Polyclonal ANTIBODY Adiponectin - ProteintechBackground Information Adiponectin (AdipoQ), an...
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Adiponectin Polyclonal ANTIBODY Catalog Number:21613-1-AP 2 Publications For Research Use Only www.ptglab.com Basic Information Catalog Number: 21613-1-AP Size: 60 μg/150 μl Source: Rabbit Isotype: IgG Purif ication Method: Antigen af f inity purif ication Immunogen Catalog Number: AG16304 GenBank Accession Number: BC096308 GeneID (NCBI): 9370 Full Name: adiponectin, C1Q and collagen domain containing Calculated MW: 244aa,26 kDa Observed MW: 29 kDa Recommended Dilutions: WB 1:200-1:1000 IHC 1:50-1:500 IF 1:50-1:500 Applications Tested Applications: IF, IHC, WB, ELISA Cited Applications: WB Species Specif icity: human, mouse Cited Species: human Note: suggested angen retrieval with TE buffer pH 9.0; (*) Alternavely, angen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : NIH/3T3 cells; 3T3-L1 cells IHC : human liver tissue; human skin tissue IF : NIH/3T3 cells; Background Information Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving insulin sensitivity and anti-inf lammatory activity. The role of AdipoQ in reproduction is not yet f ully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its ef f ects by activating a range of dif f erent signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth f actors. Notable Publications Author Pubmed ID Journal Application Jinghui Jiang 30745818 Int J Biol Sci WB Xuerong Sun 29288588 Cell Biol Int WB Storage Storage: Store at -20ºC. Stable f or one year af ter shipment. Storage Buf f er: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary f or -20 º C storage T: 1 (888) 4PTGLAB (1-888-478-4522) (toll f ree in USA), or 1(312) 455-8498 (outside USA) E: [email protected] W: ptglab.com For technical support and original validation data f or this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of Polyclonal ANTIBODY Adiponectin - ProteintechBackground Information Adiponectin (AdipoQ), an...
AdiponectinPolyclonal ANTIBODYCatalog Number:21613-1-AP 2 Publications
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:21613-1-APSize:60 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG16304
GenBank Accession Number:BC096308GeneID (NCBI):9370Full Name:adiponectin, C1Q and collagen domaincontainingCalculated MW:244aa,26 kDaObserved MW:29 kDa
Recommended Dilutions:WB 1:200-1:1000 IHC 1:50-1:500 IF 1:50-1:500
Applications Tested Applications:IF, IHC, WB, ELISACited Applications:WBSpecies Specif icity:human, mouseCited Species:humanNote: suggested an gen retrieval with TEbuffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0
Positive Controls:WB : NIH/3T3 cells; 3T3-L1 cellsIHC : human liver tissue; human skin tissueIF : NIH/3T3 cells;
Background Information Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number ofmetabolic processes, including improving insulin sensitivity and anti-inf lammatory activity. The role of AdipoQ in reproduction is not yet fully understood, butthe expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and humanplacenta. Adiponectin exerts its ef fects by activating a range of dif ferent signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cellgrowth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.
Notable Publications Author Pubmed ID Journal Application
Jinghui Jiang 30745818 Int J Biol Sci WB
Xuerong Sun 29288588 Cell Biol Int WB
Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage
T: 1 (888) 4PTGLAB (1-888-478-4522) (tollf ree in USA), or 1(312) 455-8498 (outsideUSA)
E: [email protected] W: ptglab.com
For technical support and original validation data for thisproduct please contact:
This product is exclusively available underProteintech Group brand and is not available topurchase from any other manufacturer.
Selected Validation Data
Immunohistochemistry of paraff in-embeddedhuman liver tissue slide using 21613-1-AP(ADIPOQ antibody) at dilution of 1:200 (under 10xlens).
Immunohistochemistry of paraff in-embeddedhuman liver tissue slide using 21613-1-AP(ADIPOQ antibody) at dilution of 1:200 (under 40xlens).
NIH/3T3 cells were subjected to SDS PAGEfollowed by western blot with 21613-1-AP(ADIPOQ Antibody) at dilution of 1:300 incubatedat room temperature for 1.5 hours
Immunofluorescent analysis of ( 4% PFA ) f ixedNIH/3T3 cells using 21613-1-AP(ADIPOQantibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Aff iniPure Goat Anti-Rabbit IgG(H+L)
