Pereskia bleo

5/22/2018 Pereskia bleo

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Introduction of Pereksia bleoOrigin: South America

(Columbia) & Mesoamerica(Panama)

Distribution: South andCentral America, Brazil,Mexico, India and Malaysia.

Common names: Rose cactus,

Chinese rose & Jarum TujuhBilah in Malay.

(Griffith, 2013) &

(Zareisedehizadeh, 2014)


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Pereskia bleo

Habitat: Tropical and Subtropical

2-8 meters height

Areole with spines when young and

naked while growing.

Flowers in bright red or orange-red

with 4-6 cm in diameter.

Fruits are yellow when matured

with 4-5 cm in diameter.

(Anderson, 2010)


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The usage of Pereskia bleoTraditional Usage :Crushed Leaves Clarify water

Eaten Raw or brewed detoxification

Hypertension, gastric pain, ulcer & headache

Modern Usage:

Cytotoxicity activity toward cancer cell lines:

1. Human breast carcinoma

2. Human nasopharyngeal epidermoid carcinoma

Antimicrobial activity in methanol extract.

(Khor, 2013) & (Zareisedehizadeh, 2014)


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Objectives To establish the suitable surface sterilisation protocols

for the leaf and stem explants of P. bleo

To study the effects of different type of auxin andcytokinin on callus indction of P. bleo

To study the leaf or stem of P. bleowhich is moresuitable for callus induction of P. bleo.

To study the light and dark incubation condition forcallus induction of P. bleo.


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Literature Review


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Plant Tissue CulturePlant Tissue Culture:

A technique that grows the aseptic plant tissue to cells,

tissues and organs on the nutrient medium undercontrolled conditions.

Callus Induction:

A process that proliferation of meristematic andundifferentiated cells from wounded region of anexplant (tissue or organs)in response to injury.

(Smith, 2013)


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Plant Growth RegulatorsAn organic compound, either natural or synthetic, that

modifies or controls one or more specific physiologicalprocesses within a plant to accelerate or retard the rate

of growth or maturation, or otherwise alter thebehavior of plants or their produce.

Types of PGR: Auxin and Cytokinins.

(Fishel, 2012)


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Methodology


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Overview of Experimental Design

Surface

Sterilization

Auxin

&

Cytokinin

Incubation

LightCondition

Dark

Condition

Transfer

(Young Leaves)

(Young Stems)

3 Replicates


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Murashige & Skoog

Medium PreparationMacronutrient - NH4NO3, KNO3, CaCl22H2O, MgSO47H2O , KH2PO4,

FeSO47H2O , Na-EDTA 2H2O

Micronutrient - H3BO3 , MnSO4 7H2O, ZnSO4 , KI , NaMoO4 , CoCl2 , CuSO4

Sugars - Sucrose.

Vitamins - Glycine, myo-Inositiol, Thiamine HCL.

Solidifying Agent - Agar powder.

(Maluszynski, 2003) & (George , 2008)


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Surface Sterilization:

1. Wash explant with warm soapy water and rinse in tapwater.

2. A brief of 35% alcohol rinse (for waxy leaf) followed

by washing with 10% Clorox solution with 2 drops ofTween-20.

3. Rinse with sterile water for 3 to 5 times in laminar airhood.

(Chuah 2007 ) & (Smith, 2013)


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Transfer explants to

culture medium

MS medium+

Auxin1.IAA - Natural

2.NAA

3.2,4-D

4.Dicamba

5.Picloram

Synthetic

Cytokinin1.BAP

2.KinetinSynthetic

Each types of

PGR culturefor triplicate


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Incubation

21 replicate with PGR & 3 replicate without PGR

(Raad M.K. et al, 2012)

1. Light condition(16 hours light + 8 hours dark)

2. Dark Condition

(24 hours dark)

28 degree Celsius

Observation(weekly)

& record data


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Expected Results To obtain suitable surface sterilisation protocols for the

leaf and stem explants of P. bleo

To understand the effects of different type of auxin andcytokinin on callus indction of P. bleo

To know the leaf or stem of P. bleowhich is moresuitable for callus induction of P. bleo.

To identify light or dark incubation condition suitablefor callus induction of P. bleo.


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References Anderson E.F. (2001), The Cactus Family, Oregon : Timber

Press, inc.

Griffith P., Gann G.D. & Nassar, J. (2013). Pereskia bleo. TheIUCN Red List of Threatened Species. Version 2014.1.

Available from< http://www.iucnredlist.org/details/152000/0 > ,[Accessedon 5th June 2014].

Chuah E.L. & Chan L.K. (2007), Induction of somatic

embryogenic callus from the leaves of Pereskia grandifolia,Biotechnology 6(1) : 45-48 p. Available from,[Accessed on 19 June 2014]


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References Edwards E.J. & Donoghue M.J. (2006), Pereskia and the

origin of the cactus life form, The american naturalist ,Vol 167 (6) , Available from [Accessed on5thJuly 2014]

Rubluo A. et al., (2002), Auxin induced morphogeneticresponses in long-term in vitro subcultured Mammillaria

san-angelensis Sanchez-Mejorada (Cactaceae), ScientiaHorticulturae 95, 341-349 p.

Smith R.H., (2013), Plant Tissue Culture: Techniques andExperiments, 3rdedn, Academic Press, UK.


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References Raad M.K. et al, (2012), Callus induction and organogenesis

capacity from lamina and petiole explants ofAnthuriumandreanumLinden (Casino and Antadra), Australian

Journal of Crop Science, Vol 6 (5), p. 928-937, Availablefrom[Accessed on 19thJune 2014]

Khor P.Y. et al. (2013), A preliminary survey on themedicinal uses and effectiveness of pereskia bleo used by

people of three villages in the state of kelantan, Malaysia,International journal of herbal medicine 2013, Vol 1 (3), 1-4p. , Available from< http://florajournal.com/vol1issue3/aug2013/2.1.pdf >

[Accessed on 5th July 2014]


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References: Mwenebanda B.M.L., (2002), Potential use of Dormant

Buds as Explants and Callus Culture for Micropropagationof Ginger (Zingiber offocinale Roscoe), Available from [26th

June 2014] Zareisedehizadeh S., Tan C.H., Koh H.L., (2014), A Review

of Botanical Characteristics, Traditional Usage, ChemicalComponents, Pharmacological Activities and Safety ofPereskia bleo (Kunth) DC, Available from[5thJune 2014]

Fishel F.M. (2012), Plant Growth Regulators, Available from[Accessed on 5thJuly 2014]


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References: George E.F. et al. (2008), Plant propagation by tissue

culture, 3rdedn, 65-113 p, Available from [Accessed on 5thJuly 2014]

Maluszynski et al. (2003), Double haploid production incrop plants, 405-413 p., Available from [Accessed on 5thJuly 2014]


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Appendix


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Appendix


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Thank You!

Pereskia bleo

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