Polyurethane-based scaffolds for myocardial tissue engineering by Valeria Chiono, Pamela Mozetic,...

Polyurethane-based scaffolds for myocardial tissue engineering

by Valeria Chiono, Pamela Mozetic, Monica Boffito, Susanna Sartori, Emilia Gioffredi, Antonella Silvestri, Alberto Rainer, Sara Maria Giannitelli, Marcella

Trombetta, Daria Nurzynska, Franca Di Meglio, Clotilde Castaldo, Rita Miraglia, Stefania Montagnani, and Gianluca Ciardelli

Interface FocusVolume 4(1):20130045

February 6, 2014

©2014 by The Royal Society

ATR-FTIR spectrum of PU.

Valeria Chiono et al. Interface Focus 2014;4:20130045


Storage (G′, triangles) and loss (G′′, squares) modulus as a function of temperature (temperature ramp test; heating scan from 80°C to 200°C at 10°C min−1; frequency = 0.5 rad s−1; strain =

0.5%).



G′ (triangles) and G′′ (squares) (a) and complex viscosity (b) as a function of frequency (frequency range = 0.1–100 rad s−1; strain = 0.5%; 165°C).



G′ (triangles) and G′′ (squares) as a function of time (time sweep test; frequency = 0.5 rad s−1; strain = 0.5%; 165°C; 30 min).



DSC thermograms of PU sample.



TGA and DTGA curves of PU. The analysis was carried out under air in the 50–800°C temperature range at a heating rate of 10°C min−1.



Percentage of weight as a function of time during isothermal TGA analysis at 165°C, for 1 h, under air.



(a) FEG-SEM micrograph of a PU scaffold obtained by melt-extrusion AM; (b) higher magnification detail of the trabecular arrangement.



Stress–strain behaviour during uniaxial tensile test (load cell: 10 N; strain rate: 0.8 min−1).



Optical and confocal micrographs of PU scaffold seeded with CPCs for 3 days: (a) phase contrast microscopy, scale bar 200 µm; (b–f) confocal microscopy merged images (magnification

10×) of Ki67 (b, green), actin (c,d, green), vimentin (e,f, green) and cell ...



SEM micrographs of PU scaffolds cultured with human CPCs for (a) 7 and (b) 14 days.



Proliferation of human CPCs in contact with PU scaffolds for 1, 4, 7 and 14 days assessed by measuring total DNA content.



Polyurethane-based scaffolds for myocardial tissue engineering by Valeria Chiono, Pamela Mozetic,...

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