Potential applications for Oxford Nanopore sequnecing
-
Upload
yaniv-erlich -
Category
Science
-
view
419 -
download
0
Transcript of Potential applications for Oxford Nanopore sequnecing
![Page 1: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/1.jpg)
Problem: We Don’t Know What Fish We’re Eating
The problem is occurring at a variety of venues, though sushi restaurants are the worst
The mislabeling is happening for most fish and the true product is unpredictable
The problem is not just in NYC
Fish in the US is being mislabeled in alarming proportions
SEE FOOD: Genetic Verification for Proper Seafood Labeling Jacob Best
![Page 2: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/2.jpg)
Solution: See Food See Food is a start up that genetically certifies restaurants that are correctly labeling fish
TM
What? Why? How? Where?● Our company
provides certification to restaurants that have correctly labeled their fish products
● Certifications will be available online and displayed publically so consumers know it’s safe to eat there!
● Our goal is to provide transparency for fish consumption at the level closest to the consumer
● We want people to know what they are eating
● See Food uses MinIon sequencing and alignment to the NR database
● To make this cheap and effective See Food will collect only a handful of samples from a type of fish and at times unknown to the restaurant owners
● While mislabeling is a problem at types of venues, the biggest violator is sushi restaurants
● See Food’s verification can be a big leg up in the sushi restaurant industry and this is where we plan to start
![Page 3: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/3.jpg)
Feasibility and Future
Random Samplings
• See food will only collect a handful of samples (say 5-8 a month)
• But it will do so on random days at random times and to random fish so as to avoid fraud
Cost Effective
• Because there are only a limited number of samples it will not be too expensive
• The variable costs are roughly $2,500 to $4,000 per restaurant
Competitive Advantage
• In an industry plagued by uncertainty, genetic verification of fish labeling will provide businesses the opportunity to capture market share very quickly
Growth Into New Venues
Growth Into New Cities
Sushi restaurants Other restaurants and super markets
![Page 4: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/4.jpg)
Mobile Microbiome Sequencing
Benjamin Brittain
![Page 5: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/5.jpg)
Why analyze your microbiome?
![Page 6: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/6.jpg)
Why mobile sequencing?
![Page 7: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/7.jpg)
GenomcademyAdam Gayoso
ajg2188
![Page 8: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/8.jpg)
Codecademy for Genomics
• Empower those wanting to join the ubiquitous genomics movement with interactive lessons in biology and programming (citizen science)
• Inspire people to discover new applications of mobile sequencing, which will only become more popular and cheaper in the next 5-10 years, by giving them the knowledge necessary to interact with genomic data
• General knowledge lessons on impact genomics has and can have on human health, as well as understanding ethical implications
• Targeted towards quantitatively inclined individuals, as well as those wanting a higher level overview of how genomics impacts their lives
• Monetize via advertising or freemium model
![Page 9: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/9.jpg)
Challenges
• Only one mass-produced mobile sequencer
• Expensive cost of flow cell makes citizen science a bit harder
• Applications of knowledge limited to this one sequencer (MinION)
• Value to average person isn’t currently very high
• Finding balance between interactivity and technicality given the many prerequisites necessary for interpreting genomic data
Goals• Bring genomics education to masses, enabling better citizen science and potential for
applications we haven’t thought of• Become centralized knowledge resource for a wide-ranging field
![Page 10: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/10.jpg)
Metagenomic Sequencing for Acute Febrile Illnesses Kimberly Hou
![Page 11: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/11.jpg)
Key Features
Online platform that analyzes nanopore sequenced data from patients’ saliva samples and makes preliminary diagnoses + treatment options for the patient
Knowledge base of treatment options crowd-sourced from certified parties such as nurses and physicians
Mobile sequencing advantageous over Illumina and other technology with <6hr sample-to-answer turnaround time and longer read lengths
Tracks metadata such as location of patient, patient ID, etc. as part of data processing to look out for trends
![Page 12: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/12.jpg)
Considerations
Advantages❏ Big Picture❏ Comprehensiv
e❏ Universal
Challenges❏ HIPAA
Compliance❏ Data Storage❏ minION Sales
![Page 13: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/13.jpg)
MOBILE SEQUENCER FOR MISSING PERSONS
IDENTIFICATION SIKAI HUANG
Help me back home!!!
![Page 14: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/14.jpg)
BACKGROUND• 797,500 children reported missing in a one-year.• About 64.34% of missing children can be found.• Difficulties in bringing the missing children back home
• Advantages of MOBILE SEQUENCER:
1. Some children were kidnapped at a really young age. 2. Some are disabled 3. International child-trafficking …..
1. Portable and efficient2. Solve the difficulties above
![Page 15: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/15.jpg)
• Process:
• Doubt:
DNA info
Basic info
Basic Info
DNA info
1. Profitability?? It is a VC pitch !!! 2. Accuracy ??
![Page 16: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/16.jpg)
Nascent:Making Mothers Aware
Stephanie Huang
![Page 17: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/17.jpg)
Changing the prenatal experience once mother at a time
We provide the kit and software needed for OB/GYNs to help expecting mothers do on-the-spot genetic screenings and analysis.
High-risk pregnancy rates are on the rise.
Prenatal screening is time-sensitive: current tests require ~1 week for results. Amniocentesis results take 2-3 weeks.
![Page 18: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/18.jpg)
Challenges & Potential
Challenges:● Error rate: false positives and false negatives are both dangerous.● Sequencing mixed samples -- but we saw that it’s possible differentiate a mixed sample.● Training doctors/nurses to use the device and software -- but we were able to learn how
to use the MinION quickly.● Developing a primer mix and easy sample prep protocol.
Potential:● Expand to on-site doctor’s screenings for general patients.
![Page 19: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/19.jpg)
Mobile Sequencer for Food Safety Inspection
Wenrui HuangDec. 9th, 2016
![Page 20: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/20.jpg)
Have you ever wondering….
Meanwhile, 40% of food were trashed by grocery stores each year, which is equivalent to 165 billion dollars*!!! Huge waste!!!*data according to USDA reports
Half empty milk just expired
Leftover food kept in fridge for a few days
A partially rotten apple
Use them or throw away???
Are they still safe to eat???
Mobile Sequencer can provide the answer!!
![Page 21: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/21.jpg)
UNSAFE
SAFE
tiny bite of the food
DNA extraction
MobileSequencing
HMP BLAST Macbook App
Mobile Sequencer directly linked to laptop
Black Box scheme for Food Safety Inspection with mobile sequencer
• Proposed Scheme will work: E. coli DNAs were prominent even at the first hour of sequencing, enough resolution for identifying subspecies
• Save millions $$$: for both families and grocery stores nationwide• Easy to equip and operate on: three simple steps: Load Plug in -> Outputs
• Technique Challenges: shorten the sequencing time (10 mins? Is it possible?)automate DNA extraction processassuming no reagents need to be added? Poretools and BLAST functions need to be included in the software package
![Page 22: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/22.jpg)
BioTagsMobile sequencing to identify fallen soldiers
By: Jonathan Herman
![Page 23: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/23.jpg)
The Premise• Why do we need to identify fallen soldiers?
• To inform families• Religious reasons• Tactical reasons
• What limitations are at play in a warzone?• Time• Lab resources• Both of these limitations are compounded if a soldier is on foreign soil
• What systems are currently in place?• Dog tags – but what if the complete body isn’t found?
![Page 24: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/24.jpg)
Problems
• Ethical:• Mandatory human reference database• minION is not 100% accurate. Identifying the wrong body is
BAD
• Technical:• $500-900/flow cell• Acquiring DNA is not faster or cheaper than acquiring dog tags
![Page 25: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/25.jpg)
Healix
The Future of Personalized Medicine
Eunice Kokor
![Page 26: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/26.jpg)
The Post-Genome Era
Personalized medicine will provide the essential link between an individual’s molecular and clinical
profilesrisk algorithmsmolecular diagnostics
targeted therapiespharmacogenomics
![Page 27: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/27.jpg)
There will be challenges!
Cost of sequencing / each clinic might be unmanageable
Convincing insurance companies to cover these costs
Cloud system that can manage lots of customer data
![Page 28: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/28.jpg)
December 9, 2016Max Mattioli
Ubiquitous Genomics | 1
Stopping Outbreaks in their Tracks
![Page 29: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/29.jpg)
We Need FlySafe
● Serious Recent Outbreaks:○ Zika, Ebola, MERS etc.
● Growing Antibiotic Resistance○ UN declared “greatest and most urgent global risk”-Fall 20161
● No formal system of outbreak surveillance exists in U.S. Airports2
○ Current systems ask patients to monitor outbreak symptoms - wait until someone is sick then report back to CDC
○ In the days between flight arrival and confirmed illness, many more infections could occur
FlySafe | 2
![Page 30: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/30.jpg)
Our Solution Pipeline
● Use time-separated multi-sample sequencing across spatial locations in airports to localize pathogen source○ When FlySafe is used in multiple airports,
network analysis can offer granularity in determining infection pathways.
FlySafe | 3
● Align in real-time: BLAST and mash
T-00
T-15T-30
T-45
![Page 31: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/31.jpg)
Using the future to uncover the pastJordana Aviva Mael,B.A. in Mathematics/Computer Science
![Page 32: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/32.jpg)
There have been numerous breakthroughs in a vast array of
disciplines by sequencing DNA from archaeological sites
• Evolution
• History
![Page 33: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/33.jpg)
Bringing minIONs on site
Archaeological expeditions can last for extended periods of timeArchaeological expeditions may occur in remote locationsImportance of knowing the context of the archaeological site while excavatingPotential Difficulties:
Not being able to extract and or purify the DNA on site, outside of a laboratory
![Page 34: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/34.jpg)
Darwin+: Exploring our biosphere like never before
Aalhad Patankar
SEAS ‘17 CS MS
![Page 35: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/35.jpg)
Darwin+: The real animal pokedex
Mobile sequencer that interfaces with smartphone application to provide information on a DNA sample, such as most likely species, species ancestry or distribution of species in a sample
Computation and database storage done on cloud, accessed through mobile application
Fun, pokedex style way to grow a global database and identify species on the fly
![Page 36: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/36.jpg)
Applications and Challenges
For scientists: way to identify or add genomic data of new samples while on the field
For nature hobbyists: way to identify curious flora and fauna while exploring
For everybody: fun way to explore environment in a game-like environment
Challenges: purification + preprocessing of sample, using the application on the fly without connection to a cloud, speedy computation
![Page 37: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/37.jpg)
Enabling precision medicine in home hospice care using
portable sequencingJoseph Romano
Ubiquitous Genomics; Fall 2016Presented Dec. 9, 2016
![Page 38: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/38.jpg)
Hospice care & Precision medicine• >1.5 million home health care
patients per day in US[1]
• 79.1% covered by Medicare/Medicaid
• 42.8% w/ malignant cancer
• Precision medicine: target healthcare to an individual’s genetics and exposures
• Cost of WGS service: ~$1,500 per person (July 2016)
[1] doi:10.1097/01.nhh.0000263436.08062.85
![Page 39: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/39.jpg)
Making precision medicine portable• Provide sample collection and sequencing at point-of-care (in home)
• With improving quality and throughput of MinION, soon obtain >1X coverage with reasonable accuracy[2]
• Identify variants corresponding to patient symptoms without the patient having to leave home
• Dataset analysis can be performed off-site, at central location• Nurses only need training in library prep and MinION use
• Treat the disease rather than just manage the symptoms• ONT estimates ~$1,000 WGS; not infeasible for Medicare/Medicaid
• Initial funding use: purchase MinIONs and reagent stock, build infrastructure for training, and hire technicians/bioinformaticians for data analysis
[2] https://nanoporetech.com/about-us/news/human-genome-minion
![Page 40: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/40.jpg)
Targeted Sequencing with
Oxford Nanopore
Ubiquitous Genomics9 December 2016
Dinara Usmanova
![Page 41: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/41.jpg)
We want to know exactly which bacteria/virus/fungi causes the disease
Problems:• Quality of sequencing data is not good enough for reliable
species identification• Difficult to sequence many samples on one MinION flow cells
Goals and problems
![Page 42: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/42.jpg)
Technological solution
fragmentation
adapter ligation
hybridization with target specific beads
denaturation
![Page 43: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/43.jpg)
UBISeq Kanika Verma
![Page 44: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/44.jpg)
THE IDEAo The future: Mobile genomic
sequencing as a vital aspect of everyday primary care• Preventative for predisposed
conditions• Bacterial/Viral diagnostics
o An app that makes it as simple as:Connect
MinION to iPad
Obtain and load
sampleShare results
![Page 45: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/45.jpg)
ADVANTAGES AND CHALLENGESo Portable, accessible,
and efficient o Process and share
genomic data via app directly
o Consider ethical implications of easily obtainable genomic data
o Basic training necessary to operate
o Technical challenges
![Page 46: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/46.jpg)
Healthy Skin TesterShengtong Zhang
UNI: sz2539
![Page 47: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/47.jpg)
Your beard = Toilet
• Normal: 100,000,000,000 bacteria on your face. 200-1000 species.
• But if you do not clean your beard often, it can be like a toilet.
• Do you want to know the condition of your skin everyday and get a right advice?
=
![Page 48: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/48.jpg)
Thanks to our new product: Healthy Skin Tester• Using latest mobile sequencing technique
• Based on MinION
• Test your skin everyday
• Pick up the sample
• Get the sequence
• Analyze the species of bacteria
• Compare with our library and give you a professional suggestion
![Page 49: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/49.jpg)
StoolTrakMicroBiome Mapping Device
Noah Zweben
![Page 50: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/50.jpg)
RationaleAntibiotic associated diarrhea due to C.diff incurs average additional costs of $3,669 and has a mortality rate of 6% [3]
Directly linked to makeup of gut microbiome, probiotics can prevent [2,4]
H. pylori, a common gut microbe is responsible for many cases of gastritis and most stomach ulcers, and is a strong risk factor for stomach cancer [5]
In mice with colon cancer, number of tumors corresponded to the unique ways that different antibiotic cocktail modulated the microbiome [5]
Prohep, a probiotic mixture reduced liver tumor size by 40% in mice, likely by promoting an anti-inflammatory environment in the gut [5]
Cancer drugs, such as oxaliplatin chemotherapy and CpG-oligonucleotide immunotherapy, work by boosting inflammation. If the microbiome is altered in such a way that inflammation is reduced, these therapeutic agents are less effective [5]
![Page 51: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/51.jpg)
Looking Forward
As research uncovers more and more links between our health and our microbiome, the advantages and rationale behind a technology
which can track and quantify our biome will grow
[1] http://www.theatlantic.com/science/archive/2016/05/white-house-launches-the-national-microbiome-initiative/482598/[2] https://www.sciencedaily.com/releases/2015/07/150723092123.htm
[3] https://www.ncbi.nlm.nih.gov/pubmed/16633131[4] http://cid.oxfordjournals.org/content/34/3/346.long
[5] http://www.the-scientist.com/?articles.view/articleNo/45616/title/Microbes-Meet-Cancer/
Installed in hospital toilets to monitor patients’ BMTest for microbiome environment conducive for certain cancer drugsExpose risk factors for certain conditions, early warning for C.diffMeasure efficacy of probiotics on microbiome
Use seed money to develop a quick automated stool-DNA pipeline to be delivered to the mobile sequencer based off nanopore technology.Results linked to patient profile
Launch of the White House’s National Microbiome Initiative in May [1]~$500 million from pledged from WH and Universities over next 2 years
![Page 52: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/52.jpg)
Late session
![Page 53: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/53.jpg)
By James Xue (jx2218)
FoodSeq: Eat With Confidence
By James Xue (jx2218)
![Page 54: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/54.jpg)
Problem: Foodborne illness and quality- Estimated 48 million get sick, 128k are hospitalized, and 3k die each year
- Market size and potential: $782.7 billion restaurant industry sales
- Restaurants lose a significant amount of sales every year from customers who get sick from their food / do not trust their cleanliness
- Customers demand more reliable food quality
![Page 55: Potential applications for Oxford Nanopore sequnecing](https://reader035.fdocuments.net/reader035/viewer/2022062310/58729dda1a28ab07208b5177/html5/thumbnails/55.jpg)
Solution: Mobile sequencer for restaurants
- Restaurants would use our sequencers for their food to determine sufficiently high quantities of dangerous viruses or bacteria
- Empowers restaurants and empowers customers
- Both budget and luxury restaurants
- Mobile (Yelp-like) application + data processing platform
- Asking for $2 million: research and development (improving accuracy of sequencer), server space