OSAP Invertebrates 2010

8/11/2019 OSAP Invertebrates 2010

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Ontario Stream Assessment Protocol

(OSAP)

South Nation Conservation

Toronto and Region Conservation Authority

Ontario Ministry of Natural Resources

Department of Fisheries and Oceans


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Section 2:

Benthic Macroinvertebrate

Assessment


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Objective

Provide a assessment techniques for benthic

invertebrates as an indicator of a potential water

quality impairment at the site level

Techniques vary with effort and diagnostic

capabilities

link to EPA, MOE, OBBN, Watershed Report Card

attempt to standardize approaches used

8/31/2014 Ontario Stream Assessment Protocol


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Steps

1. Determine Study Design2. Use Section 1 to identify and document site

boundariesuse S1 M5 (New) Site Features for

Water Quality Surveys to maintain consistency with

OBBN Includes noting dominant substrate types, aquatic

macrophytes present, bankfull width, canopy cover and river

characterization

3. Select sample locations

4. Document habitat conditions

5. Collect sample

6. Process sample



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Study Design Considerations

precision of data collected

type of study

resources

Questions influence, method used, type of processing,

Taxonomy level, preservation, identification location



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Sampling Techniques

Rapid Assessment

10 large particles or kick smaller substrates into net,determine taxa presence and relative abundance

Stationary Kick Technique

2 - 1m2 samples in riffles, with kick net (2 minutes)

Transect Travelling Kick and Sweep

approx 10 m transect, 3 minutes with d-net

sample area varies with productivity and stream width ,2 riffles and 1 pool



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Document Habitat Conditions:

wetted width of stream (0.1 m)

depth, hydraulic head (nearest 5 mm)

10 substrate particles (median axis diameter in mm)



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Maximum difference (over 3-5 seconds) in the height of water

between the front and back of a ruler when placed at right angles to

direction of flow



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randomly sample and

measure median

diameter of 10 substrate

particles



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Transect Kick & Sweep sample a minimum of 10 linear metres in approximately

3 minutes along each collection area (transect)

generally provides 100 animals or more

if stream width is less than 10 m, multiple transects

should be sampled such that the total distance sampledis at least 10 m (i.e., a distance greater than 10 m maybe attained as transects must be sampled in theirentirety)

if the stream width is greater than 10 m, the entire widthmust be sampled

transects are established perpendicular to the mainconcentration of flow (i.e., thalweg)



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Traveling Kick and Sweep-

Transect Method (ForWadeable or Partially

Wadeable Stream Reach)

Flow

Transect

Sampling

Location

Sampling Reach

Boundary



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Steps: Stationary Kick

Place net (window screen on sticks) into water at 70degree incline



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kick streambed for 1 minute over an area of approx. 1 m2

rub rocks in sample area for 1 additional minute



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two people lift net from water, scrape into bucket



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The study design will dictatewhich of the following options

are selected:

preserved or live

laboratory or field picking

Marchant box or spoon sub-sampling

use of a microscope or no use of a microscope taxonomic level desired

specimen archiving or discarding

Sample Processing



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Sample Processing (cont)

Continue to subsample and pick until a

minimum of 100 bugs have been picked

(notemust pick last subsample in its

entirety or no additional bugs are foundduring a 1-3 minute period)

Record amount of sample picked (by

weight or volume)



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Verifying Field Identification

1. Used to ensure correct identification

2. Preserve in 70 % alcohol

3. Identify in lab, record on new form and enter as thesame sample id but you must indicate that it is wasidentified in the lab



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Field Identification

3

4

5

6

7

8

3 4 5 6 7 8

visit 1

CV = 4.46

visit 2

laboratory

Laboratory vs Field Identification

3

4

5

6

7

8

3 4 5 6 7 8

CV = 2.86

Field



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Summary & Interpretation

provides taxa richness from combined sample

comparisons between lab and site

Hilsenhoff index of nutrient enrichment

comparable and robust method for coarse taxonomy

(in fact method differences are not important)



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DESC MNR OBBN TRCA

Sampling Method

Hilsenhoff BI (HBI) - 2004

10

8

6

4

2

0

Methods comparable for both years, similar trend

for other indices

Values increase between years: Year effect

Variances not stat. different: Methods relatively

consistent

DESC MNR TRCA

Sampling Method

Hilsenhoff BI (HBI) - 2003

10

8

6

4

2

0

HilsenhoffBioticInd

ex(HBI)

HilsenhoffBioticInd

ex(HBI)



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Analysis Options

Reference Condition Approach (RCA)

BACI (Before after or upstream/downstream)

Hindcasting (linear regression with deviations from

expected represent impacted sites

http://localhost/var/www/apps/conversion/tmp/scratch_3/OSAP_Tuesday_RCA.ppthttp://localhost/var/www/apps/conversion/tmp/scratch_3/OSAP_Tuesday_RCA.ppt


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Tips

magnifying glasses are a must

do the sample when it is fresh, keep it cold and

shaded learn body movements (of inverts!)

have lots of high quality forceps and eye droppers

handy

question yourself regularly, its easy to get in a bad

habit!



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More Tips

Know the diagnostic characteristics of key bugs

Send samples to Chris Jones for QA/QC

Collect benthos sample before fish sample(electrofishing) if sampling on same day or electrofish

a minimum of 2 weeks prior to collecting benthos



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Next Steps

evaluate influence of water quality vs. habitat on biota

Developing reference conditions and Biocriteria for

South Central OntarioMOE and CA partnership

developing database for lower level taxonomic

identification

certification process for inverts


OSAP Invertebrates 2010

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