welcomewelcome

SREE NARAYANA TRAINING SREE NARAYANA TRAINING COLLEGECOLLEGE POOCHAKKAL POOCHAKKAL

zaza

SSUBMITTED BY UBMITTED BY GEETHUMOL K.K GEETHUMOL K.K NATURAL SCIENCE NATURAL SCIENCE REG NO: 13383005 REG NO: 13383005SUBMITTED TOSUBMITTED TO SOYA .P SOYA .P LECTURE IN NATURAL LECTURE IN NATURAL SCIENCESCIENCE

aa

POWER POINT PRESENTATION ON POWER POINT PRESENTATION ON PLANT TISSUE CULTURE PLANT TISSUE CULTURE

aa

W h at i s Pl an t Ti ssu e Cu l t u r e?W h at i s Pl an t Ti ssu e Cu l t u r e?

Plant t issue cult ur e is a f or m of Plant t issue cult ur e is a f or m of asexual pr opagat ion of plant s under asexual pr opagat ion of plant s under labor at or y condit ions.labor at or y condit ions.

W h y i s Pl an t Ti ssu e Cu l t u r e I mpo r t an t ?W h y i s Pl an t Ti ssu e Cu l t u r e I mpo r t an t ?

I t has made signif icant cont r ibut ions I t has made signif icant cont r ibut ions in:in: The pr oduct ion of plant mat er ialThe pr oduct ion of plant mat er ial Plant br eedingPlant br eeding Gene banksGene banks The pr oduct ion of chemical compoundsThe pr oduct ion of chemical compounds

H o w i s Pl an t Ti ssu e Cu l t u r e D o n e?H o w i s Pl an t Ti ssu e Cu l t u r e D o n e?

I t is gr own on a special cult ur e I t is gr own on a special cult ur e medium which suppor t s it s gr owt h and medium which suppor t s it s gr owt h and development .development .

The medium can be eit her semisolid, The medium can be eit her semisolid, such as agar , or liquid, such as such as agar , or liquid, such as pur if ied wat er .pur if ied wat er .

M i c r o o r g an i sms an d Pl an t Ti ssu e M i c r o o r g an i sms an d Pl an t Ti ssu e Cu l t u r eCu l t u r e

I n t he pr act ice of plant t issue cult ur e, I n t he pr act ice of plant t issue cult ur e, micr oor ganisms ar e called “cont aminant s” micr oor ganisms ar e called “cont aminant s” because of t heir har mf ul ef f ect s on plant because of t heir har mf ul ef f ect s on plant gr owt h in vit r o.gr owt h in vit r o.

Six pot ent ial sour ces of cont aminat ion in Six pot ent ial sour ces of cont aminat ion in t he plant t issue cult ur e lab ar e:t he plant t issue cult ur e lab ar e: AirAir Wat erWat er Gr owt h MediaGr owt h Media PeoplePeople EquipmentEquipment Plant Mat er ialPlant Mat er ial

Culturing (micropropagating) Culturing (micropropagating) Plant Tissue - the stepsPlant Tissue - the steps

Selection of the plant Selection of the plant tissuetissue ( (explantexplant) from a ) from a healthy vigorous healthy vigorous ‘mother plant’ - this is ‘mother plant’ - this is often the apical bud, often the apical bud, but can be other tissuebut can be other tissue

This tissue must be This tissue must be sterilized to remove sterilized to remove microbial contaminantsmicrobial contaminants

The Steps, IIThe Steps, II EstablishmentEstablishment of the of the

explant in a culture explant in a culture medium. The medium medium. The medium sustains the plant cells sustains the plant cells and encourages cell and encourages cell division. It can be solid division. It can be solid or liquidor liquid

Each plant speciesEach plant species (and (and sometimes the variety sometimes the variety within a species) has within a species) has particular medium particular medium requirements that must requirements that must be established by trial be established by trial and errorand error

The Steps, IIIThe Steps, III

MultiplicationMultiplication- The - The explant gives rise to a explant gives rise to a calluscallus (a mass of (a mass of loosely arranged cells) loosely arranged cells) which is manipulated by which is manipulated by varying sugar varying sugar concentrations and the concentrations and the auxinauxin ( (lowlow): ): cytokinincytokinin ((highhigh) ratios to form ) ratios to form multiple shootsmultiple shoots

The callus may be The callus may be subdivided a number of subdivided a number of timestimes

Dividing shoots

Warmth and good light are essential

The Steps, IVThe Steps, IV

Root formationRoot formation - The - The shoots are shoots are transferred to a transferred to a growth medium with growth medium with relatively relatively higherhigher auxin: cytokinin auxin: cytokinin ratiosratios

The bottles on these racks are young banana plants and aregrowing roots

Tissue culture plants sold toa nursery & then potted up

The Steps, VThe Steps, V

The rooted shoots are The rooted shoots are potted up (deflasked) potted up (deflasked) and ‘hardened off’ by and ‘hardened off’ by gradually decreasing gradually decreasing the humiditythe humidity

This is necessary as This is necessary as many young tissue many young tissue culture plants have no culture plants have no waxy cuticle to prevent waxy cuticle to prevent water losswater loss

THANK YOU