parasitology lab notes
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Transcript of parasitology lab notes
![Page 1: parasitology lab notes](https://reader036.fdocuments.net/reader036/viewer/2022062307/555085d9b4c905a85c8b496d/html5/thumbnails/1.jpg)
Lab 4: Cestodes
The Tapeworms!
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General anatomy of a tapeworm
• Tapeworms are long, segmented, flattened & ribbonlike
• On extreme anterior end is the scolex or the head. This is the holdfast organelle.
• The scolex has 4 suckers called acetabula
• Some tapeworms are armed with rostellum, backward-facing hooks that help anchor the worm in the small intestine.
• Posterior to scolex is the strobila or the neck – this is where the proglottids, or segments, are formed.
• The proglottids closest to the scolex are immature, those farthest are gravid - the oldest.
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Tapeworm Reproduction• Tapeworms are hermaphroditic, the
proglottids contain both male & female sex organs.
• Cross-fertilization & self-fertilization occur between/within individual proglottids
• Gravid proglottids are “spent” and contain only a uterus filled with egg packets. They may break off and exit via feces or around anus. Can be seen with the naked eye.
• Proglottids rupture in external environment & release thousands of hexacanth embryos.
• Hexacanth embyros have six hooks.
Better picture on page 72 of textbook
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Dipylidium canninum: “cucumber seed tapeworm”
• Most common tapeworm of both dogs & cats because the intermediate host is the flea
• Fleas often contain the infective metacestode (larval) stage: cysticercoid
• Scolex is armed & has prominent proboscis covered w/ hooks
• Terminal proglottids are motile and look like moving cucumber seeds on the stool or around anus
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Dipylidium canninum: “cucumber seed tapeworm”
(cont’d)• Terminal proglottids
contain thousands of egg packets, each containing 20-30 hexacanth embryos
• Prepatent period is 14-21 days
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Dipylidium canninum: Life cycle
Dog, cat becomes infectedby ingesting fleas thatare infected with cysticercoid larval stage
Also important to note thatthese are ZOONOTIC!
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Dipylidium canninum: Control
• Flea control is the number one way to prevent tapeworm infection
• Treatment is usually with a single dose of praziquantel (Droncit)
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Taenia spp.
• 2nd most common type of tapeworm seen in clinics
• Segments are more rectangular shaped
• Also zoonotic
• Intermediate hosts are rodents & rabbits
• Seen more frequently in cats than in dogs
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Taenia spp.: Anatomy
• Scolex is armed with 2 rows of hooks or acetabula
• Also manifests with motile terminal proglottid segments that may appear on feces or around anus
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Taenia spp.
• Terminal proglottids contain eggs with hexacanth embryos (not egg packets!)
• Striated eggshell, slightly oval shaped & contain single oncosphere with three pairs of hooks
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Taenia spp.: Life Cycle
• Metacestode (larval) stageis cysticercus: bladder wormfound in intermediate host
•Embryos develop into cysticercus in peritoneal cavity of intermediate host •One cysticercus=one adulttapeworm
•More pathogenic to intermediate host than to definitive host
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Echinococcus granulosis &E. multilocularis:
Hydatid disease tapeworms• E. granulosis = dogs, encysts in
viscera & brain
• E. multilocularis = cats, encysts in lungs & alveoli
• Has only 3 proglottids, very small
• Eggs are very similar to Taenia spp. – adult must be identified
• EXTREME ZOONOTICPOTENTIAL!!!!!
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Echinococcus granulosis &E. multilocularis
• Hydatid cysts result in thousands of scolex
• Size is impingent on organs• Damages area where it
resides (brain, liver, lung)• Neoplastic lesions can
grow as large as a human head
• Can kill host if burst open, causes anaphylaxis
• Wild herbivores are intermediate hosts
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Anoplocephala spp.: Equine tapeworms
• Most common equine tapeworm
• Lives in small & large intestine, occasionally in stomach
• Proglottids are wider than they are long
• Intermediate host is grain mite & occasionally beetles
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Monezia spp.• Infect intestinal tract of cattle,
sheep & goats
• Scolex is unarmed
• Proglottids are very short & wide
• Eggs are square or triangular shaped, possess a pyriform apparatus (pear-shaped)
• Intermediate host is oribatid grain mites
• Can cause GI stasis in calves
• Can cause ulcerative lesions where attached
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Diphyllobothrium latum: Broadfish tapeworm
• Pseudotapeworm w/ zoonotic potential
• Continually release eggs until exhausts uterine contents
• Terminal proglottids become senile rather than gravid and detach in chains
• Eggs are light brown, oval & possesses single operculum at one pole
• Eggs are unembryonated when passed in feces
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Diphyllobothrium latum
• Tapeworm of fish-eating mammals
• Has 2 intermediate hosts, copepod & fish
• Encysts in fish muscle• Larvae develop on
intestinal wall
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Identify!
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Identify!
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Identify!