Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein...
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Possible factors affecting ribosomal protein expression 1. Autogenous regulation of protein expression. 2. mRNA protein degradation. 3. mRNA secondary structure. 4. Missing factors in E.coli S-30 extract
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Transcript of Possible factors affecting ribosomal protein expression 1.Autogenous regulation of protein...
Possible factors affecting ribosomal protein expression
1. Autogenous regulation of protein expression.
2. mRNA protein degradation.
3. mRNA secondary structure.
4. Missing factors in E.coli S-30 extract
100
75
50
37
25
15
10
S1
S2
S4
S5
S13?S16?S?
“Specialized” ribosome
GFP X W X W
Ribosome N N S N
AGUGTAUUCACAUA
Future Steps and Issues
1. Increase translation efficiency.
2. Reconstitute 30S and 50S subunits.
3. Genome organization.
4. Autogenous regulation.
4. RNA processing & modifications.
