Phase Contrast Images Contrast Microscopynsmn1.uh.edu/mrea/OMNB 08/OMNB 08 Lec 4Web.ppt.pdf ·...

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1 Contrast Microscopy Koehler Illumination Dark Field Phase Contrast Differential Interference Contrast Hoffman Modulation Phase Contrast Images Brightfield Phase Contrast Phase Contrast Microscopy F. Zernike, 1930s Phase Contrast Microscopy

Transcript of Phase Contrast Images Contrast Microscopynsmn1.uh.edu/mrea/OMNB 08/OMNB 08 Lec 4Web.ppt.pdf ·...

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Contrast Microscopy

Koehler Illumination

Dark Field

Phase Contrast

Differential Interference Contrast

Hoffman Modulation

Phase Contrast Images

Brightfield Phase Contrast

Phase Contrast MicroscopyF. Zernike, 1930s

Phase Contrast Microscopy

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Phase Contrast Images

Brightfield Phase Contrast

Contrast Microscopy

Koehler Illumination

Dark Field

Phase Contrast

Differential Interference Contrast

Hoffman Modulation

DIC (Nomarski) Images

Phase Contrast Nomarski Optics

James Maxwell(1831-1879)

Electromagnetism

ElectromagneticTheory of Light

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Wave Nature of Light

Under some conditions, light behaves as an electromagnetic wave(Maxwell, 1855). In this model, electric and magnetic fields oscillateperpendicular to one another and to the direction of propagation of thelight wave.

Refractive Polarization

David Brewster(1781 - 1868)

Brewster Angle[53o - 56o]

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Optical Birefringence

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Differential Interference ContrastMicroscopy

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DIC

PhaseContrast

PhaseContrast

DIC

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Hoffman Modulation

Hoffman Modulation Images

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Phase Contrast sensitive Imaging Dr M A Oghabian.

Phase Contrast sensitive Imaging Dr M A Oghabian.

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CT abdomen 1. بسم الله الرحمن الرحيم CT Abdomen Axial Oral contrast Without I.V. contrast& With I.V. contrast Arterial phase & Venous phase Delayed images.

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nd 2 Workshop on MRI Phase Contrast & Quantitative …pre.weill.cornell.edu/mri/QSM13/pages/2nd Annual MRI Phase Contra… · MRI Phase Contrast & Quantitative Susceptibility Mapping

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Ion Selective Dyesnsmn1.uh.edu/mrea/OMNB 08/OMNB 08 Lec 9Web.ppt.pdfGreen Fluorescent Protein The excitation spectrum of native GFP from A. victoria (blue) has two excitation maxima

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phase contrast microscope

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Simultaneous Atomic Force and Phase Contrast Microscopy ... · PDF fileSimultaneous AFM/ Phase Contrast Optical phase contrast microscopy is a popular technique for imaging low contrast,

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1.2 Phase contrast imaging - hu-berlin.decrysta.physik.hu-berlin.de/~kirmse/pdf/2012_VL... · Tomography . Phase contrast imaging (high resolution TEM: HRTEM) Phase shift due to the

1.2 Phase contrast imaging - hu-berlin.decrysta.physik.hu-berlin.de/~kirmse/pdf/2012_VL... · Tomography . Phase contrast imaging (high resolution TEM: HRTEM) Phase shift due to the

Bausch Lomb Phase Contrast Microscopy

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Interference Contrast and Phase Contrast Microscopy of Sporulation

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Contrast in TEM and STEM Amplitude and Phase contrast.

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EVALUATION OF AN IN-LINE PHASE-CONTRAST AND PHASE ...

EVALUATION OF AN IN-LINE PHASE-CONTRAST AND PHASE ...

Nonlinear Phase Contrast - University of Arizonanrw/Spring_School_2005/... · 2007. 12. 14. · Zernike Phase-Contrast Microscope The transmittance of phase object with small phase

Nonlinear Phase Contrast - University of Arizonanrw/Spring_School_2005/... · 2007. 12. 14. · Zernike Phase-Contrast Microscope The transmittance of phase object with small phase

PHASE CONTRAST Phase-Contrast Light Microscopy of Living ... · Phase-contrast light microscopy is a well-established imaging technique in cell biology. It is a powerful tool for

PHASE CONTRAST Phase-Contrast Light Microscopy of Living ... · Phase-contrast light microscopy is a well-established imaging technique in cell biology. It is a powerful tool for

Light Microscopy Bright Field Fluorescence Phase-Contrast Differential Interference Contrast (DIC)

Light Microscopy Bright Field Fluorescence Phase-Contrast Differential Interference Contrast (DIC)

Motionless phase stepping in X-ray phase contrast imaging with a

Motionless phase stepping in X-ray phase contrast imaging with a

High-resolution X-ray phase-contrast tomography from ...hofmann/MH.pdfat a limited exposure time in single-distance, propagation-based phase contrast imaging. Also, strong phase variations

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Experimental demonstration of Generalized Phase Contrast ...orbit.dtu.dk/ws/files/5504320/FD05405Ad01.pdf · Experimental demonstration of Generalized Phase Contrast based ... Experimental

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Phase contrast microscopy

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