PowerPoint Presentation€¦ · PPT file · Web view · 2014-06-19Insulin Bioreactor Design...
Transcript of PowerPoint Presentation€¦ · PPT file · Web view · 2014-06-19Insulin Bioreactor Design...
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Presented by:Shehneela Baseer
117113Zainab Sajjad
117114
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Introduction to Bioreactors Types of Bioreactor designs Conclusion
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Any manufactured or engineered device or system that supports a biologically active environment
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Stirred tank reactors Bubble-column reactors Air lift reactors Drum rotating reactors Immobilized plane cell reactors
Membrane reactors
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Air is dispersed by mechanical agitation.
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Better control over the environment of the culture.
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Can cause damage to the cells
High energy demand
Complexity in construction
Difficult to scale up.
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One of the simplest type of gas – liquid bioreactors.
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Facilitates sterile operation
Less damaging to shear-sensitive cells
Scale up is relatively easy.
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Undefined fluid flow pattern inside the reactor.
Non-uniform mixing.
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Works on draught tube principle.
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Reasonable mixing with low shear
Operating cost is low.
Less contamination
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Insufficient mixing at high cell densities.
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Consists of horizontally rotating-drum on rollers connected to a motor.
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High oxygen transfer.
Good mixing
Facilitated better growth and impart less hydrodynamic stress.
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Difficult to scale up.
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Immobilization of plant cell into a suitable carriers.
Either in natural (alginate, agar) or synthetic (polyacrylamide)
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Cells are separated from growth medium by membrane
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Environment is more easily controlled
Better control over cell density.
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Reactor type
Oxygen transfer
Hydrodynamic stress
Mixing Scale up limitations
Stirred-tank High Highly destructive
Completely uniform
Difficult Cell death; contamination due to moving parts
ST-low agitation and modified impeller
Medium Low Reasonably uniform
Difficult Insufficient mixing at very high cell densities
Bubble-column
Medium Low Non-uniform
Easy Dead zones; settling of cells due to poor mixing
Air-lift High Low Uniform Easy Dead zones at high cell densities
Rotating-drum
High Low Uniform Difficult Non-uniform mixing at very large scale.
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Insulin Bioreactor Design
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Production of insulin precursor 5000 kg insulin per year
Assumes 20 % loss due to purification kLa within 10% of 2088 hr -1
Prevents oxygen limited reactionPrevents anaerobic metabolism
Glucose concentration < 0.5 g/LPrevents formation of ethanol
CSTR configuration Jacket heat exchanger Price
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Substrate feedGlucose, ammonia, mineral salts
Cellular metabolism of substrate Extracellular production of insulin Air sparging for oxygen delivery Impellers for mixing of nutrients and
oxygen
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Hence, with the help of different types of bioreactors, commercial production of secondary metabolites is not only possible but also profitable.
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