Photo by Alyson Bahr

Photo by Alyson Bahr

Oceanographic Sampling:Oceanographic Sampling:

Plankton TowPlankton Tow

Kahoali’i Keahi


Aiea High School students aboard the Honu Kai

Plankton Tow Video from Big Rapa


Plankton Tow SafetyPlankton Tow Safety• Sampling near/on/in water: KNOW YOUR ABILITY

•When in doubt, get out

• Be Mindful of ocean conditions

• Never attach the net to yourself

• Never wrap line around hands, arms, body, etc.

• Watch for rocks, coral, and other stuff in water• Oceanographic research ship mantra: Anything you put over the side and actually want to get back – tie it to the boat!



Where to sample:• From shore (dock, bridge, etc.)• In the water (wading, swimming)• On the water (kayak, canoe, surfboard, SUP, boat)

Where to sample:• From shore (dock, bridge, etc)

• Wear safety gear• Watch for obstacles in water•Let drift in current, walk and drag net, or vertical up-downs• Be mindful of the net• Be mindful of conditions• Tie net to shore



Where to sample:• In the water (wading, swimming)

• Hazardous Do Not Attempt• Location important

(i.e. DO NOT sample Sandy’s)• Get past shore break•Watch for rocks, coral • Net adds a LOT of drag • Know your ability!!!



Cory Lum for The New York Times

www.britannica.com



Where to sample:• On the water (kayak, canoe, surfboard, SUP, boat)

• Hazardous Do Not Attempt• Always have net attached to boat• Tow slow < 1 knot• Lots of drag, could pull you in• Watch for rocks, coral, propeller• Always ask boat captain first!


Plankton TowPlankton TowWhat to bring:• Net• Line, long or short• Sample bottle• Rinse bottle• Sample Jar• Bucket• Timer• Bottle for seawater

Remember the mantra?


Plankton TowPlankton TowAttaching line to net: The bowline



Step 1:

1. Make a loop near the end of the line



Step 2:

2. Pull the end through the loop



Step 3:

3. Tuck the end under the main line



Step 4:

4. Pull the end back through the loop in the main line



Step 5:

5. Pull the end and main line in opposite directions to tighten



Now retie so that the tow ring on the net is in the loop!

Attaching line to net:


Plankton TowPlankton TowSampling procedures:1.Double check that equipment is ready and safe to deploy2.Carefully ease the net into the water, start the clock3.Record any relevant information on a data sheet if you are planning analytical study4.Walk, wade, swim, paddle, or throttle so that water is flowing through mouth of net5.Tow for 15-30 minutes, be mindful of conditions6.Periodically check sample7.When sampling complete, carefully prepare your sample for transport8.Clean up

Rinse net thoroughly with fresh water and allow to dry before storing

Next Step: Viewing PlanktonNext Step: Viewing Plankton

MicroscopyMicroscopy

Setting up the microscope:Setting up the microscope:

Parts Included:

Calibration slideFrosted Stage plate

Dust Cover, light filter, C-wrench

Eyecups

Cables Included:

Power:

USB

Where to hold when picking up:


• Check the packing list to make sure that everything is included• Carefully remove all of the plastic coverings• Attach the camera cable to the vertical pole (Only goes one way!)• Turn to page 21 and 22 and familiarize yourself with the parts of the microscope


• Check the packing list to make sure that everything is included• Carefully remove all of the plastic coverings• Attach the camera cable to the vertical pole (Only goes one way!)• Turn to page 21 and 22 and familiarize yourself with the parts of the microscope• Plug in and focus the microscope (p. 23)

Setting up the microscope and Setting up the microscope and computer:computer:

• Install Motic Images Plus Software DO NOT USE MOTIC CD!• Restart computer• Move help files to help folder• Connect microscope and computer via USB cable• Wait until driver installs • Open Motic images plus and try to get image on screen (p. 24)

Setting up the microscope and Setting up the microscope and computer:computer:

• If you plan to record video, install the Xvid codec• If you plan to use the measurement feature, calibrate the microscope (25)• Else view plankton and try to identify

If you need to connect to wireless:Username: cmore.education@gmaiPassword: Cmore@1203

Lesson Ideas:Lesson Ideas:

• Identify and draw at least 6 different organisms (try now) • Create your own plankton guide• Conduct plankton studies: (science fair projects)

• Are plankton same different times of day?• Does plankton community change after event (rain, big wind, spill, etc)?

• Add math to study• Calculate the volume of water sampled during a tow• V = open area of net x distance towed • distance towed = speed x time

Lesson Ideas:Lesson Ideas:

• Install Motic software on multiple computers and have students work with saved images• Use measurement tool to make scale drawings or models of plankton• Have a picture of the week

• zoom in on object and have student make hypothesizes based on observations.

• Have students bring in objects to look at and share (as long as related to lessons). • Creature features

End of the Workshop, End of the Workshop,

but not the end of training:but not the end of training:

• Share the resources with other teachers at school• Follow up webinars for additional training

• can schedule for department meetings• Technical support• Hawaii plankton network?• Be one of our STARS

End of the Workshop, End of the Workshop,

but not the end of training:but not the end of training:

• Evaluation – Help us improve for the next workshop & expect a follow up evaluation sometime next year

Photo by Alyson Bahr

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