P-6-20 Erythrocyte sorbitol levels fluctuate rapidly in response to changes in ambient glucose...
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P-6-20 ERYTHROCYTE SORBITOL LEVELS FLUCTUATE RAPIDLY IN RESPONSE TO CHANGES IN AMBIENT GLUCOSE CONCENTRATION. Y.Nagasaka, S.Fujii, Y.Yoshizaki and T.Kaneko The Third Department of Internal Medicine, Yamaguchi University School of medicine, Ube, Japan Clinical application of measuring erythrocyte sorbitol as an index of diabetic control is still controversial. In the present study, eryth- rocytes from diabetic and non-diabetic subjects were incubated with various glucose concentration (5-50 mM) to investigate how fast sorbitol was built up and how fast the once built up sorbitol was oxidized. The results indicated that erythrocyte sorbitol levels increased rapidly in response to increased glucose concentration and the once built up sorbitol easily decreased according to the rapid reduction of glucose concentra- tion. This was further supported by the results of 75gOGTT in a diabetic and a non-diabetic with gastrectomy, who showed marked early hyperglycemia caused by rapid absorption of ingested glucose and subsequent rapid reduc- tion of plasma glucose concentration. In conclusion, assessing the long or medium term diabetic control by measuring erythrocyte sorbitol appears not to be reasonable because of the rapid fluctuation of sorbitol respon- sive to sudden changes in glucose concentration. P-6-21 Relation between Amino Acids, Metabolism and Blood Viscosity, Platelet Aggregation, PGI2, TXB2, Triglyceride in IDDM Wang Zhi-li, Liu Zhe, Yin Wei, Yu De-min Department of Endocrinology, Hospital attached to Tianjin Medical College, Anshan Road, Tianjin, People's Republic of China In this study the blood concentration of amino acids (Isoleu, Leu, Val, Lys, Phe, Thr, Met, Arg, His, Asp, Glu, Pro, Gly, Ala, Cys, Ser, Tyr) C-P, glucagon, TXB2, PGI2 and blood viscosity, platelet aggregation were measured in 25 Type I diabetic patients and 26 controls. The results showed that islet B cell had little secretion (C-P 0.06 ~ 0.04 pmol/ml), the secretion of glu- cagon (198.4 ± 34.5 pg/ml) was more than controls (98.4 ± 19.3 pg/ml). The blood levels of branched chain amino acids (Leu, Isoleu, Val) and Phe, Ala, Gly, Dru, Lys were increased significantly compared to controls (P<O.OI). The concentration of Thr, Met, Arh, Ala, Pro were reduced (P<O.OI). The levels of TXB2 and the ratio of TXB2/PGI2 was higher than controls (P~O.OI). The bloo~ viscosity and platelet aggregation was also higher than controls (P<O.I). Significant correlations were observed between the relation of TXB2/PGI2 and platelet aggregation, the levels of total BCAA (Val, Leu, Isoleu) and the size under cure of glucose. But no significant correlations were observed between the ratio of TXB2/PGI2 and the levels of total BCAA, and between levels of C-P and total BCAA. These results suggested that: (i) there is an imbalance in TXB2/PGI2 in IDDM, and (2) that BCAA was higher in IDDM than controls was due to hyperglycemia. S58
Transcript of P-6-20 Erythrocyte sorbitol levels fluctuate rapidly in response to changes in ambient glucose...
P-6-20 ERYTHROCYTE SORBITOL LEVELS FLUCTUATE RAPIDLY IN RESPONSE TO CHANGES IN AMBIENT GLUCOSE CONCENTRATION. Y.Nagasaka, S.Fujii, Y.Yoshizaki and T.Kaneko The Third Department of Internal Medicine, Yamaguchi University School of medicine, Ube, Japan
Clinical application of measuring erythrocyte sorbitol as an index of diabetic control is still controversial. In the present study, eryth- rocytes from diabetic and non-diabetic subjects were incubated with various glucose concentration (5-50 mM) to investigate how fast sorbitol was built up and how fast the once built up sorbitol was oxidized. The results indicated that erythrocyte sorbitol levels increased rapidly in response to increased glucose concentration and the once built up sorbitol easily decreased according to the rapid reduction of glucose concentra- tion. This was further supported by the results of 75gOGTT in a diabetic and a non-diabetic with gastrectomy, who showed marked early hyperglycemia caused by rapid absorption of ingested glucose and subsequent rapid reduc- tion of plasma glucose concentration. In conclusion, assessing the long or medium term diabetic control by measuring erythrocyte sorbitol appears not to be reasonable because of the rapid fluctuation of sorbitol respon- sive to sudden changes in glucose concentration.
P-6-21
Relation between Amino Acids, Metabolism and Blood Viscosity, Platelet Aggregation, PGI2, TXB2, Triglyceride in IDDM Wang Zhi-li, Liu Zhe, Yin Wei, Yu De-min Department of Endocrinology, Hospital attached to Tianjin Medical College, Anshan Road, Tianjin, People's Republic of China
In this study the blood concentration of amino acids (Isoleu, Leu, Val, Lys, Phe, Thr, Met, Arg, His, Asp, Glu, Pro, Gly, Ala, Cys, Ser, Tyr) C-P, glucagon, TXB2, PGI2 and blood viscosity, platelet aggregation were measured in 25 Type I diabetic patients and 26 controls. The results showed that islet B cell had little secretion (C-P 0.06 ~ 0.04 pmol/ml), the secretion of glu- cagon (198.4 ± 34.5 pg/ml) was more than controls (98.4 ± 19.3 pg/ml). The blood levels of branched chain amino acids (Leu, Isoleu, Val) and Phe, Ala, Gly, Dru, Lys were increased significantly compared to controls (P<O.OI). The concentration of Thr, Met, Arh, Ala, Pro were reduced (P<O.OI). The levels of TXB2 and the ratio of TXB2/PGI2 was higher than controls (P~O.OI). The bloo~ viscosity and platelet aggregation was also higher than controls (P<O.I). Significant correlations were observed between the relation of TXB2/PGI2 and platelet aggregation, the levels of total BCAA (Val, Leu, Isoleu) and the size under cure of glucose. But no significant correlations were observed between the ratio of TXB2/PGI2 and the levels of total BCAA, and between levels of C-P and total BCAA. These results suggested that: (i) there is an imbalance in TXB2/PGI2 in IDDM, and (2) that BCAA was higher in IDDM than controls was due to hyperglycemia.
S58
