GQBBGQBBGRUP DE QUÍMICA BIOLÒGICA I BIOTECNOLOGIA

Founded in 1998. Consolidated group by Generalitat de Catalunya since 1999g y y

GQBB staff

Dr. Eduard Barberà

Dr. Antoni PlanasHead of the Bioengineering

Departament Dr. Eduard BarberàProf. CatedràticLab. BIOTECHNOLOGY

DepartamentProf. CatedràticLab. BIOCHEMISTRYGQBB, Coordinator

Dra. Magda FaijesProf. TitularLab BIOCHEMISTRYLab. BIOCHEMISTRY

Dr Xavier Turon

Dra. Carme BrosaProf. Catedràtic

Dr. Xavier TuronProf. AssociatBIOPROCESS Unit

Lab. STEROIDSGroup: GQBB

Dr. Xevi BiarnésProf. Contactat

LaboratoryProf. ContactatBIOINFORMATICS Unit GLYCOCONJUGATES

IQAC-CSIC Dr. Gregori ValenciaDra. Gemma Arsequell

GQBB

OBJECTIVE: molecular approaches to biological processes and applications in biomedicine, industrial biotechnology, and food technology

TARGETSBIOMEDICINE: infectious diseases (mycoplasmas, tuberculosis)

neurodegenerative diseases

Methodologies:Molecular biology Protein engineering

BIOTECHNOLOGY:biocatalysis for biomaterials and fine chemicalsfermentation processes for high-value products

BioinformaticsBioprocessesOrganic Synthesis

fermentation processes for high value products

FOOD TECHNOLOGY:functional oligosaccharides Process Controlfunctional oligosaccharidespolyunsaturated fatty acids

AGRONOMICS: plant growth regulators

From GENE to PRODUCT

AGRONOMICS: plant growth regulators

GQBBBIOMEDICINE

- Mycoplasma genomics: novel GTs in membrane glycolipid biosynthesis astherapeutic targets against Mycoplasma infections

Triglycerides

Glycolipid (DPA)

OHO

OHHO

OH

O

OO R

O

OHO

OHO R

OO R

O

R

β-1,6

M. genitalium

PE (Nihydrin)PC (Draggendorff)

y p ( )

Glycolipid (DPA)

OHOHO O O R

OOH

OHOHO O O R

OOHOH

O R

O

mg517: glycosyldiacylglycerol synthase

- Neurodegenerative diseases: protein misfolding and amyloidogenesis

E. Andrés, N. Martínez, A. Planas.. J. Biol. Chem. 286, 35367–35379 (2011)

- Neurodegenerative diseases: protein misfolding and amyloidogenesisHuman transthyretin: design and throughput screening of amyloidosis inhibitors

F

F

COOH

OHI

I di ti h th iIodination hypothesisT. Mairal, J. Nieto, et al. Iodine Atoms: A New Molecular Feature for the Design of Potent Transthyretin Fibrillogenesis Inhibitors. PLoS ONE 4(1): e4124 (2009)

GQBBBIOCATALYSIS

- Glycosynthases: engineered glycosidases for oligosaccharide synthesisDesign and applications to the enzymatic synthesis of carbohydrate analogsDesign and applications to the enzymatic synthesis of carbohydrate analogs

OO

O R2OOH

O OOH

HOOH

OHO

HO

HO

OO

O R2OOH

O OOH

HOOH

OHO

HO

HO

Plant cell wall biosynthesis. Xyloglucan oligosaccharides for XET enzymes

OOHO

OHO O

O

R2O O OHO

OHOO

HOO

OOH

OH

OH OHO

HO

HO

OH

n

OHO

OHO O

O

2 OHO

OHOO

HO

OOH

OH

OH OHO

HO

HO

OH

Saura, Fauré, Piens, Brumer, Teeri, Cottaz, Driguez, Planas, Biochem. J. 395, 99 (2006)F é S B Pl C tt D i J O Ch 71 5151(2006) Fauré, Saura, Brumer, Planas, Cottaz, Driguez J. Org. Chem. 71, 5151(2006) Saura, Fauré, Brumer, Teeri, Cottaz, Driguez Planas. J.. Biol. Chem. 283: 21853 (2008)

Biomaterials: Polysaccharide microstructures

n

OH

OH

HO OHO

OHO

OHO

OH

O

OH

HOOH

OO OH

O

OH

OH

HO

OH

OH

HO OHO

F

OHO

OHO

OH

Biomaterials: Polysaccharide microstructures

glycosynthase

Faijes, Imai, Bulone, Planas, Biochem.J. 380, 635 (2004)Pérez, Faijes, Planas, Biomacromolecules, 12, 494–501 (2011)

GQBBBIOINFORMATICS

- Protein structure modeling and conformational motions by metadynamics:motions by metadynamics: glycosyltransferases as therapeutic targets

Structural model proposed for MG517 obtained after four independent MD simulations of 1 µs each. Black arrow indicates the helix converged in the

variable zone (gray).

GQBBBIOPROCESS

RESEARCH TOPICS- Recombinant protein and metabolite expression, bioprocess

d bi t ti i tiand bioreactors optimization.

- Industrial byproducts as a source for highly valuable biotechnological productsg p

- Downstream processing and bioproducts isolation.

- Heterotrophic microalgae: biotechnological production of polyunsaturated fatty acids ω-3 fatty acids: DHA and EPA

Sergi Abad, Xavier Turon. Valorization of biodiesel derived glycerol as a carbon source to obtain added-value metabolites: Focus on polyunsaturated fatty acids. Biotechnology Advances, 30 (3), 733-741 (2012)

GQBBFOOD TECHNOLOGY

- HUMAN MILK OLIGOSACCHARIDES: engineering a glycosynthase for the production of Type-1 HMOengineering a glycosynthase for the production of Type 1 HMO

Lacto-N-tetraosa (Tipo I)( )

HMO: nutrients for intestinal microfloraand immunostimulants in infants

Target enzyme:Target enzyme:Lacto-N-biosidase from B.bifidum

GQBBAGRONOMICS

- Plant growth regulators: synthesis and biological activity of brassinosteroids

OH

OH

HO

HO O

OH

OH

Synthesis of brassinosteroid analogs- Synthesis of brassinosteroid analogs- Biological activity on plant growth- Structure-activity relationships

Goal: novel fitochemicals for improved crop yields and resistance to stress and disease

GQBBCURRENT FUNDED PROJECTS

- CHITOBIOENGINEERING: european project 2011-2013- MYCOPAT: Plan Nacional de Biología Fundamental. MICINN 2011-2013MYCOPAT: Plan Nacional de Biología Fundamental. MICINN 2011 2013- CAP MARATÓ: Fundació Marató TV3 (enfermedades cardiovasculares) 2010-2012- MARATÓ PROTEOMICS: Fundació Marató TV3 (enfermedades raras) 2011-2013- HALOFARMA: Explora project MICINN 2011-2012- STARS: european Marie Curie training network 2011-2013- TRANSGLUTAMINASES: Plan Nacional de Biología Fundamental MICINN 2011-2013TRANSGLUTAMINASES: Plan Nacional de Biología Fundamental. MICINN 2011 2013