Population Comparision, Germination Rates, and Inbreeding Depression of Limnanthes floccosa ssp...
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Population Comparision, Population Comparision, Germination Rates, and Inbreeding Germination Rates, and Inbreeding Depression of Depression of Limnanthes floccosa Limnanthes floccosa
sspssp grandiflora grandiflora
Julia McGonigleDr. Robert Meinke, Dept. Botany & Plant
Pathology
Limnanthes floccosaLimnanthes floccosa ssp. ssp. grandifloragrandiflora
State of Oregon Status- Endangered - Oregon Natural Heritage Information Center List 1:
Taxa threatened with extinction or presumed extinct through its entire range
• Federal Status• - Federally
endangered without critical habitat
• - Listed by FWS on November 7, 2002 with a recovery priority of 3C.
• 60% cis-5-eicosenoic acid
• 8% cis-5-docosenoic acid
• 10% erucic acid
Photo source: © 2009 Barry Breckling http://calphotos.berkeley.edu/cgi/img_query?enlarge=0000+0000+1109+2575
Limnanthes albaLimnanthes alba• Skin softners for the cosmetic industry
• Biodegradable lubricants
• Inks
• Detergents
• Plasticizers
• Derivatives that can be used as coatings, films, and adhesives
Seasonal wetlands
Annually alters between standing water and extreme drying
Presence of fairy shrimp and other specialized species
Vernal Vernal PoolsPools
Vernal Pool Fairy Vernal Pool Fairy ShrimpShrimp
Branchinecta lynchi Branchinecta lynchi
• Germination Study To determine the effect of 6 presoak
treatments on germination rates
• Population Comparison 4 populations were compared for
reproductive viability by measuring germination rates, flowers per plant and number of seeds per flower
• Inbreeding Depression Study Inbred seed was produced in an insect free
greenhouse and morphological differences in wild collected seed and greenhouse grown inbred seed were observed.
.2% Potassium nitrate
.2% Potassium nitrate and .05% GA
.05% GA
.25% GA
.5% GA
Water
Presoak Treatments for Presoak Treatments for Germination StudyGermination Study
Vleeshouwers, L. M., H. J. Bouwmeester and C. M. Karssen. “Redefining Seed Dormancy: An Attempt to Integrate Physiology and Ecology.” Journal of Ecology, Vol. 83, No. 6 (Dec., 1995), pp. 1031-1037. Published by: British Ecological Society
Mean Percent Germination Mean Percent Germination Rates for Presoak TreatmentsRates for Presoak Treatments
• Largest population of L. floccosa ssp. grandiflora in Oregon
• Non-native species least dense and numerous
• More native grasses surrounding the pools
• No immediate threats from development or recreational activities
• Owned and maintained by Jackson County
• Second largest population of L. floccosa ssp. grandiflora in Oregon
• Largest threat is non-native grasses, although currently native species still dominate the pools
• Owned and maintained by The Nature Conservancy
Invaded by non-native species
Buried gas pipeline created a series of artificial vernal pools
Owned and maintained by Jackson County
• Small population of L. floccosa ssp. grandiflora
• Vernal pools are heavily invaded by buckbrush, which is a native species
• Owned and maintained by The Nature Conservancy
Mean Percent Germination Rates for Mean Percent Germination Rates for Populations of Populations of L. floccosaL. floccosa ssp. ssp. grandifloragrandiflora
A
B
B
B
Mean # of Flowers Per Plant in Mean # of Flowers Per Plant in Each Population Each Population
A A
AB
B
Mean # of Seeds Per Flower in Mean # of Seeds Per Flower in Each Population Each Population
ABA
BB
Population ConclusionsPopulation Conclusions• Jackson County School
- Highest germination rate- Larger plants- More seeds/flower- These trends indicate healthiest population or possibility
of inbreeding depression in other populations
•Agate Desert Preserve- No difference in seeds/flower- No difference in plant size
• Savanna- Lowest germination rate- Larger plants, but with fewer seeds/flower
• Weigh Station- Low germination rate- Smaller plants with fewer seeds/flower
Inbreeding DepressionInbreeding Depression
Why important for Vernal Pool species?
• Small isolated islands aid in decreased gene flow among populations
• May reduce the overall genetic variability in population
• Many vernal pool species currently threatened or endangered due to habitat loss
• Makes the results of severe population reducing events (such as bottlenecks) more extreme
The loss of fitness and/or fecundity due to increased homozygosity
Germinate 200 wild collected seeds from Agate, School, and Weigh Station populations and 150 from Savanna population
Assess breeding system via manual outcrossing
Bag the plants to be inbred at budding stage to ensure self pollination
Collect f1 seed, germinate and grow to seed
Examine morphological differences in plants grown from f1 seed and plants grown from wild seed in each of the 4 populations
Inbreeding Depression StudyInbreeding Depression Study
Inbred Seed Weight vrs Inbred Seed Weight vrs Outcrossed Seed WeightOutcrossed Seed Weight
A
B
Number of Seeds Per FlowerNumber of Seeds Per Flower
A
A
• Oregon Department of Agriculture Native Plant Conservation Program– Dr. Robert Meinke– Dr. Stephen Meyers– Rebecca Currin– Kelly Amsberry
• Howard Hughes Medical Institute
• The Ernest and Pauline Jaworski Fund
AcknowledgemenAcknowledgementsts