Personal)Preferences)throughSensory)Evaluation:))...

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1 Personal Preferences through Sensory Evaluation: A Detailed Study of Perception, Association, and Difference Rebecca DeHamer April 16, 2012 NUTR 205L, Section 1 Introduction to the Science of Food

Transcript of Personal)Preferences)throughSensory)Evaluation:))...

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    Personal  Preferences  through  Sensory  Evaluation:    A  Detailed  Study  of  Perception,  Association,    

    and  Difference                                                          Rebecca  DeHamer  April  16,  2012  NUTR  205L,  Section  1  Introduction  to  the  Science  of  Food  

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    Abstract  The  decision  to  purchase  one  brand  of  product  over  another  of  the  same  product  is  based  on  multiple  factors,  one  being  the  preferences  and  opinions  of  each  consumer’s  sensory  perception.  The  purpose  of  the  sensory  evaluation  lab  was  to  teach  students  about  the  process  of  making  impartial  conclusions  during  a  sensory  tests  based  on  given  methods  and  characteristics.  Results  of    description  and  associations  of  the  food  samples  were  gathered  from  forty-‐three  student  panelists  in  a  San  Diego  State  University  food  laboratory  setting  from  an  Introduction  to  Science  of  Food  course.    The  panelist  evaluations  included:  the  color  of  beverages  based  on  a  five-‐point  scale,  difference  tests  using  samples  of  apple  juice  with  varying  degrees  of  citric  acid,  a  paired  comparison  test,  a  triangle  test,  a  scoring  test,  and  a  ranking  test.  Participation  also  included:  a  duo  trio  test  using  Nabisco  Nilla  Wafers  and  Safeway  Vanilla  Wafers,  and  a  series  of  descriptive  tests  for  appearance,  flavor,  texture,  aroma,  and  consistency  of  goldfish  crackers,  raisins,  almonds,  and  marshmallows  individually.  Results  revealed  beverage  associations  of  sweetness,  sourness,  and  naturalness  with  yellow  coloring,  and  artificiality  with  emerald  coloring.  Panelists  were  able  to  accurately  differentiate  between  sample  mixtures  of  apple  juice  with  varying  degrees  of  citric  acid,  and  most    preferred  the  pure  apple  juice  without  any  added  citric  acid.  Overall,  the  students  were  able  to  make  objective  evaluations  throughout  the  lab  procedures  allowing  thorough  experimental  data  collection.                                                  

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    Introduction  

      The  decision  to  purchase  one  brand  of  product  over  another,  is  based  on  

    multiple  factors,  including:  the  preferences  and  opinions  of  each  consumer’s  

    sensory  perception.  Food  manufacturers  spend  large  sums  of  money  every  year  

    testing  and  analyzing  data  to  promote  sales  and  progress  of  their  company.  

    Human  subject  sensory  evaluation  tests  are  used  in  research  and  development  

    departments  to  generate  and  evaluate  products  on  an  on-‐going  basis.  Objectivity  

    remains  a  key  question  due  to  the  necessity  of  such  human  subjects,  maintaining  

    their  classification  as  subjective  tests.  Sensory  tests  are  broken  down  into  two  

    categories  of  affective  tests  and  analytical  tests.  Affective  tests    focus  on  

    consumer  preference  in  specific  aspects  of  food  testing.  Analytical  tests  

    emphasize  the  objectivity  through  further  categorizing  as  discriminative  (are  

    samples  different),  and  descriptive  (how  the  samples  are  different)  tests.  

    Examples  of  discriminative  tests  include:  triangle,  duo-‐trio,  paired  comparison,  

    and  ranking  tests.  Triangle  tests  offer  three  samples,  requiring  identification  of  

    which  sample  is  specifically  different  out  of  the  three.  A  duo-‐trio  test  presents  

    three  samples  (one  set  as  a  standard),  asking  the  panelist  to  find  which  sample  is  

    the  same  as  that  standard.  A  paired  comparison  test  offers  two  samples  and  asks  

    which  has  more  of  a  known  characteristic.  A  ranking  test  presents  multiple  

    samples  and  requires  the  panelist  to  rank  from  lowest  to  highest  of  the  

    characteristic  that  is  given  (Brown  2010).  When  carrying  out  these  tests,  taste  

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    panel  instructors  must  be  strict  on  test  presentation  and  panelist’s  pallet  before  

    and  during  testing,  in  order  to  get  the  most  reliable  sensory  results.    

      Color  is  a  major  visual  factor  in  the  decision  process  of  food  consumption.  

    In  a  series  of  experimental  sensory  tests  conducted  by  Sandra  et  al.  in  2004,  

    color  was  one  of  the  sensory  factors  effecting  the  evaluation  of  crumbling  cheese  

    samples.  These  researchers  found  a  negative  correlation  reported  by  their  

    panelists  from  the  change  in  natural  color  pallet  for  each  cheese  sample.  

    Consumers  have  parameters  for  color  trends  among  different  food  products.  

    Food  companies  adhere  to  these  trends  for  successful  growth  and  sales  of  their  

    products  in  the  market.  Beverages,  often  being  displayed  in  clear  containers,  can  

    be  associated  with  color  parameters  as  well.  Vision  is  the  first  sensory  evaluating  

    process  used  to  gather  perceptions  about  the  food  product  or  beverage  being  

    presented  (Brown  2010).    

      In  a  study  conducted  by  Shankar  et  al.    in  2010,  subjects  were  asked  to  

    identify  a  beverage  after  using  the  senses  of  taste  and  smell  as  evaluation  factors.    

    Color  remained  a  strong  sensory  tool  for  the  participants  as  they  provided  data  

    that  correlated  flavors  of  Orange  and  Grape  with  corresponding  colors.  Even  

    when  instructed  to  ignore  color  cues,  participants  relied  on  color  when  taste  and  

    smell  differences  were  miniscule  (Shankar  2010).    

      Texture  can  be  equally  vital  in  the  process  of  evaluating  food  products.  

    Food  companies  focus  on  particular  pleasing  textures  specific  to  each  food  

    product  through  sensory  testing  data.  Consumers  have  strong  associations  with  

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    textures  that  may  represent  specific  food  freshness,  healthiness,  richness,  and  or  

    quality  (Brown  2010).  In  a  study  by  Ioannides  et  al.  in  2009,  subject’s  sensory  

    sensitivity  and  reliability  of  texture  was  found  to  be  as  good  as  

    electromyography  (EMG)  of  the  masticatory  muscles’  sensitivity  and  

    repeatability.    Repeated  trials  showed  sensory  results  that  matched  subjects  

    reporting  of  texture  with  the  data  collected  from  the  EMG.  The  simple  memory  of  

    known  texture  was  as  strong  as  the  results  gathered  from  the  muscle  sensitivity  

    data  when  chewing.  These  results  aide  in  the  explanation  towards  the  

    importance  of  food  sensations  such  as  texture,  consistency,  and  mouthfeel.    

       Although  these  senses  are  strong  and  show  sensitivity  to  change,  they  

    have  also  been  shown  to  be  widely  variable  and  subjective.  In  a  study  by  Marzec  

    et  al.  in  2010,  varying  degrees  and  temperatures  evaluated  the  textures  of  dried  

    apples.  Panelists  evaluated  each  apple  type,  based  on  overall  quality,  taste,  odor,  

    hardness,  and  color.  Data  revealed  that  textural  preferences  of  the  apple  food  

    products  differed  greatly  from  subject  to  subject  (Marzec  et  al.  2010).  Consumer  

    preferences  cannot  always  be  predicted  to  follow  known  patterns  of  group  liking  

    and  should  remain  one  of  the  wonderful  individualities  known  to  humans.    

      In  order  to  examine  these  parameters,  sensory  evaluations  can  be  carried  

    out  by  a  variety  of  tests.  Objectivity  and  subjectivity  remain  important  factors  in  

    the  choosing  of  each  for  specific  experimental  parameters.  A  variety  of  tests  

    were  used  within  this  analysis,  including  the  Triangle  test,  which  was  specifically  

    examined  on  its’    ability  to  be  used  as  such  in  a  study  by  McClure  et  al.  2010.  

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    McClure  et  al.  found  that  the  Triangle  test  was  effective  in  this  parameter  to  

    indicate  consumer  preference  through  discrimination  testing.  Tests  like  this  can  

    guide  Food  manufacturers  towards  the  wants  and  needs  of  their  consumers  

    when  used  appropriately,  with  data  collected  from  solid  sensory  testing  

    methods.    

      The  purpose  of  this  sensory  evaluation  lab  was  to  teach  the  student  

    participants  how  to  evaluate  foods  objectively  based  on  determined  features  

    using  descriptive  terms  and  various  taste  testing  processes.  At  the  end  of  the  

    sensory  evaluation  lab,  the  student  panelists  have  studied  how  to  properly  

    conduct  a  series  of  sensory  tests  and  can  proceed  in  these  evaluation  methods  

    with  future  work.  Students  learned  how  to  evaluate  the  color  of  the  beverage  and  

    how  this  effects  the  judgment  perception  of  that  beverage.  The  participants  

    learned  how  to  evaluate  foods  based  on  appearance,  flavor,  texture,  aroma,  and  

    consistency.  The  subjects  also  experienced  identifying  differences  in  samples  

    with  or  without  a  standard  sample  for  comparison.  Finally,  the  panelists  learned  

    to  rank  food  samples  in  an  order  of  intensity  and  preference.  This  sensory  

    evaluation  lab  included  a  beverage  color  association  test,  a  paired  comparison  

    test,  a  triangle  test,  a  scoring  test,  a  ranking  test,  a  duo-‐trio  test,  and  a  descriptive  

    test.    

    Methods  

    Panelists  

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      The  panelists  were  asked  to  answer  an  eight  point  Demographic  

    Questionnaire.  Please  refer  to  the  appendix  concerning  the  specific  content  for  

    all  questionnaires  discussed  in  this  manuscript,  if  not  specified  already  to  do  so.  

    All  questionnaires  used  in  this  evaluation  were  filled  out  by  a  single  individual  

    panelist  and  recorded  as  such  through  collection  and  the  raising  of  hands  by  an  

    individual  Teacher’s  assistant  not  included  in  the  panelist  pool.  The  panelists  

    consisted  of  43  untrained  SDSU  students  enrolled  in  the  Introduction  to  the  

    Science  of  Food  nutrition  class.  All  of  the  panelists  were  nutrition  majors.  21  of  

    the  panelists  performed  the  methods  at  a  9:00  a.m.  meeting,  and  the  remaining  

    22  performed  the  methods  at  1:00  p.m.  The  ages  of  the  panelists  ranged  from  19  

    years  old  to  43  years  old,  with  a  mean  age  of  22.8  and  a  standard  deviation  of  

    3.87.  The  majority  of  the  panelists  were  female,  88%,  and  only  12%  were  male.  

    For  student  status,  86%  were  undergraduates,  14%  were  graduate  students.  For  

    marital  status,  88%  were  never  married,  9%  were  married,  and  2%  were  

    divorced.  For  living  arrangements,  5%  were  living  alone,  28%  were  living  with  

    one  roommate,  and  67%  were  living  with  two  or  more  roommates.  Concerning  

    allergies,  88%  did  not  identify  with,  and  12%  did  identify  with.  Additionally,  

    98%  were  nonsmokers,  and  2%  were  smokers.    

    Environment  

      The  evaluation  of  the  Standards  of  Identity  and  Standards  of  Reasonable  

    Quality  for  fruit  cocktail  took  place  at  San  Diego  State  University’s  food  science  

    lab  room  (West  Commons  203).  All  procedures  were  conducted  under  

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    reasonably  controlled  conditions  (light  control,  humidity,  and  temperature).  

    Lighting  was  a  constant  dim,  sufficient  for  working  conditions;  humidity  and  

    temperature  were  within  a  normal  range  (40-‐45%  and  20-‐24°C).  The  room  set-‐

    up  had  standard  academic  seating  arrangements  (individual  desk-‐chairs)  with  5  

    rows  of  6  in  the  center  surrounded  by  kitchen  lab  equipment.  On  two  sides  of  the  

    desk  arrangement  were  sets  of  stove  top-‐tables  (3  per  side)  housing  two  per  

    table.  In  addition,  were  3  sinks  per  side  for  hand  washing,  food  washing,  and  

    dishwashing,  accordingly.  At  the  front  of  the  room  was  a  corner  dedicated  to  

    ovens,  housing  6  convection-‐stacked  2  per  unit-‐all  below  a  ventilation  structure.  

    At  the  center  of  the  front  and  rear  of  the  room  are  larger  desks  for  instruction  

    and  multi-‐use.  Cabinetry  surrounds  the  whole  room  for  storage.  The  lab  room  

    has  no  windows,  but  two  doors  located  at  the  front  corners.    Doors  were  kept  

    shut  during  the  procedure.    

    Color  Association/Beverage  Perception  

      The  panelists  were  presented  with  five  different  beverages  of  varying  

    colors  and  were  asked  to  rank  for  certain  parameters.  Each  participant  recorded  

    his  or  her  results  on  the  Sensory  Testing  Beverage  Questionnaire  (see  

    Appendix).  The  drink  color  was  the  only  ranking  evaluation  characteristic.  The  

    test  was  ranked  on  a  5-‐point  scale,  with  five  being  the  most,  four  being  almost  

    the  most,  three  being  in  the  middle,  two  being  almost  least,  and  one  being  the  

    least.  The  colors  consisted  of  light  yellow,  dark  yellow,  chartreuse,  dark  

    chartreuse,  and  emerald  green  placed  right  to  left,  respectively  at  the  front  of  the  

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    room  in  uniform  beaters.  The  panelists  gave  each  beverage  a  rating  of  1-‐5  for  the  

    perceived  sweetness,  sourness,  artificiality,  naturalness,  and  which  drink  the  

    student  preferred  or  disliked  the  most.  In  addition  to  rating,  the  participants  

    were  asked  to  record  preferred  temperature  of  the  beverage  as  hot,  war,  tepid,  

    or  cold.  Lastly,  the  panelists  recorded  whether  or  not  they  would  drink  the  

    beverages.  The  information  logged  by  the  subjects  on  the  Sensory  Testing  

    Beverage  Questionnaire  was  tallied  and  compiled  by  the  lab  instructor  and  

    assistant  using  a  show-‐of-‐hands  method.    

    Difference  Tests  

      The  panelists  conducted  a  series  of  difference  tests,  including  a  paired  

    comparison  test,  a  triangle  test,  a  scoring  test,  a  ranking  test,  and  a  duo-‐trio  test.  

    The  paired  comparison,  triangle,  scoring  and  ranking  tests  used  samples  of  pure  

    apple  juice  and  mixtures  of  apple  juice  with  added  citric  acid.  The  duo-‐trio  test  

    was  conducted  using  vanilla  wafer  cookies.  All  of  the  products  were  served  in  

    small,  white,  uniform  soufflé  cups  and  panelists  tasted  each  food  individually.  

    Water  was  available  for  the  panelists  to  cleanse  their  pallet,  and  was  served  in  

    white  Styrofoam  cups.  Panelists  recorded  their  evaluation  on  Sensory  Evaluation  

    Duo-‐Trio  Test  and  Scoring  or  Rating  Test  sheet  (see  Appendix).  

      The  paired  comparison  test  measured  which  sample  of  apple  juice  was  

    the  sourest.  The  Panelists  tasted  two  samples  labeled:  635T1  and  573T2.  All  

    participants  walked  to  the  front  of  the  lab  to  take  small  samples  and  refrained  

    from  taking  the  sample  until  all  participants  had  theirs  to  partake  together.  They  

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    recorded  their  perceived  results  on  the  Paired  Comparison  Test  table,  located  in  

    the  Nutrition  205  lab  manual  (see  Appendix).  The  sample  coded  635T1  was  pure  

    apple  juice  and  the  sample  coded  573T2  was  a  mixture  of  apple  juice  and  1%  

    citric  acid.  For  the  triangle  test,  panelists  identified  which  two  samples  of  apple  

    juice  were  alike  and  which  were  different.  The  samples  were  labeled:  777C1,  

    542E2,  and  112H9.  Students  recorded  their  results  on  the  Triangle  Test  table  in  

    the  Nutrition  205  lab  manual  (see  Appendix).  Samples  777C1  and  542E2  were  

    both  pure  apple  juice.  Sample  112H9  was  different  from  the  others,  containing  a  

    mixture  of  apple  juice  and  1%  citric  acid.    

      For  the  scoring  test,  panelists  were  given  three  samples  of  apple  juice  to  

    taste.  There  was  one  reference  sample,  labeled  0110.  This  sample  was  given  a  

    ranking  score  of  4  on  a  1-‐7  scale,  with  1  being  the  sourest,  and  7  being  the  

    sweetest.  Samples  420M  and  S723  were  unknown  to  the  panelists  who  had  to  

    place  them  on  the  scale  by  their  taste  interpretation.  The  participants  recorded  

    their  ranking  son  the  Sensory  Evaluation  sheet  (Appendix  B).  The  reference  

    sample  (0110)  had  2.5%  citric  acid,  the  unknown  sample  (420M)  had  1%  citric  

    acid,  and  the  unknown  sample  (S723)  had  5%  citric  acid.  

      In  the  ranking  test,  the  panelists  were  given  five  samples  of  apple  juice.  

    They  ranked  the  five  samples  in  order  of  intensity  based  on  sourness;  1  as  the  

    sourest  and  5  as  the  least  sour.  The  participants  ranked  the  samples  in  order  of  

    preference,  following  the  same  scale.  The  results  were  recorded  on  the  Ranking  

    Test  table  in  the  Nutrition  205  lab  manual  (see  Appendix).  Sample  495P2  was  

  •   11  

    pure  apple  juice,  543K8  had  1%  citric  acid,  695F8  had  2.5%  citric  acid,  192L3  

    had  5%  citric  acid,  and  555D7  had  10%  citric  acid.  

      Panelists  were  presented  with  three  vanilla  wafer  cookies  during  the  duo-‐

    trio  test.  Sample  8175  was  set  as  the  standard  as  Nabisco  Nilla  Wafers.  The  

    panelists  tasted  samples  6104  and  1108,  and  recorded  which  vanilla  wafer  

    differed  from  the  standard  and  why.  Sample  1108  was  the  same  as  the  standard,  

    and  6104  was  the  Safeway  brand  vanilla  wafers.  The  panelists  recorded  their  

    findings  on  the  sensory  evaluation  sheet  (see  Appendix).    

    Descriptive  Tests  

      The  descriptive  tests  required  the  panelists  to  evaluate  four  different  food  

    products  using  descriptive  terms.  Each  sample  was  evaluated  based  on  its  

    appearance,  aroma,  flavor,  texture,  and  consistency.  The  students  were  given  2-‐3  

    bites  of  marshmallows,  raisins,  goldfish  crackers,  and  almonds  that  were  all  

    presented  in  uniform,  white,  soufflé  cups.  The  panelists  were  provided  with  a  list  

    of  descriptive  terms  that  they  were  allowed  to  use  for  each  characteristics  (see  

    Appendix).    

    Statistical  Analysis  

      The  course  instructor  and  lab  assistant  recorded  the  results  of  each  test  

    from  all  of  the  participants.  They  were  able  to  record  the  beverage  color  and  

    association  test,  scoring  test,  ranking  test,  descriptive  test,    paired  comparison,  

    triangle,  and  duo-‐trio  tests  using  a  show-‐of-‐hands  method  as  well  as  

  •   12  

    questionnaire  techniques.  All  of  this  data  was  entered  and  compiled  into  an  excel  

    spreadsheet.  The  results  from  the  two  sections  were  combined.    

    Results  

    Color  Association  /Beverage  Perception  

      All  the  panelists  participated  in  beverage  association  activity.  When  asked  

    which  beverage  they  thought  was  the  sweetest,  37.2%  of  the  panelists  recorded  

    light  yellow,  30.2%  dark  yellow,  18.6%  emerald,  7%  dark  chartreuse,  and  2.3%  

    chartreuse  (Figure  1).    

     

      Twenty-‐eight  percent  of  the  panelists  found  the  light  yellow  beverage  was  

    the  sourest,  25.6%  dark  chartreuse,  16.3%  dark  yellow,  14%  chartreuse,  and  

    11.6%  emerald  (Figure  2).  

    37.2%  

    30.2%  

    2.3%  7.0%  

    18.6%  

    Fg.  1  -‐  Sweetest  Beverage  

    Light  Yellow  

    Dark  Yellow  

    Chartreuse  

    Dark  Chartreuse  

    Emerald  

  •   13  

     

      Seventy-‐seven  percent  of  the  panelists  recorded  the  emerald  beverage  as  

    most  artificial  looking,  11.6%  dark  yellow,  and  2.3%  each  as    chartreuse,  dark  

    chartreuse,  and  light  yellow.  Eighty-‐eight  and  a  half  percent  of  the  panelists  

    found  the  light  yellow  beverage  to  be  most  natural,  4.7%  found  emerald  as  such,  

    and  2.3%  found  chartreuse  as  such.  Sixty-‐seven  and  a  half  percent  of  panelists  

    had  a  preference  for  the  light  yellow  beverage,  11.6%  for  chartreuse,  7%  dark  

    yellow,    and  4.7%  for  the  dark  chartreuse  and  emerald.  Sixty  and  a  half  percent  

    of  the  panelists  found  most  dislike  in  the  emerald  beverage,  18.6%  with  the  dark  

    yellow,  7%  with  the  dark  chartreuse  and  the  light  yellow,  and  2.3%  the  

    chartreuse  (Figure  3).  

     

     

     

     

    27.9%  

    16.3%  14.0%  

    25.6%  

    11.6%  

    Fg.  2  -‐Sourest  Beverage  

    Light  Yellow  

    Dark  Yellow  

    Chartreuse  

    Dark  Chartreuse  

    Emerald  

  •   14  

    Fg.  3-‐Most  Artificial,  Natural,  Preferred  and  Disliked  of  Beverages  

     

    The  panelists  preferred  all  the  beverages  at  varying  temperatures.  In  regards  to  

    the  light  yellow  beverage,  95.3%  favored  it  cold,  11.6%  tepid.  In  regards  to  the  

    dark  yellow  beverage,  95.3%  favored  it  cold,  7%  tepid,  and  2.3%  hot.  In  regards  

    to  the  chartreuse  and  dark  chartreuse  colored  beverage,  95.3%  favored  it  cold,  

    and  2.3%  warm.  Lastly,  95.3%  of  the  panelists  favored  the  emerald  colored  

    beverage  at  a  temperature  of  cold,  and  2.3%  hot  (Figure  4).    

     

     

     

     

     

     

    2.3%

    88.4%

    67.4%

    7.0%

    11.6%

    0.0%

    7.0%

    18.6%

    2.3% 2.3%

    11.6%

    2.3%2.3%0.0%

    4.7%7.0%

    76.7%

    4.7% 4.7%

    60.5%

    0.0%

    10.0%

    20.0%

    30.0%

    40.0%

    50.0%

    60.0%

    70.0%

    80.0%

    90.0%

    100.0%

    most  artificial most  natural most  prefer most  dislike

    Light  YellowDark  YellowChartreuseDark  ChartreuseEmerald

  •   15  

    Fg.  4-‐Preferred  Temperature  of  Beverages  

     

    When  asked  if  they  would  drink  the  beverages,  81.4%  of  the  panelists  would  

    consume  the  light  yellow  beverage,  58.1%  the  chartreuse,  ,  46.5%  the  dark  

    yellow,  37.2%  the  dark  chartreuse,  and  25.6%  the  emerald  beverage  (Figure  5).  

     

     

    2.3%  

    2.3%  2.3%   2.3%  

    11.6%  

    7.0%  

    82%  

    84%  

    86%  

    88%  

    90%  

    92%  

    94%  

    96%  

    98%  

    100%  

    Light  Yellow  

    Dark  Yellow  Chartreuse   Dark  Chartreuse  

    Emerald  

    tepid  

    warm  

    hot  

    cold    

    81.4%  

    46.5%  58.1%  

    37.2%  

    25.6%  

    Fg.  5  -‐  Drink  Beverage  =  "Yes"  

    Light  Yellow  

    Dark  Yellow  

    Chartreuse  

    Dark  Chartreuse  

    Emerald  

  •   16  

    Paired  Comparison  Test  

      One  hundred  percent  of  the  panelists  who  participated  in  the  paired  

    comparison  test  identified  the  mixture  of  apple  juice  with  1%  citric  acid  to  be  

    sourer  than  the  pure  apple  juice.    

    Triangle  Test  

      Ninety-‐five  percent  of  panelists  who  participated  in  the  triangle  test  

    identified  the  mixture  of  apple  juice  and  1%  citric  acid  as  being  different  from  

    the  two  samples  of  pure  apple  juice.    

    Ranking  Test  

      The  43  panelists  who  participated  were  required  to  put  the  samples  in  

    order  of  sourness.  Ninety  percent  ranked  the  mixture  of  apple  juice  with  10%  

    citric  acid  as  the  sourest  and  9.3%  ranked  the  mixture  of  apple  juice  with  5%  

    citric  acid  as  such.  Ninety-‐five  percent  of  the  subjects  ranked  the  pure  apple  juice  

    as  the  least  sour,  and  4.7%  ranked  the  mixture  of  apple  juice  with  1%  citric  acid  

    as  such  (Table  1).  

     Table  1.  Ranking  Test:  Degree  of  Astringency  showing  %  of  panelists  for  extreme  values           %  of  Citric  Acid  in  Apple  Juice  

    Degree  of  

    Astringency  

    0%  

    495P2  

    1%  

    543K8  

    2.5%  

    695F8  

    5%  

    192L3  

    10%  

    555D7  

    1:  MOST  SOUR   0%   0%   0%   9.3%   90.7%  

    5:  LEAST  SOUR   95.3%   4.7%   0%   0%   0%  

     

  •   17  

      Panelists  were  also  asked  which  sample  they  preferred  the  most  and  the  

    least.  Sixty-‐five  and  a  half  percent  preferred  the  pure  apple  juice  overall,  20.9%  

    the  mixture  with  apple  juice  with  5%  citric  acid,  9.3%  the  mixture  of  apple  juice  

    with  2.5%  citric  acid,  7%  the  mixture  of  apple  juice  with  1%  citric  acid,  and  2.3%  

    the  mixture  of  apple  juice  with  10%  (Table  2).  

     Table  2.  Ranking  Test:  Degree  of  Preference  showing  %  of  panelists  for  extreme  values           %  of  Citric  Acid  in  Apple  Juice  

    Degree  of  

    Astringency  

    0%  

    495P2  

    1%  

    543K8  

    2.5%  

    695F8  

    5%  

    192L3  

    10%  

    555D7  

    1:  MOST  

    PREFERRED  

    60.6%   7%   9.3%   20.9%   2.3%  

    5:  LEAST  

    PREFERRED  

    2.3%   11.6%   2.3%   4.7%   79.1%  

     

    Duo-‐Trio  Test  

      Ninety-‐seven  percent  of  the  panelists  chose  the  Safeway  Vanilla  Wafers  as  

    the  sample  that  was  different  in  the  duo-‐trio  test.  They  had  various  reasons  for  

    making  their  decisions  including:  26.2%  judged  them  as  stale  in  comparison  to  

    the  other,  14.3%  judged  them  harder  in  texture  than  the  Nabisco  Nilla  Wafers  

    and  having  different  taste,    11.9%  judged  them  as  less  crunchy,  and  varying  

    other  reasons  (Figure  6).  

  •   18  

     

    Scoring  Test  

      Forty-‐three  panelists  participated  in  the  scoring  test.  The  mixture  of  apple  

    juice  with  5%  citric  acid  scored  1  with  58.1%  of  the  subjects,  and  2  with  41.9%.    

    The  mixture  of  apple  juice  with  1%  citric  acid  scored  2  and  4  each  with  2.3%  of  

    the  subjects,  5  with  11.6%,  6  with  62.8%,  and  7  with  20.9%  of  the  subjects.  

    Descriptive  Tests  

      The  panelists  had  to  evaluate  the  taste  of  goldfish  crackers,  raisins,  

    almonds,  and  marshmallows  on  the  characteristics  of  flavor,  texture,  aroma,  

    consistency,  and  mouthfeel.    They  further  assessed  their  appearance  via  a  list  of  

    descriptive  terms  (Appendix).  All  42  panelists  participated  in  the  goldfish  

    cracker  descriptive  test.  Thirty-‐one  percent  of  the  participants  described  the  

    cracker’s  appearance  as  golden-‐brown,  26%  as  dry,  9.5%  each  rough  and  

    rounded,  7.1%  each  symmetrical  and  as  asymmetrical,  4.8%  grainy,  and  2.4%  

    0.0%   5.0%  10.0%  15.0%  20.0%  25.0%  30.0%  

    dryness  

    less  vanilla  

    stale  

    texture  

    ilaky  

    hollow  

    Sweet  

    0.0%  11.9%  

    7.1%  0.0%  

    26.2%  14.3%  

    9.5%  7.1%  

    2.4%  14.3%  

    2.4%  0.0%  

    4.8%  

    Fg.  6  -‐  97.6%  of  Participants  chose  Safeway  based  on  these  characterists  

  •   19  

    each  puffy  and  dull.  One  hundred  percent  of  the  panelists  described  the  flavor  as  

    salty.  Fifty  percent  of  the  participants  portrayed  the  texture  as  crunchy,  43%  as  

    crisp,  and  2.4%  each  as  hard,  gritty,  and  mealy.  Seventy-‐eight  and  a  half  percent  

    of  the  panelists  described  the  aroma  as  flavery,  9.5%  as  spicy,  7.1%  had  no  

    aroma,  and  4.8%  as  sweet.  Sixty-‐six  and  a  half  percent  of  the  participants  

    designated  the  consistency  as  cheesy,  23.8%  as  thin,  7.1%  as  thick,  and  2.4%  as  

    viscous.    Sixty-‐eight  percent  of  the  panelists  found  the  mouthfeel  of  the  crackers  

    as  crunchy,  18.2%  as  crisp,  and  13.6  gritty.    

      All  42  panelists  participated  in  the  raisin  descriptive  test.  Thirty-‐six  

    percent  of  the  panelists  described  the  raisin’s  appearance  as  dry,  29%  as  rough,  

    9.5%  each  asymmetrical  and  dark,  7.15  as  smooth,  4.8%  as  sticky,  and  2.4%  each  

    dull  and  puffy.    Fifty-‐two  percent  of  the  participants  designated  the  flavor  as  

    sweet,  33.3%  as  fruity,  9.5%  as  bitter,  and  2.4%  each  as  pasty  and  musky.  Forty  

    percent  of  the  panelists  portrayed  the  texture  as  chewy,  24%  as  gummy,  14%  as  

    gritty,  9.5%  as  lumpy,  4.8%  rubbery,  and  2.4%  each  as  rough,  firm,  and  crunchy.  

    Seventy-‐four  percent  of  the  participants  reported  the  aroma  as  fruity,  21.4%  as  

    sweet,  and  2.4%  each  of  flavory  and  sour.  Sixty-‐four  percent  of  the  participants  

    described  the  consistency  as  gummy,  16.7%  as  rubbery,  11.9  as  thin,  and  7.1%  as  

    thick.  Fifty-‐nine  percent  of  the  participants  found  the  mouthfeel  to  be  sticky,  

    22.7%  as  gritty,  9.1%  as  slimy,  and  4.5%  each  as  crisp  and  smooth.  

      Only  41  panelists  participated  in  the  almond  descriptive  test.  Thirty-‐two  

    percent  of  the  panelists  described  the  almond’s  appearance  as  golden-‐brown,  

  •   20  

    17%  as  dry,  15%  as  light  brown,  9.8%  as  rough,  4.9%  each  as  rounded,  smooth,  

    dull,  and  grainy,  and  2.4%  as  sticky,  symmetrical,  and  asymmetrical.  Eighty-‐eight  

    percent  of  the  participants  designated  the  flavor  as  nutty,  9.8%  as  flat,  and  2.4%  

    as  sweet.  Forty-‐one  percent  of  the  panelists  portrayed  the  texture  as  hard,  27%  

    as  crunchy,  20%  as  firm,  and  2.4%  each  as  velvety,  crisp,  rough,  gritty,  and  

    tender.  Forty-‐one  and  a  half  percent  of  the  participants  reported  the  aroma  as  

    nonexistent,  26.8%  as  flavery,  17.1%  as  fruity,  7.3%  as  spicy,  4.9%  as  sweet,  and  

    2.4%  as  sour.  Ninety  percent  of  the  participants  described  the  consistency  as  

    thick,  4.9%  as  butter,  and  2.4%  each  as  thin,  and  rubbery.  Seventy-‐seven  percent  

    of  the  participants  found  the  mouthfeel  to  be  crunchy,  13.6%  as  gritty,  and  4.5%  

    each  as  crisp  and  smooth.  

      All  42  panelists  participated  in  the  marshmallow  descriptive  test.  Thirty-‐

    eight  percent  of  the  panelists  described  the  marshmallow’s  appearance  as  puffy,  

    33%  as  rounded,  9.5%  as  symmetrical,  7.1%  as  creamy,  4.8%  each  as  dull  and  

    dry,  and  2.4%  as  fine.  Ninety-‐seven  and  a  half  percent  of  the  participants  

    designated  the  flavor  as  sweet,  and  2.4%  as  pasty.  Thirty-‐six  percent  of  the  

    panelists  portrayed  the  texture  as  springy,  19%  as  velvety,    12%  as  chewy,  9.5%  

    gummy,  7.1%  each  as  gelatinized  and  moist,  4.8%  as  crisp,  and  2.4%  each  as  

    rough  and  rubbery.  Ninety-‐seven  and  a  half  percent  of  the  participants  reported  

    the  aroma  as  sweet,  and  2.4%  as  flowery.  Seventy-‐eight  and  a  half  percent  of  the  

    participants  described  the  consistency  as  gummy,  11.9%  as  rubbery,  4.8%  as  

    thick,  and  12.4%  each  as  butter  or  thin.  Sixty-‐three  and  a  half  of  the  participants  

  •   21  

    found  the  mouthfeel  to  be  smooth,  27.3%  as  sticky,  and  4.5%  each  as  slimy  and  

    gritty.  

    Discussion  

    Color  Association  /Beverage  Perception  

      Panelist  beverage  perception  and  opinion  is  shown  more  prominently  

    with  each  additional  analysis  and  association.  Panelists  perceived  the  yellow-‐

    colored  beverages  (light  yellow  and  dark  yellow)  as  being  sweeter  than  the  

    others.  This  may  be  due  to  an  association  with  lemonade  and  a  sweet  correlation  

    to  that  beverage  based  on  color  similarities.  For  sourness,  results  conflicted  with  

    the  sweet  data,  finding  the  light  yellow  ranked  high  along  with  the  dark  

    chartreuse  colored  beverage.  Participants  could  have  been  overwhelmed  with  

    the  taste  overflow  of  sample,  which  could  account  for  the  contradictory  figures.    

    A  smaller  sample  of  beverages  or  a  longer  time  in-‐between  sampling  could  

    remedy  this  if  the  experiment  were  to  be  repeated.  Similar  conduction  of  a  

    sensory  test  was  witnessed  in  the  Shankar  et  al.  2010  study  where  the  subjects  

    were  aware  of  color  conditions  with  beverage  choice.  All  results  show  color  as  a  

    strong  deciding  factor  in  beverage  classification.    

      The  emerald  beverage  was  found  to  be  most  artificial  and  most  disliked  

    by  the  participants.  The  artificiality  could  be  based  on  the  unusual  brightness  of  

    the  emerald  green  color  and  lack  of  an  association  with  another  beverage.  This  

    could  lead  to  the  ranking  of  most  disliked  by  creating  a  negative  association  with  

    that  color  disparity  in  the  panelist’s  memory.    

  •   22  

      The  light  yellow  beverage  was  found  to  be  most  natural  and  most  

    preferred  by  the  participants.  The  recognition  of  the  light  yellow  color  most  

    likely  was  due  to  an  association  with  a  similar  sport  drink  color  or  lemonade  

    color.  This  may  have  lead  participants  to  a  ranking  of  most  preferred  due  to  a  

    feeling  of  safeness  with  the  color  from  a  basis  of  memory  association.    

       For  temperature,  all  panelists  preferred  the  beverages  cold  overall.  Some  

    participants  included  tepid  temperatures  fairly  high,  but  the  coldest  temperature  

    remained  the  preference  throughout  the  color  variability.  Different  countries  

    around  the  world  have  patterns  and  customs  that  guide  drink  temperature.  In  

    the  United  States  (where  this  experiment  was  conducted),  beverage  temperature    

    in  this  color  range,  is  dominated  by  a  cold  preference  and  is  perhaps  the  major  

    reason  for  the  data  representation  here.  Future  research  in  this  area  could  

    include  other  colors  that  are  known  to  vary  in  temperature,  including:  black,  

    brown,  or  a  lighter  brown.    

    Paired  Comparison  Test  

      All  the  panelists  identified  the  correct  sample  in  the  paired  comparison  

    test.  Possibly  the  samples  were  easy  to  differentiate  by  that  timing  of  the  testing.  

    In  order  to  account  for  this,  a  repeated  experiment  could  change  the  order  of  the  

    testing  to  check  the  accuracy  of  this  data.  If  repeated  results  were  found  to  show  

    100%  correct  identification,  verification  of  these  outcomes  would  be  established.    

    Triangle  Test  

  •   23  

      All  participants  were  able  to  identify  the  different  samples  in  the  triangle  

    test.  In  order  to  validate  the  accuracy  of  this  data,  repeated  experimentation  

    could  be  used  where  the  order  of  testing  was  reversed  or  mixed.  This  would  

    allow  measures  to  be  seen  from  multiple  testing  organizational  methods.  

    Furthermore,  not  all  panelists  were  able  to  participate  due  to  dietary  

    restrictions.  In  further  work,  questionnaires  should  be  able  to  separate  out  

    candidates  that  cannot  participate  in  the  full  process.    

    Scoring  Test  

      The  majority  of  the  participants  were  able  to  correctly  place  the  samples  

    of  apple  juice  on  the  appropriate  locations  on  the  scale.  The  majority  of  the  

    panelists  scored  the  pure  apple  juice  as  the  sweetest  and  the  sourest  as  the  

    mixtures  of  apple  juice  and  a  10%  citric  acid  or  5%  citric  acid  component.  The  

    ability  to  discern  the  two  highest  citric  acid  contenders  was  not  uniform  amongst  

    the  participants,  most  likely  accounting  for  the  small  variability  in  the  scoring  

    found  here.    This  could  be  due  to  individual  variances  found  from  panelist  tasting  

    ability;  as  it  is  a  common  characteristic  to  have  differing  ranges  of  taste  

    sensation  (Brown  2012).  These  results  could  also  possibly  be  due  to  an  

    additional  issue  of  taste  fatigue,  since  there  were  5  samples  consecutively  tested.  

    If  repeated,  the  experiment  should  lower  the  sample  number  to  deter  this  affect.    

    Ranking  Test  

      All  panelists  participated  in  the  ranking  test,  with  the  majority  able  to  

    correctly  identify  the  most  and  least  sour  apple  juice  mixtures.  The  greatest  

  •   24  

    amount  of  panelists  preferred  the  pure  apple  juice  the  most  and  the  mixture  of  

    apple  juice  and  10%  citric  acid  the  least.  As  previously  stated,  to  prevent  taste  

    fatigue,  the  panelists  could  be  given  fewer  samples  to  compare  with  more  time  in  

    between  to  deter  a  overflow  effect  of  the  samples.    

    Duo-‐Trio  Test  

      Almost  all  of  the  panelists  participated  in  the  duo-‐trio  test,  and  those  who  

    did  were  able  to  correctly  identify  that  the  Safeway  Vanilla  Wafers  were  different  

    from  the  standard  sample.  Incorrect  identification  of  the  sample  could  have  been  

    due  to  experiencing  taste  fatigue,  recorded  incorrect  results,  or  having  been  

    given  a  defective  sample.  The  panelists  believed  that  the  wafers  were  different  

    based  on  varying  reasons  mainly  due  to  personal  preference.  Further  tests  could  

    thoroughly  follow  recording  procedures,  check  each  sample  before  given  to  

    panelists,  and  possibly  lower  the  amount  of  descriptive  terms  in  order  to  prevent  

    these  variances.    

    Descriptive  Tests  

      The  majority  of  the  panelists  participated  in  the  goldfish  cracker  

    descriptive  test.  If  this  study  were  to  be  conducted  again,  the  organizer  could  

    question  participants  before  involvement  in  order  to  omit  those  who  cannot  

    participate  in  all  aspects  of  the  experiment.  The  crackers’  appearance  was  found  

    to  be  golden-‐brown,    rough,  rounded,  asymmetrical,  symmetrical,    grainy,  puffy,  

    or  dull.  In  order  to  minimize  the  difference  seen  in  these  results,  the  organizers  

    could  present  a  shorter  list  of  descriptive  terms,  which  would  assist  the  panelists  

  •   25  

    in  honing  their  sensory  evaluation  skill.  In  addition,  there  are  normal  variances  

    in  the  way  the  crackers  were  viewed,  possibly  from  one  angle  while  others  view  

    from  a  different  angle.  If  this  examination  was  repeated  on  crackers,  a  uniform  

    shaped  product  could  be  chosen  in  place  of  the  fish  shape.  Test  organizers  could  

    also  check  for  regularity  of  size  and  shape  for  uniformity  of  each  cracker.  

    Consistency,  mouthfeel  and  texture  recordings  also  found  similar  results  of  

    varying  responses,  with  one  exception  of  the  taste  with  100%  reporting  them  as  

    salty.    

      All    of  the  panelists  participated  in  the  raisin  and  almost  all  participated  in  

    the  almond  descriptive  tests.  The  majority  found  the  raisins  to  by  sticky,  sweet,  

    chewy,  and  fruity.  The  majority  also  found  the  almond  to  be  golden-‐brown,  

    nutty,  hard,  brittle,  and  odorless.  In  the  future,  a  more  fragrant  nut  could  replace  

    the  almond  so  there  is  something  to  report  by  panelists  for  odor.  As  previously  

    stated,  performing  some  pre-‐selection  questions  can  remedy    the  lower  level  of  

    participation  by  the  subject  panelists.  This  can  be  conducted  by  simply  omitting  

    panelists  who  have  a  nut  allergy  or  diet  condition  that  keeps  them  from  

    participating  in  all  the  testing  procedures.  

      As  with  the  other  tests,  not  all  panelists  participated  in  the  marshmallow  

    descriptive  test.  Panelists  found  them  puffy,  rounded,  symmetrical,  creamy,  dull,  

    dry,  and  or  fine.  Flavor  was  found  to  be  sweet,  or  pasty,  and  texture  as  springy,  

    velvety,  chewy,  gummy,  gelatinized,  and  or  moist,  plus  others.  Varied  responses  

  •   26  

    could  be  minimized  (as  stated  previously)  by  having  a  shorter  recommended  

    word  list.    

      The  results  of  these  tests  all  show  that  panelists  were  able  to  evaluate  

    food  samples  objectively  based  on  given  characteristics.  Any  sensory  

    examinations  that  had  panelists  omitting  from  participating  was  due  to  specific  

    diet  parameters  that  kept  them  from  consuming  those  specific  foods.  In  the  end,  

    participants  were  able  to  meet  all  of  the  goals  set  forth  by  the  organizers  of  this  

    data  collection.  Students  could  properly  re-‐administer  a  sensory  test  on  their  

    own,  analyzing  color  associations  and  descriptive  properties  of  food  samples.  

    Major  changes  in  a  repeat  experimental  process  should  include  the  before  

    mentioned  suggestions  including:  shorter  recommended  descriptive  word  

    tables,  pre-‐qualifying  panelist  questionnaires,  thorough  following  of  collection  

    and  recording  procedures,  and  possibly  an  education  process  to  assist  panelists  

    in  how  to  properly  test  for  sensory  evaluations.  This  lab  experiment  has  

    introduced  the  students  involved  to  sensory  perception  testing  and  taught  all  

    how  to  successfully  repeat  and  carry  out  such  tasks  in  future  research.    

                           

  •   27  

    NUTR205     Demographic  Questionnaire    Name______________________________  Group  Number  _____    Section  Number_____      Please  answer  the  following  questions.    Note:  The  numbers  in  parentheses  are  used  for  recording  data  in  the  spreadsheet  only.    Your  instructor  will  explain  this  process  in  further  detail  when  necessary.    

    1. Your  age,  in  years:  __________  

    2. Your  gender  (circle):     Male(1)     Female(2)  

    3. Your  major  (circle/fill-‐in):       F/N     Other____________________  

    4. Student  Status  (circle):     Undergraduate  (1)   Graduate(2)  

    5. Marital  Status  (circle):     never  married(1)   married  (2)   widowed(3)  

            divorced(4)  

    6. Living  arrangements  (circle):   alone(1)   1  roommate(2)          

            2+roommates(3)  

    7. Do  you  smoke  (circle):     Yes(1)     No(2)  

    8. Do  you  have  any  food  allergies  (circle)?    If  yes  please  specify     Yes(1)    

                      No(2)  

    ________________________________________________________________________  

                           

  •   28  

    NUTR  205   Sensory  Testing  Beverage  Questionnaire      Name______________________________  Group  Number  _____    Section  Number_____  Do  you  drink  Apple  Juice                                                        yes                                                                                                        No  For  each  of  the  parameters  (sweetness,  sourness,  naturalness,  artificiality,  preference  and  dislike)  give  the  beverage  with  the  most  a  rating  of  5.    Give  the  beverage  with  the  least  a  ranking  of  1.    Similarly  the  one  that  is  almost  as  sweet  as  the  sweetest  beverage  receives  a  ranking  of  4,  while  the  next  to  least  sweet  receives  a  ranking  of  2.    The  middle  sample  will  be  given  a  rating  of  3.        

    5     4     3     2     1                                                                                                                                            For  stating  what  temperature  you  would  the  beverage  at,  state  whether  you  would  drink  the  beverage  hot,  warm,  tepid  or  cold.    Fill  in  the  cell  with  your  choice.      Complete  the  question  “Would  you  drink  it?”  as  either  yes  or  not  ONLY!    Please  do  not  use  words  like  sometimes  or  maybe.      

    Beverage  Color  and  Associations  with  Other  parameters  Parameter   1  light  

    yellow  2  dark  yellow  

    3  chartreuse  

    4  dark  chartreuse  

    5  emerald  green  

    Sweetness            

    Sourness            

    Artificiality            

    Naturalness            

    Prefer            

    Dislike            

    At  what  temp  would  you  drink  

    it?  

             

    Would  you  drink  it?  

             

                         

    Most   Least  

  •   29  

    DESCRIPTIVE  TERMS  To  use  with  Experiment  #3,  Letter  A,  Table  A-‐1  in  Lab  Book.  When  evaluating  the  foods  in  this  experiment,  select  one  of  the  words  from  this  list  for  the  appropriate  category.    Appearance               Texture              Symmetrical               Crisp  Asymmetrical               Velvety  Rounded               Rough  Dry                 Hard  Golden  brown               Firm  Light  brown  smooth             Thin  Rough                 Viscous  Puffy  dark               Springy  Creamy               Gritty  Dull                 Gummy  Fine                 Adhesive  Grainy                 Moist  Sticky                 Tender  Glossy                 Chewy                   Lumpy  Flavor                 Rubbery                   Crunchy  Sweet                 Gelatinized  Bitter                 Mealy  Sour                  Salty  Flat                 Mouthfeel  Rancid  Pasty                 Crisp                   Sticky          Flowery               Slimy  Fruity                 Gritty  Musky                 Slick  Nutty                 Crunchy                   Smooth  Aroma                   Consistency  Spicy    Flavery                 Butter  Fruity                 Cheezy  Sweet                 Viscous  Sour                 Thick                   Thin                   Rubbery                   Gummy              

       

  •   30  

    NUTR205   Sensory  Evaluation      Name______________________________  Group  Number  _____  Section  Number_____    Duo-‐Trio  Test    Determine  which  cookie  sample  differs  from  the  standard  presented  first.    Standard  =  8175           Sample  =  6104        

           Sample  =  1108          

    Which  sample  differs  from  the  standard?  __________  In  your  opinion  what  is  the  major  difference?  ____________________    Scoring  or  Rating  Test    The  reference  sample,  0110,  has  been  given  an  arbitrary  score  of  4.    Rate  the  sour  intensity  of  the  other  two  samples  relative  to  the  reference.    Sample  420M                                           Sample  S723  

       

    More  sour  1  .________                  2.  ________                  3.________                                0110              4.________                    5.________                  6._________                          Less  sour      7.________  

                           

  •   31  

    KEY  FOR  SENSORY    

    EXPERIMENT 1 - ASSOCIATION OF COLOR IN BEVERAGES WITH SOURNESS, SWEETNESS, AND PREFERENCE Light Yellow - Mountain Dairy Lemonade Dark Yellow –Xtremo Citrico Vibrante Gatorade Chartreuse – 350 mls. Lemon Lime Gatorade plus 150 mls. Green Squall Powerade Dark Chartreuse – Green Squall Powerade Emerald Green– Watermelon Gatorade EXPERIMENT B - PAIRED COMPARISON TEST 635T1 = 0% 573T2 = 1% EXPERIMENT C - TRIANGLE TEST 777Cl = 0% 542E2 = 0% 112H9 =1% EXPERIMENT D - RANKING 495P2 = 0% 543K8 = 1% 695F8 = 2.5% 192L3 = 5% 555D7 =10% EXPERIMENT 2 - DUO-TRIO 8175 (Standard) = Nabisco Nilla Wafers 6104 = Safeway Vanilla Wafers 1108 = Nabisco Nilla Wafers EXPERIMENT 3 - SCORING 420M = 1% 0110 = 2.5% S723 = 5%  

                   

  •   32  

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