Yogyakarta, 26 October 2016

No : COM-ICBFSH/PSPG-UGM/X/051/2016 Subject: Letter of Acceptance

Ms. Mutiara Nugraheni

Universitas Negeri Yogyakarta

Dear Ms. Mutiara Nugraheni

It is pleasure to inform you that your paper entitled.

Antioxidant Activity of The Ethanolic Extracs of Peel and Flesh of Coleus tuberosus

Paper ID: OHHN-06, written by Mutiara Nugraheni, Badraningsih Lastariwati, and

Windarwati h as been accepted to be presented in oral presentation in the 1st International

Conference on Biodiversity, Food Security and Health (ICBFSH 2016). The conference will

be held in Yogyakarta, Indonesia on November 22-23, 2016. The committee would like to

express our sincere welcome to you.

Further information will be sent by email, also please check our website

(rce.ugm.ac.id/seminar) for update. If you have any question, please feel free to contact us.

Thank you for your participation in this conference. We look forward to seeing you in the

ICBFSH 2016.

Sincerely yours,

Dr. Lily Arsanti Lestari, STP., MP

Chairperson of ICBFSH 2016

email : icbfsh@ugm.ac.id

1st International Conference on Biodiversity, Food Security, and Health

Gadjah Mada University, 22-23 November 2016 www.rce.ugm.ac.id email: icbfsh@ugm.ac.id

Technical Session Presentation Schedule

November 23rd

, 2016 at UC Hotel UGM

Time Venue

Room 1:

Bulaksumur Room 2: Grafika Room 3: Nusantara Room 4: Yustisia

Technical Session I

Time Moderator : Dr. M.

Nur Cahyanto Moderator : Prof. Suwarno H

Moderator : Dr. Sigit

Setyabudi Moderator : Prof. Y. Marsono

10.30-10.45 OFT-01 OAA-04 OFS-01 OHN-01

10.45-11.00 OFT-02 OAA-05 OFS-02 OHN-02

11.00-11.15 OFT-03 OFT-37 OFS-04 OHN-03

11.15-11.30 OFT-04 OAA-07 OFS-05 OHN-04

11.30-11.45 OFT-07 OAA-08 OFS-06 OHN-05

11.45-12.00 OFT-32 OAA-09 OFS-07 OHN-06

Lunch Time

----------------------------------------------------------------

Technical Session II

Time Moderator : Dr. Bayu

Kanetro

Moderator : Dr. Retno Peni

Sancayaningsih

Moderator : Prof. Endang S.

Rahayu Moderator : Dr. Elisa Julianti

13.00-13.15 OFT-05 OAA-11 OFS-08 OHN-07

13.15-13.30 OFT-08 OAA-12 OFS-09 OHN-08

13.30-13.45 OFT-09 OAA-14 OFS-10 OHN-09

13.45-14.00 OFT-10 OAA-15 OFS-11 OHN-10

14.00-14.15 OFT-11 OAA-16 OFS-12 OHN-11

14.15-14.30 OFT-12 OFT-17 OFS-35 OHN-12

14.30-14.45 OFT-34 OAA-18 OFT-36 OHN-13

Coffee or tea available

Technical Session III

Time Moderator : Dr.

Supriyadi

Moderator : Dr. Chusnul

Hidayat

Moderator : Dr. Mutiara

Nugraheni Moderator : Dr. Karseno

14.45-15.00 OFT-13 OFT-27 OAA-19 OHN-14

15.00-15.15 OFT-14 OFT-28 OAA-21 OFT-23

15.15-15.30 OFT-15 OFT-29 OFT-19 OFT-24

15.30-15.45 OFT-16 OFT-30 OFT-20 OFT-25

15.45-16.00 OFT-17 OFT-31 OFT-21 OFT-26

16.00-16.15 OFT-18 OFT-06 OFT-22 OFT-33

Abstract List

Code Title Author (s)

Agrobiodiversity and Agroforestry (OAA)

OAA-01 River Corridor Cultural Ecosystem Services and Urban

Well-being in George Town, Pulau Pinang and Kangar,

Perlis

Asyirah, A.R. & Mohammad

Izzamil, M.N.

OAA-02 Food Insecurity and Ramifications of Desertification in

the Sahel: The Case with Nigeria

Usman Muhammad

OAA-03 Screening Wood-rot Fungi for Antimicrobial Property Tan T. Thai, Ngoc. V.K. Pham,

Hoa T. Pham

OAA-04 Lygodium Circinatum: Distribution Pattern and

Environment Factors Influencing its Growth in Nature

Eny Faridah, Endah

Wahyuningsi, Budiadi

OAA-05 The Role of Local Community in Conserving the Local

Biodiversity in Karst Ecosystem Case Study in Kepek

Village-Indonesia

Agus Suyanto , Nasirudin,

Chafid Fandeli, Dewi Rahyuni

OAA-06 Exploring Diversity and Potency of Endophytic

Actinobacteria from Medicinal Plants

Y. Lestari M. Rahminiwati,R.

Heryanto, M. Ernawati1, and

W.P. Sari

OAA-07 The Ethnobotany and Taxonomy Study of the Jamur

Selo (Nostoc commune Vaucher ex Bornet & Flahault)

Potentially as the Source of Single Cell Protein from

Gunungkidul, Daerah Istimewa Yogyakarta

Ludmilla F. Untari and Gustina

Dewi

OAA-08 Effects of the Botanical Insecticide Annona squamosa

L. and Curcuma domestica Val. on Spodoptera litura

F.

Siti Sumarmi and Ramadhan

Taufika

OAA-09 Application of Natural Dye From Secang Wood

(Caesalpinia sappan L) on Crust Cattle Leather by

Dyeing Method Using Emboss Techniques

Entin Darmawati, Umar

Santoso and Sudarmadji

OAA-10 Conflicting or Combinative – Human and Natural

Values at Kathotiya, Central India

Saurabh Popli

OAA-11 Analysis Physic ad Chemical Traditional Food Ilabulo

Catfish (Pangasius sp.) Fortification Seaweed

(Kappaphycus alvarezii) and Bone Meal Catfish

Rita Marsuci Harmain, Faiza

Dali, Nurjanah, Agoes

Mardiono Jacob

OAA-12 Fusarium oxysporum f.sp.cubense Tropical Race 4 and

Race 1 Resistance in Indonesian Musa acuminata

Fajarudin Ahmad, Yuyu S

Poerba, Hans de Jong, Hugo A

var.malaccensis and Self-pollinated Progenies

Volkaert, Gert HJ Kema

OAA-13 Negritos de Cebu : Gender, Livelihood, Resource

Management and Social Change

N. Sabuero, R. Villavelez, and

H Zanoria

OAA-14 Urban Agroforestry for Riverside in Yogyakarta : a

Potency of Smart Environment Transformation

Emmy Yuniarti Rusadi,

Mazidatun Maftukhah

OAA-15 Traditional Red Rice Grain Characteristics Still

Cultivated In Regencies Of South Sulawesi

Muhammad Riadi, Rinaldi

Sjahril, Rafiuddin, Tadashi

Sato, Kinya Toriyama,

Trisnawaty, A.R., Dwi Septiani,

Rafiuddin, Tadashi Sato, Kinya

Toriyama

OAA-16 Biodiversity Assesment of Mangrove in Pasuruan

District,

East Java

Muliawati Handayani,

Sukandar

OAA-17 Effect of lemongrass leaves (Cymbopogon citratus) as

an essential oil source on ruminal enzymes activities

Kurniawati, A., Yusiati, L. M.,

Supadmo, Setiawan, A. R.,

Zulfa, I. H.

OAA-18 Jack fruit (Artocarpus heterophyllus) leaves as

inhibitor agents of methane production inruminant by

in vitro

Anas, M. A., C. Hanim, A.

Kurniawati, Z. Barcruddin

Muhlisin

OAA-19 The Study of Black Soy Bean (Glycine max (l.) Merr)

Seed of Mallika Production by Intercropping System

with Sweet Corn

Setyastuti Purwanti and Ari

Wibowo

OAA-20 Agressiveness of Tabernaemontana macrocarpa upon

Sustainability of Two Protected Communities ofAcacia

and Pineat Mangunan Conservation Forest, Bantul,

Yogyakarta, Indonesia

Retno Peni Sancayaningsih,

Anindyasari Kumalasari, Abi

Giusti Wohing Atie

OAA-21 The Origin of Soybean in Indonesia

Mary Astuti

Food Security and Safety (OFS)

OFS-01 Growth Performance and Body Composition of Local

Beef

Firmansyah, D., A. M. Fuah, R.

Priyanto, and I K.G. Wiryawan

OFS-02 Chemical and Physical Quality of the Meat Magelang

Ducks with Feed Addition Shrimp Waste Fermentation

Amrih Prasetyo, Lies Mira

Yusiati, Yuny Erwanto,

Wihandoyo, and Rusman

OFS-03 Community Mobilization and Utilization of

Information Technology in Scaling-up Resource

Management and Disaster Preparedness

Huberto C. Zanoria ( MCC ), A.

Diola ( UC ), M. Pielago (

CSWDO-Mandaue City ) and

R. Villavelez ( SWU )

OFS-04 Chemical, Biological Activity and Heavy Metal

Content of Sea Cucumbers from Karimunjawa and

Lampung’s Marine, Indonesia

Ekowati Chasanah,

Kustiariyah, Hedi Indra Januar,

Yusro Nuri Fawzya, Rini

Susilowati, Muhammad Nursid

OFS-05 Influence of Diameter Die, Binding Matterial, Hot

Water Addition and Sliced Oil Palm Frond on the

Quality of Feed Ruminants Pellet from Oil Palm

Biomass

Giyanto

OFS-06 Nutritional Value and Albumin Content of Merauke’s

Swamp Fish

Rini Sulistiyowati,Sugiyono

and Ekowati Chasanah

OFS-07 Pathogenic Bacteria Contamination of Loin Bali Cattle

That Slaughter at Modern and Traditional System

Sriyani NLP, Artiningsih R. M,

A.A Oka, Tirta Ariana, Sayang

Yupardi, Linda Anggreni

OFS-08 Food Safety of Bali Beef Reared on Waste Disposal

Area

Tirta Ariana IN., NLP.Sriyani,

Gd.Suarta.,AA.Oka,

S.A.Lindawati, G.A.M.Kristina

Dewi

OFS-09 Risk Assessmenton Handling System Small and

Medium Enterprises (SMEs): Case Study onTraditional

Coconut Sugar Industry in Yogyakarta

Muhammad Prasetya

Kurniawan, Wahyu Supartono,

Anggoro Cahyo Sukartiko,

Wildan Fajar Bachtiar, and

Sanyoto

OFS-10 Promoting Sustainable Agriculture in Pekalongan,

Indonesia :

Coastal Farmers Choices

Artiningsih, Suratman, R

Rijanta, Su Rito Hardoyo

OFS-11 Development and Validation of a Rapid HPLC-FD

Method for the Simultaneous Determination of

Serotonin and Its Precursors

Duanti Oktarani Tisadewi,

Pargiyanti, Muhammad Khak,

Widiastuti Setyaningsih

OFS-12 HALAL INDUSTRIAL PARK (HIP): Strategic

Movement for Food Security by Building Competency

of Khalifah Industry

Ivan Lanovara & P.L. Rika

Fatimah

Food Technology (OFT)

OFT-01 Effect of Peeling, Sodium Metabisulphite Pretreatment

and Drying Temperature on Physical And Functional

Properties of Sweet Potato Flour

Elisa Julianti, Herla Rusmarilin,

Ridwansyah, Era Yusraini,

Syahdian Lestari

OFT-02 Effect of Weather Conditions and Variations of Methyl

P-Hydroxybenzoate Concentration to Chemical

Properties and Antioxidant Activity of Coconut Sap

Pepita Haryanti, Buana Handa

Wijaya, Umar Santoso,

Supriyadi and Djagal Wiseso

Marseno

OFT-03 Determination of Antioxidant Activity and Phenolic

Compounds on Methanolic Extract of Java Plum

(Syzygiumcumini l. (skeel) Seed

Rohadi, Santoso, U. Raharjo, S.

and Falah, I.I.

OFT-04 Effect of Type and Concentration of Herbal Extract

Coating on Content of Resistant Starch and Glycemic

Index of Chromium Fortified-Parboiled Rice

Wisnu Adi Yulianto, Ch. Lilis

Suryani, Mamilisti Susiati, Sri

Luwihana

OFT-05 The Effect of Green Betel Leaf Extract, Mangosteen

Rind Extract, and Lime Solution Addition as Natural

Preservative on Coconut Sugar Quality

Karseno and Retno Setyawati

OFT-06 Enhancing Antioxidant Activity of Indonesian Sesame

Oil (Sesamum indicum) by Heat Treatment

Aldila Din Pangawikan, Umar

Santoso, Suparmo, Pudji

Hastuti

OFT-07 Development of Home Industry of Growol-Oyek into

Artificial Rice in the Village of Kalirejo Kulon Progo

Bayu Kanetro, Dwiyati

Pujimulyani, Alimatus Sahrah,

Sri Luwihana

OFT-08 Exterior and Interior Egg Quality of Muscovy Duck

(Cairina moschata) Reared Traditionally in

Yogyakarta

Nurliyani, Widodo, Satyaguna

Rahmatulloh, Indratiningsih

OFT-09 Chemical Characteristics during the Ripening Process

Coconut Sap Became Coconut Sugar Crystals with

Addition Variation Preservatives Acid Methyl P-

Hydroxybenzoates

Beta Alfisyahri Putri, Pepita

Haryanti, Umar Santoso dan

Supriyadi

OFT-10 Evaluation on Antioxidant Activity of Beef Liver

Protein Hydrolysates

Khothibul Umam Al Awwaly,

Yuny Erwanto, Wayan T.

Artama, Rusman

OFT-11 The Effect of Porang (Amorphophallusoncophyllus)

Glucomannan and Carboxymethyl Cellulose (CMC)

Addition on Physical and Sensory Characteristics of

Wet Noodlewith Mocaf (Modified Cassava Flour)

Substitution

Silvana Yasinta Putri, Zaki

Utama, Sri Rahayoe, Eni

Harmayani

OFT-12 The Effect of Edible Coating Materials and Pandan

Extract Concentrations on Cooking Quality of

Parboiled Rice Fortified with Chromium, Magnesium

and Vitamin D

Wisnu Adi Yulianto, Sri

Luwihana, Mamilisti Susiati,

Arka Jati Laksana

OFT-13 Utilization of Potato Flour from Variety Granola for

Potato Chips Production

Condro Wibowo, R. Wicaksono

and Erminawati

OFT-14 Variation in Linier Body Measurements of Pasundan

Cattle in West Java

N. Erni, R. Priyanto and Jakaria

OFT-15 Stability of Serotonin and Its Precursors in Ethanolic

Solution and Extract from Black Rice (Oryza Sativa L.)

Nalaputi Basoeki, Pargiyanti,

Muhammad Khak, Widiastuti

Setyaningsih

OFT-16 Characterization of Gathotan Enzyme for Starch

Modification

Umi Purwandari, Darimiyya

Hidayati, Kufah Nur Afifah,

Jayanti, Endry N. Prasetyo

OFT-17 Effect of Gamma Irradiation on Antioxidant Levels of

Civet Coffee Using 2,5kgy; 5 Kgy; 10 Kgydoses

Jepri Sutanto, Musaddiq

Musbach, Sugiyarto

OFT-18 Extraction of Dry Lutein from Red Spinach’s Roots,

Stems and Leaves As Functional Food Ingredients

Andi Nur Faidah Rahman,

Rindam Latief, Andi Dirpan.

OFT-19 Processing of Food Grade Semirefined Carrageenan

and Its Quality

Murdinah and Subaryono

OFT-20 Effect of Pretreatment by Microwave on Polyphenol

Content and Antioxidant Activity on Fresh Cocoa

Beans.

Nurhayati, FMC Sigit

Setyabudi, Djagal Wiseso

Marseno, Supriyanto

OFT-21 Identification of Sheepmeat Flavour and Odour with

Divergent Fat Content in Javanese Fat Tailed

Asep Gunawan, Jakaria, Kasita

Listyarini, Cece Sumantri, and

Muhammad Jasim Uddin

OFT-22 Copigmentation of Anthocyanin Extract of Java Prune

(Kopsia pruniformis) Fruit with Quercetin to Increase

the Colour Stability

Lydia Ninan Lestario, Yosevin

Panggola, Silvia Andini

OFT-23 Seed Size Reduction Effect on Chemical Characteristic

of Sword Bean (Canavalia gladiata) Tempeh : Total

Phenol, Antioxidant Activity, Dietary Fiber

Dwi Ishartani, Dian

Rachmawanti, Edhi

Nurhartadhi, Asri Nursiwi,

Ardhea Mustika Sari

OFT-24 Characterization of Fucoidan Extracted from

Lampung’s Brown Seaweeds

Ellya Sinurat, and Rinta K

OFT-25 Preliminary Investigation of the Use of Dried Kefir

Culture to Manufacture Fermented Sausages

Juni Sumarmono, Mardiati

Sulistyowati, Kusuma

Widayaka, AHD Djoko

Rahardjo, and Triana

Setyawardani

OFT-26 Effect of Autoclaving-cooling Cycle on Resistant

Starch Content and Functional Properties of Gayam

(Inocarfus fagifer Forst.) Flour

Agus Wijanarka, Toto Sudargo,

Eni Harmayani and Yustinus

Marsono

OFT-27 Characteristics and viability of dry starter prepared

from isolated indeginousmicroorganism to improve

cocoa fermentation process

Yusya’ Abubakar, Heru P.

Widayat, Martunis, M. Muzaifa,

R. Anggraini

OFT-28 Extract corn silk with variation of solvents on yield,

total phenol, antioxidant total flavonoids and

antioxidant activity

Haslina and Murtiari Eva

OFT-29 Evaluation of Anthocyanin Stability, Psycochemical

Properties and Organoleptic Characteristics in Ketan

Tape with Additional Erpa (Aerva sanguinolenta)

Leaves Extract

Norma Dwi Septian, Umi Noor

Rohmah, Andriati Ningrum

OFT-30 Protein Binding Capacity of Three Forages as Tannin

Sources and Its Effect on in vitro Nutrients

Digestibility

Yusiati, L. M., Kurniawati, A.,

Bachruddin, Z., Supadmo

OFT-31 In vitro Gas Production of Three Forages as Tannin

Sources and Its Effect on the Kinetics of Gas

Production

Hanim, C., A. Kurniawati,

Muhlisin, L. M. Yusiati, I. H.

Zulfa

OFT-32 Physicochemical and Functional Properties of Tubers

Starches

Fauzan Azima, Novizar Nazir,

Hendra Cahya Efendi

OFT-33 Physical Properties and Sensory Effect of

Glucomannan Porang (Amorphophallus oncophyllus)

and Kappa-Carrageenan Addition 0n Ice Cream

Anindhita Dewanti Nareswari,

Zaki Utama, Eni Harmayani

OFT-34 Potential Liquid Smoke of Palm’s Kernel Shell as

Biopreservative to Tuna (Thunnus sp) Fish

Musrowati lasindrang

OFT-35 Chemical Properties of Fermented Local White Corn

Flour of Anoman FS Variety

Rahmawati, Rijanti Rahaju

Maulani, Dede Saputra

OFT-36 Soybean and Pumpkin Seed on Pumpkin Seed Tempe

Shanti Pujilestari, Rimma Maria

dan Diny A. Sandrasari

OFT-37 Biofilm-forming Ability and Resistance to

Disinfectants of Samples Collected from Seafood

Processing Plants

Punnanee Sumpavapol, and

Dusida Tirawat

Human Health and Nutrition (OHN)

OHN-01 Protective Effect of Tropical Fruit Juice on

Histopathological Image of Rats Lung Exposed to

Cigarette Smoke

Novi Febrianti, Muhammad

Ilham

OHN-02 Anticancer Activity of Water Extracts of Tempe

Obtained from Different Fermentation Periods

Zatil Afrah Athaillah, Anastasia

F. Devi, Dian Muzdalifah,

Wirasuwasti Nugrahani,

Linar Zalinar Udin

OHN-03 Hypoglycemic Effect of Sago Starch (Metroxylon spp.)

and Red Bean (Phaseolus vulgaris)-Based Analogueue

Rice on Diabetic Rats

Sri Budi Wahjuningsih, Y.

Marsono, Danar Praseptiangga

OHN-04 Food Security Intake Among Neglected Mothers:

Exploring Parental Failure in Food Feeding Practice in

Mataram

Chairun Nasirin

OHN-05 Water Quality and the Risk of Waterborne Diseases in

Teros Village, East Lombok Regency, West Nusa

Tenggara Province

Baiq Liana Widiyanti,

Ig.L.Setyawan Purnama, Adi

Heru Sutomo, Setiadi

OHN-06 Antioxidant Activity of the Ethanolic Extracs of Peel

and Flesh of Coleus tuberosus

Mutiara Nugraheni,

Badraningsih Lastariwati,

Windarwati

OHN-07 The Effect of Solvent Extraction on the Antioxidant

Properties of Melon (Cucumis melo L.) cultivar

Hikapel

Puji Wulandari, Budi Setiadi

Daryono, Supriyadi

OHN-08 A Study of Fatty Acids Profile in Crossbred Chickens

between Kampong and Broiler Chicken

Asep Gunawan, Ahmad Furqon,

Kasita Listyarini, Jakaria

OHN-09 Functional Properties of Pigmen Extract of Tamarilo

Seed Jelly on Hyperglycemic Sprague Dawley Rats

and Application on Traditional Syrup Drink

Gusti Ayu Kadek Diah

Puspawati, Gusti Ayu Ekawati,

Putu Timur Ina,

GP. Ganda Putra

OHN-10 Hypoglycemic Effect Of Dietary Fiber and Resistant

Starch from Mocaf, Arrowroot and Kidney Bean Flour

Based Analogue Rice on Diabetic Rats

Sri Budi Wahjuningsih, Haslina

and Sri Untari

OHN-11 Characteristics of Nutrition Content of Nike Fish

(Awaous melanocephalus) and Manggabai Fish

(Glossogobius giuris) as Local Raw Material for Food

Flavoring Product

Faiza A. Dali, Rita M. Harmain

OHN-12 Hair Extract Maize (Corn Silk) with Variation of

Solvents on Rendemen, Total Phenol, Antioxidant

Activity and Total Flavonoids

Haslina and Murtiari Eva

OHN-13 The Effectiveness of Various Salacca Vinegar as

Therapeutic Agent forManagement of Hyperglycemia

and Dislipidemia on Diabetic Rats

Elok Zubaidah, Tiara

Puspitasari, Umi Kalsum,

Dianawati Dianawati

OHN-14 Inhibition Activity of Mangrove Fruit (Rhizophora

mucronata)

Based on pH Against α-Glukosidase

Yunita Eka Puspitasari, Mutiara

Warda Syamsyah, Hardoko,

Bambang Budi Sasmito

Traditional Food and Knowledge (OTF)

OTF-01 The Jackfruit Festival - Reviving Jackfruit based Food

Cultures to Fight Climate Change and Assuring Food

Security

Sachin Sathyarajan

Poster Session

Code Tittle Author (s)

Agrobiodiversity and Agroforestry (PAA)

PAA-01 Rehabilitation & Conservation Environmental Based

Community : "Darling to Sustainable Forest Area and Disaster

Awareness " In the Garut District

Okke Rosmaladewi, Rubi

Robana, Lilis Irmawatie

PAA-02 Ulvan from Green Seaweed and its Biological Activity : a

review

Subaryono

PAA-03 Optimizing Potencies of Village for Agrobiodiversity

Conservation

Whisnu Febry Afrianto,

Ardhiyani Kusumastuti

Food Security and Safety (PFS)

PFS-01 Development of Thermophilic Spore-forming Bacteria

Detection Method for Thermal Processed Food

Punnanee Sumpavapol,

and Jaruporn Rakmai

Food Technology (PFT)

PFT-01 The Organoleptic Characteristic Properties Formula MP-ASI

Instant Baby Porridge with Snakehead Fish Meal and Pumpkin

Flour Substitution

Dewi Kartika Sari, Ali

Rosidi, Hafni Rahmawati

PFT-02 Effect of Banana Variation (Musa paradisiaca forme typica L.,

Musa corniculata rumph., Musa normalis L., and Musa

paradisiaca L. var. typica) on the Organoleptic and Proximate

Content of “Manggulu” (Popular Traditional Food From

Sumba)

Helmiati Ndelo, Waluyo,

Puspita Mardika Sari

PFT-03 Antioxidant Activity of Melinjo Red Peel Extract

Microcapsule That Is Applicated to Sagon

Bambang Kunarto dan

Ery Pratiwi

PFT-04 The Quality of Ronto with Used Different Salt and Rice

Concentration

Rita Khairina, Iryanti

Fatyasari

Nata, Nooryantini,S. dan

Susana Ristiarini

PFT-05 Processing of Shrimp Shell as Sources of Glukosamin by

Fermentation Method

Hardoko, Bambang Budi

Sasmito, Yunita Eka

Puspitasari, Nugroho

PFT-06 The Effect of Curing Solution on the Gel Strength of Gelatin

from Different Animal Skin

Lily Arsanti Lestari, Yuny

Erwanto, Abdul Rohman,

Yudi Pranoto

PFT-07 The Physical Properties of Gelatin from Buffalo Hide by

Pretreatment using Citric Acid

S. Mulyani, F.M.C. S.

Setyabudi, Y.Pranoto and

U. Santoso

PFT-08 Quality of Gelatin from Buffalo Skin by Pre-Treatment with

Alkali-Acid and Its Application in Jelly Candy

J. U. Ghaisani, Y. T.

Afrianti, S. Mulyani, Y.

Pranoto, U. Santosa

Human Health and Nutrition (PHN)

PHN-01 Development of Black Rice-based Beverage for the

management of type 2 diabetes mellitus in elderly

Rianita Pramitasari, Mary

Astuti, Y. Marsono, Fx.

Suharnadi

PHN-02 Isolation of Melanin from Cuttlefish and Squid Ink, and

Analysis Their Activity Against E.coli

Yuspihana Fitrial, and Iin

Khusnul Khotimah

PHN-03 Knowledge and Attitudes about Reproductive Health

(Preliminary Study of a Newlyweds in Brebes District, Central

Java, Indonesia)

Nugraheni, S.A, Martini,

Sulistyowati, E., Johan, I

PHN-04 Advanced Glycation End Products (AGEs) and the

Pathogenesis Chronic Diseases

Dina Aulia Insani, Linda

Artanti

N. Baboby et al./1st International Conference Biodiversity Proceedings, 22-23 November 2016

1

Antioxidant Activity of The Ethanolic Extracs of Peel and

Flesh of Coleus tuberosus

Mutiara Nugrahen *1 Badraningsih Lastariwati*2 and Windarwati 2 1 Departmen of Culinary Education, Yogyakarta State University, Indonesia

Email: mutiara_nugraheni@uny.ac.id 2 Departmen of Culinary Education, Yogyakarta State University, Indonesia

Email: Badraningsih Lastariwati@uny.ac.id

Abstract This study was to know total phenolic and flavonoid content and the ability of the

scavenging of free radicals in chemical tubes and in biological systems. Determination of

total phenolic and flavonoid contents were using spectrophotometric method. The method

used by the chemical of tube is 1.1-diphenyl-2picrylhydrazyl (DPPH) and biological

systems by the method of cellular antioxidant activity based on the oxidation of 2 ', 7'-

dichlorofluorescein-diacetate (DCFHDA) by reactive oxygen species (ROS) in Hela cancer cells. The results showed that total phenolic content on peel and flesh were 6.10 ± 0.02,

and 1.77 ± 0.01 mg of GA/g of extract. respectively. Flavonoids content on peel and flesh

were 3.08 ± 0.05, and 0.26 ±0.01 (mg of quercetin/g of extract, respectively. The peel

ethanolic extract of Coleus tuberosus (PEC) has higher antioxidant activity than the flesh

ethanolic extract of Coleus tuberosus (FEC) PEC that evaluated by DPPH and cellular

antioxidant method. The IC50 of PEC was 310.97±0.32 and FEC was 1290.00 ± 1.58. The

percentage decrease of ROS on 100, 200, 400 and 800 µg/ml of PEC were 44.32±0.35;

52.52±0.24; 72.77±0.31, and 80.15±0.82 respectivelly. The percentage decrease of ROS

on 100, 200, 400 and 800 µg/ml of FEC were 25.67±0.18; 42.98±0.22; 59.12±0.28, and

66.27±0.37, respectivelly. This results shows that the extract of Coleus tuberosus has

potential as a source of natural antioxidants.

Keywords: antioxidant activity, Coleus tuberosus, flesh, peel

N. Baboby et al./1st International Conference Biodiversity Proceedings, 22-23 November 2016

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1. INTRODUCTION

The human body every day is always interacting with reactive oxygen species (ROS), which is derived

from UV rays, cigarette smoke, air pollution,

radiation, drugs, metabolism of the human body, and

inflammation that can react and cause damage and mutations in the cells, oxidizes carbohydrates, lipids,

proteins and DNA (Borek et al., 2004). Reactive

oxygen species ranked highest as the main cause of the disease. Included in the ROS are superoxide anion

radicals (O2 • -), singlet oxygen (1O2), hydrogen

peroxide (H2O2) and hydroxyl radical (•OH).

Although the human body has a antioxidants defense

system namely antioxidant enzymes (superoxide

dismutase, Catalase, Glutathione Peroxidase),

vitamin E, beta-carotene, vitamin C, which has a

molecular structure that can donate electrons to molecules ROS without disturbing the stability of the

molecule and can break the chain reaction from free

radicals, but the body can still suffer from oxidative stress. Oxidative stress occurs in cells or tissue

resulting from an imbalance between the production

or concentration of ROS and antioxidant ability

in cells that can lead to oxidative damage (Manda et al., 2009). Reactive oxygen species can be captured

by the body's antioxidant defense system and

phytochemical compounds, such as phenolic, triterpenic acids, flavonoids, carotenoids, vitamin

found in fruits and vegetables.

Several studies have shown that phytochemical compounds from plants can act as an antioxidant

that can prevent the accumulation of ROS and have

a positive impact on the prevention of disease.

Research shows that antioxidants may have a positive impact on the prevention of disease also

showed an association shortage of consumption of

fruits and vegetables against the increased risk of cancer. The consumption of fruits and vegetables

provide a protective effect against the occurrence of

degenerative diseases. Efforts to protect the body against free radicals is by increasing plasma

antioxidant capacity. This can be done by

consuming vegetables and fruits that contain

phytochemical compounds that have the ability as

an antioxidant (Garinstein et al., 2009).

The growing demand for natural antioxidants in the

food and cosmetics industries, encourage efforts to find the source of natural antioxidants. Numerous

scientific investigations point at consecutive rich

sources of antioxidants, both fruits and vegetables,

but only few of them involve waste parts of fruits or vegetables, i.e. peels. Fruits and vegetables wastes

and by-products, which are formed in great amounts

during industrial processing, represent a serious

problem, as they exert an influence on environment and need to be managed and/or utilized. On the other

hand, they are very rich in bioactive components,

which are considered to have a beneficial effect on

health. So that the necessary efforts to provide information regarding the antioxidative potential of

the peel of the fruit or vegetable in the hope that the

skins of fruits and vegetables is not only a waste, but it can be a source of bioactive compounds that can be

used as a source of natural antioxidants.

Coleus tuberosus is a minor tubers are included in family of Lamiaceae, sub-family of Ocimeae and

Tribe of Nepetoide. Coleus tuberosus classified in the

group that shaped tuber vegetables. Based on

ethnobotanical and filogenik then Coleus tuberosus 1b included in the group, which means the use of

Coleus tuberosus not only as food but also used in

disease treatment (Sunarjono, 2009). Coleus tuberosus is a crop of potential as an alternative

source of food carbohydrates. Some research

suggests that peel and flesh of Coleus tuberosus

contain bioactive compounds such ursolic acid, oleanolic acid, phenol and flavonoids, maslinic acid,

Phytosterol: stigmasterol, beta- sitosterol,

kampesterol (Mooi et al., 2010). Several methods were developed to determine the quality and quantity

of these compounds. The method used to measure the

antioxidant activity both fruit and vegetables and dairy products are DPPH (Kumaran et al., 2006), cell

culture models of cellular antioxidant activity (Wolfe

et al., 2008a; Wolfe et al., 2008b)

This study aims to determine the ability of the scavenging of free radicals with chemical tubes and in

biological systems. The method used by the chemical

of tube is 1.1-diphenyl-2-picrylhydrazyl (DPPH) and biological systems by the method of cellular

antioxidant activity based on the oxidation of 2 ', 7'-

dichlorofluorescein-diacetate (DCFH-DA) by reactive oxygen species (ROS) in Hela cancer cells. Use of the

method cellular antioxidant expected to be able to

describe the complexity of biological systems and is

an important tool to check out the food, phytochemicals and dietary supplements for potential

biological activity, because activity model cellular

antioxidant is considering making compound by the cell, distribution and efficiency of protection against

free radicals under physiological condition of the

body.

II. MATERIALS AND METHODS

2.1. Chemicals

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N. Baboby et al./1st International Conference Biodiversity Proceedings, 22-23 November 2016

Ethanol, 1.1-Diphenyl-2-picryl hydrazyl (DPPH),

Ethanolic extraxt peel of Coleus tuberosus (EEPC)

dan ethanolic extract flesh of Coleus tuberosus

(EEFC), RPMI, 2,7-diacetate dichlorofluorescein

(DCFH-DA), PMA from Sigma-Aldrich, Fetal

Bovine Serum (FBS) from Gibco. HeLa and T47D

were obtained from ATCC. All other reagents and

solvents were of analytical reagent grade.

2.2. Sample preparation:

The peel and flesh were separated by way of peeling the Coleus tuberosus, peel thickness approximately

1-1.5 mm. The peel and flesh then dried using a

cabinet drier at a temperature of 40oC during 24

hours. Then proceed with the process of milling and

filtered with sieve mesh size 80 tyler. The raw

material is then stored in a freezer (-20oC).

III. extraction process:

The peel and flesh of Coleus tuberosus flour were macerated with ethanol for 7 days (1:5), and

then filtered using whatman No. 1, ethanol

evaporated using N2 gas. Extract dissolved in 1 ml of

methanol, mixed and filtered with millex 0.45 µm.

IV. Determination of total phenolic contents

The concentration of phenolics in the ethanolic of

Coleus tuberosus was determined using spectrophotometric method (Singleton et al., 1999).

Methanolic solution of the extract in the

concentration of 1 mg/ml was used in the analysis.

The reaction mixture was prepared by mixing 0.5 ml of methanolic solution of extract, 2.5 ml of 10%

Folin- Ciocalteu’s reagent dissolved in water and 2.5

ml 7.5% NaHCO3. Blank was concomitantly prepared, containing 0.5 ml methanol, 2.5 ml 10%

Folin-Ciocalteu’s reagent dissolved in water and 2.5

ml of 7.5% of NaHCO3. The samples were thereafter incubated in a thermostat at 45 oC for 45 min. The

absorbance was determined using spectrophotometer

at λmax = 765 nm. The samples were prepared in

triplicate for each analysis and the mean value of absorbance was obtained. The same procedure was

repeated for the standard solution of gallic acid and

the calibration line was construed. Based on the measured absorbance, the concentration of phenolics

was read (mg/ml) from the calibration line; then the

content of phenolics in extracts was expressed in

terms of gallic acid equivalent (mg of GA/g of

extract)

V. Determination of flavonoids content

The content of flavonoids in the examined plant extracts was determined using spectrophotometric

method (Quettier et al., 2000). The sample contained

1 ml of methanol solution of the extract in the

concentration of 1 mg/ml and 1 ml of 2% AlCl3 solution dissolved in methanol. The samples were

incubated for an hour at room temperature. The

absorbance was determined using spectrophotometer at λmax = 415 nm. The samples were prepared in

triplicate for each analysis and the mean value of

absorbance was obtained. The same procedure was repeated for the standard solution of rutin and the

calibration line was construed. Based on the

measured absorbance, the concentration of

flavonoids was read (mg/ml) on the calibration line; then, the content of flavonoids in extracts was

expressed in terms of quercetin equivalent (mg of

quercetin/g of extract).

A. Determination of free radical scavenging activity

Evaluation by DPPH refers (Singh et al., 2009) 2 ml

of DPPH (0.1 mM in methanol) added with 300μL the peel or flesh extract of Coleus tuberosus (100,

200 and 400 μg/ml) in methanol, after 30 minutes

monitoring at λ 517nm. Ascorbic acid and BHT at concentration 10, 20 and 40 μg/ml used as a

control standard. Experiment three replicates.

IC50 values for determining the concentration

required to scavenging of 50% DPPH free radicals. B. Cell Culture

Human cervix cancer cells (HeLa) was obtained from

ATCC. Cells were cultured in the RPMI,

supplemented with 10% heat-inactivated Fetal Bovine

Serum and penicillin (100 units/ml-streptomycin

(100µg/ml), using 75 cm2 flasks in a 37oC in

humidified 5% CO2 incubator.

Cellular antioxidant activity in HeLa cancer cells. In this study the effect the flesh ethanolic extract flesh

of Coleus tuberosus (FEEC) and the peel ethanolic

extract of Coleus tuberosus (PEEC) on reduction of oxidative stress in HeLa cells were evaluated on

cellular antioxidant. The basis of the method reported

by Chang et al. (2001), 2-,7-Dichlorofluorescin diacetate (DCFH-DA), a peroxide-sensitive dye, was

used for the evaluation of oxidative stress in cells

based on oxidation of DCFH-DA by Reactive Oxygen

Species. In this study, HeLa cells were cultured in RPMI supplemented with 10% fetal bovine serum

(FBS), 100 units/mL penicillin, and streptomycin in an

incubator at 37 °C, 5% CO2, 95% air humidity. The

N. Baboby et al./1st International Conference Biodiversity Proceedings, 22-23 November 2016

4

cell suspensions (200 µl at the concentration of 105

cells/well) were seeded in and incubated with EEFC

and EEPC (100, 200, 400 and 800 µg/ml) for 20

min. Then cells were co-incubated with 25 µM

DCFH-DA in the absence or presence of 100 ng PMA in darkness at 37 °C for 30 min. After

incubation, cells were collected and washed once

with ice-cold phosphatebuffered saline (PBS), resuspended in 200 µl of the same PBS, and placed

on ice in darkness until flow cytometry was carried

out. The amounts of intracellular hydrogen peroxide were detected by BD flow cytometer. At least 10000

cells were analyzed for each test, and the observed

fluorescence reflects the intracellular hydrogen

peroxide level.

In this test, oxidative stress is induced by

addition of PMA in the extracellular medium of the

HeLa cancer cells. The antioxidant activity express

on the reduction percentage of ROS generated in

HeLa by exogenous PMA was calculated by the

monitoring of the emitted fluorescence intensity (Fi).

The following relation was used

(Fito − Fit1) × 100/(Fito− Fit2)

with Fito: control with oxidative stress; Fit1: treat

cells; Fit2: control without oxidative stress (Muanda

et al., 2011).

III. RESULTS AND DISCUSSION

A. Phenol and flavonoid

Extracting part of the peel and the flesh of the Coleus

tuberosus using ethanol analyzed levels of phenols

and flavonoids. Table 1 shows that the peel contains phenols and flavonoids higher than the flesh. This is

in line with some research that shows that the peel

has higher bioactive compounds compared parts of

the flesh (Nurliyana et al., 2010; Wolfe et al., 2003).

Table 1. Phenol and flavonoid content on the

ethanolic extract of peel and flesh of Coleus

tuberosus

Part of tuber

Phenol (mg of GA/g of

extract)

Flavonoid (mg of quercetin/g

of extract)

Peel 6.10 ± 0.02 3.08 ± 0.05

Flesh 1.77 ± 0.01 0.26 ± 0.01

A. The antioxidant activity with DPPH method

Antioxidant activity could be tested by

measuring the power free radical scavenging.

Radical DPPH method using synthetic 1,1,-picryl

hydrazyl diphenyl (DPPH). DPPH easily and thoroughly enough, then the DPPH is currently

widely used for the measurement of free- radical-

scavenging (Singh et al., 2009). The peel and flesh

ethanol extracts of Coleus tuberosus measured in the hydrogen donates its ability or scavenging the radical

using free radical 1.1-diphenylpricrylhydrazyl

(DPPH).

The ability of antioxidants with DPPH method

on the peel ethanolic extract of Coleus tuberosus

(PEC) and the flesh ethanolic extract of Coleus

tuberosus (FEC) indicated by the IC50 (Table 2).

Inhibitory concentration 50 (IC50) demonstrated

ability to scavenging free radicals (DPPH) by 50%,

the smaller the IC50 showed higher antioxidant

activity. Based on the IC50 ethanolic extract peel of

Coleus tuberosus in this study is higher than in the

flesh.

Tabel 2. IC50 the ethanolic extract peel and

flesh of Coleus tuberosus with DPPH method

Note: different notation means significant different p < 0.05.

This study proved that the antioxidant activity

(IC50) ethanolic extract peel is higher than ethanolic extract flesh of Coleus tuberosus. The difference in

antioxidant activity on the part of the flesh and the

peel caused by bioactive compounds such as phenolic acid, flavonoid. Phenol compounds have

the ability to scavenging free radicals, it is evidenced

by the strong correlation between the content of

phenolic compounds and radical scavenging activity (O’Sullivan et al., 2011; Jung et al., 2011). The

activity of scavenging free radicals is determined by

the number of protons available for transfusion by the hydroxyl group and the structure of phenolic

hydroxyl group on the benzene ring contribute to

arrest free radicals (DPPH) (Fan et al., 2011).

The difference in antioxidant activity between

PEC and FEC based on DPPH method thought to be

caused by differences in the content of bioactive

compound (Table1). The peel contains more bioactive compounds have a greater ability to transfer hydrogen

atoms to free radicals (DPPH), so that the formation of

diphenyl picrylhydrazyl compound higher than in the

Compound IC50 (µg/ml)

The flesh of ethanolic extract of Coleus

tuberosus (FEC) 1290.00±1.58a

The peel of ethanolic extract of Coleus

tuberosus (PEC) 310.97±0.32b

BHT 50.12±0.53c

Ascorbic acid 19.28±0.15d

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N. Baboby et al./1st International Conference Biodiversity Proceedings, 22-23 November 2016

flesh. The greater the picrylhydrazyldiphenyl

compound formed showed greater antioxidant ability, especially scavenging free radicals. Research

shows that the difference between the antioxidant

activity of the peel and flesh of the fruit and

vegetables caused by difference content of bioactive compounds (Nurliyana et al., 2010; Wolfe et al.,

2003).

B. Cellular antioxidant activity

Principle Celullar Antioxidant Activity/CAA is

knowing the antioxidant activity of a compound by

measuring its ability to inhibit the oxidation of 2 .7 '-

' dichlorfluorescein-diacetate (DCFH-DA) fluorescence 2 ' 7 '-dichlorofluorescin (DCF) by ROS

on cell culture. Cellular antioxidant activity in HeLa

cells by treatment ethanolic extract peel and flesh of

Coleus tuberosus in Figure 1.

Fig 1 (A, B) show that the peel and flesh

ethanolic extract of Coleus tuberosus able to reduce

the formation of reactive oxygen species (ROS) in HeLa cells induced by PMA dose dependent manner.

Part of the peel have the ability reduces ROS higher

than the flesh of Coleus tuberosus. This is related to the difference of bioactive compounds in the peel and

the flesh. Part of the peel contain phenol, flavonoids

higher flesh of the ethanolic extract of peel and flesh

of Coleus tuberosus (Table 1).

Decrease of ROS in HeLa cells by the peel

and flesh ethanolic extract of Coleus tuberosus

allegedly through some mechanism, namely the ability of scavenging free radicals attack cell

membrane and maintain the fluidity of cell

membrane, repair and increase the antioxidant defense system (enzymatic reactions and non-

enzymatic reactions).

Reactive oxygen species reduction mechanism in the peel and flesh ethanolic extract of Coleus tuberosus

expected as mechanism of bioactive compounds

contained it that scavenging the ROS attack the cell

membrane. Increased ROS in the cells causing the cell membrane lipid undergoes oxidation so that the cell

membrane permeability and fluidity changes. Phenolic

compounds have the ability to maintain the fluidity of cell membranes to capture ROS, so the cellular

communication signal level can run well including

signal activation of antioxidant enzymes (NRF-2-

ARE). Another mechanism underlying the antioxidative properties of phenolics is the ability of

flavonoids to alter peroxidation kinetics by

modification of the lipid packing order and to decrease fluidity of the membranes (Arora et al., 2000). These

changes could sterically hinder diffusion of free radicals

and restrict peroxidative reactions.

Increased expression of NRF-2-ARE

increasing role in the cell's antioxidant defense

system (SOD, CAT, GPx, glutathione, vitamin C, vitamin E and carotene). An increase in the cell's

antioxidant defense system (SOD, CAT, GPx) give

effect to the increased ability to neutralize

superoxide anion radicals (O2• -), singlet oxygen

(1O2), hydrogen peroxide (H2O2) and hydroxyl

radical (•OH) induced by PMA, so as to decrease the

number of free radicals in the HeLa cells.

The peel and the flesh ethanolic extract of

Coleus tuberosus also have some kind of bioactive

compounds such as phenolic and flavonoid compounds (Table 1). Phenol compounds have the

ability to increase the antioxidant defense system

(Giovanini et al., 2008; Verma et al., 2009). Increased

cellular antioxidant activity may prevent 2.7 dichlorofluorescein diacetate-(DCFH) hydrocarbon

and reduce the formation of 2.7-dichlorofluorescein

diacetate fluorescent DCF (Salawu et. Al., 2011).

This study proves that the ethanolic extract peel

of Coleus tuberosus able to reduce ROS is greater than

the ethanol extract flesh of Coleus tuberosus.

Differences in the ability to reduce ROS, one of them allegedly associated with differences in the content of

phenol, flavonoids (Table1).

N. Baboby et al./1st International Conference Biodiversity Proceedings, 22-23 November 2016

6

A

B

Figure 1. Percentage reduction in reactive oxygen

species (ROS) by treatment PEC (A) and FEC (B) in HeLa cells induced by Phorbol

Miristate Acetate

Note: different notations indicate significant difference (p <0.05).

Some research has also shown that the peel has

the ability of antioxidants that are higher than the portion of flesh. This difference is associated with the

bioactive compounds in the peel section is higher

than the portion of flesh. The antioxidant activity of apple peel part higher than the flesh, this difference

was associated with a significant positive correlation

between the content of anthocyanin, flavonoids, phenol, with antioxidant activity (Vieira et al., 2009).

The antioxidant activity of the peel of apple fruit is

higher than the flesh. Comparative evaluation of

antioxidant with the DPPH method and cellular antioxidant activity showed that the DPPH method

(in vitro chemical) similar tendency, where the once

the antioxidant activity of ethanolic extract of peel the Coleus tuberosus greater than the flesh ethanolic

extract of Coleus tuberosus.

Evaluation of antioxidant activity in vitro

chemical (DPPH), the reaction tends to be on antioxidant compounds tested and free radicals are

added. So the ability to scavenging of free radicals is

highly dependent on the number of OH groups in its structure. While the evaluation of antioxidant

activity using a biological system in this case is the

HeLa cells, the antioxidant ability of a compound is not only a reaction between antioxidant compounds

were tested by free radical compounds (PMA), but

also involves other cellular mechanisms in the cell

such as the cell membrane fluidity, antioxidant

defense system.

Thus, in vitro biological system evaluation in

cells can describe the complexity of biological

systems and is an important tool to check the food,

phytochemicals and dietary supplements potential for biological activity, because the model considers the

cellular antioxidant activity of the compound by the

cell retrieval, distribution and efficiency protection against free radicals under physiological conditions.

This study proves that the bioactive compounds that

ursolic acid, oleanolic acid, maslinic acid, flavonoids, phenolic and phytosterol compounds contribute to

the antioxidant activity of the peel and flesh ethanolic

extract Coleus tuberosus. A decrease in oxidative

stress in cells may provide a positive effect on its potency in a biological system, especially the anti-

proliferation of cancer cells.

Evaluation of antioxidant activity for screening free radical scavenging ability of a

compound can be done by combining the two

methods, in vitro chemical (DPPH) and in vitro in

biological systems (cell). This is done to provide a clear picture of the antioxidant potential of a

compound. Variations in having antioxidant method

in strengthening the information obtained. This research is expected to provide information, that

Coleus tuberosus as a vegetable is not only a source

of carbohydrates but also have potential as natural

antioxidants.

ACKNOWLEDGEMENTS

Authors thank to the Directorate General of Higher

Education Republic of Indonesia that has funded this

research.

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