Potentialof Different Poplar Clones in Phytoremediation of ...
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Potential of Different Poplar Clones in Phytoremediation of Some Heavy Metals Andrej Pilipovic 1 , Sasa Orlovic 1 , Natasa Nikolic 2 , Zoran Galic 1 1 Institute of Lowland Forestry and Environment, Novi Sad, Serbia 2 Faculty of Science - Institute of Biology and Ecology, Novi Sad, Serbia
Transcript of Potentialof Different Poplar Clones in Phytoremediation of ...
Potential of Different Poplar Clones in Phytoremediation of Some Heavy Metals
Andrej Pilipovic1, Sasa Orlovic1, Natasa Nikolic2, ZoranGalic1
1 Institute of Lowland Forestry and Environment, Novi Sad, Serbia2 Faculty of Science - Institute of Biology and Ecology, Novi Sad, Serbia
Comtaminants
Nickel• Microlelement
• Necrosis
• Fe defficiency
Lead• Sistemic toxine
• binding with metaloenzymes
• inhibiting synthesis of proteins
• inhibiting synthesis of chloroplast andphotosynthesis
Cadmium•Chlorosis
•Disorder of respiration and nitrogen metabolism
•Growth inhibition
Previous research
Pilipović, A., Nikolić, N., Orlović, S., Petrović, N., Krstić, B. (2005) Cadmium phytoextraction potential of polar clones (Populus spp.). Z. Naturforsch. 60c:
247-251.
Nikolić, N.; Pilipović, A.; Orlović, S.; Krstić, B.; Pajević, S.(2005) The Potential of Poplars in Cleaninig Waters Contaminated by Cadmium.International Symposium on Danube Basin and Sustainable Development:
Environment, Tourism, Legal Framework. Novi Sad, Serbia & Montenegro, 28-29
September 2005.
Nikolić, N.; Pajević, S.; Pilipović, A.; Krstić, B.; Borišev, M.; (2006) The Potential of Poplars (Populus sp.) in remediation of lead contaminated environment. II International Szmposium of Ecologists of Montenegro, Kotor,
Montenegro, 20-24.09.2006.
Material & methods
� Hydroponically grown cuttings of:� Populus deltoides clone 37
� Populus x euramericana clone 48
� (P. nigra x maximowitzii) x P. nigravar. “Italica” clone 1044,
� 0, 10 or 100 ppm Ni, Cd or Pb
� 135 days ( 45+90)
Material & methods
� Investigated parameters� Biomass production� Nitrate reductase activity (NRA)� Accumulation of heavy metals in roots, leaves and stem
� Bioconcentration factor (Phytoextraction coefficient) (Dickinson & Puloford, 2005)
BCF = C(plant)/C(medium)
� BCF = 0-1����� BCF > 1
Biomass production M (gDW)
a
abbc
a
a
c
a a
bc
0
10
20
30
40
cl. 1044 cl. 37 cl. 48
0 10 100
a
d
d
b b
cd
bc
d
d
0
10
20
30
40
cl.1044 cl. 37 cl. 48ab
cd d
a
cd
abc
bcd
cdcd
0
5
10
15
20
25
cl. 1044 cl. 37 cl. 48
Nickel
Cadmium
Lead
NRA in leaves (µmol NO2g-1
DWh-1)
b
dee
a
bccde bcd bc
e
0
0,5
1
1,5
2
2,5
3
cl. 1044 cl. 37 cl. 48
0 10 100
b
e
f
b
b
cd
c
f f
0
1
2
3
4
cl.1044 cl. 37 cl. 48a
c
d
a
d d
bc
ab
d
0
0,5
1
1,5
2
2,5
3
3,5
cl. 1044 cl. 37 cl. 48
Nickel
Cadmium
Lead
NRA in Roots (µmol NO2g-1
DWh-1)
bc
cdbcd
a
cd
bc
b
cd d
0
0,1
0,2
0,3
0,4
0,5
cl. 1044 cl. 37 cl. 48
0 10 100
b
cd
a
f
de e
fde
bc
0
0,1
0,2
0,3
0,4
0,5
0,6
cl. 1044 cl. 37 cl. 48
Nickel
Cadmium
Lead
d
c
a
d
e
b
d
e
d
0
0.1
0.2
0.3
0.4
0.5
cl. 1044 cl. 37 cl. 48
BCF (stem)
0,97
7,29
20,29
2,18
13,83
21,53
0,64 4,36
24,54
0
10
20
30
Pb Ni Cd Pb Ni Cd Pb Ni Cd
Clone No 1044. Clone No 37. Clone No 48.
BCF (leaf)
0,57
38,05
7,29
0,36
31,22
4,52
0,54
38,53
16,58
0
10
20
30
40
Pb Ni Cd Pb Ni Cd Pb Ni Cd
No 1044. No 37. No 48.
BCF (roots)
1320,39
177,25
370,38
773,82
106,53
265,85
1557,35
138,85
291,80
0
400
800
1200
1600
Pb Ni Cd Pb Ni Cd Pb Ni Cd
No 1044. No 37. No 48.
Conclusions
� Biomass production was affected by presence of heavy metals
� NRA was affected at highest concentrations
� Cd phytoextraction → stem
� Ni phytoextraction → leaves
� Pb phytoextraction → roots
� Stem of clone 37 is suitable for phytoextraction of Ni and Pb
� Leaves of clone 48 have high BCF of Cd
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