Oxygen and Carbon Dioxide transport in the blood
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Transcript of Oxygen and Carbon Dioxide transport in the blood
Oxygen and Carbon Dioxide transport in the
blood
Majority of O2 and CO2 is transported in the blood by:
O2 combing with hemoglobinCO2 transformed into
bicarbonate (HCO3¯)
Hemoglobin and O2 transport±99% of O2 transported in blood
is chemically bound to hemoglobin
Hemoglobin is a protein found in red blood cells (erythrocytes)
Each hemoglobin molecule can hold up to 4 O2 molecules
O2 combined with hemoglobin = oxyhemoglobin
O2 that is NOT combined with hemoglobin = deoxyhemoglobin
Oxyhemoglobin
The amount of O2 that can be transported depends on the concentration of hemoglobin
Normal, healthy men = 150g/L of blood
Normal, healthy women = 130g/L of blood
When a hemoglobin molecule is completely saturated with O2 it can transport 1.34ml of O2
Therefore if hemoglobin if full (100% saturated with O2):
Healthy male can transport 200ml of O2
Healthy female can transport 174ml of O2
100% saturation occurs at sea level (low altitude)
Partial PressureThe amount of O2 bound to hemoglobin is directly
related to the partial pressure of O2.
PO2:All gases exert pressure on the walls of their container because the molecules of gas bounce off the walls.
Partial pressure is used to describe a mixture of gases.
Defined as the pressure that any one gas would exert on the walls of the container if it were the only gas present
In the lungs, where the alveoli and capillaries exchange gases, PO2 is high
Therefore O2 binds instantly to hemoglobin.
As the blood circulates to other body tissue the PO2 becomes lower
Therefore hemoglobin releases O2 into the tissue because the hemoglobin cant maintain its full capacity of O2
Oxyhemoglobin Dissociation Curve
In the alveolar capillaries in the lungs:
O2 binding with hemoglobin is called loading
Release of O2 from hemoglobin is called unloading
Loading & unloading are reversible actions: Deoxyhemoglobin + O2 Oxyhemoglobin
Oxyhemoglobin Dissociation Curve An important tool for understanding how our blood
carries and releases oxygen.
Relates oxygen saturation (SO2) and partial pressure of oxygen in the blood (PO2)
Determined by what is called "hemoglobin's affinity for oxygen“
how readily hemoglobin acquires and releases oxygen molecules from its surrounding tissue.
Oxygen hemoglobin dissociation curve
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Oxygen Dissociation Curve
Shows the percentage of O2 that is bound to hemoglobin at each O2 pressure.
The curve is S-shaped with a steep slope between 10 and 60 mmHg and a flat portion between 70 and 100 mmHg.
At rest the body’s O2 requirement is low & only ±25% is unloaded into muscles
At intense exercise, PO2 can reach 20mmHg and 90% of O2 is unloaded into muscles
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Significance of the Flat Portion The flat portion of the curve shows that the PO2 can fall from
100 to 60 mmHg and the hemoglobin will still be 90% saturated with O2
At pressures above 60mm Hg, the dissociation curve is relatively flat.
This means the O2 content does not change much (even with large changes in the partial pressure of oxygen)
E.g. PO2 can fluctuate between 90 – 100mmHg without a large drop in the percentage of hemoglobin that is saturated with O2
*This is important because there is a drop in PO2 with aging and with climbing high altitudes
Haemoglobin Saturation at High Values
Lungs at sea level: PO2 of 100mmHg haemoglobin is 98% SATURATED
Lungs at high elevations: PO2 of 80mmHg, haemoglobin 95% saturated
Even though PO2 differs by 20 mmHg there is almost no difference in haemoglobin saturation.
When the PO2 in the lungs declines below typical sea level values, haemoglobin still has a high affinity for O2 and remains almost fully saturated.
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Significance of Steep PortionPO2 reductions below 40 mm Hg produce a rapid
decrease in the amount of O2 bound to hemoglobin.
When the PO2 falls below 40 mm Hg, the quantity of O2 delivered to the tissue cells may be significantly reduced.
As PO2decrease in this steep area of the curve, the O2 is unloaded to peripheral tissue as hemoglobin’s affinity for O2 diminishes.
Therefore, small changes in PO2 will release large amounts of O2 from hemoglobin.
* This is critical during exercise when O2 consumption is high.
Haemoglobin Saturation at Low Values
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Factors that affect the O2 Dissociation
◦pH - Change in the blood pH◦Temperature- temp increases = the
curve moves to the right◦Carbon Dioxide – increased PCO2
Factors Altering Haemoglobin Saturation
Factors Altering Haemoglobin Saturation (Exercise)
ExampleDuring exercise, the
oxyhemoglobin dissociation curve will shift to the RIGHT
This is because the pH in the body is decreased (from increased lactic acid) AND
Temperature increases during exercise
O2 Transport in muscleMyoglobin:
- Protein that binds with O2
- Found in Skeletal and Cardiac muscle fibers (not in blood)
- Acts as a shuttle to transport O2 from muscle cell membrane to the mitochondria
- Found in large quantities in slow-twitch fibers (high aerobic capacity)
- Smaller amounts in intermediate fibers - Limited quantity in fast twitch fibers
Myoglobin has a similar structure to hemoglobin, but is ¼ weight
Difference in structure = difference in affinity for O2
Myoglobin has a greater affinity for O2: Therefore the myoglobin-O2 dissociation curve is much
steeper
= myoglobin releases O2 at very low PO2 values
NB because PO2 in mitochondria of contracting skeletal muscle can be as low as 1mmHg.
Myoglobin stores O2 = reserve O2 for transition from rest to exercise
At the start of exercise there is a lag time from the onset of muscular contraction and increased O2 delivery to the muscles
Therefore O2 bound to myoglobin before exercise acts as a buffer
so that muscles can receive O2 until the cardiopulmonary system can meet the new O2 demand
At the end of exercise:- myoglobin- O2 stores must be replenished to ensure O2 is available for the next time exercise begins
Therefore O2 consumption above rest contributes to the O2 debt
i.e. O2 consumption continues after exercise has stopped leading to an O2 debt (O2 deficit)
(Anaerobic metabolism of lactate – also called EPOC Post Exercise Oxygen Consumption)
Carbon Dioxide Transport in BloodCO2 transported in the blood by:
1. Dissolved CO2 (±10%)2. CO2 bound to hemoglobin (±20%)3. Bicarbonate (HCO3¯)(±70%)
Dissolvedbound to HbHCO3-
CO2 is converted to bicarbonate in red blood cells:
A high PCO2 causes CO2 to combine with water, forming carbonic acid.
This reaction is rapidly catalyzed by the enzyme Carbonic Anhydrase
The carbonic acid then dissociates into bicarbonate ion and hydrogen ion.
The hydrogen ion then binds with hemoglobin
The bicarbonate ion diffuses out of the red blood cell into the blood plasma
Transport of CO2Carbon Dioxide Transport
H2 CO3 CO2 + H2O
H2 CO3 H+ + H-CO3
CA
Carbonic Acid
Bicarbonate ion
Bicarbonate Ion ExchangeBecause bicarbonate carries a negative charge
(anion), removal of a negatively charged molecule from a cell = electrochemical imbalance
Therefore the negative charge must be replaced
Bicarbonate is replaced by chloride (Cl¯) which diffuses from the plasma into the red blood cell
This is called chloride shift the shift of anions into red blood cells as blood moves through tissue capillaries
When blood reaches the pulmonary capillaries:
PCO2 of the blood is greater than that of the alveolus = CO2 diffuses out of the blood across the blood-gas interface
At the lungs:
Binding of O2 to hemoglobin causes a release of the hydrogen ions (which are bound to hemoglobin) to promote the formation of carbonic acid
H+ + HCO3¯ H2CO3
In conditions where PCO2 is low (at the alveolus), carbonic acid then dissociates into CO2 and H2O
H2CO3 CO2 +H2O
The release of CO2 from the blood into the alveoli is removed from the body in expired gas (CO2 we breathe out)
Revision Questions1. What is the amount of hemoglobin found in a
normal male and female? (2)
2. What is the amount of oxygen carried in a normal male and female? (2)
3. What is partial pressure? (2)
4. Explain the oxghemoglobin dissociation curve, focusing on the use, flat and steep portions (10)
5. What factors affect the oxghemoglobin dissociation curve? (3)
Revision Questions 6. How does exercise affect the oxghemoglobin
dissociation curve? (3)
7. What is myoglobin and where is it found? (4)
8. How is myoglobin different to hemoglobin? (4)
9. What are the differences in myoglobin and oxygen at the start and end of exercise? Why does this happen? (8)
10. What are the way in which carbon dioxide is transported? (3)
11. How is carbon dioxide converted to bicarbonate? (5) 12. Explain bicarbonate exchange. (8)