Plant Diseases Forensic Diagnostic Methods Andres S. Espindola Dept. Entomology and Plant Pathology...
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Transcript of Plant Diseases Forensic Diagnostic Methods Andres S. Espindola Dept. Entomology and Plant Pathology...
Plant DiseasesForensic Diagnostic Methods
Andres S. EspindolaDept. Entomology and Plant Pathology
Oklahoma State University
Plant Diagnostics: Importance
Plant diseases spread as quick as human
diseases
Infected plants can not be saved.
Quick plant pathogen diagnosisQuick on site diagnosisSpecialized lab
The United States crops are a billionaire business
Signs and SymptomsAt a glance, take a look in signs and symptoms.
Pseudomonas syringae pv. tomato
Signs: Presence of the pathogen (bacterial cells)
Signs and symptoms may give a guidance to plant pathologist.
Symptoms: responses of the plant.
Different techniques for Diagnosis
Symptoms and signs are not the only tool to
diagnostic a plant disease.
DNA RNA Proteins
Molecular Techniques:Polymerase Chain
Reaction (PCR)
Immunological tests:Immunostrips
Agglutination tests
Immunological tests:Immunostrips
Agglutination tests
Immunological diagnostic tests
Animal Antibodies
Plant Pathogen Protein
Human Immune System
Plant pathogen proteins injection
Immunostrip TestPositive Result
Negative Result Invalid Results
Pictures of real Immunostrips
Agglutination
The results are going to be similar to this:
AntibodiesBacteria (Plant pathogen)
Agglutination: How does it happen?
Agglutination: How does it happen?
Thank youThank you