OPERATION MANUAL - scopeled.com€¦ · ScopeLED ® does not transfer ownership of software...

SCOPELED® B-SERIES ILLUMINATOR

OPERATION MANUAL

COPYRIGHT © 2017-2018 ScopeLED®

All rights reserved. Printed in the United States of America. This manual may not be reproduced in whole or in part, in any form or by any means, without the express written

permission of ScopeLED®

.

NO LIABILITY FOR ERRORS

ScopeLED®

reserves the right to correct technical and typographical errors in this manual at any time, without prior notice.

In no event shall ScopeLED®

be liable for errors in this manual or for any damages arising out of or relating to this manual.

PRODUCT WARRANTY/LIMITATION OF REMEDITS

ScopeLED®

warrants, to the original Buyer, all of its products to be free from defects in both workmanship and material for

a period of one year from the date of shipment. This warranty extends to all products, which have proved defective through normal use, but excludes products that have been damaged, mishandled, disassembled, modified, or misused by

Buyer or any other person. This warranty is in lieu of all other warranties, and ScopeLED®

disclaims all other warranties

express or implied, including any warranty of merchantability, fitness for a particular purpose, or arising from the course of

dealing between the parties or usage of trade. ScopeLED®

does not extend any warranty of any kind whatsoever to any

purchaser of the products from Buyer or to any end-user of the products. ScopeLED®

, at its sole choosing, will replace or

repair to proper working condition any products under warranty that are returned. Products repaired or replaced under

warranty are only warranted for the remaining unexpired period of time of the original warranty. ScopeLED®

reserves the

right to issue a credit memo for any defective product as an alternative to product replacement or repair. ScopeLED®

will

not accept Buyer generated debit memos. Buyer may not set off or withhold payment because any product is defective. In

no event shall ScopeLED®

liability under this warranty and this contract exceed the purchase price of the products. In no

event shall ScopeLED®

be liable under this warranty or this contract for consequential, incidental or special damages.

RETURN MATERIAL AUTHORIZATION TERMS

ScopeLED will only accept a return of products for which a ScopeLED®

Return Material Authorization ("RMA") Number

has been issued to Buyer prior to the shipment of the return products to ScopeLED®

. This RMA Number must be

displayed on all return shipment documents. ScopeLED®

will refuse all returns that are not accompanied by an RMA

Number. All risks of any such refused shipment are the sole responsibility of Buyer.

For warranty returns, ScopeLED®

will only accept return products accompanied by a statement of defects. ScopeLED®

will not evaluate returns not including this information, and such returns will be returned to Buyer at Buyer's expense. Warranty returns proved defective through damage, mishandling, disassembly, modification, or misuse by Buyer or any other person, and warranty returns found non-defective, will be subject to evaluation and processing fees, and repair costs if applicable. Non-warranty returns will be evaluated and will be subject to evaluation and processing fees. If non-warranty repair work is necessary, Buyer will be notified of repair costs before a repair work order is initiated. Confirming POs are required for non-warranty repair work.

For warranty returns, Buyer is responsible for one-way freight costs to ScopeLED®

, including any duty and taxes.

ScopeLED®

will cover freight costs for return shipment to Buyer. Shipment charges billed to ScopeLED®

without prior

approval from ScopeLED®

will be re-invoiced to Buyer.

For non-warranty returns, Buyer is responsible for two-way freight costs, including any duty and taxes. If shipment consists of returns that are both warranty and non-warranty, the shipment will be considered as non-warranty.

ScopeLED®

will not accept Buyer generated debit memos.

All international return shipments to ScopeLED®

, including packaging and airway bill, must be marked "Goods made in

the United States; enter as American Goods Returned ('AGR')" and state the reason for the return to the United States.

ScopeLED®

will refuse all returns that are not properly documented. All risks of any such refused shipment are the sole

responsibility of Buyer. International returns should be sent via Federal Express, UPS or DHL. International returns may be processed using

ScopeLED®

’s brokerage: EWI Inc. 305 Harbor Way, South San Francisco, CA 94080. Contact David Li at TEL: (650) 794-

1388, FAX: (650) 794-1389. If one of these carriers or ScopeLED®

’s broker is not used, ScopeLED®

may invoice Buyer

for any additional costs including duty and taxes. REVERSE ENGINEERING/CONFIDENTIALITY Buyer shall not reverse engineer, decompile, disassemble, modify, reproduce or copy any products or any software within any products. Buyer shall not analyze or identify the chemical composition or the physical characteristics of any products.

Buyer shall not furnish ScopeLED®

specifications to any other person.

SOFTWARE LICENSE

ScopeLED®

does not transfer ownership of software contained in any products. ScopeLED® grants to Buyer a perpetual

non-exclusive license to use software in the operation of the product in which it is contained. This license is transferable only with the transfer of ownership of the product.


TABLE OF CONTENTS

PRODUCT OVERVIEW ........................................................................................ 1

ScopeLED®

B-Series Illuminator Overview ............................................................................ 1 Models & Naming Format ......................................................................................................... 1 Included Hardware & Accessories .......................................................................................... 3 Safety Instruction ...................................................................................................................... 3

HARDWARE SETUP ............................................................................................ 4 Illuminator Mounting Installation............................................................................................. 4 Installation Recommendations ................................................................................................ 7

MODES OF OPERATION .................................................................................... 9

MANUAL OPERATION ...................................................................................... 11 Manual Controller .................................................................................................................... 11 Basic Operation ....................................................................................................................... 12

SOFTWARE OPERATION via USB (Virtual COM Port) ................................... 13

Virtual COM Port driver Installation ................................................................ 13 Auto installation through Windows Update ......................................................................... 13 Manual installation .................................................................................................................. 14

Serial Commands .............................................................................................. 18 Message Structure .................................................................................................................. 18 USB Virtual COM Port (FTDI FT232RQ) ................................................................................ 19 Commands ............................................................................................................................... 19 Error Codes.............................................................................................................................. 25 Environment Setup ................................................................................................................. 25 Serial Command Examples .................................................................................................... 26 Serial Command Examples using AccessPort Software .................................................... 27 Serial Command Examples using uManager ....................................................................... 32

ScopeLED® B-Series Software for Windows .................................................. 45

EXTERNAL TTL/ANALOG ................................................................................ 51 External Input Connector Overview ...................................................................................... 51 Example of External Trigger Mode Operation ...................................................................... 52

TROUBLE SHOOTING ...................................................................................... 54

SPECIFICATIONS .............................................................................................. 55 Absolute Maximum Ratings ................................................................................................... 55 Optical Specifications ............................................................................................................. 55 Mechanical Dimensions ......................................................................................................... 55 Mechanical Drawings .............................................................................................................. 56


SCOPELED® Page 1 of 56

Copyright © 2016 Product Overview

PRODUCT OVERVIEW This product must be installed in accordance with the applicable installation code by a person familiar with the construction and operation of the product and hazards involved.

ScopeLED® B-Series Illuminator Overview ScopeLED

® B-series offers high brightness, tunable color and adjustable intensity in a compact,

flexible, and convenient package. The advanced thermal design provides a vibration free fan-less cooling with a high intensity optically stable light output. Different colored LED chips packed into DiCon’s Dense Matrix LED™ Array provides high efficiency and ultimate performance to replace conventional halogen light sources used for bright-field, dark-field, phase contrast, polarized light and DIC in transmitted and reflected light applications, illuminating your sample with selectable colors to enhance contrast, protect wavelength sensitive samples, and optimize viewing comfort. The LEDs do not emit in the UV and IR spectral regions, eliminating the need for special filters. Undesirable light and sample interactions such as photobleaching in fluorescence and unintended curing in photosensitive adhesives can be avoided by selecting a color channel that doesn’t interact with the sample. ScopeLED

® B-series also provide versatile operations that can be readily adjustable to most

systems. It can be simply controlled manually or can be automated and synchronized with other hardware via USB or External triggering, making it a great fit for automated production lines and robotics. The RGB model allows any quantitative black and white camera to function as a high-speed color imaging system with separate channel External trigger control, eliminating the need for specific software drivers. Its non-phosphor based DiCon’s Dense Matrix LED™ array is inert to laser excitation allowing it to be used as a self shuttering LED transmitted light source with any confocal or laser based fluorescence microscopy system. The ScopeLED

® B-series combine optical power, flexibility, and usability in a compact form and is

a great choice for your application.

Models & Naming Format The ScopeLED

® B-series has two models

B190

B210 (Focus Lens Version)

Please reference the laser-engraving label at the side of the unit to confirm that you are using one of these models. If not, contact the manufacturer for the proper user manual. Both B190 and B210 illuminators come in single color (5600K) or Mixed RGB output types.




Single Color (B190-5600K or B210-5600K)

The single color illuminator provides single white color at 5600K only. A customized color model may be available upon special request.

Mixed RGB (B190-RGB or B210-RGB)

The mixed RGB illuminator has three individual single-color channels, Red (635nm), Green (521nm) and Blue (465nm), in addition to three CCT tunable whites (3000K, 4300K & 5600K).

Naming Format for ScopeLED®

B-series family product




Included Hardware & Accessories Illuminator x 1

Microscope Mounting Adapter x 1 (See Microscope Mounting Adapters List)

AC/DC Power Adapter x 1

AC Power Cord x 1

B-Series Manual Controller x1

RJ12-6 Cable x1

USB to Mini-USB Cable Cord x 1

Quick Start Guide x 1

Additional microscope mounting adapters (Optional)

Analog to Digital Communication Cable – DE-15P to 2-BNC Male Cable (for single color type) (Optional)

Analog to Digital Communication Cable – DE-15P to 8-BNC Male Cable (for RGB color type) (Optional)

Footswitch (Optional)

Safety Instruction Warnings

Never look directly at the light output port.

Inappropriate usage of ScopeLED® B-Series products could result in eye damage.

Installation of the product onto readily available microscopes should always be performed according to the written guidelines.

Attention (also see Installation Recommendations & Absolute Maximum Ratings)

The statements regarding safety of operation of the B-Series will apply only when the product is operated correctly.

Any installation or attachment of microscope mounting adapter to the B-Series illuminator should always be performed with power adapter and other cables disconnected.

When attaching to or removing from the microscope port, the B-Series illuminator ON/OFF rocker switch should always be set to off.

The B-Series must not be operated in explosion prone environments. The B-Series products must always be operated with shielded cables.



Copyright © 2016 Hardware Setup

HARDWARE SETUP

Illuminator Mounting Installation 1. Turn the Manual Controller ON/OFF Rocker Switch to “OFF” position 2. The illuminator comes with the mounting adapter installed; however, if for any reason the installed microscope adapter needs to be changed, use a 1.5mm allen wrench to loosen the M3 set screws located on the bottom of the front of the illuminator housing. Then take out of the adapter.

3. Place the desired adapter on the illuminator mounting port, and tighten the setscrews to secure the adapter.

4. Attach the ScopeLED B-Series Illuminator with the microscope mounting adapter to the microscope’s transmitted or reflected light lamp-house port. 5. Connect the RJ12-6 cable one end to the illuminator and the other end to the manual controller unit. 6. Connect the AC/DC power adapter to the illuminator at the DC power input port.




7. Plug the AC power cord into the power adapter and then into the wall socket.

NOTE:

The illuminator mounting installation procedure uses B210 model as the illustration. The B190 model mounting installation procedure is the same as shown above.




Illuminators

Manual Controller (Both B190 & B210 share the same manual controller)

1. On/Off switch : To turn on/off Manual Controller. 2. Shutter Enable button : To open/close all shutters of fixture.




Installation Recommendations

Mounting

The ScopeLED

® B-series illuminator is available as B190 unit, or as a B210 unit that is

emphasized on collecting more light. Consider your system and select the unit that works best for your application. The mounting adapter is microscope model dependable. Please follow the table to choose the right adapter to attach on your microscope.

Microscope Mounting Adapters List

Mounting Adapter Name Microscope Model

N3 Nikon transmitted illuminator E600, E800

N4 Nikon transmitted illuminator LV 150, MA, MM, Ti

N5 Nikon epi illuminator IC-66, 80i, 90i, NiU, NiE

N6 Nikon epi illuminator Ephiphot 200/300, TE2000, TE300, Optiphot 200

N7 Nikon Optiphot M adapter/Diaphot, Optiphot 2

N8 Nikon TS100

N9 Nikon Labophot, Labophot2

N10 Nikon E400 Adapter

O3 Olympus BX50, BX61

O4 Olympus BHS

O5 Olympus BX40, BX41

L2 Leica

L4 Leitz Laborlux 12

Z2 Zeiss Illuminator

U1 Unitron metalagraph

AR1 Auxiliary Ring, B to F Series

Environment

The illuminator is not suited for wet or damp locations. Be sure to protect all electronics from moisture. Do not mount or locate the unit in an explosive environment near combustible material.

Air Volume

Do not encase or bury the unit in any way that restricts airflow.

Orientation

The unit is unidirectionally installed to the microscope. Be sure not to install the unit upside down to allow the best work performance.




Power Adapter

The power adapter must be a constant voltage source at 12VDC (±10% tolerance) and can provided at least 2A in order for the Illuminator to function properly. The output connector of the power adapter must be a female barrel connector for direct compatibility with the B-series Illuminator. Though the fitting between the output connector of the power adapter and input connector of the Microscope Illuminator may be snug, the connection will not be able to support the weight of the illuminator. Do not use the power adapter as its own hanging device.



Copyright © 2017 Manual Operation

MODES OF OPERATION There are 3 modes for user to operate B-series illuminator. Please choose the appropriate mode for your application.

1. Manual Operation (Manual Controller) Use two knobs (Color/Intensity) on the manual controller to operate B-series illuminator.

2. Software Operation via USB (Virtual COM Port) Control B-series illuminator on PC via USB cable.

3. External TTL/Analog Signal Source Use external TTL/Analog signal source (both : 0-6 Volt) to control B-series illuminator via ScopeLED® D-Sub to BNC accessory.. (TTL controls Shutter and Analog controls Intensity.)




Priority of Control Modes Shutter and Intensity are independently controlled on the B190/B210. The diagram below shows the priorities of the different modes from top (high priority) to bottom (low priority) Example (Shutter): If the manual controller is powered on while in software operation via USB, the shutter state will be determined by the foot switch. If a TTL 6V is given, then the shutter state will be open. Therefore, when using modes other than external TTL, do not send 6V to the TTL.




MANUAL OPERATION

Manual Controller To manually operate the B-series illuminator, two knobs are available for color tuning (Except on single color models) and intensity levels. The intensity knob is identical for both single color and mixed RGB types of the B-series illuminator. Single Color (B190-5600K or B210-5600K)

Mixed RGB (B190-RGB or B210-RGB)




Preprogrammed Modes

Preprogrammed Modes for Single Color Output Type (B190-5600K or B210-5600K)

Mode Color/White Light CCT Value

1 5600 K

Preprogrammed Modes for Mixed RGB Output Type (B190-RGB or B210-RGB)

Mode Color/White Light CCT Value

1 3000K

2 4500K

3 6500K

4 Red (635nm)

5 Green (521nm)

6 Blue (465nm)

Basic Operation

1. Follow HARDWARE SETUP to properly install the B-series illuminator onto your application system.

2. Press the ON/OFF Rocker Switch on the control panel to the “ON” position to turn the illuminator on.

NOTES: The illuminator is now on with a shutter closed. No light output will be observed now unless you turn the shutter on.

3. Toggle on the LED Shutter Button - Green light signal will illuminate on the button when the shutter is open. The illuminator will operate at the setting indicated by the control knob.

4. Use the Color Tuning (color/mode) knob to select the desired preprogrammed colors, white light CCT values. These knob settings are also referred to in this manual as preprogrammed modes. (See Preprogrammed Modes for mode descriptions. Rotating the Color Tuning knob clockwise will progress through these modes in the order listed.)

5. The Intensity knob is used to manually adjust the intensity or brightness of the modes without changing its color parameters. Turning the knob fully clockwise results in maximum brightness of the selected mode. Turning the knob fully counter-clockwise results in dimming the selected mode to zero output.



Copyright © 2016 Software Operation via USB (Virtual COM Port)

SOFTWARE OPERATION via USB (Virtual COM Port) The B-series illuminator communicates with the PC via USB (Virtual COM). The appropriate driver named “USB Serial Port” will be automatically installed when you connect the B-series via USB. If you encounter issues with the driver, please visit: http://www.ftdichip.com/Drivers/VCP.htm and download the most recent drivers. There are several ways of controlling the B-series via USB. You can easily send serial commands as illustrated below or use our B-series Windows software which you can download from our website. If using other third-party software, please contact them or us for compatibility and support. NOTES: You do not need the manual controller to do the operation. It does not matter if you connect it or not. But if you want to connect with a manual controller, please turn the rocker switch to “OFF” position.

Virtual COM Port driver Installation

Auto installation through Windows Update 1. Power on B-series illuminator and connect USB cable to PC.

OS will install driver automatically.

http://www.ftdichip.com/Drivers/VCP.htm




Manual installation 1. If OS failed to install the driver from Windows Update, please download driver from FTDI

website. http://www.ftdichip.com/Drivers/VCP.htm

2. After downloading the driver, extract the folder to the desktop.

3. Right click the unknow device “FT232R USB UART” in the device manager and select

“Update Driver Software…”

http://www.ftdichip.com/Drivers/VCP.htm




4. Select “Browse my computer for driver software”.

5. Select the folder which is extracted on the desktop and click “OK”.

6. Click “Next” to start installing the driver.




7. Driver installation is in process.

8. First driver “USB Serial Converter” has been installed.




9. You should see another unknown device “USB Serial Port” in the device manager.

Please update driver with the same driver path as mentioned above.

10. Second driver “USB Serial Port” has been installed. Now you can start to use VCP.




Serial Commands

Message Structure The message formats is the same in both the send and receive directions. Each message starts with a sentinel Start byte followed by a message length byte, and ends with a checksum byte and sentinel Stop byte. The message length value excludes the checksum byte and the start and stop bytes. The checksum value is the negative two’s complement of the sum of all the data in the message including the length byte, but excluding the Start and Stop bytes. The value of the Start byte is 0xA9, and the value of the Stop byte is 0x5C. The drawing below illustrates the message format for the B-Series illuminator.

Byte Number

Description

1 Start Byte. This sentinel value signals the beginning of a message. The value is 0xA9.

2 Message length. This value is the count of all of the bytes, excluding the Start byte, checksum, and Stop byte. The length includes the length byte itself. In the illustration above, the length value is (N-3).

3 Command byte. This value is the command code for the message.

4 to N-2

Messages to the device Messages from the device

Parameter data. This is optional data for possible command argument parameters.

In messages from the device to the host, byte B:4 will contain the command result code. Refer to Section 6 for the meaning of the result code. The remaining data, bytes B:5 to B:N-2, is optional reply data. For example, the answer data to a query command would be included here.

N-1 Checksum. The checksum is the negative sum of all the data in the message including the length byte, but excluding the Start and Stop bytes. In the illustration above, it would be the negative sum of bytes B:2 to B:N-2.

N Stop Byte. This sentinel value signals the end of a message. The value is 0x5C.




USB Virtual COM Port (FTDI FT232RQ) The USB Virtual COM Port Vendor ID for the B-Series illuminator is 0x0403. The USB Virtual COM Port Product ID for the B-Series illuminator is 0x6001.

Commands Naming Rule：

SAVE_xxxxxx：Saves data to non-volatile memory (such as EEPROM, Flash… etc.)

READ_xxxxxx：Returns data from non-volatile memory (such as EEPROM, Flash… etc.)

SET_xxxxxx：Sets data to volatile memory (such as RAM…etc.)

GET_xxxxxx：Returns data from volatile memory (such as RAM…etc.)

The following firmware command codes are currently defined:

Value Name

9 READ SERIAL NUMBER

11 READ FW TYPE

12 READ FW VERSION

31 READ AVAILABLE CHANNELS

38 GET CONTROL MODE

39 SET CONTROL MODE

65 READ HW REVISION

69 GET TEMPERATURE

73 SET CHANNEL INDEX

74 GET CHANNEL INDEX

76 READ MIN CHANNEL INDEX

77 PLAY PRESET COLOR

78 SET ALL CHANNEL INTENSITY

80 READ CHANNEL CALIBRATION

82 READ PRESET CALIBRATION

83 SET MANUAL CONTROLLER LOCKOUT

84 GET MANUAL CONTROLLER LOCKOUT STATE

85 SET CHANNEL SHUTTER

86 GET CHANNEL SHUTTER

87 SET MANUAL CONTROLLER STATE

89 SET GET MANUAL CONTROLLER SHUTTLE

91 SET EXPOSURE PARAMETER

Note: All parameter and reply values are in big-endian format. The most significant byte is first, and the least significant byte is last.




READ SERIAL NUMBER (command = 9) Command Parameters None Reply Data Serial Number String. The serial number string is variable length and it

is not null-terminated. Function Returns the device’s serial number string.

Note: This value is stored in non-volatile memory. READ FIRMWARE TYPE (command = 11) Command Parameters None Reply Data Firmware type string(9 bytes) that is not null-terminated. Function Returns the device’s firmware type string.

Note: This value is stored in non-volatile memory. READ FIRMWARE VERSION (command = 12) Command Parameters None Reply Data The device firmware version (4 bytes) Function Returns the device’s firmware version.

Note: This value is stored in non-volatile memory. READ AVAILABLE CHANNELS (command = 31) Command Parameters None Reply Data Channel count Function Returns number of channels on LED array.

Note: This value is stored in non-volatile memory.




GET CONTROL MODE STATUS (command = 38) Command Parameters None. Reply Data Control mode (1 byte)

Bit0 : indicate the shutter source 0 = controlled by TTL 1 = controlled by USB command

Bit1: indicate the intensity source 0 = controlled by Analog interface 1 = controlled by USB command

Bit7: indicate the manual control box 0 = manual control box is inactive 1 = manual control box is active

Function Returns the shutter and intensity control source. SET CONTROL MODE (command = 39) Command Parameters Control mode (1 byte)

Bit0 : decide the shutter source 0 = controlled by TTL 1 = controlled by USB command

Bit1: decide the intensity source 0 = controlled by Analog interface 1 = controlled by USB command

Function Set the shutter and intensity control source. The manual control box override all action, so manual control box is active the command is invalid. Use command 83 or turn off manual control box to inactive manual control box.

READ HARDWARE REVISION (command = 65) Command Parameters None Reply Data Hardware revision string(2 bytes),that is not null-terminated. Function Returns the device’s hardware revision.

Note: This value is stored in non-volatile memory. GET TEMPERATURE (command = 69) Command Parameters None Reply Data Temperature in 0.1 degrees Celsius (2 bytes). Function

Returns the temperature measured on the LED array.




SET CHANNEL INDEX (command = 73) Command Parameters Three 2-byte values, one for each channel. Although each parameter is

two bytes, each index is limited 1679. Function Set index for all channels GET CHANNEL INDEX (command = 74) Command Parameters None Reply Data Index for all channels, 2 bytes per channel Function Returns index for all LED channels READ MIN CHANNLE INDEX (command = 76) Command Parameters Channel number (1 byte) Reply Data Channel index (2 bytes, 0~1679) Function Returns minimum index that LEDs turn on.

Note: This value is stored in non-volatile memory. PLAY PRESET COLOR (command = 77) Command Parameters Preset color (1 byte)

Intensity level (1 byte) Function Play the preset color at the specified intensity. Note This is the preferred command for operating the B190/B210 illuminator. Note For an RGB illuminator, the preset colors are as follows:

Preset Color Number Color

1 3000K

2 4500K

3 6500K

4 Red

5 Green

6 Blue

SET ALL CHANNEL INTENSITY (command = 78) Command Parameters Channel 1 intensity (1 byte)

Channel 2 intensity (1 byte) Channel 3 intensity (1 byte)

Function Sets each channel according to the specified intensity.




READ CHANNEL CALIBRATION (command = 80) Command Parameters Channel Number (1 to 3)

Intensity level (1 byte) Reply Data Channel index (2 bytes, 0~1679) Function Returns index for the specified channel and intensity level.

Note: This value is stored in non-volatile memory. READ PRESET CALIBRATION (command = 82) Command Parameters Preset color (1 to 6)

Intensity level (1 byte) Reply Data Ch1 index (2 bytes, 0~1679)

Ch2 index (2 bytes, 0~1679) Ch3 index (2 bytes, 0~1679)

Function Returns the index for specified preset number and intensity level. Note: This value is stored in non-volatile memory.

SET MANUAL CONTROLLER LOCKOUT (command = 83) Command Parameters Manual Controller Lockout State (1 byte)

0 = Manual Controller Enabled 1 = Manual Controller Locked Out (disabled)

Function Set the manual controller lockout state. If the manual controller lockout state is enabled, the illuminator will not ignore illumination commands from the manual controller.

GET MANUAL CONTROLLER LOCKOUT (command = 84) Command Parameters None. Reply Data Manual Controller Lockout State (1 byte)

0 = Manual Controller Enabled 1 = Manual Controller Locked Out (disabled)

Function Returns the manual controller lockout state




SET CHANNEL SHUTTER (command = 85) Command Parameters Shutter state(1 byte) Function Sets individual channel shutter state. There are 8 bits in one byte, but

only the last 3 bits are used. The last bit which is bit 0 is mapped to CH1, and the bit 1 is mapped to CH2, meanwhile bit2 is mapped to CH3. The bit value is zero that mean the corresponding channel is off, otherwise is on. Only the device enter USB shutter controlled, this command is valid.

GET CHANNEL SHUTTER (command = 86) Command Parameters None. Reply Data Shutter state(1 byte) Function Returns the individual channel shutter state. There are 8 bits in one

byte. Only the last 3 bits are valid. The last bit which is bit 0 is mapped to CH1, and the bit 1 is mapped to CH2, meanwhile bit2 is mapped to CH3.The bit value is zero that mean the corresponding channel is off, otherwise is on.

SET MANUAL CONTROLLER STATE (command = 87) Command Parameters All control enable flag (1 byte)

0 = disable 1 = enable

Function Sets illuminator could be controlled by manual controller.

SET MANUAL CONTROLLER SHUTTLE (command = 89) Command Parameters All control enable flag (1 byte)

0 = disable 1 = enable

Function Turns on/off illuminator shuttle by manual controller.

SET EXPOSURE PARAMETER(command = 91) Command Parameters CH1 intensity(1 byte)

CH2 intensity(1 byte) CH3 intensity(1 byte) CH1 turn on time (4 bytes) CH2 turn on time (4 bytes) CH3 turn on time (4 bytes)

Function Set 3 channels intensity and turn on time. The turn on time unit is millisecond. During exposure period, all command is invalid. Manual control box must be turned off, this command is valid.




Error Codes The command result codes will be one of the following values:

Value Description

0 No error

1 Unknown command

2 Illegal command

3 Not enough Command Parameters

4 Too many Command Parameters

5 The message checksum is incorrect

6 The message length is incorrect

7 The message is malformed

8 A command argument is invalid

9 A command argument is out of range

100 Illegal EPROM address

101 Illegal EPROM data length

102 EPROM is busy

Environment Setup Before connecting to B-series fixture through Virtual COM Port, please check port setting is as below: Port Name: COM1 (please check device manager to get correct COM Port number) Baud Rate: 115200 Parity: None Data Bits: 8 Stop Bits: 1




Serial Command Examples Below is a series of example commands that allow you to read, get and set. Read firmware version

Start Length Command ID Checksum Stop

Dec 169 2 12 242 92

Hex A9 02 0C F2 5C

Read hardware version


Dec 169 2 65 189 92

Hex A9 02 41 BD 5C

Get Control Mode


Dec 169 2 38 216 92

Hex A9 02 26 D8 5C

Set both shutter source/intensity source to USB command (Virtual COM Port)

Start Length Command ID Parameter Checksum Stop

Dec 169 3 39 3 211 92

Hex A9 03 27 03 D3 5C

Set all CH1/2/3 shutter to open

Start Length Command ID Parameter Checksum Stop

Dec 169 3 85 7 161 92

Hex A9 03 55 07 A1 5C

Set CH1 intensity to 1

Start Length Command ID Parameter#1 Parameter#2 Parameter#3 Checksum Stop

Dec 169 5 78 1 0 0 172 92

Hex A9 05 4E 01 00 00 AC 5C




Serial Command Examples using AccessPort Software You can easily control B-series fixture through AccessPort and other software. Please download AccessPort free software from http://www.sudt.com/en/ap/download.htm 1. Power on B190/B210 and connect to PC via USB cable. (OS will install proper driver automatically.) 2. Check device manager and remember actual COM Port number.

3. Open AccessPort\Tools\Configuration and select correct information, then press OK.

http://www.sudt.com/en/ap/download.htm




4. Switch output format to Hex.

5. Now you can input command in below blank space. 5.1 Read firmware version. Input A9 02 0C F2 5C and click “Send”. Fixture returns firmware version is 0.9.3. Check below returned command format.

Start Length Command ID Error Code Parameter#1 Parameter#2 Parameter#3 Checksum Stop

Dec 169 6 12 0 0 9 3 226 92

Hex A9 06 0C 00 00 09 03 E2 5C




5.2 Read Hardware version. Input A9 02 41 BD 5C and click “Send”. Fixture returns hardware version is A.1.

(Hex)41 means [ASCII]“A” and (Hex)31 means [ASCII]”1”.

5.3 Get Control Mode. Input A9 02 26 D8 5C and click “Send”. Fixture returns 0. 0(Binary : 0000) means SHUTTER source(bit0 : 0) is TTL

INTENSITY source(bit1 : 0) is Analog.




5.4 Set both shutter source/intensity source to USB command (Virtual COM Port). Input A9 03 27 03 D3 5C and click “Send”.

5.5 Set all CH1/2/3 shutter to open. Input A9 03 55 07 A1 5C and click “Send”.




5.6 Set CH1 intensity to 1. Input A9 05 4E 01 00 00 AC 5C and click “Send”.

Now you can see B190/B210 fixture lights up channel 1 with minimal intensity.




Serial Command Examples using uManager Connect a powered B190/B210 to the computer with mini-USB cable first. If this is the first time connecting to computer, operating system will install Virtual COM Port driver automatically as mentioned in the Virtual COM port driver installation section. Wait for the COM Port driver to be installed properly. (you will see a new COM Port appear in device manager)

Before using the virtual COM Port in u-Manager, you have to configure B190/B210 as “FreeSerialPort” device. Please follow the below steps to do so. 1. On the u-Manager main page, click Tools\Hardware Configuration Wizard…




2. Select “Create new configuration” and click “Next”.

3. Find “FreeSerialPort”/select FreeSerialPort | Free-form communication Serial Port and

click “Add…”




4. Input proper setting for B190/B210 Virtual COM Port.

- Label : just input what you want, no function impact. - Value : Choose proper COM Port which is defined by operating system.

(you can find the COM Port in Device Manager)

5. Once you’ve slected COM Port, Port Properties window will show up immediately.

- You just need to select “115200” value for BaudRate and click “OK”. - Please do NOT change any other value.




6. (Now you can see B190/B210 VCP is listed in Installed Devices list.)

Click “Next” to go next step.

7. Press “Next” button to go to next step.

8. Press “Next” button to go to next step.




9. Press “Next” button to go next step.

10. Last step you have to browse a location and input file name to save this configuration.

11. Click “Finish” button to finish Hardware Configuration Wizard for B190/B210.




12. Return to main page of u-Manager, and click Tools\Script Panel…

13. Beanshell scripting window will pop up, basically it’s empty for first time using.




14. Please download .bsh sample code from http://www.scopeled.com/resources/downloads.php

Unzip the files and you should see .bsh sample code files.




15. Click “Add” button and browse .bsh files (which includes in Beanshell sample code.zip.)

Next, choose one file/click “Open”, thus you’ve added one file successfully. Please add the files one by one until all files have been put in script shortcuts on left panel.




***Note : Before going to next step to run the scripts, please manually change port number to your own virtual COM Port for ALL scripts and click “Save” to save the files. Example)

- If B190/B210 VCP is COM2, then modify the code of line 11 as below : port = “COM2” ;

- Click “Save” to save the modification. - Modify ALL scripts of COM Port and save them.




16. Once All .bsh sample has been put in Script-Shortcuts, there are two methods to use them.

- Double-click the desired script, command will be sent to B190/B210 immediately.

- Select(highlight) the script you want to run and click “Run”, command will be sent to

B190/B210 immediately.




Script running demo : 1. Read Firmware Version

Double-click “ReadFirmwareVersion.bsh” file and send out the command to VCP. You can see returned byte data is shown in the bottom of beanshell window. According to command set definition, B190/B210 responds with current firmware version which is “1.2.2” as below example.

2. Turn on the light

2.1 Set Shutter/Intensity source to USB. Default setting of shutter/intensity source is external TTL/Analog, in order to implement USB control, we have to first change both source to USB. Double-click “SetSourceToUSB.bsh” file and send out the command to VCP.




2.2 Check if control source is switched to USB. At step 2.1, we execute command to switch source to USB. In order to validate system is set up correctly, we can use Get Control Mode command. Double-click “GetControlMode.bsh” file and send out the command to VCP. System returns the information which both shutter/intensity source has been switched to USB.

2.3 Open shutter Next we open shutters for all 3 channels. Double-click “SetAllShutterOpen.bsh” file and send out the command to VCP. At this memont all shutter has been opened.




2.4 Set up intensity value Next we set up intensity index=1 for all 3 channels. Double-click “SetAllChannelIndex=1.bsh” file and send out the command to VCP. You can see every channel is lighted up at index=1.

All scripting files has been confirmed to work well with u-Manager 1.4.22 and B190/B210. To customize any other scripts, you just need to modify following values and save as a new file : 1. Correct COM Port. 2. Correct command including corresponding parameters.

(Checksum will be generated automatically by function call.) (Section : Serial Command Sending/Page.13)




ScopeLED® B-Series Software for Windows

ScopeLED

® B-series Software for Windows is a software application designed to control the

preset color through the USB interface on the Microscope Illuminator. It provides a convenient interface for controlling the B-series. Before installation, be sure that your computer is equipped with ScopeLED

® USB Communications Adapter (Available at:

http://www.scopeled.com/resources/downloads.php) and the Microsoft .NET Framework 3.5 or higher. If you need external trigger operation, please refer to EXTERNAL INPUT CONNECTOR to connect the necessary items to your device.

Installation

No installation is required. To start the software please download from the ScopeLED

® website

http://www.scopeled.com/resources/downloads.php. After download complete, unzip the folder and double click “B-Series.exe” executive file in the folder. By default, when you connect the B-Series illuminator to your computer, it will scan the COM Port number you connect to the computer. (If not, please see HARDWARE SETUP & Basic Operation for connections check.)

http://www.scopeled.com/resources/downloads.php

http://www.scopeled.com/resources/downloads.php




Features

Index: Intensity control attribute from 0~ 255. (8 bit)

DAC: Digital to Analog Converter. Intensity control attribute from 0~ 1679.

Min DAC: Minimum DAC value, which means the lowest intensity attribute to turn on the individual channel.

Shutter: The opening control of each channel. If the shutter is closed, light is off. If the shutter is open and the intensity value is more than zero (under index control) or more than min DAC (under DAC control), you will see light on.

Shutter Controls “OPEN”: All channels are off, to click “OPEN” to open the shutter .

You can choose to turn all the channels on by clicking the left control button under the “Shutter” or turn individual channels on by clicking the right three control buttons under the “Shutter.”

Shutter Controls “CLOSE”: All channels are on, to click “CLOSE” to close the shutter. You can choose to turn all the channels off by clicking the left control button under the “Shutter” or turn individual channels off by clicking the right three control buttons under the “Shutter.”

Shutter Status (example): Channel 1 and Channel 2 shutters are closed; Channel 3

shutter is opened.

CH1+2+3 CH2

CH1

CH3

CH1+2+3

CH1

CH2

CH3




Intensity: Individual channel intensity control from index 0 ~ index 255 or DAC 0 ~DAC 1679. Under Mixed RGB model type, CH1 Color = Red, CH2 color = Green, CH3 color = Blue. Under Single Color model type, CH1 Color = CH2 color = CH3 color. Before you do the channel(s) controls, make sure your shutter(s) corresponding to the channel(s) are in the “ON” status.

Intensity Controls “ON”: All channels intensity is zero, to click “ON” to turn the

channel intensity to complete on. You can choose to turn all the channels complete on by clicking the left control button under the “Intensity” or turn individual channels complete on by clicking the right three control buttons under the “Intensity.” You can also drag the bar to control the individual channel’s intensity you desire.

Intensity Controls “OFF”: All channels intensity is not zero, to click “OFF” to turn

the channel intensity to complete off. You can choose to turn all the channels complete off by clicking the left control button under the “Intensity” or turn individual channels complete off by clicking the right three control buttons under the “Intensity.”

CH1+2+3 Shutter or individual Shutter must “ON” accordingly

CH1

CH2

CH3

CH1+2+3

CH1

CH2

CH3

CH1+2+3




Intensity Status (example): Channel 1 and Channel 2 shutters are opened and with intensity index of 255 and 255, respectively. If this is a Mixed RGB model, you might see a light combination of Red (CH1) and Green (CH2), which could be pink, purple or magenta light.

Color Control: An alternative control of manual controller, which controls preprogrammed color mode and light intensity.

Shutter Source: By COM Port or TTL (See External Trigger Mode Operation)

Intensity Source: By COM Port or Analog (See External Trigger Mode Operation)




Color Control Operation: To control the preprogrammed color mode operation

1. Open ScopeLED® B-series Software for Windows program

2. Open the shutter(s).




3. Choose the color you want (Mixed RGB only). Select the shutter source. If you select TTL shutter source, please control from your signal source.

4. Select the intensity source. If USB, then control with the intensity bar. If by Analog, please control the external signal source from 0 to 6 volts.



Copyright © 2016 External Input Connector

EXTERNAL TTL/ANALOG

External Input Connector Overview The external TTL and Analog inputs have the highest priority of all modes of operation and will override any settings on the the manual controller and PC. This allows you to easily interface with hardware such as National Instruments DAQs and Arduinos. The external input connector operates though a 15-pin D-Sub connector depicted in figure below. Pin references are listed in Table1. Pin 1 to Pin 4 is for Transistor-Transistor Logic (TTL) signal input. Pin 7 to Pin 10 is for Analog control. Pin 5/6/11/12/16 is ground. Both TTL and Analog controls are with individual channel or Master (preprogrammed color mode).

Table 1: External Input Pin Configuration for Mode Control

Pin Function Input Signal Type

1 TTL1 (CH1) TTL (0-6V)

2 TTL2 (CH2) TTL (0-6V)

3 TTL3 (CH3) TTL (0-6V)

4 TTL Master TTL (0-6V)

7 Analog 1 (CH1) Analog (0-6V)



10 Analog Master Analog (0-6V)

5/6/11/12 Ground

NOTES:

Ground connection pulled to each Analog and TTL BNC cable.

The response time from the high voltage trigger to the output response of the Microscope Illuminator can be less than 2 us. (TTL shutter switch time). The Analog sampling rate is 5 Hz. (us = micro second, Hz = Hertz)

The analog control is from 0 to 6 volts, which corresponds to 0% to 100% light intensity. Please do not drive with more than 6 volts, or you may damage your unit.




Example of External Trigger Mode Operation

Uses Manual Controller to Set Channel Intensity and TTL to Shutter

1. Follow HARDWARE SETUP to properly install the B-series illuminator onto your

application system.

2. Connect the DE-15P to 2-BNC Male Cable (for single color type) or DE-15P to 8-BNC Male Cable (for RGB color type) to D-Sub connector on the LED illuminator (if TTL or Analog control is necessary.)

3. Set Manual Controller to “On” position to turn the illuminator on.

NOTES: The illuminator is now ON with a shutter closed. No light output will be observed now unless you manually open the shutter via the shutter button or trigger via TTL. When triggering using TTL, Shutter button must be in CLOSE state (not illuminated green)

4. Use the Color Tuning (color/mode) knob to select the desired preprogrammed colors, white light CCT values. These knob settings are also referred to in this manual as preprogrammed modes. (See Preprogrammed Modes for mode descriptions. Rotating the Color Tuning knob clockwise will progress through these modes in the order listed.)

5. The Intensity knob is used to manually adjust the intensity or brightness of the modes without changing its color parameters. Turning the knob fully clockwise results in maximum brightness of the selected mode. Turning the knob fully counter-clockwise results in dimming the selected mode to zero output.

6. Connect the Male BNC connectors to a BNC breakout box or TTL signal generator for the Channel or Channels desired to be triggered

7. Ensure that the Shutter Button is CLOSE (not illuminated).

8. Send TTL signal to the desired channel for TTL triggering.

Master – Triggers all three channels for white light CCT channel selection TTL1 – Triggers the Red Color Channel only TTL2 – Triggers the Green Color Channel only TTL3 – Triggers the Blue Color Channel only

9. The intensity Dial will work during the TTL triggering and intensities can be changed

during trigger generation for illumination control.

Use of TTL and Analog Control for Shutter and Intensity

1. Follow the Uses Manual Controller to Set Channel Intensity and TTL to Shutter instruction to STEP 7.

2. Connect the desired Channels TTL and Analog BNC connectors to BNC break out box or to a TTL signal generator and a power supply output.

3. Turn the ON/OFF Rocker Switch on Manual Controller to “OFF” position. (OR Disconnect the Manual Controller from the illuminator by unplugging the RJ12-6 connection cable.)

4. Set the desired Analog Channel from 0-6 Volts for minimum to maximum intensity levels

5. Trigger the desired channels with TTL signal generator.




USB Software Operation by ScopeLED® B-series Software for Windows

1. Follow Illuminator Mounting Installation to STEP 4 to properly install the B-series

illuminator onto your application system.

2. Connect the power adapter to the illuminator.

3. Plug the AC power cord into the power adapter and then into the wall socket.

4. Connect the DE-15P to 2-BNC Male Cable (for single color type) or DE-15P to 8-BNC Male Cable (for RGB color type) to D-Sub connector on the LED illuminator (if TTL or Analog control is necessary.)

5. Connect the desired Channels TTL and Analog BNC connectors to BNC break out box or to a TTL signal generator and a power supply output (if TTL or Analog control is necessary.)

6. Connect USB to Mini-USB Cable Cord to the operation computer and the illuminator.

7. Operating system on your PC will automatically install Virtual COM Port driver.

8. Once the driver is installed properly, please follow ScopeLED® B-Series Software for

Windows Overview to control the unit.

NOTES: You do not need the manual controller to do the operation. It does not matter if you connect it or not.

RGB image Acquisition and White Balancing (Mixed RGB model only)

1. Follow Basic Operation to setup the connections.

2. Connect USB to Mini-USB Cable Cord to the operation computer and the illuminator. (If USB control is necessary)

3. For simple RGB image acquisition and white balancing of images use one of the calibrated CCT color temperature channels 3000K, 43000K or 5600K in conjunction with image capture software and camera control.

4. Using the manual controller or by USB commands (See Programming Operation) set the channel selection to desired calibrated CCT color temperature

5. Set the illumination intensity, with the manual controller or by USB command

6. Open the channel 1 (Red) shutter by USB shutter commands or by ScopeLED® B-series

Software for Windows or by connecting TTL 1 and synchronize with a camera acquisition.

7. Open the channel 2 (Green) shutter by USB shutter commands or by ScopeLED® B-

series Software for Windows or by connecting TTL 2 and synchronize with a camera acquisition.

8. Open the channel 3 (Blue) shutter by USB shutter commands or by ScopeLED® B-series

Software for Windows or by connecting TTL 3 and synchronize with a camera acquisition.

9. Merge the three images, if necessary do a simple post acquisition white balance routine in the imaging software on a white portion of the merged image.



Copyright © 2016 Troubleshooting

TROUBLE SHOOTING

Problem Solution

Illuminator does not turn on

Check that the green LED Power Indicator on the illuminator is on and verify that all of the connections to the adapter and the Microscope Illuminator are properly connected.

Turn the Intensity knob to maximum intensity and cycle through the different modes using the color-tuning knob. The color-tuning knob can be in a custom mode position where the custom mode has not been programmed. For your mode of operation, ensure that a higher priority input is not interfering. If you’re using the manual controller only, check that the “shutter” button is OPEN (illuminated green)

Illuminator is not emitting all of the colors (Not applicable to 5600K model)

Turn the intensity knob to maximum intensity, then cycle through each preset mode using the color-tuning knob. If any of the colors appear unusually dim, please contact the manufacturer.

Illuminator is flickering

Flickering is often a result of the driver not receiving a high enough DC voltage or current. Be sure to use the power adapter from ScopeLED to avoid power shortage issue.

Illuminator has shut down automatically and is hot to touch

Make sure there is nothing obstructing the airflow to the Microscope Illuminator. It is required that a minimum of eight cubic feet of airspace is provided around the device and the unit will auto shutdown if it gets too hot.



Copyright © 2016 Specifications

SPECIFICATIONS

Absolute Maximum Ratings

Input Voltage 12VDC

Input Current 2.5A

Operating Temp 0ºC to 40ºC (32ºF to 104ºC)

Storage Temp -40ºC to 70ºC (-40ºF to 158ºF)

Humidity 10% to 90%

B190 Model Weight 0.50KG (1.1lbs)

B210 Model Weight 0.835KG (1.84lbs)

Optical Specifications

Typical Optical Characteristics – Single Color Output Type (B190-5600K)

Typical CCT / Color Settings

Light Engine

Radiant Flux (mW)

Luminous Flux (Lm)

5600K 3025 950

Typical Optical Characteristics – Mixed RGB Output Type (B190-RGB)

Typical CCT / Color Settings

Light Engine

Radiant Flux (mW)

Luminous Flux (Lm)

3000K 2695 718

4300K 2688 687

5600K 2678 666

Red (635nm) 2312 435

Green (521nm) 994 439

Blue (465nm) 1466 114

* B210 illuminator includes a diffusing rod and collector lens with adjustable focus.

Mechanical Dimensions Mechanical Dimensions for all Microscope Illuminator models

Model Dimensions (Illuminator only, unit in mm)

B190 82 (L) x 85 (W) x 110 (H)

B210 154 (L) x 85 (W) x 110 (H)

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