas pressure

W650 × D500 × H520 mm

PPSQ-31A: 55 kg

Main Unit

Reagents for amino acid sequence analysis (7 types)

Glass fiber disks

Polybrene reagent

Apomyoglobin standard

Mobile phase for PTH-amino acids

Separation column for PTH-amino acids

Mixed standards for PTH-amino acids

1 of each type

50

1

1

1 L

1

2 mL x 4

Part name QuantityPPSQ-31A/33A

Installation space

Power supply

Nitrogen gas

Exhaust equipment

Desktop: W1,730 × D600 × H550 mm min.

(Weight: Approx. 93 kg)

100 VAC, 1,100 VA, 50/60 Hz

Purity: 99.9999% min.

A cylinder pressure regulator and gas tubing

(10 m) are provided as standard accessories.

In order to provide an exhaust for gases

produced by waste liquids, an exhaust tube

must either be connected to exhaust

equipment or led outside. An exhaust tube

(20 m) is provided as a standard accessory.

Flow rate setting range

Dimensions

Weight

0.001 to 10.000 mL/min

W260 × D420 × H140 mm

11 kg

Solvent Delivery Module LC-20AT

292-31310-XX

292-05684-92

PPSQ-31A (main unit)

LC-20AT

SPD-20A

PPSQ-30 (control PC set)

Startup kit

Part number Part name

Wavelength setting range

Dimensions

Weight

190 to 700 nm

W260 × D420 × H140 mm

13 kg

Detector SPD-20A

Computer

Display

Note: In the interests of product improvement, these specifications may change without notice.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.

Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.

Note: The required installation space and power supply may change according to the PC and display used.

Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.

Offers highly cost-effective performance.

PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)

IBM PC/AT or compatible

OS: Windows® XP, Windows 7

17-inch LCD

Control PC

Standard Configurations

Startup Kit

Other Items Provided by User

PPSQ-31A System

292-31330-XX

292-05684-92

PPSQ-33A (main unit)

LC-20AT

SPD-20A

PPSQ-30 (control PC set)

Startup kit

Part number Part name

PPSQ-33A System

PPSQ-31A Single Reactor

Specifications

Simple, Easy-to-Use Data Analysis FunctionsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.

6 7PPSQ-31A / 33A

Protein Sequencer

Reprocessing of chromatograms required

for data analysisOverlay of multiple

chromatograms

Automatic Sequence Estimation

Automatic estimation of amino acid sequences

Chromatogram peak integration, printing, and other

processes can be performed on a per-sample basis.

Displays multiple chromatograms. Analysis cycles can be

updated simply by clicking a button.

Window allows easy identification of sequences.

Calibrates PTH-amino acids.

Retention times can be edited simply by selecting

a PTH-amino acid and clicking a peak.

Automatically generates a report containing analytical parameters,

estimated sequences, yield rates, and other information.

If the appropriate settings are entered before analysis

starts, sequences are automatically estimated after each

cycle is complete. Up to four candidate amino acids are

displayed together with their certainty levels.

Customized Reports

Reports containing information such as analytical

parameters, estimated sequences, and yield rates are

automatically created.

Yield Graph Display

Initial and repeated yield rates are calculated and

displayed in graph form. Amino acids used for

calculations can be freely selected.

Reaction method

Reaction time

Number of reactors

Sample immobilization method

Reactor temperature control

Converter temperature control

Column temperature control

Number of reagents/solvents

Reagent/solvent delivery method

Dimensions

Weight

Edman degradation

PPSQ-31A: 47 min/cycle

PPSQ-33A: 48.5 min/cycle

PPSQ-31A: 1

PPSQ-33A: 3

Glass fiber disk (8 mm dia.) or PVDF membrane

Room temperature + 10 °C to 60 °C

Room temperature + 10 °C to 70 °C

Room temperature + 10 °C to 60 °C

8 (one is an option)

Nitrogen gas pressure

W650 × D500 × H520 mm

PPSQ-31A: 55 kg

Main Unit

Reagents for amino acid sequence analysis (7 types)

Glass fiber disks

Polybrene reagent

Apomyoglobin standard

Mobile phase for PTH-amino acids

Separation column for PTH-amino acids

Mixed standards for PTH-amino acids

1 of each type

50

1

1

1 L

1

2 mL x 4

Part name QuantityPPSQ-31A/33A

Installation space

Power supply

Nitrogen gas

Exhaust equipment

Desktop: W1,730 × D600 × H550 mm min.

(Weight: Approx. 93 kg)

100 VAC, 1,100 VA, 50/60 Hz

Purity: 99.9999% min.

A cylinder pressure regulator and gas tubing

(10 m) are provided as standard accessories.

In order to provide an exhaust for gases

produced by waste liquids, an exhaust tube

must either be connected to exhaust

equipment or led outside. An exhaust tube

(20 m) is provided as a standard accessory.

Flow rate setting range

Dimensions

Weight

0.001 to 10.000 mL/min

W260 × D420 × H140 mm

11 kg

Solvent Delivery Module LC-20AT

292-31310-XX

292-05684-92

PPSQ-31A (main unit)

LC-20AT

SPD-20A

PPSQ-30 (control PC set)

Startup kit

Part number Part name

Wavelength setting range

Dimensions

Weight

190 to 700 nm

W260 × D420 × H140 mm

13 kg

Detector SPD-20A

Computer

Display

Note: In the interests of product improvement, these specifications may change without notice.Note: Windows is a registered trademark of Microsoft Corporation in the U.S. and other countries.

Note: The startup kit is for initial installation. To order additional reagents, contact Wako Pure Chemical Industries, Ltd.

Note: The required installation space and power supply may change according to the PC and display used.

Using a triple reactor makes it possible to save time and effort, and gives greater freedom in the formulation of analysis programs.

Offers highly cost-effective performance.

PPSQ-33A Triple Reactor (Supports consecutive analysis of 3 samples.)

IBM PC/AT or compatible

OS: Windows® XP, Windows 7

17-inch LCD

Control PC

Standard Configurations

Startup Kit

Other Items Provided by User

PPSQ-31A System

292-31330-XX

292-05684-92

PPSQ-33A (main unit)

LC-20AT

SPD-20A

PPSQ-30 (control PC set)

Startup kit

Part number Part name

PPSQ-33A System

PPSQ-31A Single Reactor

Specifications

Simple, Easy-to-Use Data Analysis FunctionsSpecialized protein sequencer software makes it simple to perform the reprocessing of chromatograms, the overlay of multiple chromatograms, and the automatic estimation of amino acid sequences, which are required for sequence analysis.

6 7PPSQ-31A / 33A

Protein Sequencer

C297-E055BPPSQ-31A

/ 33A

Protein Sequencer

PPSQ-31A / 33A

Printed in Japan 3655-05318-30AMF

Company names, product/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services. Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.

For Research Use Only. Not for use in diagnostic procedures. The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

© Shimadzu Corporation, 2013www.shimadzu.com/an/