Power-point presentation template · sample prep. drop on grid / grid on drop . CODA-CERVA on-grid...
Transcript of Power-point presentation template · sample prep. drop on grid / grid on drop . CODA-CERVA on-grid...
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AutoEM-toolbox
NanoDefine Outreach September 20th 2017
Brussels Toni Uusimäki, Ralf Kaegi
Eawag
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Typical workflow
Sample prep. Image acquisition Image analysis
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Image acquisition
Toni Uusimäki, Ralf Kaegi
Achievements of NanoDefine
Image analysis Thorsten Wagner
sample prep.
drop on grid / grid on drop
CODA-CERVA
on-grid centrifugation
USN e-depo. Electrospray
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AutoEM-toolbox TEM mode
Main Tab Calibrations
User Interface
Camera parameters
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AutoEM-toolbox TEM mode
UI
Live PSD Live view
ParticleSizer results
3D Segmenation 3D recorded images
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AutoEM-toolbox TEM mode
TEM bright field image of Au/SiO2 NPs Pixel size: 2.95 nm FOV: 3 µm
A mosaic collection of TEM images (35x10, 1.4 gb) with FOV 0.11 mm x 0.3 mm
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AutoEM-toolbox STEM mode
STEM HAADF image of SiO2 NPs (ERM-FD304) Pixel size: 1.17 nm FOV: 1.2 µm
A mosaic collection of STEM images (10x5, 0.2 gb) with FOV 12 µm x 6 µm
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AutoEM-toolbox STEM EDS mode
- User chooses the elements and defines Cutoff values for the peaks - The EDS spectra integrated from the SI image is smoothed and an optimized Gaussian curve is fitted to the peak - If the peak is above the cutoff value, the feret min value will be added to the PSD
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AutoEM-toolbox STEM EDS mode
Au/Si NPs (5x4) FOV 5 µm x 4 µm
PSD of Au NPs
PSD of Si NPs
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AutoEM-toolbox TEM EFTEM mode Thickness Map
ZLP image Unfiltered image Thickness Map t/λ
𝑡𝑡λ = ln
𝐼𝐼𝐼𝐼0
, λ = 106𝐹𝐹 𝐸𝐸0
𝐸𝐸𝑀𝑀�
𝑙𝑙𝑙𝑙 2𝐸𝐸0𝛽𝛽𝐸𝐸𝑀𝑀�
=155.5 nm,
Ref: Egerton & Chang, Ultramicroscopy 21 (1987) 231-244
𝐹𝐹 = 1+𝐸𝐸0 1022�
1+ 𝐸𝐸0511�
2, 𝐸𝐸𝑀𝑀 = 7.6𝑍𝑍𝑒𝑒𝑒𝑒𝑒𝑒0.36
λ = 𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚 𝑓𝑓𝑓𝑓𝑚𝑚𝑚𝑚 𝑝𝑝𝑚𝑚𝑡𝑡𝑝, 𝑡𝑡 = 𝑡𝑡𝑝𝑡𝑡𝑡𝑡𝑡𝑡𝑚𝑚𝑚𝑚𝑡𝑡𝑡𝑡, 𝐼𝐼 = 𝑢𝑢𝑚𝑚𝑓𝑓𝑡𝑡𝑢𝑢𝑡𝑡𝑚𝑚𝑓𝑓𝑚𝑚𝑢𝑢 𝑡𝑡𝑚𝑚𝑚𝑚𝑖𝑖𝑚𝑚, 𝐼𝐼0= 𝑍𝑍𝑍𝑍𝑍𝑍 𝑡𝑡𝑚𝑚𝑚𝑚𝑖𝑖𝑚𝑚 𝐸𝐸0 = 𝑡𝑡𝑚𝑚𝑡𝑡𝑡𝑡𝑢𝑢𝑚𝑚𝑚𝑚𝑡𝑡 𝑚𝑚𝑚𝑚𝑚𝑚𝑓𝑓𝑖𝑖𝑒𝑒 𝑜𝑜𝑓𝑓 𝑚𝑚𝑢𝑢𝑚𝑚𝑡𝑡𝑡𝑡𝑓𝑓𝑜𝑜𝑚𝑚𝑡𝑡
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AutoEM-toolbox TEM: Thickness maps
Illite NPs 3D image extracted from the Thickness Map
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AutoEM-toolbox TEM EFTEM mode
Illite NPs (10x12, 2 gb) FOV 47 µm x 57 µm
PSD XY analysis
PSD 3D analysis
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AutoEM-toolbox
STEM TEM
EELS
EDS Core Loss Imaging
Thickness Map
Particle Sizer
Custom Tasks
Elemental composition
Elemental composition
Elemental composition
Thickness (Z)
Size (XY)
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x50 = 180 nm
AutoEM-toolbox: A little treaser
BaSO4 – TiO2
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AutoEM-toolbox
STEM TEM
EELS
EDS Core Loss Imaging
Thickness Map
Particle Sizer
Custom Tasks
Elemental composition
Elemental composition
Elemental composition
Thickness (Z)
Size (XY)
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www.NanoDefine.eu
This project has received funding from the European Union’s Seventh Programme for research, technological development and demonstration under grant agreement No 604347