PotejfiaBy Eiqposed to MC 252 Dischaiige Approval of tWis ...r sampling events will be completed...

Sarm ssttm SampUng Pfata

PotejfiaBy Eiqposed to MC 252 Dischaiige

Approval of tWis work plan is for the purposes of obtaining data for the Natoral Resource

Ihe tnistoesiMxe deyelpi^ a I^limin^

the decision iQ pumie thfrStudirs outlmcd in thearKi^eei%to fiuultfae w6r not ehdorsihkflje tnodefa^Sildip | g j j _

APPROVED;

OAATtosteeRep

tee ftepmentative

Irastee Representative

/Date

l J< r utOate'

( 2 ^ - 3 - 1 0

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DWH-AR0017415

Sargassum Sampling Plan

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DWH-AR0017416

Sampling Plan

'Sommary ^

This document presents an assessment plan (Plan) for juvenile sea turtles in Sargassum. The

sampling planv'Since time is of essencerthis plan^is, a study to b e :

r H. Data heeds and sources. This section'pfovides an overview of the types o f data that will 1 : ; be needed td complete the assessment of S'arfaA’SMm and of juvenile turtles.associated with\ ;-these;5rtrg#s*im:cpmmimit}es.^ ;,;v ,-rt ' -p:: p; j v 1 .

n i l f H e a t t and Safety. This section Mttmarizes health aM safety protocols-that are relevant' rto this effort, l i t includes a number o f procpduies: by reierenGe,;allrtf which should he . 1

I¥ . - Sitehelectfph-sfrategy. Thw sectiQn describes a'proposed approach to identify sites for ; ;viTf;evaluationltt---f f-;t .■ '''--'.f-v't ;;/f:

■ r¥. Site proeednrfes. 'This section with the; sarnples and dafk

procedures, whether mcluded here d fe or by reference.

'v i . ;; - Operating procedures (SOPs) proposed for use during the study. Mnciudes specific . ■

Vi datasfaeets/foims to be c(:: protocols:anti;&mis.; ■

DWH-AR0017417

.yargaysM/w Sampling Plan

1. Approach and rationale

Floating Sargassifm is a key oceanic habitat lliatmay have been impacted by the of MC 252 oil and dispersants (hereafter referred to. as “MC 252 oil”). Sargassum p ^ h s s in the Gulf of Mexico are formed by the convergence and aggregation of two species o f brown aJgae: Sargassum ruMa)is m iS , fluUans. The GulfofMexicD regionmaytbe the second mrjst prodi^ SaVgaysMm system in the world (Gower and King 2008). The pelagic brown algae is an oasis in the open ocean that supports an asseniblage of marine fish and invertebtales.

Because patches (which may be mats, cluiiips, or convergence lines) are,found in the neuston (floating surface layer of organisms), these habitats and associated fauna were and may still be potentially at risk of exposure to surface oil, sheens, and chemical v; dispersants introduced as a result of the MC 252 discharge. Although shipboard observation of Sargassum patches can be traced back centuries, little is known about the life histor}- and ecology of floating Sargassum patches. Until recently, it was assumed that Sargassum r ; originated in the Centra! North Atlantic Ocean and was advected to the Northern Gulf of Mexico (nGOM) by surface Winds and ocean currents (SAFMC 2002). Recently, scientists examining satellite imagery have questioned this theory and proposed that the opposite pattern occurs, i.e. Sargassum originates in tlie nGOM and is advected to the North Atlantic (Gower and King2008). Given that jfloating Sargassum plants may live for two years or more and the nGOM may be a nursery' for the algae, the effects of the MC 252 oil may potentially extend beyond the nGOM.

Sargassum natans is far more abundant than S.fluitum, comprising up to 90% of die total global drift macroalgac. Limited quantities of several other benthic species, including S. fdipendula, S. hysCrix, S. polycerliwn, S. plaiycarpum. and S. : ; pteropleuron, deUiched from coastal areas during storms, are also frequently encountered adrift. How'cvcr. the drifting fragments of these bentliic species soon perish (SAFMC 2002). The pelagic species are golden to brownish, in color and Typically 20 to 80 cm in diameter. Sargassian has a complex morphology in which the thallus branches into stripe-bearing leaf-like appendages (fronds) and gas-bladders resembling berries (pnciimatocysts) (see Figure 1). Gametes are produced on special branches called '‘receptacles.”These, in turn, have pits termed “conceptaclcs” in which the ova orspermare produCed. Propagation is achieved predominantly via _vegetative fragmentation. As Sargassum ages, the plant darkens, the fronds become heavily encrusted with a variety o f life forms, and eventually, density exceeds the buoyancy provided by the Sargassum's gas bladders, and the algae begins to sink.

Floating Sargassum patches are an important habitat for a variety of invertebrate and vertebrate species. In particular, Sargassum patches are a key nursery habitat for four of the snecies of sea turtles that inhabit the Gulf of Me.xico; loggerhead (Carelta caretta). green" T--__• HIM mil INI . '' ' —...................................................................................................... ............. ..............................

UT..'.

Figure !. Diagram o f Sargassum natans.

DWH-AR0017418

Sampling Plan

(Cheldnia tnydas), Kemp’s ridley (Lepidocheiys kempii), and hswkshill (Eretfnochelys imbricata). As Sargassum clumps and mats ai-e concentrated in the nGOMj these neustonic habitats and associated fauna may have been potentially exposed to surface oil, sheens, and chemical dispersants introduced as a result of the MC 252 discharge. If is anticipated that the overall evaiuatioB of i ’argas'sam. w i! include invertebrates and fish of several life stages,

The main objectives of this study plan are to:

: (l)13etetKline the areal extent and distribution of iS'ofgajs'um in the north-central and :eastern Gulf o f Mexico, and its spatial relatiohsMp to previously observed; surface oil and

1 .dis^rsaats associated with the MC 252 disch^ge via aerial surveys; and-:

,(2) Eocumertt the density, condition, diet, m d potential MG 252 oil exposure of pel^^^ neonate sea turtles associated with floating in the north-central and easternGulf of Mexico, and along the southeast coast of Florida.

■ - ■ . ; ■ ■

■■ 0

The trustees aodBP Exploration & Production, Inc.. agrees to fund, 8 sampling events extehdMg into 2011, and it is anticipated that;up to 2 of these ■;

r sampling events will be completed during 2010. The timing, frequency, type, and locations of turtle surveys to be:completed in 2011 wilj be based on % observed conditions of the spring-and early summer of 2011, ;as well as the seasonal distribution; and movements o f ;

across the study area. In addition, the results o f the 2010.samp}ing:effofts will be< usedtoevaiuafe whether any changes in approach are W’arranted for the 2011 surveys. Any ,

i changes in approach will be documented inan addendurn to this plan and agreed,upon by BP and f, the trustees. The trustees will submit a budget for the sampling events to be conducted in 20II .asan.addendum.to.thisplani,. , ' '

StudyA repy y • ,v'■ vt ' -f .'.v ; : y ',::TT. -y, , I f, A ' v . ■ .-V-I V'-v.'. : , ’ t ■:> ■ Principal study components of this. proposal currently focus on areas potentidly impacted

By MG 252;oileartof toe Mississippi River Delta and extend to locatipr^ along the Florida y ; : 1 I I f:; ,Panhandle, and to areas off the shore o f Florida where pelagic, neonate sea turtles occur. As holed :above, sbiehtists have recently proposed that &rgd5y«Bi origirtates M the hGOM. and i^ then advected to the North Atlantic (Gowpr and King 20Q8). Given this new,understariding of argrt#»m , 's' possible patterti of movement, that potentially .came in contact with MG

’■■I. ' 'V,'

i, and toward thev Atlantic coast of Florida via the TUiaj lHUVV UMMUg... w-l,. . ,

northern Loop Gurrent .(which moves torough the GOM and into the Atlantic Ocean). For thisreason, the study area for turtles is expanded to the eastenj fiOM (off Sarasota County, FL) andalong the southeast coast of Florida (off Brevard : County', FL): The .Brevard Count}', FL area ;may be oceanographically closer to the northern Loop Current than the Sarasota area, making itan appropriate area to examine fa t Sargassum. patches that may have Originated in die nGOM.

. Even if a patch may no longer be show'ing visual signs o f MC 252 oil exposure,

DWH-AR0017419


in

maximize the probability' of eneoufltering post-hatchliag sea turtles, Brevard County has the

j on the gulf coast of Florida. Choosmg sites in

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to evaluate poteMialipjui^:bassdon'a/surfK oil;expbsure.'3T® study design is InteEdedto^b / i t v f f ■pirica! or modeling-based ihj'aiy assessment

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ly. H depicts anexsniple o f a series ofthe:typeof:

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ve been, identified to; represent : , . ; . : F,rvey.griaS'are;idcatedMtie-GQ .f F A

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ving encountered MC 252: oil; However, a ; will be determined durmgeach sampling .syent y. ,

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and turtle biological sarapies.

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DWH-AROO17420

Sampling Plan

Sargassum Locations

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DWH-AROO 17421

Sampling Ptaa

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DWH-AROO 17422

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DWH-AROO 17423

5’arga5'$«/w Sampling Plan

B. Sea Turtle Sufvevs (Qbiective

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Aerial Mentifiea^on o f Sargassum is a means o f locating juvenile turtles (see (1) below). Turtle surveys vrill be conducted in four survey grids located offshore of Venice, tA ; and Pensacola, Sarasota, and Brevard County, FL. See section VI for site selection details) This Plan

are being studied lbrough other NRI>A plans. The sampling program is designed to obtain samples at three types or classes of Sargassum patches; mats, clumps, and convergent lines. Following the aerial surveys, a number o f potential sampfing targets will be identified and subsequently classified as mats, clumps, or convergent lines of Sargassum. Within the aforementioned testing sites, the following wili be accomplished;

■

4]^.SeaTi0leSi{rveys : S

On 28 April 2010, the NOAA Southeast Fisheries Science Center (SEFSG) initiated a series of aerial surveys to assess the marine j aquatic areas that cbuld be affected by MC 252 oil. those sea turtles that have carapace length greater than 33 cm. The aerial suryey data collected to;

loggerhead, fvemp’s ridley, and leatherback sea turtles. The spill region, however, is known to contain juvenile, pelagic-stage sea turtles of four species: loggerhead sea turtles, green turtles, Kemp'’s ridley turtles, and hawksbill turtles (Witherington and Hirama 2006 & 2010, FWC

i o

DWH-AROO 17424


2008). Most o f the youag turtles are 25 cm or smaller in carapace length and are not easily

'

turtles-(FWC.2008)., , , ■ ^ . i / h f -'

Oo 17;May 2010, the Florida F i^ and Wildlife Commifeion (FWC), in conjunction with ; ; ■■J ' othergoyemmentageneies withinthe Unified Comin^d,; initiatetfthe c^ tu re of oiledaea 1 ^: inhaWtingpelagie&rg«55tiOTpatchesnearthei5egTO;aterifoffzaKwelihead. A so l 10

;reenturtle,ft . i f ^ f ;lDggerhead,;aiid:hayfebiU'(listedin;orderofpre¥a1ence).:Vv W it ., i : f... 'i.i; i 'o f t i ' f :f f . : / ' C '"i' -iV:)!; ■ î:' 'W ■ - > ' C . y i . W ) / , - ' , ;/-■ f y oij.ii:

i i y f t i s Flahcoinponert isdesignedto augmenttheaerial and;on-shore;seaturtlesurve^^ 'iconductmgaFsea capture of sea turtles to estimate turtle:dens% (se# Site Procedures ;iQOIlQuCHng SX'bCo; vdpiUlC Ui bva tultlv^ tv wc>tiiiiai.w ,vuivi.V;iuviicjiv ^

■ , ; Qyei-farge areas.;Fhe turtlestar^ted arepelagic|u¥eniles;andpDSt-}tattdilings. Fpllpwing aet^" > isu ivevs;a ll:& ?ga«h»:pat^ obsdrvedln a :^ d witl>be:classffiedfemats^'e^^ iy / : y y )

y; : 'v. :v;: V conyiigentlhesand afargetiaieawithinthegnd w ^ AFsea, ,\ ■ sampling: of turtles-wiffoccuf from: a A altpg onpor rnprefrahsectSthrtJUgh tliey: ^ ^ ) y f:

■■ / ; ' targeted Fhe suhjecttransect will follow o f paralfoLthe long of the selected - - y/. : y, ' V fegassampateheSfisimdwithinthetargetarea, ObsertftrslocatedpttAeShipwillrecordy: y ,-y y / • : positions'ofalhturtles observed along the t ia n ^ ^ lineând:eaefctortle’s petpenditgiM^ yy-y.

, : f y y y ■ : ,y Jfointhe trarisect.; These observations will be analyzed inôneiof ;several: ways, sucli-a y y

-■Site:'--' -'^■^y:.:C^fyyy:fy;Procedures), yTurtles observed along the transect centerline wiliybte collected using tong-tmndied, yriUVCUUiSJSJ. WlV-lig VliV/ - 4 7 4

dip-nets after the distance off transectiat which the turtle wasysighted:is recof dedr Dietarysampling and detection of external and ingested p€troleiim,: if any , will be performed by

'compefent individuals trained in turtle handling techniquesc. The research teamwill possess alfy y, applicable and^fetiuiied state federal pemutstofmplement thidFlam: ;" ■-y: ,

iT-.f-y.-y-:-.

/ y t ' y y^^: ifanyfflem berofattiteatenedorendangeteaspecteSSbQ^ signsofrecentoiling;^ y .yy yyy will be:teanspbrtedimmediately:to port: arid an appro|ttiate-rehabiIitation:fecilhy. :Arrangeme!>fe:y y y .

for txiriôrtatidfe-aBd W & hichfk:ilities for each port, wMfbe coiripleted before each ..................

.V :event..:,: yyy- ■ :y y-:yyy.. -'TTy.-- W''- - ' - - - - - - - - - - - ' - - - - - - - - - - - - - . . . . . . . . . . . . . . . . . . . . . . . . . .. .

: (Sj Contamingm m ' i y : f T . y )

■ ■ samples ahd plants collected during, the study. ySpeciffcallyytwo replicate sam pte yy yA suite oi,cx>ntaniinani analyses wiu DC periumicu uii baiiipics iiuiii

samples and plants collected during the study. -Specificallyy two replicate samples,o f tissue (75 g y . :each).will be collected from plants ateach patch sampledyandpreserved at-20“C for ; y

:t ■ - Kp> fni*HuHrnf)«rhr>ns y, . ■■■ ,y , ■ ;■ ■ '■

source 0 1 any jpetroieuin products, rorensic m geipnni anaiybib wiiiyuc A.uiiuuw<u ^Sargassum' collected, -unless:.such'an .analysis ■ is not technicaily .feasihle. TurtleybiplOgical

ucted on any oiled y T y :

Ty'.' -y-.-.y:"yy--.I f ' T ' . ; - y . .- y . .. ; . .- :. ...:. .y ..11. 1. . : . .. . . . . .. . . . .; . . . . . . . :.... .. . . . . . , . ; .

"y ' - - .:-yfy f- y-f-y"-: F'y '-w'".-

. ' ' - . . / y - . ' ; - ' : : ; : - - . - ' y : ; ................. ;'yT.y;y-:y'yy:....- . - T- t e . ■ ■.. ; . '-y ' \. y..y- .-'.y.f--,.--. -.' i . ' ) - y.‘- - . . ; . . ..' -.y.yy. ■ ..y y- . .: '

DWH-AROO 17425


samples will consist of carapace s w a b s , esophageal lavage, and fecal sampling.; Analyses of these samples will Be addressed by a separate

1 : ■ , m .:;liealth and Safety ■ - ; . ,

* ' W3A or state agency oil spillpolicy, ;;; v;

vl'i-'-f' y - y .

- v y y ; > i - -

252 inciderit” (NOAA;

y : ' V,, W \ "read all o f the documents i

■ *-

" v ^ ' f - A y y y i : i'■ ' ' ^

’s site collection activities < with the safety documents f

■vc.y :v :

■V-; :;.y:y'»y;v t A i y , " :.V-

yr. y y : - y y , .-^y ^-^Ai;y; ,yy:;w::;yy. ,yoy,

°. ■• V . ■ . ■ •:-.V, ■ ■ ■ —

,

.:y-, : o .

'■

.

■f-'" A y“

. :J-t V , . V ..:. - - .

:yv;y-. ' ;2.y

^ , r . y , r ; . y

h.'.r,- -> ■■ -hy^^f :■

■ y : yA' ; _ . y ; , - ..

: ; y y : t -pl.n, no(laM th.,ithenightpiiorto gOTO . ; ; ; .

y;- " r : i;: - : ^tbers; ' ' ■ ^. . :vA. ,v : ;y y :y :y ::yci .^

\r'- -npung they are doing;;timyat sampling area (daily): ; ufefrdra^samplmg area (daily);,

y ^ . y f : :

y;".-' ■ '■.■'v 1'

,

-y

: . |y ; y

W'h- .

■ /■ ■■ ■'■■ i-'yAy A y . ' f . w y - y ■■■yyyh

^^J;Fleasensepnelab;foreachreaoi.w' -\_p; - r ■ )>'h ■subm ittt^lecttof^lly, the :A'./wy:y,.,

' ' . . . .'

I : ' f ' : h v . i v : - y :

ai-lyprGoedures seifotthiri the M € 252--Site.Safety: P ■ /■,. lAi 1 A

.

'■ ■ ' A ■ ’'v . ,

' . / i■ r..'■ .cy:';-;,

w . - :, -y., ' . ".' : ' ". ."' : ■ .. ' y; - ■. '. '

Vi ^ 'A t-y ■■ ' V V-V y , yv y . -v, ...

, ■ ' f:..: ■ .wA-‘A'"i-

■ : V-:.' ■ ...'■ . '

■ ',■■ . ■..■■■ V. ,;::: . C;:

. , .V ■'■/ ; ■", '

. . - y ——— V ^ ■

v ; : •;: — ' ' , . ■ ■' ' ■■ /

DWH-AROO 17426


"

■■■■, . .. .. , ^. . .

' , ' ■ ' - '■

' ■ ■ . ' '■ ■ , . ' . , , ^

; V :

IV. Site.Selection Strategy ■■

proposed in this work plan is

sfi thisi Therefore, in iselectmg the ■■'.■, t-'i ^.;:

. . . , ,

as a whole.might:compose a"gradient of poteHtial oiling: Thus, for planning purposes,: it is assumed that W . I Saw a hlohftr ntelihnoH Of encouTiterinE MG /

fTensacola, FL isioticipatedio' iail between these sitesfh potential for im¥ing enccniirtered MC; 252 oil. A

visua

The day-prior toplaimed boat:sampling trips,:aerial surveys oftfae;Subject^^^ f i ' • -t J UA rtf flhA fîir VenicecT^

' ' ', : ' ' - F ' A,' . ,, ;/i: A • - : •

CGIlQUCtvU i\cliai gliU 2>uivc;^3 Will uw ----i-—-• , —'Pensacola, Sarasota;an4BrevardC0unty,FLwDarmgaeriaisurveys,fi?edt^^^

j ’ .j ,>rr*-cinci»/.t:.tictflnr.es,r§ize'Classrtype,etc.):'will OS . . -:■;

:aM:typedâs either mate,:clumps tJswBsicu, :«iiaii agsiv^MM*« --.-raggregated into distinct lines or mats), or convergence lines. Following aerial surveys, the ■ : r Wrrlri ei-t nf ferir srtrvevAlocfts wmeriiilDOseâggregatea iniO ulSlin^t lllieb U1 li ia ^ yi vyuyvigwiivv Vdbsereed jSargo SMW patches wdlhbe pibfted lQh a set of four Sdtyey hlpcks^ superi on the

: • 1 / -L , TT-i;. rt\ Ofid tilft tlllfftHsr o f tVt)SS of*

Sargassum patches fGuad (i.e., matsa-particular survey block, the count ' ,A;- A-::,example, ii iwu typcb Ui A-I---—-7--”- t : ; :

:fbrthat blockwouMbe2.::PossibleGourrtsare 3, 2, IvahdP; The.}istpf4SutveyblQcksfbr:fte. ■ ;hridwill be sorted by numberof 5rtrgi^rtfK lypesTresent. -Tomaxim^ ^. Ar- t 1 » _ :!.■ 2_. *11 wi.rv, l»-o +ko fr-rsrtct cirrl trtfl t\/rsf>s aiirf:<nitaHlernatchcS orSargmsnm: If or more _.. same number will be chosen at rahdoitir

A:-Tf:A,; y,A- -

fT;::-'::A::::;A:,;; .":a :;-A;.;,;;. ::,

r.. ■ ■■ ■ ■

'

d" bldck :The; aircraft will join the nffbrt ;and will fendeswous with ihe ship at

A;Workitig;M,; 'work in ( Ay:-;::A:::;,,

deems nearest the currentôsition of the ship andthm is of sufficierrt siê to If; t j A; ; ,;y.allfhreetypes of patches arenot ayailabie to sample within a: grid, t te F; ^

; ; : - : a

■;A.,::p-;Av- M .

-.a-F-:a :a '-a','A.'.--' . -This 2010

cost-effective data collscdon o® ; :'Based: on an evaiuaiaon oime. .toiu sauipuHg v,!ui

pproach: are w'aitanted for surveys that wiil be resiiutiatedin 2011. The total.numpeE Oi:

.::::: j : :

; ':'\r. '■Ah

A' . •■ ■ ■ . •

DWH-AROO 17427

S'arffasyaw Samptigg Pian

Sargassum patches sampled duting 2010 is elasses/grid x 4 gfids x 2 sampling events).

x 3

\f. Site Procedures

capture and release in order to assess condition, diet, and potential N4C 252 oil exposure. The metrics associated with these sampling elements 1 -3 are detailed below in Fable 1. Table 1 also summarizes!sampling events extending into 2011.

Table 1: and 2011

ComponentAreal estimates Coverage Aerial

surveys in each of the four grids and vessel guidance on the day of tire survey

Dependent on field conditions

N/A

ContaminantAnalyses

Concentration Collection of up to 96Sargassum plants at each patch in each grid - up to 6 samples per grid (generally two per Sargassum

Turtle samples - esophageal lavage, fecal

carapace swabs

up to 192

patches x4 grids X 8events x 2samplingreplicates)Sargassumsamples.

DWH-AROO 17428

S a mrassani Sampling Plan

■: /

V- :'

'.-: ..... .. ,

■» - : :■: ;:; -I ;:^ - : :/: ;r " : ■ : - : : ; : r :

»TuMes^ ■ ^ Dmne, Speofes- ' 1,,f_ i,tiixi0S . ; ■ i-'Cnsixy 3nci. l i iicx - pvy

■1440 Turtles,

' '■ ' , - '-■ -'

■ V ■ ■:/

■■ /'■ ■■■■ ■_ V,V ■ : ■

' ■ ■/ ' '/ " ■/. ■:■ • . ■ \ T

-

. : j : ;

1 V , :■ :>f

N o te th a t"

:-; r - ■ ■ : : . : , ■- ■ ■ ■. ; ■ ",. . :■ ; ■ : ' ■ , ■ ■ • ■ ■. ■ ^ - : . ■ ■; : : V , ■ ■ , , . : r - . : ■ /:■ ■ ■ /■ ■':"

f~ rr-— -------------' '■' r .; j . ' ' >' v>.‘~

■ 'T.;v.,. ■■■:■

■ ■ ■ ■

, ' •, -;:VTT 'TV/.

.■• ■' ■

,- V - , ; - ,:;■\ ■ ■■ - ' - T ' . -

S'-"'

Following the aeml surveys^

Section IV ~ Site Selection Strategy). attempt to rendezvous at the designated center point arid proceed to work in coordination to

DWH-AROO 17429


identify and sample thtes Sargassum patches, idealty pne of each type —mats, clumps, and convergence lines. At each of the three Sargassum patches, ship-based observers will record

; dimensions or perimeter coordinates of the patch(es). Scattered clumps will be measured in diameter by recording GPS tracks along two bearings at right angles to each other. Mats will be

Pleasured using the method .described above or using a inclinometer and kiiowin observerheight. Length and width of cdnVergence lines will ise obtained using a GPS-measured track.The Weight o f Sargassum sampled, its condition (extent to which it is w'cathered), and potential MC 252 oiling evidenGe will be recorded. Up to 6 samples of (75 g each) will be . ^collected from each; grid. Samples will be stored in aluminum foil on ice for no more than 48 .hours after sample collectiGii, at which point they must be frozen, at -20°C for analysis at a later

. / d a t e . ' . / V ; ) : .' ., .b . , ■ f ''.f ; ■ V."' . A ^ y

C. Sea Turiles / ■ :

: Sea turtle surveys will be conducted offshore in four sampling grids—^Venice, LA; iPensacola, Sarasota,, and Brevard. County, F t . Each of these locations has a record of pelagic: ); ■jiweniie seaturtle captures. Surveys wiHbe conductedusMgfrainedobservers. ;.

" ■ PotlQWing the aerial surveys, ships conctactiHg the turtle survey work will proceed to the i . center p Q i n t of the block vvithin the^g based on tlie results of the aerial survey

.; observatiditeif^ described above in Section IM -.S ite Seiet^on Strategy)^: The turtle, survey i :■ shipsiwai woik in coordination with aircraft. ) A ; ; y U U

: . ; :At-sea sampling ofturtles within a grid wiiroccur from a ship conducting a.yisualsearch:along one or mare lines that transects eachfarget identified by air support. Each line transect will be parallel tQ the long axis of the tsxgot Sargassum patch, with the search direction (e.g., to the.

a coin toss. Transect lines through scattered Sargassum wall pass; through the densest patches (assessed visualiy by trained observers). For each turtle enco:untered, pbservers will record the :y position of the turtle along the transect fine and each turtleTpeipendicuIardistmiGe from th e .

, tmnsect line. These data will be analyzed by pnertfseyeral methods, such as using a modelthat (.incoiporates these dislances (the: prograni Distance 6.d);tb calculate detectability,: effective ; / ;;:'transectrtripwidlli,:and:turtte;density(TKomas;etal.20i0;k: y y ( : : y. y

' f : : Observed turtles will be captured usjngylong-handled:dip nets. Turtles will be capftired, : ; meMmeaiexamined, tagged (PIT andfripper mgs), and released.TEkaminations will indude y f ,

.: external'swabs for detection of MC 252 oil, an oral examination (and^swabs, if necessary) for tar, and an esophageal l avage followed by coUectiQii o f feces, taking advantage of the gastrocolic. :response,to lavageyDiet items will be: MeMified and:any suspected MC 252 oil will be sampled, ■Swabilavage and fecal samples will be collected; using methods that include measures to prevent salnple:coiitamination. If any captured turtles(are not vigorous or are within visually MC 252 piled habitht, they.will be captured, measured, exafflined, and; immediately transported to thp closest rehabilitation facility in accordanoe with procedures established prior to each sampling

; trip.yThe capture and handling o f all turtles will bs.con<iticted.by;Competentand trained :

.161

DWH-AROO 17430

Sargassum Sampling Flan

biologists in compliance with the research team’s state and federal. pennits authoTizing the researchactivities', ' ' '

.

; the Serlrajj-uw collection and turtle observations/coiuits. i

■■■\ , C a t a f f l a n d l i n g a n a ' S M r i n g ^ ^

V ; MC 252 NRDA chain-of-custody procedures will be observed at all times for all NMDA : :. . . . . . . . . . . . . . . . . . . . . . . . . . .

ivxv wlii uc UU5CX vcu at i:tu umca iur iiii i t . i t t Sainples. AH samples wiibbe tra n s i te d with appropriate chain-pf-custody forms.

; . i- : .t t : ; : : field and laboratory data will be collected, managed and stored in accordartce: with C i i . b iV ; . written SOPs. The appt-opriate traihing'dn particular equipment or in the conduct o f specific

;; i ■ ; ; ■ studies for ail persormel m^ -

Each laborator}' shall simultaneously deliver raw data, including all necessary metadata, generated as part of this work plan as a Laboratory Analytical Data Package (LADP) to the trusteeDataManagement Ieam (DM13, the Louisiana Oil Spiireoorditiator's:0ffice (L0S€O ) :

L'y y cffibehalf o f the State.of Louisiana.and to BE (or ENTRDCpn behalf ofBP). The; electronic data ( / ■ fi-i ■ L deliverable (EDB)^spre8dsheet-with.prewaIidated analytical results, which is a component o f the; :.

- V : complete LADF, will also be deliveredrto the secure FTP drophdx mairitained: by the trustees' ' . ■ . : - : i .■ ; -Data Management Team:(DMT). Any prelimfflary data distributed to the D

4->s T I>n r?Xl'T'T>T'V‘A /TT-'

W ii u v i ia .i i ;A Jx u i v \ , ; i J z t ^ u i a w i a a i i u 4M JLvix

deliverable (EDB);spre8d.sheetwith.pre-vaIic!ated analytical resu]ts,vvhich is a component.of the ’complete LADP, will also be. delivered to the secure FTP drop hbx mairitained: by the trustees' :B ata Management Team:(BMT). Any prelimfflary data distributed to the DMT shaH also be .

; :. ;-aistributed;toLaSCQ andrtoBP(orE!TrRIXonbehalfofBPL Thereafter,the D . / \ i t, \ validate and perform quality assurance/quality control (QA/QC).proc.edures on the LADP L

. A, eonSistentwith the authotized;AnalyticsIQuality Assurance Plan, afterwhiclitime the Vvalidated(QA/QC'd:data shall be made available simultaneously to all trustees and BP (of i:. f i L ;: ; , /ENTRIX on behalf of BP). Any questions raised on the validated/QA/QC results shall be

. handled perfhe procedures ift the AiialyticalQuality Assui'ance.PIan and the issue and resuits . ;-'.'i "

. . 0 shall be distributed to a i f p ^ InfHeTnteresfofmaimainmg ottecdfisistemdata set fQr;use by , :........ : : o i l r»orfJjP»o rtm 1 \r i r a l J /lb if iiirJ /rt-A A / l o t o c ia f ?^lei> ooa.ri Ki:» fW a , .....: ■ y;:^ only the valMatad/QA/QC’ddata seti^Ieased;by:tteDMT:sWl be consid

L bo^sensugclata set. In the interest ofmamtaining one consistentdata set for use by all parties, fL dnly the Validated/QA/QGM data set released'by the E M L sh^ be considered the consensus data. , t L; ('

( ; - set. m order to ensurereliability ofthe consensas data and M l review'by theparties,mo'party ■ ■f shall publish consensus dateuntil 7 days after such data Ms beeH made avaiiabfe to the parties,

y ; f ■ ' A}so,the LADPshall notbe«Ieased:by the:DMT, LOSCG, BForEhOiyXpriortO:f. ; ^ ; f ( ■ L i ^i.validation/QA/QC absent a showing of critical operational need. .Should any party show a critical .■

: nnemt ional need fnr dMa n r i o r t n validatfori /OA/nr ' , , RTiv mlftaSfid data will f e f'.iOflT-lv

■ . - \

DWH-AROO 17431

SargaAyam Sampling Pian

ENTRIX Oft IjeM f of BP).' to all trustees and:to BP (or

t lO Q -J

ies. A lf ffk. AO analytical and

reasonable timeframe. 'AH samples an IfRDA-approveddaboratory.;

Research! Institute

Dr.

'.)Descri{3tid9;«f Duties ;forXead'Iflyestiga1^

'.'/■■'■'I: .'"r . . "Role':- ' v ' - ; r r / l - .

.D r.B lau- W itheriagtbD : '

Florida Eish and Wildlife G onsem ^Commission ' 1

Lead Investigator for Sea Jo'rtl'e ; ' i Compoiient- Responsible for traiiiiilgiield staff development o f data-col lection :

.p rp to co ls fy ,Dr.:JotaatM#n GdrMairf' '. , :ihWater;Researe.h: Group:, file .: -

Coilnvestigator for Sea Turtle eotaponeat. : ResponsibteTbr procuring eqiapmenfld^ / collection; analyses, <Hid leadingTeport . preparation;

;:Dr. Mieftael Bresette:, . l&WaterRe&a«di'Gfoap,TM^ 1 : I ?

Co-Investigator for Sea Turtle :Campdnent; : Rpsponsibie fcf p rdcuringddU ip tt^ collectiofl, analyses, and leading r e p o r t ; :preparation. "

DWH-AROO 17432

Sargmsum SampBng Plan

References

. Craig, B.A. and I.E .;: Atlantie coast oWlorida Ming aB ay esteap p o ach with t to |» a ta re -^ ^

: Gower, J.F.R.,;€,H«;'G;A.Borstadand S.. King, 2006, Ccean colorsatellites show extensive ;;: ; . - ^ lines of floating in tMe Gulf of Mexico, IEEE Trarisactions on GeoseienGe: ant. / t . Remote Sensiiig,:.44, j619--3625^f.t.

Gowtr, J, aud S, King. ;2008. Satellite images show the movement ofBoatiag SidfgaiXMOT m

'.■Hougteon, J.D.vit,K. Doyie,,M.W.: Wilson, f.BaveflpoS,: and G.C.'Hays, 2 0 0 6 ^ : aggregafions'ahdleatKeiBack turtle

rolina. 153 pp.region, s

Thomas, L.,:S.T. Buckland, EA .'fexstad, EB , Laake, S: Strindberg, S. L. Medley, J. /: Bishop, T. A. Marques, and K. P. Bumham. 2010. Distance softw design and ;

anaiysis ' of distance sampling surveys for estimating popuiation size. Journal of Applied ■ Ecology 47; 5M 4.B O Irm iJtl41365-266A 200^ A y

■ W item gffin B. E. 2002. Egologyiof neonate loggerheM turtles inhabiting lines o f dqwnwelling:: near aGulfStreOT'ffoiit. MarineBiology 140, 843--S53.A ; r : r, :y f y ■ ; ;

' Witheringtoh, B; and:Si M Sea turtles of tfie^epi-pelagic drift comraanity.': ; A ;1 In Mi prick. A, Panagopoulou, A . Rees, and K i Williams (GompilersT Boifc o f Afestrtcts,,

:26th AMual Symposium on SedTimIe Biology and-Conservation, Book o f Abstracts,

WitMeringtdn, BiAand T.Hkama. 2010. Oceanic-stage Kerrip’s ndleys from m.e open/watefs of ■ " theG ulfofM exico.Pp. 143-144 in Dean,K., and Lopez Gastro,M.C. (Compilers),^

DWH-AROO 1743 3

Sargassum Sampiing JPlasan ^

E s t i m a t e d 2 § 1 § B u d g e t

.: Aerla! Surveys -Turties = ;' Charter Aircraft

Inwatsr Research Budget ■,SWp:^Based Surveys-- Turtles " ;EC(uiprnent

Unit . . Costper month , ^ $123,424

# months

le ■. :- PITtags and electronic reader:(2S.taigsi!$750rl^r @$250}: 'Veteriftary lavage: purn ^

■ ;Samplejamahd,pl:ese^vat^ve,, " i; V.' ., , i Data p9pe0offlcesupplIesi:T ^ ^t ,, fli.gauze^aiTipHngsuppliesv■ ;.SateHilephoHeminuie5$$;

■ Vesselexpenses:: /■ chartered vessels^ [16 days -'ajlow/ihg up to 2 days per grid- i , [inauding contihgehcy for badweather] >t4 grids,x2 events .

//'i

:-E9Uipitfent;S0btpta(-',-, / ■ [ -

■PersoBhel-expenses-.;. .[-'r:; -- T r / y - - -

; '32 field weeks plus prep time

/:RrstCrew - -- Chief Scientist

’■ /Research staff ■i Second Crew.' Chief Scientist )!

. /Research sta^

E>ata Analysis and Beport Preparation ■ / Chief Sciehtfet V///!// . / ! > \ / ..

PersoBnel.Subtotal. ; .

Rate hours/fflorith/ f tnonths

II

/I/.Total.cosii: :

$123,424,

^■:Sl23;424:

:$i,£5oq.//$350..'./$15G.",$125/.-

;/'.$125;

/$56,000;

II

/Tottl-cost;

■ ■ $21>090-.i ./'-'■$2$,133::.

/ /$21iQ90:,

V / Sl,S06

t. $93,982'/

Travel

Sa raso ta, FL .Lodging : - : Meals ’

/ Rate/ : .Daypifllles

$86.00i$56.00

7.J

People.

■:/'■-. 6 'is-'

-Total.

$3,612/ .[$2^352'

DWH-AROO 17434

Subtotal S ebastian , FL :

Lodging .

”5 :

Sargassum S am p lin g P lan

SO.SO : ,750 . : 1 - $375

■■-'■■■ -S '' ,ti

7 . ; / ; : ''vv ; /v

■ '. '. '

. .

0 0 - 7 . / 7 . , ,, .

y^SO ■ ^

$7i.00 - „ V' , ■ 7 - : ■- ..JSOr ■ - 1 ■' >

: ■, V —

\subtotal , .

™ i f O , S h , p S = » J 5 . ™ , s - T „ , „

$6,339

$4,158

- ' ...

'■ . ' , ' '

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