Pioneering Mars: Turning the Red Planet Green
description
Transcript of Pioneering Mars: Turning the Red Planet Green
![Page 1: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/1.jpg)
Growing Cyanobacteria
in the Lab
Scott P. Milroy, Ph.D.USM Department of Marine Science
14-19 November 2012
Pioneering Mars:Turning the Red Planet Green
BAKERST STAN
![Page 2: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/2.jpg)
Pioneering Mars The Basics of Algal Culture
Several growth chambers (flasks) are prepared with liquid growth media, which contains:
Ultrapure Water
![Page 3: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/3.jpg)
Pioneering Mars The Basics of Algal Culture
Several growth chambers (flasks) are prepared with liquid growth media, which contains:
Ultrapure WaterSea Salts
![Page 4: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/4.jpg)
Pioneering Mars The Basics of Algal Culture
Several growth chambers (flasks) are prepared with liquid growth media, which contains:
VitaminsSea Salts
![Page 5: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/5.jpg)
Pioneering Mars The Basics of Algal Culture
Several growth chambers (flasks) are prepared with liquid growth media, which contains:
VitaminsMinerals
![Page 6: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/6.jpg)
Pioneering Mars The Basics of Algal Culture
Several growth chambers (flasks) are prepared with liquid growth media, which contains:
NutrientsMinerals
![Page 7: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/7.jpg)
Several growth chambers (flasks) are prepared with liquid growth media, which contains:
Pioneering Mars The Basics of Algal Culture
The flasks and growth media must then be sterilized to prevent bacterial or fungal contamination (i.e. “axenic” cultures)…
Nutrients
![Page 8: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/8.jpg)
The flasks and growth media must then be sterilized to prevent contamination…
The high pressure and steam within the autoclave is an effective way to sterilize delicate equipment, flasks, and growth media.
Pioneering Mars The Basics of Algal Culture
![Page 9: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/9.jpg)
The high pressure and steam within the autoclave is an effective way to sterilize delicate equipment, flasks, and growth media.
Pioneering Mars The Basics of Algal Culture
The sterile growth media can now be inoculated with a small volume from the “parent” culture
![Page 10: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/10.jpg)
The sterile growth media can now be inoculated with a small volume from the “parent” culture
Pioneering Mars The Basics of Algal Culture
![Page 11: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/11.jpg)
The sterile growth media can now be inoculated with a small volume from the “parent” culture
Pioneering Mars The Basics of Algal Culture
The “daughter” cultures are then placed in a light- and temperature-controlled incubator and allowed to grow…
![Page 12: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/12.jpg)
Pioneering Mars The Basics of Algal Culture
Algal cultures are typically run either as a BATCH culture or as a CONTINUOUS culture…
BATCH CONTINUOUS
![Page 13: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/13.jpg)
Pioneering Mars The Basics of Algal Culture
Algal cultures are typically run either as a BATCH culture or as a CONTINUOUS culture…
BATCH CONTINUOUS
Because we need small, portable, sealed growth chambers, it makes the most sense for us to use BATCH cultures for our experiments…
![Page 14: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/14.jpg)
Because we need small, portable, sealed growth chambers, it makes the most sense for us to use BATCH cultures for our experiments…
During our workshop, we’ll determine the fundamental parameters of our culture experiments, such as:
Pioneering Mars Things to Think About
BATCH◊ Type & Concentration of Dissolved Salts? (Best representation of wet Martian soils)
![Page 15: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/15.jpg)
During our workshop, we’ll determine the fundamental parameters of our culture experiments, such as:
Pioneering Mars Things to Think About
◊ Type & Concentration of Dissolved Salts? (Best representation of wet Martian soils)
◊ Vitamins, Minerals, and/or Nutrients? (Ideal culture conditions vs Martian reality)
![Page 16: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/16.jpg)
During our workshop, we’ll determine the fundamental parameters of our culture experiments, such as:
Pioneering Mars Things to Think About
◊ Type & Concentration of Dissolved Salts? (Best representation of wet Martian soils)
◊ Vitamins, Minerals, and/or Nutrients? (Ideal culture conditions vs Martian reality)
◊ UV/Visible Light Quality & Quantity? (Irradiance vs Photoperiod on Martian surface)
![Page 17: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/17.jpg)
During our workshop, we’ll determine the fundamental parameters of our culture experiments, such as:
Pioneering Mars Things to Think About
◊ Type & Concentration of Dissolved Salts? (Best representation of wet Martian soils)
◊ Vitamins, Minerals, and/or Nutrients? (Ideal culture conditions vs Martian reality)
◊ UV/Visible Light Quality & Quantity? (Irradiance vs Photoperiod on Martian surface)
◊ Pressure and Temperature Consequences? (Freeze/Thaw Survival & H2O Triple-point)
![Page 18: Pioneering Mars: Turning the Red Planet Green](https://reader036.fdocuments.net/reader036/viewer/2022081511/5681663f550346895dd9a8fb/html5/thumbnails/18.jpg)
During our workshop, we’ll determine the fundamental parameters of our culture experiments, such as:
Pioneering Mars Things to Think About
◊ Type & Concentration of Dissolved Salts? (Best representation of wet Martian soils)
◊ Vitamins, Minerals, and/or Nutrients? (Ideal culture conditions vs Martian reality)
◊ UV/Visible Light Quality & Quantity? (Irradiance vs Photoperiod on Martian surface)
◊ Cyanobacteria vs Other Extremeophiles? (Perhaps there are other options - rhodophytes?)
◊ Pressure and Temperature Consequences? (Freeze/Thaw Survival & H2O Triple-point)