Photothermal Antibacterial and Antibiofilm Activity of ...
Transcript of Photothermal Antibacterial and Antibiofilm Activity of ...
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Supporting Information
Photothermal Antibacterial and Antibiofilm
Activity of Black Phosphorus/Gold
Nanocomposites against a Pathogen Bacteria
İlknur Aksoya, Hüseyin Küçükkeçecib, Fatih Sevgic, Önder Metinb,*, Imren Hatay Patira,*
a Department of Biotechnology, Selcuk University, 42031, Konya, Turkey.
b Department of Chemistry, College of Sciences, Koç University, 34450, Istanbul, Turkey.
c Vocational School of Health Services, Department of Medical Services and Techniques,
Selcuk University, 42031, Konya, Turkey.
e-mail: [email protected]; [email protected]
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Figure S1. Raman spectra of as-synthesised BP crystals.
Figure S2. (a) Time-resolved emission spectra of bare BP and BP/Au nanocomposites and (b) a table showing the
lifetimes (τi) and amplitudes (Ai) of the lifetime decays of BP and BP/Au nanocomposites.
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Figure S3. Concentration (µg ml-1) of BP nanosheets (a) and BP/Au nanocomposites (b) dependent absorbance
graphs. (a) a: bacteria, b: NIR light, c: BP (32 µg ml-1) + NIR light, d: BP (64 µg ml-1) + NIR light, e: BP (128 µg ml-1) + NIR light. (b) a: bacteria, b: NIR light, c: BP/Au (32 µg ml-1) + NIR light, d: BP/Au (64 µg ml-1) +
NIR light, e: BP/Au (128 µg ml-1) + NIR light.
Figure S4. SEM images of (a) the untreated E. faecalis (blank) and (b) after the treatment of BP/Au
nanocomposites (128 µg ml-1) under NIR light irradiation. Scale bars are 200 nm.
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Figure S5. GSH depletion after different treatments at the concentration of 128 µg ml-1. The loss of GSH levels
(%) of H2O2, BP, BP/Au, BP/NIR and BP/Au/NIR are 79, 11, 19, 27 and 28, respectively.
Figure S6. Standard curve of GSH. The absorbance of each solution was measured at 405 nm. Residual GSH
concentration levels were measured according to the equation y= 0,0062x – 0,0233. X axis is concentration, y
axis is absorbance.
y = 0.0062x - 0.0233R² = 0.9987
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0 10 20 30 40 50 60 70 80 90 100
Ab
sorb
ance
Concentration (µM )
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Table S1. A table showing the previous studies about antibiofilm activity of 2D nanomaterials against various
microorganisms including E. faecalis with the biofilm inhibition percentage and suggested inhibition mechanisms.
Nanomaterial Concentrati
on (µg/ml)
Bacteria
tested
Biofilm
inhibition/eradication
(%)
Suggested mechanism Ref
GO
nanosheets
100 E. coli and P.
aeruginosa
~50% Cell membrane disruption 1
Graphene
nanoplatelets
- S. mutans 56% Mechanical damage of
cell wall
2
GO, GO-Tob
and GO-
Tob@CuS
100 S. aureus and
P. aeruginosa
20%, 50% and 80% Nano-knife, temperature
increase and ROS.
3
g-C3N4 - S. epidermidis - ROS generation 4
Ag/g-C3N4 200 S. aureus 70% ROS generation 5
MoS2
nanosheets
30 MRSA ~70% ROS-independent
oxidative stress and
membrane depolarization
6
TiO2 and Ag-
TiO2
composite
- E. coli and S.
aureus
56% and 77% for E.
coli
43% and 67% for S.
aureus
Disruption of membrane
integrity and
photocatalytic activity
7
NanoZnO/Ze 10.000 E. faecalis 85,2% Repression of a gene
associated with biofilm
formation
8
Au
nanoparticles
20 C. albicans < 1% Photodynamic therapy 9
BP/Au
nanocomposi
tes
64 E. faecalis 58 % Photothermal effect,
oxidative stress and
physical membrane
damage
This
work
References
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