PowerPoint Presentation · The large subunit is composed of a 5S RNA (120 nucleotides), 28S RNA...
Transcript of PowerPoint Presentation · The large subunit is composed of a 5S RNA (120 nucleotides), 28S RNA...
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ribosome
• Bacterial 30S (small) subunit
• 16S rRNA (orange)
• 21 proteins (blue)
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ribosome
• Bacterial 50S (small) subunit
• 26S rRNA (orange)
• proteins (blue)
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(from Wikipedia)
• Eukaryotes have 80S ribosomes, each consisting of a small (40S) and large (60S) subunit. Their 40S subunit has an 18S RNA (1900 nucleotides) and 33 proteins. The large subunit is composed of a 5S RNA (120 nucleotides), 28S RNA (4700 nucleotides), a 5.8S RNA (160 nucleotides) subunits and 46 proteins.
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• blast your SPAdes assembly against known rDNA sequence
• Use grep –A 1 to pull out that sequence from the assembly
• Download to your computer
• Blast against NCBI to see what it hits, confirm it is what we want
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• blast your SPAdes assembly against known rDNA sequence
• Use grep –A 1 to pull out that sequence from the assembly
• blast your SPAdes assembly against known mitochondrial sequence
• Use grep –A 1 to pull out that sequence from the assembly
• Download each sequence to your computer
• Blast against NCBI to see what it hits, confirm it is what we want