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CHAPTER 4

\ACTIVITY 4,2 Plant transport systems

Prelab activity (key concepts)

Tracheid

"::rr - ::: .3, vCE Units 7&2 Student Activv Manual A 2006 Nelson Australia Pty Ltd

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lntrod uction\Vater is an essential requiremenr for photos'nthesis ro occur. As photosynthesis mostly occurs in theIeaves of plants, warer and subsrances dissohled in it, move ,h.o.rgi vascular plants in the xylem, fromthe roots to the lea'es' Xriem consists ofhollorv cells oftracheids and vessels. Sugars, in the form ofthedisaccharide sucrose' and other nurrients move in the phloem. The phloem is made up of sieve cells andcompanion cells.

Pu rposeTo examine ho*'*'arer is transported through a celery stalk and leaves.

MaterialsEach group rvill require:

fJ a srick ofcelery that has been standing in red food dye or eosin for several hours, cut2 cmfrom itsbase

[l single-edged razor bladefl microscope, srereo microscope or hand iensfl microscope slides

fl mounted needles (optional)fl millimetre ruler' transverse and longitudinal stem se-ions of Helianthu.r (for Extension Activities).

Procedurefl R.rnou" rhe celery from the coloured solution.U Examine the stalk and leaves for spread of the dye; holding them up to the light may help. observethe areas where the dye is concentrated. Draw the distribuiion of die in ,n. rlri" eigure 4.2 under'Observations' below.

fl cut thin (t mm) transverse sections across the stem and branch (and leaf if possible). Arrange thesections onto a microscope slide.D E*amine the sections ,,,,d.. loru po\\'er and make diagrams shorving the distribution of the dye.D C.,t anorher fresh 1-2 mm piece of stem.fl utt the razor blade to cut out a small piece of rhe area coloured by the dye and put this on amicroscope slide.

D ur. a couple of razor brades or mounted needles ro rease the piece of tissue apart.E aaa a coverslip, using a drop of water if the tissue is becoming dry.c ur. low power, th.., high po-., to find 'spirai' or 'coils,. Drari, th"se in Figure 4.3.

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OhservationsFigure 4.2 Distribution of dye in celery leaf.

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Figure 4.3 Spirals/coils found in vascular tissue of celery.

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c The spirals have functions equivalent to those of the spirals of cartilage around the trachea.\7hat are their functions?

ConclusionRefer back to the purpose of this activin'- Have you achieved this purpose? \Write a conclusion based onwhat you have learnt by undertaking rhis activiry.

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