Phat Trien Vsv

8/9/2019 Phat Trien Vsv

1/36

Ph t trin vi sinh vt lin quan

t i tng s tbo, khng lin quant i l n ca tboY

Cc i hi cho s ph t trinVt lHo hc


2/36

C c i hi vt l Y N hit

- a lnh (psychrophiles) (cold loving microbes )Di nhit 0 C - 20 C

- a m (mesophiles) (moderate temp. lovingmicrobes)

Di nhit 20 C - 40 C

Chi nhit (thermophiles) (heat lovingmicrobes)Di nhi t 40 C - 100 C


3/36

pH Y Hu ht vi sinh vt ph t trin trong pH 6.5 - pH

7.5

Y Rt t c thpht trin d- i pH 4.0N hiu thc phm, nh- sauerkraut, d- a mui, v pho mt- c bo qun khi s h- hng do acid - c to ratrong qu trnh ln men


4/36

p sut thm thu Y Hu ht vi sinh vt nhn - c tt c cc cht

dinh d- ng trong dung dch t mi tr- ngn- c xung quanh

Y Tnh c- ng (Tonicity)Tnh c- ng va (isotonic)

Tnh c- ng nhiu (hypertonic)Tnh c- ng t (hypotonic)


5/36

Cells


6/36

C c i hi ho hc Y Macro & Micro E lements

Y C HOPKINS CaFe Mg Na Cl


7/36

OxygenY Vi sinh vt cn phi - c phn lo i da trn

cc i hi oxygen


8/36

1. Vi sinh vt hiu kh(obligateAerobe)


9/36

2. Vi sinh vt k kh(obligateAnaerobe)


10/36

3 . Vi sinh vt hiu khkhng btbuc & k khkhng bt buc(Facultative Aerobe &FacultativeAnaerobes)


11/36

4. Vi sinh vt cn rt t kh(Microaerophilic)


12/36

Oxy gen lm cht mt s vi sinhvt Y Tt c vi sinh vt sinh ra superoxide ( O2-)

Y Superoxide lad c cho tbo(ly i c c int)

Y Superoxide cn phi - c trung ho


13/36

Su peroxide dism uta se

Y O2- + O

2- + 2 H+ -------> H

2O

2+ O

2

Y Hydrogen peroxide cng l c t cho tbo v cn phi trung ho n


14/36

C atala se

Y 2 H2O

2 --------> 2 H

2O + O

2

Y Vi sinh vt hiu khbt buc khuyt:Superoxide dismutase ( SOD )Catalase


15/36

M i tr- ng nui c y Y 1. Thnh phn ho hc xc nh

Bit - c chnh xc thnh phn ho hcS dng ph t trin cc vi sinh vt kh tnh

Y 2. Mi tr- ng hn h p (Complex Media)Khng bit chnh x c thnh phn ho hcHu ht vi khun v nm mc ph t trin - ctrn mi tr- ng ny


16/36

C c k thut nui c y c bit Y 1. Vi khun k kh(Anaerobic Bacteria)

a. Gim b t mi tr- ng (Reducing Media)b. Bung k kh(Anaerobic Container)c. Agar Stabd. Agar Shake


17/36

C c k thut nui c y c bit Y 2. Vi khun t hiu kh(Microaerophilic

Bacteria)Pht trin tt nht O2 gim b t v tng mc

Khng khbnh th- ng 21 % O2

.3 n .03 % CO2


18/36

Vi khun t khkh(Microaerophilic Bacteria)A. Bnh nn (Candle Jar)

16 % O 2

4 % CO2


19/36

Vi khun it hiu kh

(Microaerophilic Bacteria)B . CO2 Gene rating Pack et


20/36

M i tr- ng chn lc c chs ph t trin ca mt s vi khun trong

khi l i chn lc s pht trin cho cc vikhun khc

Y Vd:Brilliant Green Agar

Mu c chs pht trin vi khun Gram (+)Chn lc cho vi khun Gram (-)Hu ht cc nhim khun - ng rut gy ra do vikhun Gram (-)


21/36

M i tr- ng chn lc Y E MB (E osin Methylene Blue)

Mu c chvi khun Gram (+)Chn lc cho vi khun Gram (-)

Hu ht cc nhim khun - ng rut gy rado vi khun Gram (-)


22/36

M i tr- ng kh c bit Y S khc bit gia cc sinh vt khc nhau

pht trin trn c ng mt aY Vd:

a thch mu (TSA c 5% mu cu)S dng s khc nhau phn bit cc kiu

S

treptococci


23/36

A lph a Hemolyt ic St re pt ococci

Incomplete lys is of R B Cs


24/36

B eta H emolyt ic St re pt ococci

Complete lys is of R B Cs


25/36


26/36

M i tr- ng chn lc v kh c bit Y Mannitol Salt Agar

S dng phn bitS taphy lococcus aureusY Mannitol Salt Agar

N ng mui cao (7.5%) c chhu ht cc vi khun - ng MannitolChthpH (Chuyn mu vng khi acid)


27/36

M i tr- ng chn lc v kh c bit Y MacConkey s Agar

S dng phn bitS almonellaY MacConkey s Agar

Bile salts v crystal violet (c chvi khunGram (+))lactoseChthpH

Ma ny Gram (-) ente ric non- pathogenic bac teria ca n f er ment lactose, Sal mone lla can not


28/36

Ph t trin vi sinh vt t ng s l- ng tbo

Y S sinh sn nhn i (Binary Fission)

Y Thi gian t i to (Doubling Time)thi gian i hi cho tbo nhn iHu ht trn 1 gi n3 gi.

E. coli - 20 minutesM y cobacterium tuberculosis- 24 gi.


29/36

S sinh sn nhn i - unchecked Y E . coli thi gian ti to l 20 min.Y 20 thh(khong 7 gi.)

1 triu tboY 3 0 thh(khong 10 gi.)

1 t tbo

Y 72 thh(khong 24 gi.)1 x 10211,000,000,000,000,000,000,000 tbo


30/36

C c y u t h n chtrong mi tr- ng Y Thiu thc n, n- c hoc cht dinh d- ngY Khng gianY tch t cc cht thi trao i chtY Thiu oxygenY Thay i pHY N hit


31/36

C c pha ph t trinY 4 Pha

Y 1. PhaLagY 2. Pha LogY 3 . PhaStationaryY 4. PhaDeath


32/36

1. Ph a La g Y Vi khun u tin - c - a vo mi tr- ng

ni cyY Vi khun checking out mi tr- ng xungquanhY Cc tbo hot ng trao i cht rt tch

ccY S l- ng tbo thay i rt tY 1 gi n vi ngy


33/36

2 . Ph a L og Y Tbo pht trin nhanh (exponential

growth)Y Qun thtng gp i sau mi thhY Vi sinh vt rt nhy cm v i cc iu kin

bt l iantibioticsanti-microbial agents


34/36

3 . Ph a Stat ion a ryY T lcht = t lsinh snY Cc tbo bt u chm trn v i cc iu

kin bt l i ca mi tr- ngThiu cht dinh d- ngThiu n cKhng khng gianCc cht thi trao i chtoxygenpH

Bt u hnh thnhE ndospore


35/36

4 .Ph

aDeath

Y T lcht > t lt i toY Do cc yu t hn chtrong mi tr- ng


36/36

m vi khunY c mi tr- ng - 10 triu tbo vi khun trn

ml

Y Pha lo ng hng lo t (Serial Dilution)

Phat Trien Vsv

Documents

Transcript of Phat Trien Vsv