of the Available Data on the Biolo g · Table G-1. A Summar y of the Available Data on the Biolo g...

Table G-1. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Arsenic in the St. Louis River Area of Concern

Arsenic (mg/kg) Area

Test Duration

Test Organism Species Life Stage

End-Point Measured Result

TOC (%) Reference

<1.27 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 1996<1.27 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 1996<1.27 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 1996<1.27 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 1996<1.52 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996<1.52 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996<1.52 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996<1.52 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996<1.62 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 1996<1.62 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 1996<1.62 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 1996<1.62 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 1996<2.04 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996<2.04 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996<2.04 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996<2.04 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996<2.15 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 1996<2.15 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 1996<2.15 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 1996<2.15 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 1996<2.20 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 1996<2.20 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 1996<2.20 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 1996<2.20 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 1996<2.54 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 1996<2.54 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 1996<2.54 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 1996<2.54 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 1996<2.84 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 1996<2.84 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 1996

G-3



Test Duration



TOC (%) Reference

<2.84 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 1996<2.84 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 1996<3.45 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 1996<3.45 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 1996<3.45 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 1996<3.45 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 19963.70 D-S Harbor - NW of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 19953.70 D-S Harbor - NW of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia Adult 100 % survival Not toxic 8.4 Wenck Associates Inc. 19953.70 D-S Harbor - NW of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 19953.70 D-S Harbor - NW of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 1995

<4.00 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 1996<4.00 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 1996<4.00 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 1996<4.00 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 19964.40 D-S Harbor - Hallett Coal Dock #6 48 hour Cladoceran Daphnia magna Unknown 95 % survival Not toxic NR Smith et al. 19924.40 D-S Harbor - NE of Sanitary District Plant 48 hour Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR Smith et al. 19924.40 D-S Harbor - NE of Sanitary District Plant 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 19924.40 D-S Harbor - Hallett Coal Dock #6 10 day Diptera Chironomus tentans Unknown 86.6 % survival Not toxic NR Smith et al. 19924.60 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia Adult 100 % survival Not toxic 6.7 Wenck Associates Inc. 19954.60 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 19954.60 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 19954.60 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 19954.80 D-S Harbor - North of MN Power & Light Dock 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 19924.80 D-S Harbor - North of MN Power & Light Dock 48 hour Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR Smith et al. 19925.40 D-S Harbor - Allouez Bay 48 hour Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR Smith et al. 19925.40 D-S Harbor - Allouez Bay 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 19928.21 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic NR Crane et al. 19978.21 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19978.70 Brule River - above Stones Bridge 48 hour Cladoceran Daphnia magna Unknown 95 % survival Not toxic NR Smith et al. 19928.70 Brule River - above Stones Bridge 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 1992

G-4



Test Duration



TOC (%) Reference

14.2 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 199714.2 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic NR Crane et al. 199717.7 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199717.7 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic NR Crane et al. 199719.0 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 199319.3 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 199719.3 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic NR Crane et al. 199720.2 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 199320.5 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997

<21.1 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 1996<21.1 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 1996<21.1 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 1996<21.1 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 199621.6 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 199323.1 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 199723.1 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 199724.3 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 199725.0 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 199625.0 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 199625.0 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 199625.0 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 199627.5 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic NR Crane et al. 199727.5 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199728.1 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 199628.1 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 199628.1 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 199628.1 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 199630.9 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199630.9 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199630.9 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 1996

G-5



Test Duration



TOC (%) Reference

30.9 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199639.7 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 199639.7 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 199639.7 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 199639.7 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 1996

D-S = Duluth-Superior; LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-6

Table G-2. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Cadmium in the St. Louis River Area of Concern

Cadmium(mg/kg) Area Test Duration

Test Organism Species Life Stage End-Point Measured Result

TOC (%)

0.020 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.080.020 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.080.020 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.080.020 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.080.110* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.280.110* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 58 % survival Toxic 0.280.124* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.160.124* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 red. biolum.^ Toxic 0.160.124* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.160.141* Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.350.141* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.350.141* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 red. biolum.^ Toxic 0.350.150* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 0.890.150* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 0.890.177* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.690.177* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 1.690.177* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.690.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.40.200* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 red. biolum.^ Not toxic 0.120.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.40.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.40.200* St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.120.200* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.120.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 red. biolum.^ Toxic 0.40.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.40.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.080.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.080.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.080.200 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.640.200 St. Louis River-Keene Creek 10 day Amphipod Chironomus tentans JUV 92 % survival Not toxic 4.640.200 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.640.200 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.640.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.240.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.240.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.240.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2

0.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4

G-7




TOC (%)

0.200* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.590.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 red. biolum.^ Toxic 0.140.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.140.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.20.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.140.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 red. biolum.^ Toxic 0.20.200* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 0.590.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.20.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.140.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 red. biolum.^ Toxic 0.080.200* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 0.140.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 45.3 red. biolum.^ Toxic 0.140.200* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 9.08 EC50 biolum.# Not toxic 0.140.200* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 64 % survival Not toxic 0.140.200* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.590.201* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 0.860.201* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 14.18 EC50 biolum.# Not toxic 0.860.201* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 0.860.201* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 0.860.204* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 red. biolum.^ Not toxic 1.370.204* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.370.204* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.370.204* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.370.204* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.370.217* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic 0.810.217* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 0.810.217* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.9 red. biolum.^ Toxic 0.810.217* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.06 EC50 biolum.# Not toxic 0.810.243* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.650.243* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.650.243* Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.650.243* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.650.243* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.650.249* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.980.249* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98

0.249* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 red. biolum.^ Not toxic 2.980.260* Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47

G-8




TOC (%)

0.260* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 red. biolum.^ Toxic 4.470.260* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.470.260* Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.470.263* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.440.263* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 red. biolum.^ Not toxic 1.440.263* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.440.287* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 red. biolum.^ Not toxic 4.020.287* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.020.287* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.020.287* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.020.287* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.020.288* St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 0.560.288* St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 0.560.288* St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.12 EC50 biolum.# Not toxic 0.560.288* St. Louis River 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.560.308* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 red. biolum.^ Toxic 1.020.308* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.020.308* Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.020.308* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.020.310 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.70.310 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.70.310 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.70.310 Superior Bay 48-h Cladoceran Ceriodaphnia dubia Adult 100 % survival Not toxic 6.70.324* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.610.324* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 5.36 EC50 biolum.# Not toxic 0.610.324* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 0.610.324* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 0.610.326* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 1.850.326* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.75 EC50 biolum.# Not toxic 1.850.326* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 14.9 red. biolum.^ Not toxic 1.850.326* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 1.850.335* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.770.335* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.770.335* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77

0.335* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 red. biolum.^ Not toxic 5.770.335* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 1.710.335* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71

G-9




TOC (%)

0.335* Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.710.335* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.710.335* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.710.356* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 red. biolum.^ Not toxic 1.820.356* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.820.356* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.820.356* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.820.388* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.070.388* Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.070.388* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 red. biolum.^ Toxic 3.070.409* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic NR0.409* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 12 % survival Toxic NR0.409* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 60 % survival Not toxic NR0.409* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.4 EC50 biolum.# Not toxic NR0.424* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.70.424* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.70.424* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.70.424* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.70.424* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 2.70.424* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.70.424* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.70.424* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.70.424* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 red. biolum.^ Not toxic 2.70.424* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.70.440 Duluth-Superior Harbor-Hallett Coal Dock #6 10 day Diptera Chironomus tentans Unknown 86.6 % survival Not toxic NR0.440 Duluth-Superior Harbor-Hallett Coal Dock #6 48-h Cladoceran Daphnia magna Unknown 95 % survival Not toxic NR0.450 Duluth-Superior Harbor-Northwest side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.40.450 Duluth-Superior Harbor-Northwest side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.40.450 Duluth-Superior Harbor-Northwest side of Lakehead Dock 48-h Cladoceran Ceriodaphnia dubia Adult 100 % survival Not toxic 8.40.450 Duluth-Superior Harbor-Northwest side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.40.450* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.40.450* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.40.450* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.46 EC50 biolum.# Not toxic 2.4

0.450* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 35.18 red. biolum.^ Toxic 2.40.469* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 red. biolum.^ Toxic 10.469* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 10.469* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1

G-10




TOC (%)

0.469* Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 10.469* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 10.502* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.590.502* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.590.502* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.590.502* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 2.590.511* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.680.511* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.83 EC50 biolum.# Not toxic 0.680.511* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 21.31 red. biolum.^ Toxic 0.680.514* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 1.750.514* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.87 red. biolum.^ Toxic 1.750.514* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.750.514* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 1.750.530* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.80.530* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 1.80.532* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.820.532* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 red. biolum.^ Toxic 0.820.532* Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.820.532* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.820.555* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 2.20.555* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.20.555* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.31 red. biolum.^ Not toxic 2.20.555* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.20.566* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.1 EC50 biolum.# Not toxic 3.20.566* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 45.6 red. biolum.^ Toxic 3.20.566* Spirit Lake 10 day Diptera Chironomus tentans LAR 50 % survival Not toxic 3.20.570 Duluth-Superior Harbor-Allouez Bay 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR0.570 Duluth-Superior Harbor-Allouez Bay 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR0.590 Duluth-Superior Harbor-North of Minnesota 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR0.590 Duluth-Superior Harbor-North of Minnesota

i h k10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR

0.593* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.020.593* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 3.02

0.593* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.020.593* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.020.599* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.23 EC50 biolum.# Toxic 2.820.599* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 3.17 red. biolum.^ Not toxic 2.820.599* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.82

G-11




TOC (%)

0.599* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 2.820.608* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 27.4 red. biolum.^ Toxic 2.190.608* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 4.18 EC50 biolum.# Not toxic 2.190.608* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.190.608* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.190.612* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.820.612* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.820.612* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 red. biolum.^ Not toxic 2.820.612* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.820.630* St. Louis Bay 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 1.550.630* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.7 red. biolum.^ Not toxic 1.550.630* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 1.550.637* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.240.637* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.240.637* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.240.637* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.240.637* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.240.656* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.10.656* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 1.10.656* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.10.656* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.10.662* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.690.662* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 1 red. biolum.^ Not toxic 3.690.662* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.690.662* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.690.668* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 2.340.668* Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.340.668* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.340.668* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.340.668* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.340.672* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.94

0.672* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 40.37 red. biolum.^ Toxic 1.940.672* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 1.940.672* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.940.673* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.910.673* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.910.673* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 red. biolum.^ Not toxic 2.91

G-12




TOC (%)

0.673* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.910.677* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 red. biolum.^ Toxic 1.870.677* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.870.677* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.870.677* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.870.677* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.870.679* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 8.40.679* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.40.679* Spirit Lake 10 day Amphipod Hyalella azteca JUV 3 % survival Toxic 8.40.679* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 81.5 red. biolum.^ Toxic 8.40.679* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.850.679* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.850.679* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.850.679* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.850.679* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.850.681* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 3.20.681* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.20.681* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 3.20.681* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.20.688* St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.720.688* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.720.688* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.720.688* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.720.688* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 6.720.695* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 28.9 red. biolum.^ Toxic 2.370.695* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.370.695* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 2.370.695* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.64 EC50 biolum.# Not toxic 2.370.700* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 7.10.700* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 7.1

0.725* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 red. biolum.^ Not toxic 2.430.725* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.430.725* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.430.740* St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.360.740* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.360.740* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 red. biolum.^ Not toxic 4.360.740* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36

G-13




TOC (%)

0.740* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 2.90.740* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.90.745* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.340.745* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.340.745* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.28 EC50 biolum.# Toxic 2.340.745* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.82 red. biolum.^ Not toxic 2.340.750* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.810.750* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 red. biolum.^ Not toxic 13.810.750* Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.810.750* Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.810.757* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.40.757* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.40.757* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 red. biolum.^ Not toxic 3.40.757* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.40.757* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.40.757* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 red. biolum.^ Not toxic 3.40.757* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.40.757* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.40.757* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.40.757* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.40.766* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.250.766* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 red. biolum.^ Toxic 3.250.766* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.250.790* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.60.790* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.60.802* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.7350.802* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.7350.802* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.7350.802* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.735

0.802* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.7350.820* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.30.820* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 2.30.850* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 2.50.850* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Toxic 2.50.889* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.670.889* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 31.2 red. biolum.^ Toxic 2.670.889* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 91 % survival Not toxic 2.67

G-14




TOC (%)

0.889* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 2.670.890* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.90.890* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 2.90.900* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 3.320.900* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.1 red. biolum.^ Not toxic 3.320.900* Spirit Lake 10 day Amphipod Hyalella azteca JUV 55 % survival Not toxic 3.320.900* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 3.320.905* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 red. biolum.^ Toxic 13.910.905* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.910.905* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.910.905* St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.910.905* St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.910.913* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.840.913* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 red. biolum.^ Not toxic 4.840.913* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.840.913* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.840.920* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.140.920* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.140.920* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.4 red. biolum.^ Toxic 3.140.921* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.970.921* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.970.921* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.970.921* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.970.921* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 8.970.924* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.350.924* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.350.924* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.350.924* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35

0.938* St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.630.938* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.630.938* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 red. biolum.^ Toxic 2.630.938* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.630.940* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.420.940* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.420.940* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 red. biolum.^ Toxic 1.420.940* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.420.942* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 3.07

G-15




TOC (%)

0.942* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.070.942* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 3.070.942* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.070.964* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 35 % survival Not toxic 3.380.964* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.380.964* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 19.2 red. biolum.^ Not toxic 3.380.964* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 3.380.970* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.410.970* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 4.410.970* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.410.970* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.3 EC50 biolum.# Toxic 4.410.970* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 120.970* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 120.999* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.970.999* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.970.999* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.970.999* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.970.999* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.971.01 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.41.01 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.41.01 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.41.01* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 3.91.01* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 3.91.01 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.41.01* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.341.01* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 red. biolum.^ Not toxic 5.341.01* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34

1.01* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.341.01* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.931.01* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 13.8 red. biolum.^ Not toxic 2.931.01* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 2.931.02* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.131.02* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.131.02* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.131.02* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 red. biolum.^ Toxic 4.131.03* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.241.03* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.12 EC50 biolum.# Toxic 4.24

G-16




TOC (%)

1.03* Spirit Lake 10 day Amphipod Hyalella azteca JUV 41 % survival Toxic 4.241.03* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 53.2 red. biolum.^ Toxic 4.241.04* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.991.04* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.991.04* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.991.04* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.771.04* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.771.04* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 red. biolum.^ Toxic 6.771.04* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.771.04* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.771.04* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 3.991.04* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 10 % survival Toxic 3.991.04* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 66.5 red. biolum.^ Toxic 3.991.04* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 3.991.04* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.951.04* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.951.04* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.951.04* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 red. biolum.^ Toxic 3.951.04* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 red. biolum.^ Toxic 3.951.04* Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.951.05* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.051.05* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.051.05* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.051.05* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 red. biolum.^ Toxic 4.051.05* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.051.07* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.57 EC50 biolum.# Not toxic 2.37

1.07* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 77.5 % survival Not toxic 2.371.07* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.22 red. biolum.^ Not toxic 2.371.07* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.371.08* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 31.08* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 31.10* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.981.10* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 red. biolum.^ Toxic 13.981.10* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.981.10* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.981.13* St. Louis Bay 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.881.13* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.45 EC50 biolum.# Toxic 2.88

G-17




TOC (%)

1.13* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.881.13* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 red. biolum.^ Toxic 8.261.13* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.261.13* Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.261.13* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.261.14* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.981.14* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.981.14* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.981.14* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 red. biolum.^ Not toxic 3.981.15* St. Louis Bay 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.021.15* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.021.15* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 4.021.15* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 4.021.17* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 2.81.21 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.421.21* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic 31.21 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.421.21 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.421.21 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.421.21* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.871.21* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic 3.871.21* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.871.21* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.871.21* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.871.22 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76

1.22 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.761.22 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.761.22 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.761.27* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.111.27* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.111.27* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 4.111.27* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.111.27* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.111.27* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.111.28* St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.471.28* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.471.28* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47

G-18




TOC (%)

1.28* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 red. biolum.^ Toxic 3.471.29* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 6.51.29* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 6.51.32* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.21.32* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic 2.21.34* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 5.21.34* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 5.21.39* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 51.39* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 51.41 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.161.41 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.161.41 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.161.41 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.161.42* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.9751.42* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.9751.42* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.9751.42* Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.9751.42* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 red. biolum.^ Toxic 11.9751.42* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 10.681.42* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 10.681.42* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 14.8 red. biolum.^ Not toxic 10.681.42* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.051.42* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.051.42* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 red. biolum.^ Not toxic 4.05

1.42* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.051.44 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.931.44 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.931.44 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.931.44 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.931.45* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 44 % survival Not toxic 3.761.45* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 11.1 red. biolum.^ Not toxic 3.761.45* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 3.761.45* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.25 EC50 biolum.# Toxic 3.761.46* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.61.46* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic 6.61.51* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.21.51* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.2

G-19




TOC (%)

1.54* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 red. biolum.^ Not toxic 5.591.54* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.591.54* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.591.54* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.591.56* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.541.56* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.541.56* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 red. biolum.^ Toxic 4.541.56* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.541.57* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 red. biolum.^ Toxic 2.131.57* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.131.57* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.131.57* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.131.62* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.031.62* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 red. biolum.^ Toxic 6.031.62* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.031.67* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 red. biolum.^ Not toxic 4.71.67* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.71.67* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.71.67* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.71.70 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.151.70 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.151.70 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.151.70 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15

1.89* St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.841.89* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.841.89* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.841.89* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.841.89* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 29.841.97* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.481.97* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.481.97* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 red. biolum.^ Toxic 4.482.00 Duluth-Superior Harbor-NW of Western Lake Superior 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR2.00 Duluth-Superior Harbor-NW of Western Lake Superior 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR2.27* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.492.27* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 17.492.27* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.492.27* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49

G-20




TOC (%)

2.57 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.882.66* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.192.66* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.192.66* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.192.66* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 red. biolum.^ Toxic 14.192.66 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.122.82 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.12.82 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.12.82 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.12.82 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.12.86 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.122.86 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.122.86 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.122.86 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.123.01 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.583.57 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.373.57 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.373.57 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.373.57 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.374.26 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.954.26 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.954.26 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95

4.26 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.954.68 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR4.68 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR4.68 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR4.68 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR6.13 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.546.13 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.546.13 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.546.13 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.547.00 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic NR7.00 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR7.00 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic NR7.00 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic NR

* = SEM measurement; ^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; red. Biolum. = reduced bioluminescence; EC50 = effective concentration affecting 50% of the population.

G-21




TOC (%)

LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-22

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

MPCA 1997b

G-23

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

MPCA 1997cMPCA 1997b

G-24

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

MPCA 1997bMPCA 1997cMPCA 1997c

G-25

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cSmith et al. 1992Smith et al. 1992Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997b

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997c

G-26

Reference

MPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bSmith et al. 1992Smith et al. 1992Smith et al. 1992Smith et al. 1992MPCA 1997bMPCA 1997b

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-27

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997b

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-28

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-29

Reference

Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

MPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997b

G-30

Reference

MPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-31

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997b

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-32

Reference

MPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997b

MPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-33

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997IT Corp. 1996Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996

IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-34

Reference

MPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

MPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997

G-35

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cSmith et al. 1992Smith et al. 1992MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-36

Reference

MPCA 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-37

Reference

G-38

Table G-3. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Chromium in the St. Louis River Area of Concern

Chromium (mg/kg) Area

Test Duration



TOC (%) Reference

3.10 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19963.10 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 19963.10 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 19963.10 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 19964.50 St. Louis River - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 19964.50 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 19964.50 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 19964.50 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 199622.9 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 199622.9 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 199622.9 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 199622.9 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 199630.5 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 199630.5 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 199630.5 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 199630.5 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 199639.0 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 199539.0 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4 Wenck Associates Inc. 199539.0 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 199539.0 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 199541.2 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199641.2 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 199641.2 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199641.2 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199645.5 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 199645.5 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 199645.5 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 199645.5 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 199647.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 199547.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 199547.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 199547.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995

G-23



Test Duration



TOC (%) Reference

50.5 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 199650.5 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 199650.5 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 199650.5 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 199651.3 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 199651.3 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 199651.3 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 199651.3 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 199667.6 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 199374.0 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 199674.0 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 199674.0 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 199674.0 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 199686.7 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 199686.7 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 199686.7 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 199686.7 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 199688.3 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199688.3 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 199688.3 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199688.3 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199690.5 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 199690.5 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 199690.5 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 199690.5 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996104 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 1996104 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 1996104 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 1996104 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 1996111 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 1993207 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993216 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 1996

G-24



Test Duration



TOC (%) Reference

216 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 1996216 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 1996216 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 1996247 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 1996247 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 1996247 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 1996247 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 1996

* = SEM measurement; ^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-25

Table G-4. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Lead in the St. Louis River Area of Concern

Lead (mg/kg) Area

Test Duration

Test Organism Species

Life Stage


TOC (%)

1.17* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.83 EC50 biolum.# Not toxic 0.681.17* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 21.31 red. biolum.^ Toxic 0.681.17* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.681.20* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 red. biolum.^ Toxic 0.081.20* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.081.20* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.081.20* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.081.80* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 0.861.80* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 0.861.80* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 0.861.80* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 14.18 EC50 biolum.# Not toxic 0.861.85* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.591.85* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.591.85* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 0.592.17* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.352.17* Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.352.17* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 red. biolum.^ Toxic 0.352.20* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.42.20* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.42.20* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.42.20* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.42.20* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 red. biolum.^ Toxic 0.42.20* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.42.23* Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.022.23* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.022.23* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.022.23* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 red. biolum.^ Toxic 1.022.26* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.562.26* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 0.562.26* St. Louis River-Lower St. Louis River 30 minute Bacteria Chironomus tentans NA 12 red. biolum.^ Not toxic 0.56

G-26


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

2.26* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.12 EC50 biolum.# Not toxic 0.562.29* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.282.29* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 58 % survival Toxic 0.282.48* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 red. biolum.^ Toxic 0.162.48* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.162.48* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.162.60* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.22.60* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.22.60* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 red. biolum.^ Toxic 0.22.60* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.22.72* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.372.72* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.372.72* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.372.72* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.372.72* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 red. biolum.^ Not toxic 1.372.88* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.122.88* St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.122.88* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.122.88* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 red. biolum.^ Not toxic 0.123.05* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 0.613.05* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 0.613.05* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.613.05* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 5.36 EC50 biolum.# Not toxic 0.613.17* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 45.3 red. biolum.^ Toxic 0.143.17* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 64 % survival Not toxic 0.143.17* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 9.08 EC50 biolum.# Not toxic 0.143.17* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 0.143.71* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 4.18 EC50 biolum.# Not toxic 2.193.71* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 27.4 red. biolum.^ Toxic 2.193.71* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.19

G-27


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

3.71* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.193.72* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.243.72* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.243.72* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 red. biolum.^ Toxic 0.243.72* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.243.87* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 0.893.87* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 0.894.05* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 1.14.05* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.14.05* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.14.05* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.14.58* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.144.58* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 red. biolum.^ Toxic 0.144.58* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.144.58* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.144.79* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.424.79* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.424.79* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.424.79* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 red. biolum.^ Toxic 1.424.90* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.74.90* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.74.90* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.74.90* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.74.90* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 2.74.90* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.74.90* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 red. biolum.^ Not toxic 2.74.90* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.74.90* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.74.90* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.74.96* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82

G-28


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

4.96* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 red. biolum.^ Not toxic 1.824.96* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.824.96* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.825.37* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.46 EC50 biolum.# Not toxic 2.45.37* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.45.37* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 35.18 red. biolum.^ Toxic 2.45.37* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.45.40* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 8.45.40* Spirit Lake 10 day Amphipod Hyalella azteca JUV 3 % survival Toxic 8.45.40* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 81.5 red. biolum.^ Toxic 8.45.40* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.45.64* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 2.25.64* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.25.64* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.25.64* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.31 red. biolum.^ Not toxic 2.25.65* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.695.65* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.695.65* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 1.695.69* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.87 red. biolum.^ Toxic 1.755.69* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 1.755.69* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 1.755.69* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.755.74* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.875.74* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.875.74* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.875.74* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 red. biolum.^ Toxic 1.875.74* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.875.96* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.655.96* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.655.96* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65

G-29


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

5.96* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.655.96* Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.656.01* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.356.01* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.356.01* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.356.01* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.356.31* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.446.31* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 red. biolum.^ Not toxic 1.446.31* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.446.36* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 3.17 red. biolum.^ Not toxic 2.826.36* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 2.826.36* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.826.36* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.23 EC50 biolum.# Toxic 2.826.43* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 3.026.43* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.026.43* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.026.43* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.026.58* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 7.16.58* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 7.16.67* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic NR6.67* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.4 EC50 biolum.# Not toxic NR6.67* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 60 % survival Not toxic NR6.67* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 12 % survival Toxic NR6.68* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.476.68* Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.476.68* Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.476.68* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 red. biolum.^ Toxic 4.476.78* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.916.78* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.916.78* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 red. biolum.^ Not toxic 2.91

G-30


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

6.78* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.916.82* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 1.716.82* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.716.82* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.716.82* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.716.82* Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.717.03* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 1.857.03* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 1.857.03* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 14.9 red. biolum.^ Not toxic 1.857.03* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.75 EC50 biolum.# Not toxic 1.857.06* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.777.06* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 red. biolum.^ Not toxic 5.777.06* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.777.06* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.777.27* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.377.27* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 2.377.27* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 28.9 red. biolum.^ Toxic 2.377.27* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.64 EC50 biolum.# Not toxic 2.377.64* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.247.64* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.247.64* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.247.64* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.247.64* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.247.94* St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.4 red. biolum.^ Toxic 3.147.94* St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.147.94* St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.148.47* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.828.47* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.828.47* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.828.47* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 red. biolum.^ Not toxic 2.82

G-31


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

8.47* Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.078.47* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 red. biolum.^ Toxic 3.078.47* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.079.48* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 red. biolum.^ Toxic 3.259.48* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.259.48* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.259.78 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.939.78 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.939.78 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.939.78 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.939.90* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.7 red. biolum.^ Not toxic 1.559.90* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 1.559.90* St. Louis Bay 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 1.559.95* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 red. biolum.^ Not toxic 2.989.95* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.989.95* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.989.95* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.989.95* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.9810.6* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.8510.6* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.8510.6* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.8510.6* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.8510.6* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.8510.7 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.6410.7 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.6410.7 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.6410.7 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.6410.9* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic 0.8110.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.9 red. biolum.^ Toxic 0.8110.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.06 EC50 biolum.# Not toxic 0.81

G-32


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

10.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 0.8111.1* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.5911.1* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 2.5911.1* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.5911.1* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.5911.1* Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.2611.1* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.2611.1* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.2611.1* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 red. biolum.^ Toxic 8.2611.7* St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.7211.7* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 6.7211.7* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.7211.7* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.7211.7* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.7212.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 3.212.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 3.212.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.212.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.212.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 40.37 red. biolum.^ Toxic 1.9412.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.9412.5* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.9412.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 1.9413.7* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.3713.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.57 EC50 biolum.# Not toxic 2.3713.7* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 77.5 % survival Not toxic 2.3713.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.22 red. biolum.^ Not toxic 2.3713.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.0213.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.0213.8* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 red. biolum.^ Not toxic 4.0213.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02

G-33


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

13.8* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.0214.0* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.614.0* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.614.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 17.4914.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.4914.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.4914.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.4914.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 50 % survival Not toxic 3.214.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.1 EC50 biolum.# Not toxic 3.214.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 45.6 red. biolum.^ Toxic 3.215.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.82 red. biolum.^ Not toxic 2.3415.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.3415.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.28 EC50 biolum.# Toxic 2.3415.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.3415.8* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 2.915.8* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.916.6* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 2.3416.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.3416.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.3416.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.3416.6* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.3416.8* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.73516.8* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.73516.8* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.73516.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.73516.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.73516.9* Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.8116.9* Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.8116.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 red. biolum.^ Not toxic 13.8116.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81

G-34


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

17.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 55 % survival Not toxic 3.3217.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 3.3217.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 3.3217.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.1 red. biolum.^ Not toxic 3.3218.0 Duluth-Superior Harbor-Allouez Bay 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR18.0 Duluth-Superior Harbor-Allouez Bay 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR19.0* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 2.919.0* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.919.7* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.319.7* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 2.320.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.720.0 Superior Bay 48-h Cladoceran Ceriodaphnia dubia Adult 100 % survival Not toxic 6.720.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.720.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.720.2* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 2.520.2* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Toxic 2.520.6* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 red. biolum.^ Not toxic 4.8420.6* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.8420.6* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.8420.6* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.8421.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.421.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.421.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.421.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 red. biolum.^ Not toxic 3.421.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.421.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.421.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 red. biolum.^ Not toxic 3.421.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.421.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.421.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4

G-35


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

22.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 2.9322.2* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.9322.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 13.8 red. biolum.^ Not toxic 2.9322.4* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.0222.4* St. Louis Bay 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.0222.4* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 4.0222.4* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 4.0223.0 Duluth-Superior Harbor-North of Minnesota 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR23.0 Duluth-Superior Harbor-North of Minnesota 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR

23.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 3.0723.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.0723.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.0723.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 3.0723.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.45 EC50 biolum.# Toxic 2.8823.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.8823.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.8823.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 31.2 red. biolum.^ Toxic 2.6723.5* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.6723.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 91 % survival Not toxic 2.6723.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 2.6723.6 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.423.6 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.423.6 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.423.6 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.424.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 35 % survival Not toxic 3.3824.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.3824.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 3.3824.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 19.2 red. biolum.^ Not toxic 3.3825.0* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.3625.0* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36

G-36


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

25.0* St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.3625.0* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 red. biolum.^ Not toxic 4.3625.0 Duluth-Superior Harbor-Hallett Coal Dock #6 10 day Diptera Chironomus tentans Unknown 86.6 % survival Not toxic NR25.0 Duluth-Superior Harbor-Hallett Coal Dock #6 48-h Cladoceran Daphnia magna Unknown 95 % survival Not toxic NR25.0* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 red. biolum.^ Toxic 13.9125.0* St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.9125.0* St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.9125.0* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.9125.0* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.9126.4* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1226.4* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1226.5* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.6926.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 1 red. biolum.^ Not toxic 3.6926.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.6926.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.6927.0* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 10.6827.0* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 10.6827.0* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 14.8 red. biolum.^ Not toxic 10.6827.0* Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.9527.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 red. biolum.^ Toxic 3.9527.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.9527.0* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.9527.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.9527.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 red. biolum.^ Toxic 3.9528.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 red. biolum.^ Not toxic 5.3428.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.3428.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.3428.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.3430.0* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 530.0* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5

G-37


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

31.6* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.9831.6* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.9831.6* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 red. biolum.^ Toxic 13.9831.6* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.9832.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.9732.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.9732.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 8.9732.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.9732.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.9733.0 Duluth-Superior Harbor-Northwest side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.433.0 Duluth-Superior Harbor-Northwest side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.433.0 Duluth-Superior Harbor-Northwest side of Lakehead Dock 48-h Cladoceran Ceriodaphnia dubia Adult 100 % survival Not toxic 8.433.0 Duluth-Superior Harbor-Northwest side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.433.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.4133.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.3 EC50 biolum.# Toxic 4.4133.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.4133.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 4.4133.8* Spirit Lake 10 day Amphipod Hyalella azteca JUV 41 % survival Toxic 4.2433.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.12 EC50 biolum.# Toxic 4.2433.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.2433.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 53.2 red. biolum.^ Toxic 4.2434.7* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.8734.7* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.8734.7* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.8734.7* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic 3.8734.7* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.8735.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.1335.2* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.1335.2* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.1335.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 red. biolum.^ Toxic 4.13

G-38


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

36.5* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.4836.5* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.4836.5* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 red. biolum.^ Toxic 4.4837.7 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.0837.7 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.0837.7 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.0837.7 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.0839.2* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.8439.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.8439.2* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.8439.2* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 29.8439.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.8439.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.7739.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 red. biolum.^ Toxic 6.7739.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.7739.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.7739.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.7740.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 red. biolum.^ Not toxic 3.9840.9* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.9840.9* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.9840.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.9843.6* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.0543.6* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.0543.6* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 red. biolum.^ Not toxic 4.0543.6* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.0544.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 4.1144.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.1144.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.1144.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.1144.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11

G-39


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

44.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.1144.1* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.1344.1* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.1344.1* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 red. biolum.^ Toxic 2.1344.1* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.1344.5* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.9944.5* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.9944.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.9944.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.5444.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 red. biolum.^ Toxic 4.5444.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.5444.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.5447.0 Duluth-Superior Harbor-Northeast of Western Lake Superior 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR47.0 Duluth-Superior Harbor-Northeast of Western Lake Superior 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR

49.3* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 6.549.3* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 6.549.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 3.9949.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 66.5 red. biolum.^ Toxic 3.9949.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 3.9949.5* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 10 % survival Toxic 3.9950.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 3.7650.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.25 EC50 biolum.# Toxic 3.7650.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 11.1 red. biolum.^ Not toxic 3.7650.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 44 % survival Not toxic 3.7651.1* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.651.1* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic 6.651.7* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.6351.7* St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.6351.7* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 red. biolum.^ Toxic 2.6351.7* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63

G-40


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

56.2 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.4256.2 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.4256.2 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.4256.2 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.4257.7* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.1957.7* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.1957.7* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 red. biolum.^ Toxic 14.1957.7* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.1957.8* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.9757.8* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.9757.8* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.9757.8* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.9757.8* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.9766.6 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.1266.9* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 red. biolum.^ Toxic 4.0566.9* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.0566.9* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.0566.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.0566.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.0571.3 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.5872.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.4772.3* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 red. biolum.^ Toxic 3.4772.3* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.4772.3* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.4777.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.5977.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.5977.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 red. biolum.^ Not toxic 5.5977.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.5981.2 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.8882.1* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 red. biolum.^ Not toxic 2.43

G-41


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

82.1* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.4382.1* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.4386.8* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.97586.8* Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.97586.8* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 red. biolum.^ Toxic 11.97586.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.97586.8* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.97594.5 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 1.894.5 Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.896.0 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.1696.0 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.1696.0 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.1696.0 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16101 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15101 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15101 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15101 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15104 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 3.9104 Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 3.9113 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 2.8130* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03130* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 red. biolum.^ Toxic 6.03130* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03131* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82131* Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82131* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 red. biolum.^ Toxic 0.82131* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82132 Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.2132 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic 2.2136* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 red. biolum.^ Not toxic 4.7

G-42


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

136* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7136* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7136* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7144 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1144 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1144 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1144 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1163 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic 3176 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76176 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76176 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76176 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76194* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1194* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1194* Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1194* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 red. biolum.^ Toxic 1194* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1194 Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 5.2194 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 5.2215 Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3215 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 3234 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR234 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR234 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR234 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR239 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95239 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95239 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95239 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95269 Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.2

G-43


Lead (mg/kg) Area

Test Duration


Life Stage


TOC (%)

269 Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.2308 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54308 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54308 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54308 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54370 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37370 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37370 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37370 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37389 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic NR389 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR389 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic NR389 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic NR668 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12668 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12668 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12668 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12

LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported. * = SEM measurement; ^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; red. Biolum. = reduced bioluminescence; EC50 = effective concentration affecting 50% of the population.

G-44

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-45

Reference

MPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-46

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997b

G-47

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-48

Reference

MPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-49

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-50

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997b

G-51

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-52

Reference

MPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-53

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bSmith et al. 1992Smith et al. 1992Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-54

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bSmith et al. 1992Smith et al. 1992

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-55

Reference

MPCA 1997bMPCA 1997bSmith et al. 1992Smith et al. 1992MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997

G-56

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-57

Reference

MPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-58

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bSmith et al. 1992Smith et al. 1992Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-59

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1996MPCA 1997c

G-60

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997b

G-61

Reference

MPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997

G-62

Reference

Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-63

Table G-5. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Copper in the St. Louis River Area of Concern

Copper (mg/kg) Area Test Duration Test Organism Species

Life Stage End-Point Measured Result

TOC (%)

0.578* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.20.578* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 red. biolum.^ Toxic 0.20.578* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.20.578* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.20.592* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.770.592* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.770.592* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 red. biolum.^ Toxic 6.770.592* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.770.592* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.770.715* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.080.715* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.080.715* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 red. biolum.^ Toxic 0.080.715* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.080.873* Spirit Lake 10 day Amphipod Hyalella azteca JUV 3 % survival Toxic 8.40.873* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 8.40.873* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.40.873* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 81.5 red. biolum.^ Toxic 8.40.906* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.140.906* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 red. biolum.^ Toxic 0.140.906* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.140.906* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.140.981* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.260.981* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.260.981* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 red. biolum.^ Toxic 8.260.981* Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.261.50* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 58 % survival Toxic 0.281.50* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.281.70* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 red. biolum.^ Toxic 0.161.70* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.161.70* Duluth-Superior Harbor 30 minute Bacteria Chironomus tentans NA 100 EC50 biolum.# Not toxic 0.161.98* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 1.55

G-45




TOC (%)

1.98* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.7 red. biolum.^ Not toxic 1.551.98* St. Louis Bay 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 1.552.01* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.122.01* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.122.01* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 red. biolum.^ Not toxic 0.122.01* St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.122.01* Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.352.01* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.352.01* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 red. biolum.^ Toxic 0.352.01* Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.652.01* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.652.01* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.652.01* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.652.01* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.652.04* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.42.04* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 red. biolum.^ Toxic 0.42.04* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.42.04* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.42.04* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.42.04* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.42.15* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.592.15* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 0.592.15* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.592.49* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.482.49* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.482.49* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 red. biolum.^ Toxic 4.482.51* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 9.08 EC50 biolum.# Not toxic 0.142.51* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 64 % survival Not toxic 0.142.51* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 0.142.51* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 45.3 red. biolum.^ Toxic 0.142.59* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71

G-46




TOC (%)

2.59* Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.712.59* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 1.712.59* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.712.59* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.712.64* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.242.64* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.242.64* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.242.64* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 red. biolum.^ Toxic 0.242.90* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 1.692.90* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.692.90* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.693.08* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 red. biolum.^ Not toxic 1.443.08* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.443.08* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.443.27* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.373.27* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.373.27* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.373.27* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.373.27* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 red. biolum.^ Not toxic 1.373.45* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.473.45* Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.473.45* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 red. biolum.^ Toxic 4.473.45* Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.473.70* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 0.863.70* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 0.863.70* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 14.18 EC50 biolum.# Not toxic 0.863.70* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 0.863.76* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 5.36 EC50 biolum.# Not toxic 0.613.76* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 0.613.76* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 0.613.76* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.61

G-47




TOC (%)

3.90* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 6.723.90* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.723.90* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.723.90* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.723.90* St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.723.93* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 0.893.93* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 0.893.94* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 21.31 red. biolum.^ Toxic 0.683.94* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.683.94* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.83 EC50 biolum.# Not toxic 0.684.31* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.024.31* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.024.31* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.024.31* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 red. biolum.^ Not toxic 4.024.31* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.024.44* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.974.44* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.974.44* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.974.44* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.974.44* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 8.974.54* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.74.54* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.74.54* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 2.74.54* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.74.54* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.74.54* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.74.54* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 red. biolum.^ Not toxic 2.74.54* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.74.54* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.74.54* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.74.70* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91

G-48




TOC (%)

4.70* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 red. biolum.^ Toxic 13.914.70* St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.914.70* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.914.70* St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.915.21* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.985.21* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.985.21* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.985.21* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.985.21* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 red. biolum.^ Not toxic 2.985.53* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.565.53* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 0.565.53* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.12 EC50 biolum.# Not toxic 0.565.53* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 0.565.57* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 red. biolum.^ Toxic 3.075.57* Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.075.57* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.075.61* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.825.61* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.825.61* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 red. biolum.^ Not toxic 1.825.61* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.825.78* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 3.25.78* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 3.25.78* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25.78* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.25.94* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.495.94* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.495.94* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.495.94* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 17.496.06* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.9 red. biolum.^ Toxic 0.816.06* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 0.816.06* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.06 EC50 biolum.# Not toxic 0.81

G-49




TOC (%)

6.06* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic 0.816.22* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.75 EC50 biolum.# Not toxic 1.856.22* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 1.856.22* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 14.9 red. biolum.^ Not toxic 1.856.22* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 1.857.01* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 27.4 red. biolum.^ Toxic 2.197.01* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.197.01* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.197.01* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 4.18 EC50 biolum.# Not toxic 2.197.22* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 red. biolum.^ Toxic 3.257.22* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.257.22* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.257.42* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.46 EC50 biolum.# Not toxic 2.47.42* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.47.42* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.47.42* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 35.18 red. biolum.^ Toxic 2.47.45 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.647.45 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.647.45 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.647.45 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.647.52* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.027.52* Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.027.52* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 red. biolum.^ Toxic 1.027.52* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.027.90* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.4 EC50 biolum.# Not toxic NR7.90* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic NR7.90* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 12 % survival Toxic NR7.90* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 60 % survival Not toxic NR7.97* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.857.97* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.857.97* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85

G-50




TOC (%)

7.97* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.857.97* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.858.18* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 1.758.18* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.758.18* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 1.758.18* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.87 red. biolum.^ Toxic 1.758.39* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.248.39* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.248.39* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.248.39* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.248.39* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.248.49* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 7.18.49* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 7.18.67* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 2.38.67* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.38.86* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.198.86* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.198.86* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 red. biolum.^ Toxic 14.198.86* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.199.19* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.7359.19* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.7359.19* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.7359.19* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.7359.19* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.7359.23* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.029.23* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.029.23* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 3.029.23* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.029.42* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.779.42* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.779.42* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77

G-51




TOC (%)

9.42* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 red. biolum.^ Not toxic 5.779.48* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.9759.48* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 red. biolum.^ Toxic 11.9759.48* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.9759.48* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.9759.48* Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.9759.84* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.4 red. biolum.^ Toxic 3.149.84* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.149.84* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.1410.2* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.3610.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 red. biolum.^ Not toxic 4.3610.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.3610.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.3610.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.410.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.410.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.410.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 red. biolum.^ Not toxic 3.410.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.410.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.410.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.410.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.410.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 red. biolum.^ Not toxic 3.410.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.410.7* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.3710.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.64 EC50 biolum.# Not toxic 2.3710.7* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 2.3710.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 28.9 red. biolum.^ Toxic 2.3710.8* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.910.8* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 2.910.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 1.110.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.1

G-52




TOC (%)

10.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.110.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.110.9* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.210.9* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.31 red. biolum.^ Not toxic 2.210.9* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 2.210.9* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.211.9* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 red. biolum.^ Toxic 1.8711.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.8711.9* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.8711.9* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.8711.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.8712.0* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic 3.8712.0* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.8712.0* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.8712.0* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.8712.0* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.8712.0* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.8412.0* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.8412.0* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.8412.0* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 red. biolum.^ Not toxic 4.8412.3* Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.8112.3* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 red. biolum.^ Not toxic 13.8112.3* Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.8112.3* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.8112.4* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 3.17 red. biolum.^ Not toxic 2.8212.4* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.23 EC50 biolum.# Toxic 2.8212.4* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.8212.4* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 2.8212.4* Spirit Lake 10 day Diptera Chironomus tentans LAR 50 % survival Not toxic 3.212.4* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.1 EC50 biolum.# Not toxic 3.212.4* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 45.6 red. biolum.^ Toxic 3.2

G-53




TOC (%)

12.6* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.9112.6* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 red. biolum.^ Not toxic 2.9112.6* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.9112.6* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.9112.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 10.6812.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 10.6812.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 14.8 red. biolum.^ Not toxic 10.6812.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 1 red. biolum.^ Not toxic 3.6912.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.6912.9* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.6912.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.6913.1* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 2.513.1* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Toxic 2.513.3* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.3413.3* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.3413.3* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.3413.3* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 red. biolum.^ Not toxic 5.3413.3* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 1.813.3* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.813.4* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 3.3213.4* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.1 red. biolum.^ Not toxic 3.3213.4* Spirit Lake 10 day Amphipod Hyalella azteca JUV 55 % survival Not toxic 3.3213.4* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 3.3213.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 1.9413.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.9413.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.9413.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 40.37 red. biolum.^ Toxic 1.9413.7* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1213.7* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1214.4* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 red. biolum.^ Not toxic 2.4314.4* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43

G-54




TOC (%)

14.4* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.4314.4 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.4214.4 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.4214.4 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.4214.4 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.4214.5* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.9914.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.9914.5* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.9915.0* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.615.0* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.615.3* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 2.915.3* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.915.4* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 515.4* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 515.5* Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.9515.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.9515.5* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.9515.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.9515.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 red. biolum.^ Toxic 3.9515.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 red. biolum.^ Toxic 3.9515.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 2.3415.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.3415.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.3415.5* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.3415.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.3415.8* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.8815.8* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.45 EC50 biolum.# Toxic 2.8815.8* St. Louis Bay 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.8815.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.3515.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.3515.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.35

G-55




TOC (%)

15.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.3516.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.4216.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.4216.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 red. biolum.^ Toxic 1.4216.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.4216.0* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 77.5 % survival Not toxic 2.3716.0* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.22 red. biolum.^ Not toxic 2.3716.0* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.3716.0* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.57 EC50 biolum.# Not toxic 2.3716.2* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.1316.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 red. biolum.^ Toxic 4.1316.2* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.1316.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.1316.3* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.3416.3* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.3416.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.28 EC50 biolum.# Toxic 2.3416.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.82 red. biolum.^ Not toxic 2.3416.7* St. Louis Bay-St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.8416.7* St. Louis Bay-St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 29.8416.7* St. Louis Bay-St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.8416.7* St. Louis Bay-St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.8416.7* St. Louis Bay-St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.8416.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.0516.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.0516.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 red. biolum.^ Not toxic 4.0516.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.0517.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 2.6717.2* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.6717.2* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 91 % survival Not toxic 2.6717.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 31.2 red. biolum.^ Toxic 2.6717.4* St. Louis Bay 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.02

G-56




TOC (%)

17.4* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 4.0217.4* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 4.0217.4* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.0217.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.5917.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.5917.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.5917.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 2.5918.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.3 EC50 biolum.# Toxic 4.4118.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.4118.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.4118.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 4.4118.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.9818.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.9818.8* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.9818.8* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 red. biolum.^ Not toxic 3.9818.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 2.9318.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 13.8 red. biolum.^ Not toxic 2.9318.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.9319.0* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 6.519.0* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 6.519.9* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.9819.9* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.9819.9* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.9819.9* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 red. biolum.^ Toxic 13.9820.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.0720.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.0720.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 3.0720.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 3.0720.4 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.9520.4 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.9520.4 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95

G-57




TOC (%)

20.4 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.9520.9* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.620.9* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic 6.620.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 red. biolum.^ Not toxic 2.8220.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.8220.9* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.8220.9* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.8221.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 4.1121.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.1121.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.1121.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.1121.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.1121.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.1121.6* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.1321.6* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.1321.6* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 red. biolum.^ Toxic 2.1321.6* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.1322.6* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 3.3822.6* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 19.2 red. biolum.^ Not toxic 3.3822.6* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.3822.6* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 35 % survival Not toxic 3.3823.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 3.7623.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 11.1 red. biolum.^ Not toxic 3.7623.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 44 % survival Not toxic 3.7623.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.25 EC50 biolum.# Toxic 3.7624.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.5924.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.5924.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 red. biolum.^ Not toxic 5.5924.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.5924.2* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.0524.2* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05

G-58




TOC (%)

24.2* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.0524.2* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.0524.2* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 red. biolum.^ Toxic 4.0524.5* Spirit Lake 10 day Amphipod Hyalella azteca JUV 41 % survival Toxic 4.2424.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.2424.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.12 EC50 biolum.# Toxic 4.2424.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 53.2 red. biolum.^ Toxic 4.2425.1 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.0825.1 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.0825.1 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.0825.1 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.0825.3 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.425.3 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.425.3 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.425.3 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.425.5* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.9725.5* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.9725.5* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.9725.5* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.9725.5* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.9725.6* St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.6325.6* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 red. biolum.^ Toxic 2.6325.6* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.6325.6* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.6326.7* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 3.926.7* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 3.926.9 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.9326.9 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.9326.9 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.9326.9 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.9329.1* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.2

G-59




TOC (%)

29.1* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic 2.229.4* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 5.229.4* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 5.232.6* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 10 % survival Toxic 3.9932.6* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 3.9932.6* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 3.9932.6* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 66.5 red. biolum.^ Toxic 3.9939.3* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic 340.1* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 340.1* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 340.2* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.4740.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 red. biolum.^ Toxic 3.4740.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.4740.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.4742.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.5442.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 red. biolum.^ Toxic 4.5442.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.5442.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.5453.2 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.1254.6* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.0354.6* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 red. biolum.^ Toxic 6.0354.6* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.0361.2 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.5861.9 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.1661.9 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.1661.9 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.1661.9 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.1663.2* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.763.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.763.2* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.763.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 red. biolum.^ Not toxic 4.7

G-60




TOC (%)

64.6 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.8865.3* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.265.3* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.272.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.1572.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.1572.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.1572.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.1573.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 red. biolum.^ Toxic 0.8273.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.8273.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.8273.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.8278.0* Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 178.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 178.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 red. biolum.^ Toxic 178.0* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 178.0* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1101 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic NR101 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic NR101 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR101 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic NR121 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1121 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1121 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1121 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1122 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12122 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12122 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12122 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12187 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR187 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR187 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR

G-61




TOC (%)

187 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR194* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 2.8199 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76199 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76199 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76199 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76248 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54248 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54248 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54248 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54291 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37291 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37291 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37291 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37

* = SEM measurement; ^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; red. Biolum. = reduced bioluminescence; EC50 = effective concentration affecting 50% of the population.LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-62

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-63

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997c

G-64

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-65

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-66

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-67

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997c

G-68

Reference

MPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-69

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997b

G-70

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-71

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997c

G-72

Reference

MPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-73

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-74

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996

G-75

Reference

IT Corp. 1996Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997c

G-76

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997

G-77

Reference

Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-78

Reference

MPCA 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-79

Reference

IT Corp. 1996Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-80

Table G-6. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Mercury in the St. Louis River Area of Concern

Mercury(mg/kg) Area Test Duration


TOC (%)

<0.004 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35<0.004 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.35<0.004 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 mg growth Not toxic 0.350.005 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.930.005 St. Louis River - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.930.005 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.930.005 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93

<0.005 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12<0.005 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12<0.005 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12<0.005 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12<0.006 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16<0.006 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16<0.006 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16<0.008 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59<0.008 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59<0.008 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.59<0.009 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.83 EC50 biolum.# Not toxic 0.68<0.009 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.68<0.009 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 21.31 % red. biolum.^ Toxic 0.68<0.010 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08<0.010 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08<0.010 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08<0.010 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08<0.013 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24<0.013 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24<0.013 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24<0.013 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24<0.014 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2<0.014 D-S Harbor 10 day Amphipod Chironomus tentans JUV 86 % survival Not toxic 0.2<0.014 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2

G-63




TOC (%)

<0.014 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.2<0.015 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4<0.015 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4<0.015 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4<0.015 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4<0.015 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4<0.015 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4<0.017 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 0.56<0.017 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.12 EC50 biolum.# Not toxic 0.56<0.017 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.56<0.017 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 0.56<0.02 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 9.08 EC50 biolum.# Not toxic 0.14<0.02 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7<0.02 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7<0.02 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7<0.02 D-S Harbor 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 0.14<0.02 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 45.3 % red. biolum.^ Toxic 0.14<0.02 D-S Harbor 10 day Amphipod Hyalella azteca JUV 64 % survival Not toxic 0.14<0.02 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7

<0.023 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14<0.023 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14<0.023 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14<0.023 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.140.025 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.10.025 D-S Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.10.025 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 1.10.025 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.10.026 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.020.026 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.020.026 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.020.026 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02

G-64




TOC (%)

0.026 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 14.18 EC50 biolum.# Not toxic 0.860.026 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 0.860.026 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.8 % red. biolum.^ Not toxic 0.860.026 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 0.860.028 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.420.028 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.420.028 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.420.028 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.420.028 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.690.028 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.690.028 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.690.030 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.640.030 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR0.030 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.640.030 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR0.030 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.640.030 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.640.032 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.870.032 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.870.032 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.870.032 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.870.032 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.870.034 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 5.36 EC50 biolum.# Not toxic 0.610.034 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 0.610.034 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.610.034 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 0.610.034 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.770.034 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.770.034 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.770.034 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.770.040 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42

G-65




TOC (%)

0.040 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.420.040 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.420.040 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.420.041 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 4.18 EC50 biolum.# Not toxic 2.190.041 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.190.041 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 27.4 % red. biolum.^ Toxic 2.190.041 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.190.044 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR0.044 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR0.049 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.820.049 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.820.049 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.820.049 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.820.050 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.46 EC50 biolum.# Not toxic 2.40.050 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.40.050 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 35.18 % red. biolum.^ Toxic 2.40.050 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.40.050 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.20.050 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 2.20.050 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.31 % red. biolum.^ Not toxic 2.20.050 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.20.051 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.650.051 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.650.051 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.650.051 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.650.051 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.650.052 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.350.052 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.350.052 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.350.052 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.350.052 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07

G-66




TOC (%)

0.052 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.070.052 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.070.055 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.710.055 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.710.055 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.710.055 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.710.055 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.710.056 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.06 EC50 biolum.# Not toxic 0.810.056 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 0.810.056 D-S Harbor 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic 0.810.056 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.9 % red. biolum.^ Toxic 0.810.057 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.020.057 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.020.057 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 3.020.057 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.020.058 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.470.058 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.470.058 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.470.058 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.470.059 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.23 EC50 biolum.# Toxic 2.820.059 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 2.820.059 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 3.17 % red. biolum.^ Not toxic 2.820.059 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.820.064 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.370.064 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.370.064 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.370.064 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.370.064 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.370.066 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 2.370.066 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.370.066 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 28.9 % red. biolum.^ Toxic 2.37

G-67




TOC (%)

0.066 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.64 EC50 biolum.# Not toxic 2.370.069 D-S Harbor 10 day Diptera Chironomus tentans LAR 10 % survival Toxic 3.990.069 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 66.5 % red. biolum.^ Toxic 3.990.069 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 3.990.069 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 3.990.069 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 60 % survival Not toxic NR0.069 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.4 EC50 biolum.# Not toxic NR0.069 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic NR0.069 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 12 % survival Toxic NR0.071 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.440.071 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.440.071 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.440.072 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 10.072 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 10.072 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 10.072 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 10.072 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 10.072 D-S Harbor 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 1.850.072 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.75 EC50 biolum.# Not toxic 1.850.072 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 1.850.072 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 14.9 % red. biolum.^ Not toxic 1.850.076 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.820.076 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.820.076 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.820.076 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.820.076 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.980.076 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.980.076 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.980.076 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.980.076 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.980.077 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24

G-68




TOC (%)

0.077 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.240.077 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.240.077 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.240.077 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.240.078 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.910.078 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.910.078 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.910.078 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.910.078 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.590.078 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.590.078 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.590.078 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.590.080 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 1.750.080 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.750.080 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.87 % red. biolum.^ Toxic 1.750.080 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 1.750.088 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.70.088 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.70.088 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.70.088 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.70.088 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.70.088 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.70.088 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.70.088 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.70.088 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.70.088 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.70.089 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.850.089 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.850.089 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.850.089 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.850.089 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85

G-69




TOC (%)

0.089 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.7 % red. biolum.^ Not toxic 1.550.089 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 1.550.089 St. Louis Bay 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 1.550.093 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.340.093 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.340.093 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.340.093 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.340.093 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.340.094 Spirit Lake 10 day Amphipod Hyalella azteca JUV 3 % survival Toxic 8.40.094 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 8.40.094 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.40.094 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 81.5 % red. biolum.^ Toxic 8.40.096 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.140.096 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.4 % red. biolum.^ Toxic 3.140.096 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.140.100 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.840.100 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.840.100 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.840.100 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.840.101 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.810.101 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.810.101 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.810.101 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.810.104 D-S Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.940.104 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 40.37 % red. biolum.^ Toxic 1.940.104 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.940.104 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 1.940.121 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.340.121 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.28 EC50 biolum.# Toxic 2.340.121 D-S Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.340.121 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.82 % red. biolum.^ Not toxic 2.34

G-70




TOC (%)

0.122 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.820.122 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.820.122 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.820.122 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.820.128 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.20.128 D-S Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 3.20.128 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 3.20.128 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.20.130 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.250.130 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.250.130 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.250.137 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 45.6 % red. biolum.^ Toxic 3.20.137 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.1 EC50 biolum.# Not toxic 3.20.137 Spirit Lake 10 day Diptera Chironomus tentans LAR 50 % survival Not toxic 3.20.150 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.40.150 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.40.150 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.40.150 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.40.159 St. Louis Bay - St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.630.159 St. Louis Bay - St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.630.159 St. Louis Bay - St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.630.159 St. Louis Bay - St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.630.160 D-S Harbor - North of MN Power & Light Dock 10 day Diptera Chironomus tentans ? 100 % survival Not toxic NR0.160 D-S Harbor - North of MN Power & Light Dock 48 hour Cladoceran Daphnia magna ? 100 % survival Not toxic NR0.160 Brule River - above Stones Bridge 48 hour Cladoceran Daphnia magna ? 95 % survival Not toxic NR0.160 Brule River - above Stones Bridge 10 day Diptera Chironomus tentans ? 100 % survival Not toxic NR0.161 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.910.161 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.910.161 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.910.161 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.910.161 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91

G-71




TOC (%)

0.168 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.740.168 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.740.168 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.740.168 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.740.168 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.740.180 D-S Harbor - Hallett Coal Dock #6 48 hour Cladoceran Daphnia magna ? 95 % survival Not toxic NR0.180 D-S Harbor - Hallett Coal Dock #6 10 day Diptera Chironomus tentans ? 86.6 % survival Not toxic NR0.182 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.720.182 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.720.182 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.720.182 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.720.182 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.720.184 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 3.070.184 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 3.070.184 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.070.184 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.070.188 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.45 EC50 biolum.# Toxic 2.880.188 St. Louis Bay 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.880.188 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.880.189 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.490.189 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.490.189 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.490.189 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.490.190 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.40.190 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.40.190 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.40.190 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.40.194 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 3.320.194 Spirit Lake 10 day Amphipod Hyalella azteca JUV 55 % survival Not toxic 3.320.194 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.1 % red. biolum.^ Not toxic 3.320.194 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 3.32

G-72




TOC (%)

0.200 D-S Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.930.200 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 13.8 % red. biolum.^ Not toxic 2.930.200 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 2.930.209 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 3.380.209 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 19.2 % red. biolum.^ Not toxic 3.380.209 D-S Harbor 10 day Diptera Chironomus tentans LAR 35 % survival Not toxic 3.380.209 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.380.210 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.950.210 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.950.210 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.950.210 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.950.210 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.950.210 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.950.210 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic NR0.210 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR0.211 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.40.211 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.40.211 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.40.211 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.40.211 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.40.211 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.40.211 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.40.211 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.40.211 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.40.211 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.40.215 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 4.020.215 St. Louis Bay 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.020.215 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.020.215 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 4.020.220 D-S Harbor - City ofSuperior WWTP 10 day Amphipod Hyalella azteca JUV 79 % survival Not toxic NR0.220 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR

G-73




TOC (%)

0.221 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.260.221 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.260.221 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.260.221 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.260.224 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 14.8 % red. biolum.^ Not toxic 10.680.224 Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 10.680.224 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 10.680.229 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.360.229 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.360.229 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.360.229 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.360.230 D-S Harbor - City ofSuperior WWTP 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR0.233 D-S Harbor 10 day Amphipod Hyalella azteca JUV 91 % survival Not toxic 2.670.233 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 2.670.233 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 31.2 % red. biolum.^ Toxic 2.670.233 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.670.240 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR0.240 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR0.240 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR0.240 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR0.250 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.10.250 D-S Harbor - Allouez Bay 10 day Diptera Chironomus tentans ? 100 % survival Not toxic NR0.250 D-S Harbor - Allouez Bay 48 hour Cladoceran Daphnia magna ? 100 % survival Not toxic NR0.250 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.10.250 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.10.250 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.10.252 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.22 % red. biolum.^ Not toxic 2.370.252 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.57 EC50 biolum.# Not toxic 2.370.252 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.370.252 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 77.5 % survival Not toxic 2.370.260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR

G-74




TOC (%)

0.260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR0.260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR0.260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR0.262 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 1 % red. biolum.^ Not toxic 3.690.262 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.690.262 D-S Harbor 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.690.262 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.690.264 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.970.264 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.970.264 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.970.264 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.970.264 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.970.269 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.590.269 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.590.269 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.590.269 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.590.269 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.050.269 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.050.269 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.050.269 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.050.269 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.050.280 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR0.280 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR0.288 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.980.288 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.980.288 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.980.288 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.980.291 Spirit Lake 10 day Amphipod Hyalella azteca JUV 41 % survival Toxic 4.240.291 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 53.2 % red. biolum.^ Toxic 4.240.291 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.12 EC50 biolum.# Toxic 4.240.291 Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.24

G-75




TOC (%)

0.309 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.430.309 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.430.309 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.430.310 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.080.310 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic NR0.310 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR0.310 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.080.310 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.080.310 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.080.312 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.020.312 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.020.312 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.020.312 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.020.312 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.020.319 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.880.336 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.130.336 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.130.336 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.130.336 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.130.348 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.980.348 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.980.348 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.980.348 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.980.348 Spirit Lake 10 day Diptera Chironomus tentans LAR -9 mg growth Not determined 11.980.350 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR0.350 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR0.360 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.760.360 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR0.360 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR0.360 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.760.360 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR

G-76




TOC (%)

0.360 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.760.360 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.760.364 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.980.364 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.980.364 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.980.364 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.980.370 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.950.370 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.950.370 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.950.370 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.950.389 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.470.389 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.470.389 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.470.389 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.470.398 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.340.398 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.340.398 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.340.398 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.340.413 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.120.430 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.840.430 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.840.430 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.840.430 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.840.430 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.840.432 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.030.432 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.030.432 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.030.433 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.870.433 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.870.433 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.870.433 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87

G-77




TOC (%)

0.433 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.870.440 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.70.440 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.70.440 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.70.440 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.70.440 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.150.440 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.150.440 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.150.440 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.150.460 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR0.460 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR0.463 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.540.463 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.540.463 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.540.463 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.540.490 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR0.500 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR0.500 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR0.500 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR0.502 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.970.502 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.970.502 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.970.502 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.970.502 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.970.520 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR0.520 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR0.529 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.110.529 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.110.529 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.110.529 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.110.540 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR

G-78




TOC (%)

0.540 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR0.540 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic NR0.540 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR0.559 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.770.559 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.770.559 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.770.559 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.770.559 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.770.573 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR0.573 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic NR0.589 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.580.611 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.990.611 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.990.611 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.990.611 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 11.1 % red. biolum.^ Not toxic 3.760.611 D-S Harbor 10 day Amphipod Hyalella azteca JUV 44 % survival Not toxic 3.760.611 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.25 EC50 biolum.# Toxic 3.760.611 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 3.760.656 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.050.656 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.050.656 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.050.656 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.050.671 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.110.671 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.8 % red. biolum.^ Not toxic 4.410.671 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.410.671 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.110.671 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.410.671 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.3 EC50 biolum.# Toxic 4.410.700 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 1000.700 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 1000.700 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100

G-79




TOC (%)

0.700 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1000.702 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.130.702 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.130.702 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.130.702 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.130.710 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR0.710 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR0.720 D-S Harbor - Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR0.720 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic NR0.720 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR0.750 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR0.760 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.160.760 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.160.760 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.160.760 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.160.790 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR0.870 D-S Harbor - City ofSuperior WWTP 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic NR0.870 D-S Harbor - City ofSuperior WWTP 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR0.920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR0.966 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.190.966 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.190.966 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.190.966 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.190.980 D-S Harbor - Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR0.980 D-S Harbor - NE of Sanitary District Plant 10 day Diptera Chironomus tentans ? 100 % survival Not toxic NR0.980 D-S Harbor - NE of Sanitary District Plant 48 hour Cladoceran Daphnia magna ? 100 % survival Not toxic NR1.26 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.481.26 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.481.26 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.481.31 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR1.31 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR

G-80




TOC (%)

1.31 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR1.31 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR1.42 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.541.42 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.541.42 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.541.42 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.541.50 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR1.50 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR2.15 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.122.15 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.122.15 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.122.15 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.122.73 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.372.73 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.372.73 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.372.73 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37

* = SEM measurement; ^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; red. biolum. = reduced bioluminescence; EC50 = effective concentration affecting 50% of the population.LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-81

Reference

MPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-82

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bWenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-83

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996Crane et al. 1997IT Corp. 1996Crane et al. 1997IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996

G-84

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-85

Reference

MPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997b

G-86

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-87

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-88

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-89

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bSmith et al. 1992Smith et al. 1992Smith et al. 1992Smith et al. 1992MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-90

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cSmith et al. 1992Smith et al. 1992MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-91

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997

G-92

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997IT Corp. 1996Smith et al. 1992Smith et al. 1992IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997

G-93

Reference

Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-94

Reference

MPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997IT Corp. 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996Crane et al. 1997

G-95

Reference

IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1993MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-96

Reference

MPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997

G-97

Reference

Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1993MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996

G-98

Reference

IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Smith et al. 1992Smith et al. 1992MPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996

G-99

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-100

Table G-7. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Zinc in the St. Louis River Area of Concern

Zinc Test Test TOC(mg/kg) Area Duration Organism Species Life Stage End-Point Measured Result (%) Reference

4.35* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b4.35* Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b4.35* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 red. biolum.^ Toxic 0.35 MPCA 1997b5.24* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b5.24* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b5.24* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 0.59 MPCA 1997b6.19* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 red. biolum.^ Toxic 0.16 MPCA 1997b6.19* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b6.19* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b6.73* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 red. biolum.^ Toxic 0.08 MPCA 1997b6.73* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08 MPCA 1997b6.73* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b6.73* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b7.76* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 21.31 red. biolum.^ Toxic 0.68 MPCA 1997b7.76* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.83 EC50 biolum.# Not toxic 0.68 MPCA 1997b7.76* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.68 MPCA 1997b7.79* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b7.79* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b7.79* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b7.79* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 red. biolum.^ Toxic 0.4 MPCA 1997b7.79* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b7.79* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b7.90* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 red. biolum.^ Toxic 0.2 MPCA 1997b7.90* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b7.90* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b7.90* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b9.17* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 red. biolum.^ Toxic 0.14 MPCA 1997b9.17* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b9.17* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b9.17* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b10.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 red. biolum.^ Toxic 0.24 MPCA 1997b10.2* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b10.2* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b

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10.2* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b11.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 45.3 red. biolum.^ Toxic 0.14 MPCA 1997b11.1* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 9.08 EC50 biolum.# Not toxic 0.14 MPCA 1997b11.1* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 64 % survival Not toxic 0.14 MPCA 1997b11.1* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 0.14 MPCA 1997b11.2* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 58 % survival Toxic 0.28 Crane et al. 199711.2* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.28 Crane et al. 199713.5* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 red. biolum.^ Not toxic 1.37 MPCA 1997c13.5* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c13.5* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c13.5* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c13.5* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c14.8* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 0.89 Crane et al. 199714.8* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 0.89 Crane et al. 199715.0* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 red. biolum.^ Toxic 1.02 MPCA 1997b15.0* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b15.0* Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b15.0* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b16.6* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 14.18 EC50 biolum.# Not toxic 0.86 MPCA 1997b16.6* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 0.86 MPCA 1997b16.6* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 0.86 MPCA 1997b16.6* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 0.86 MPCA 1997b16.7* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 0.56 MPCA 1997b16.7* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 0.56 MPCA 1997b16.7* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.56 MPCA 1997b16.7* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.12 EC50 biolum.# Not toxic 0.56 MPCA 1997b16.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 red. biolum.^ Not toxic 1.44 MPCA 1997c16.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c16.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c17.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.1 MPCA 1997b17.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 1.1 MPCA 1997b17.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.1 MPCA 1997b17.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.1 MPCA 1997b

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18.3* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c18.3* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c18.3* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 red. biolum.^ Toxic 1.87 MPCA 1997c18.3* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c18.3* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c18.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 1.69 MPCA 1997c18.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c18.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c19.9* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b19.9* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 red. biolum.^ Not toxic 0.12 MPCA 1997b19.9* St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b19.9* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b20.0* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.61 MPCA 1997b20.0* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 5.36 EC50 biolum.# Not toxic 0.61 MPCA 1997b20.0* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 0.61 MPCA 1997b20.0* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 0.61 MPCA 1997b22.5* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b22.5* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 red. biolum.^ Toxic 4.47 MPCA 1997b22.5* Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b22.5* Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b25.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b25.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 red. biolum.^ Not toxic 5.77 MPCA 1997b25.2* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b25.2* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b26.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c26.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c26.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c26.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 red. biolum.^ Not toxic 2.98 MPCA 1997c26.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c27.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b27.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 red. biolum.^ Toxic 1.42 MPCA 1997b27.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b27.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b

G-84



28.4* St. Louis Bay 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 1.55 MPCA 1997b28.4* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 1.55 MPCA 1997b28.4* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.7 red. biolum.^ Not toxic 1.55 MPCA 1997b28.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic 0.81 MPCA 1997b28.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 0.81 MPCA 1997b28.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.9 red. biolum.^ Toxic 0.81 MPCA 1997b28.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.06 EC50 biolum.# Not toxic 0.81 MPCA 1997b28.5* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 7.1 Crane et al. 199728.5* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 7.1 Crane et al. 199729.3* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 red. biolum.^ Not toxic 1.82 MPCA 1997b29.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b29.3* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b29.3* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b29.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c29.5* Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c29.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.65 MPCA 1997c29.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c29.5* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c29.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c29.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c29.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c29.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c29.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.24 MPCA 1997c32.0* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c32.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 1.71 MPCA 1997c32.0* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c32.0* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c32.0* Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c32.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 1.85 MPCA 1997b32.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.75 EC50 biolum.# Not toxic 1.85 MPCA 1997b32.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 1.85 MPCA 1997b32.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 14.9 red. biolum.^ Not toxic 1.85 MPCA 1997b33.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c

G-85



33.7* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 red. biolum.^ Not toxic 4.02 MPCA 1997c33.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c33.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c33.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c34.3* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.4 EC50 biolum.# Not toxic NR MPCA 1997b34.3* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 12 % survival Toxic NR MPCA 1997b34.3* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic NR MPCA 1997b34.3* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 60 % survival Not toxic NR MPCA 1997b34.9* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 red. biolum.^ Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c34.9* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c37.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b37.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.35 MPCA 1997b37.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b37.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b40.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b40.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b40.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 red. biolum.^ Toxic 3.07 MPCA 1997b40.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 27.4 red. biolum.^ Toxic 2.19 MPCA 1997b40.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 4.18 EC50 biolum.# Not toxic 2.19 MPCA 1997b40.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.19 MPCA 1997b40.5* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.19 MPCA 1997b43.7* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.75 MPCA 1997b43.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.87 red. biolum.^ Toxic 1.75 MPCA 1997b43.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 1.75 MPCA 1997b43.7* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 1.75 MPCA 1997b

G-86



45.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c45.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c45.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c45.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c45.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.85 MPCA 1997c46.6* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.2 MPCA 1997b46.6* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.2 MPCA 1997b46.6* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 2.2 MPCA 1997b46.6* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.31 red. biolum.^ Not toxic 2.2 MPCA 1997b47.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.2 MPCA 1997b47.9* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.2 MPCA 1997b47.9* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 3.2 MPCA 1997b47.9* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 3.2 MPCA 1997b49.2* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.4 red. biolum.^ Toxic 3.14 MPCA 1997b49.2* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.14 MPCA 1997b49.2* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.14 MPCA 1997b50.0* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c50.0* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c50.0* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 red. biolum.^ Toxic 3.25 MPCA 1997c51.0* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 red. biolum.^ Not toxic 2.82 MPCA 1997b51.0* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b51.0* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b51.0* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b51.6* St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c51.6* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c51.6* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c51.6* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c51.6* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 6.72 MPCA 1997c52.1* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 2.59 MPCA 1997b52.1* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b52.1* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b52.1* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b53.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.94 MPCA 1997b

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53.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 40.37 red. biolum.^ Toxic 1.94 MPCA 1997b53.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.94 MPCA 1997b53.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 1.94 MPCA 1997b54.0* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.46 EC50 biolum.# Not toxic 2.4 MPCA 1997b54.0* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 35.18 red. biolum.^ Toxic 2.4 MPCA 1997b54.0* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.4 MPCA 1997b54.0* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.4 MPCA 1997b55.1* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 1.8 Crane et al. 199755.1* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.8 Crane et al. 199757.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c57.6* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 2.34 MPCA 1997c57.6* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c57.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c57.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c57.7 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 199657.7 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 199657.7 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 199657.7 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 199658.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 3.17 red. biolum.^ Not toxic 2.82 MPCA 1997b58.7* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 2.82 MPCA 1997b58.7* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.82 MPCA 1997b58.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.23 EC50 biolum.# Toxic 2.82 MPCA 1997b59.7* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b59.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b59.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 red. biolum.^ Not toxic 2.91 MPCA 1997b59.7* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b62.3* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 3.02 MPCA 1997b62.3* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.02 MPCA 1997b62.3* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.02 MPCA 1997b62.3* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.02 MPCA 1997b62.4* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.6 Crane et al. 199762.4* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.6 Crane et al. 199764.6* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 2.5 Crane et al. 1997

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64.6* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Toxic 2.5 Crane et al. 199766.0* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 red. biolum.^ Not toxic 4.84 MPCA 1997b66.0* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b66.0* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b66.0* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b66.4* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b66.4* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b66.4* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 17.49 MPCA 1997b66.4* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b68.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.82 red. biolum.^ Not toxic 2.34 MPCA 1997b68.3* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.34 MPCA 1997b68.3* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.34 MPCA 1997b68.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.28 EC50 biolum.# Toxic 2.34 MPCA 1997b68.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 3 % survival Toxic 8.4 MPCA 1997b68.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.4 MPCA 1997b68.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 8.4 MPCA 1997b68.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 81.5 red. biolum.^ Toxic 8.4 MPCA 1997b69.2* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 28.9 red. biolum.^ Toxic 2.37 MPCA 1997b69.2* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.37 MPCA 1997b69.2* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 2.37 MPCA 1997b69.2* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.64 EC50 biolum.# Not toxic 2.37 MPCA 1997b69.2* Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b69.2* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b69.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b69.2* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 red. biolum.^ Toxic 8.26 MPCA 1997b71.5* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 2.3 Crane et al. 199771.5* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.3 Crane et al. 199771.8* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.735 MPCA 1997c71.8* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.735 MPCA 1997c71.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.735 MPCA 1997c71.8* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.735 MPCA 1997c71.8* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.735 MPCA 1997c73.3* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.9 Crane et al. 1997

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73.3* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 2.9 Crane et al. 199773.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 red. biolum.^ Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 red. biolum.^ Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c73.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c73.7* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c73.7* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 red. biolum.^ Not toxic 2.43 MPCA 1997c73.7* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c76.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b76.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 red. biolum.^ Not toxic 13.81 MPCA 1997b76.9* Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b76.9* Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b77.5* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 2.9 Crane et al. 199777.5* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.9 Crane et al. 199777.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 50 % survival Not toxic 3.2 MPCA 1997b77.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.1 EC50 biolum.# Not toxic 3.2 MPCA 1997b77.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 45.6 red. biolum.^ Toxic 3.2 MPCA 1997b78.4* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5 Crane et al. 199778.4* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5 Crane et al. 199782.1* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 12 Crane et al. 199782.1* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 12 Crane et al. 199783.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 13.8 red. biolum.^ Not toxic 2.93 MPCA 1997b83.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.93 MPCA 1997b83.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 2.93 MPCA 1997b88.3* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 3.32 MPCA 1997b88.3* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.1 red. biolum.^ Not toxic 3.32 MPCA 1997b88.3* Spirit Lake 10 day Amphipod Hyalella azteca JUV 55 % survival Not toxic 3.32 MPCA 1997b

G-90



88.3* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 3.32 MPCA 1997b88.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c88.3* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c88.3* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 red. biolum.^ Toxic 13.91 MPCA 1997c88.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c88.3* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c91.3* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.69 MPCA 1997b91.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 1 red. biolum.^ Not toxic 3.69 MPCA 1997b91.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.69 MPCA 1997b91.3* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.69 MPCA 1997b91.3* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 3.07 MPCA 1997b91.3* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.07 MPCA 1997b91.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.07 MPCA 1997b91.3* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 3.07 MPCA 1997b93.5* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.67 MPCA 1997b93.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 2.67 MPCA 1997b93.5* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 91 % survival Not toxic 2.67 MPCA 1997b93.5* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 31.2 red. biolum.^ Toxic 2.67 MPCA 1997b95.5* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.45 EC50 biolum.# Toxic 2.88 MPCA 1997b95.5* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.88 MPCA 1997b95.5* St. Louis Bay 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.88 MPCA 1997b96.2* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b96.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b96.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b96.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 red. biolum.^ Toxic 2.63 MPCA 1997b99.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 red. biolum.^ Not toxic 4.36 MPCA 1997b99.2* St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b99.2* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b99.2* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b100 Duluth-Superior Harbor-Allouez Bay 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 1992100 Duluth-Superior Harbor-Allouez Bay 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR Smith et al. 1992103* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 red. biolum.^ Toxic 3.95 MPCA 1997c103* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 red. biolum.^ Toxic 3.95 MPCA 1997c

G-91



103* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c103* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c103* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c103* Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c106* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997b106* St. Louis Bay 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.02 MPCA 1997b106* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 4.02 MPCA 1997b106* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 4.02 MPCA 1997b107* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 3.38 MPCA 1997b107* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.38 MPCA 1997b107* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 35 % survival Not toxic 3.38 MPCA 1997b107* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 19.2 red. biolum.^ Not toxic 3.38 MPCA 1997b109* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.3 EC50 biolum.# Toxic 4.41 MPCA 1997b109* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 4.41 MPCA 1997b109* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.41 MPCA 1997b109* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.41 MPCA 1997b112* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 3.9 Crane et al. 1997112* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 3.9 Crane et al. 1997115* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c115* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c115* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c115* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.97 MPCA 1997c115* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c116* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.22 red. biolum.^ Not toxic 2.37 MPCA 1997b116* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 77.5 % survival Not toxic 2.37 MPCA 1997b116* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.57 EC50 biolum.# Not toxic 2.37 MPCA 1997b116* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.37 MPCA 1997b120 Duluth-Superior Harbor-Hallett Coal Dock #6 48-h Cladoceran Daphnia magna Unknown 95 % survival Not toxic NR Smith et al. 1992120 Duluth-Superior Harbor-North of Minnesota 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 1992120 Duluth-Superior Harbor-North of Minnesota 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR Smith et al. 1992

120 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 1996120 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 1996120 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 1996

G-92



120 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 1996120 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 1996120 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 1996120 Duluth-Superior Harbor-Hallett Coal Dock #6 10 day Diptera Chironomus tentans Unknown 86.6 % survival Not toxic NR Smith et al. 1992120 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 1996120 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 1996122* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c122* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c122* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 8.97 MPCA 1997c122* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c122* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c122* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c122* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c122* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c122* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 red. biolum.^ Toxic 4.05 MPCA 1997c122* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c124* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b124* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 red. biolum.^ Toxic 13.98 MPCA 1997b124* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b124* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b125* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c125* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c125* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c125* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic 3.87 MPCA 1997c125* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c125* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c125* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.99 MPCA 1997c125* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c125* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 10.68 MPCA 1997b125* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 10.68 MPCA 1997b125* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 14.8 red. biolum.^ Not toxic 10.68 MPCA 1997b139* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 29.84 MPCA 1997c139* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c

G-93



139* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c139* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c139* St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c143 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996143 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996143 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996143 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996145* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b145* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b145* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 red. biolum.^ Not toxic 5.34 MPCA 1997b145* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b145* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic 3 Crane et al. 1997146* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b146* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b146* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 red. biolum.^ Not toxic 5.59 MPCA 1997b146* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b149* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.24 MPCA 1997b149* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.12 EC50 biolum.# Toxic 4.24 MPCA 1997b149* Spirit Lake 10 day Amphipod Hyalella azteca JUV 41 % survival Toxic 4.24 MPCA 1997b149* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 53.2 red. biolum.^ Toxic 4.24 MPCA 1997b151* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 2.8 Crane et al. 1997151* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 3 Crane et al. 1997151* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3 Crane et al. 1997152* St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b152* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 red. biolum.^ Toxic 3.47 MPCA 1997b152* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b152* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b161* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b161* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b161* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b161* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b161* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 4.11 MPCA 1997b161* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b

G-94



161* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 6.5 Crane et al. 1997161* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 6.5 Crane et al. 1997165* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic 2.2 Crane et al. 1997165* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.2 Crane et al. 1997167* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 red. biolum.^ Not toxic 4.05 MPCA 1997b167* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b167* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b167* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b167 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 1996167 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 1996167 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 1996167 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 1996168* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b168* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b168* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 red. biolum.^ Toxic 4.54 MPCA 1997b168* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b171* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 3.99 MPCA 1997b171* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 3.99 MPCA 1997b171* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 10 % survival Toxic 3.99 MPCA 1997b171* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 66.5 red. biolum.^ Toxic 3.99 MPCA 1997b172* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b172* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b172* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b172* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 red. biolum.^ Not toxic 3.98 MPCA 1997b174* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c174* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 red. biolum.^ Toxic 4.48 MPCA 1997c174* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c176* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b176* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b176* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b176* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 red. biolum.^ Toxic 2.13 MPCA 1997b177* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.2 Crane et al. 1997177* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.2 Crane et al. 1997

G-95



178* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.6 Crane et al. 1997178* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic 6.6 Crane et al. 1997178* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b178* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b178* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b178* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 red. biolum.^ Toxic 4.13 MPCA 1997b184* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 3.76 MPCA 1997b184* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.25 EC50 biolum.# Toxic 3.76 MPCA 1997b184* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 11.1 red. biolum.^ Not toxic 3.76 MPCA 1997b184* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 44 % survival Not toxic 3.76 MPCA 1997b184* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 5.2 Crane et al. 1997184* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 5.2 Crane et al. 1997203* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 red. biolum.^ Toxic 11.975 MPCA 1997c203* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.975 MPCA 1997c203* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.975 MPCA 1997c203* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.975 MPCA 1997c203* Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.975 MPCA 1997c210 Duluth-Superior Harbor-Northeast of Western Lake 10 day Diptera Chironomus tentans Unknown 100 % survival Not toxic NR Smith et al. 1992210 Duluth-Superior Harbor-Northeast of Western Lake 48-h Cladoceran Daphnia magna Unknown 100 % survival Not toxic NR Smith et al. 1992

221* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c221* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c221* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c221* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c221* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 red. biolum.^ Toxic 6.77 MPCA 1997c229* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b229* Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b229* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b229* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 red. biolum.^ Toxic 0.82 MPCA 1997b233* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b233* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b233* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 red. biolum.^ Toxic 14.19 MPCA 1997b233* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b243* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c

G-96



243* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c243* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 red. biolum.^ Toxic 6.03 MPCA 1997c252* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 red. biolum.^ Not toxic 4.7 MPCA 1997b252* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b252* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b252* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b253 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1996258 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 1996258 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 1996258 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 1996258 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 1996278 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 1996281 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 1996315 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 1996315 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 1996315 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 1996315 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 1996357* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 red. biolum.^ Toxic 1 MPCA 1997c357* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c357* Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c357* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c357* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c409 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 1996409 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 1996409 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 1996409 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 1996423 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 1996423 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 1996423 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 1996423 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 1996452 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996452 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996452 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996

G-97



452 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996501 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 1996501 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 1996501 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 1996501 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 1996609 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 1996609 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 1996609 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 1996609 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 19961089 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR IT Corp. 19961089 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic NR IT Corp. 19961089 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic NR IT Corp. 19961089 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic NR IT Corp. 19961246 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 19961246 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 19961246 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 19961246 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 19961498 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 19961498 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 19961498 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 19961498 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 1996


G-98

Table G-8. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Nickel in the St. Louis River Area of Concern

Nickel Test Test TOC

(mg/kg) Area Duration Organism Species Life Stage End-Point Measured Result (%) Reference

0.600* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b0.600* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 red. biolum.^ Toxic 0.14 MPCA 1997b0.600* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b0.600* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b0.880* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b0.880* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08 MPCA 1997b0.880* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b0.880* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b0.880* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b0.880* Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b0.880* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b0.880* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 red. biolum.^ Toxic 0.08 MPCA 1997b0.880* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b0.880* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 red. biolum.^ Toxic 0.2 MPCA 1997b0.880* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b0.880* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 red. biolum.^ Toxic 8.26 MPCA 1997b0.894* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b0.894* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b0.894* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 0.59 MPCA 1997b1.03* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b1.03* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b1.03* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 red. biolum.^ Toxic 0.16 MPCA 1997b1.08* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 64 % survival Not toxic 0.14 MPCA 1997b1.08* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 9.08 EC50 biolum.# Not toxic 0.14 MPCA 1997b1.08* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 45.3 red. biolum.^ Toxic 0.14 MPCA 1997b1.08* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 0.14 MPCA 1997b1.14* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b1.14* Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b1.14* Allouez Bay 30 minute Bacteria Chironomus tentans NA 26.2 red. biolum.^ Toxic 0.35 MPCA 1997b1.18* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b1.18* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b

G-99




1.18* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b1.18* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 red. biolum.^ Toxic 0.24 MPCA 1997b1.21* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 81.5 red. biolum.^ Toxic 8.4 MPCA 1997b1.21* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 8.4 MPCA 1997b1.21* Spirit Lake 10 day Amphipod Hyalella azteca JUV 3 % survival Toxic 8.4 MPCA 1997b1.21* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.4 MPCA 1997b1.44* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 1.69 MPCA 1997c1.44* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c1.44* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c1.52* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 58 % survival Toxic 0.28 Crane et al. 19971.52* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.28 Crane et al. 19971.62* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c1.62* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c1.62* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 red. biolum.^ Not toxic 1.44 MPCA 1997c1.63* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b1.63* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b1.63* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 red. biolum.^ Toxic 0.4 MPCA 1997b1.63* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b1.63* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b1.63* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b1.64* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c1.64* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 1.71 MPCA 1997c1.64* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c1.64* Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c1.64* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c1.69* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b1.69* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b1.69* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 red. biolum.^ Not toxic 0.12 MPCA 1997b1.69* St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b1.73* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b1.73* Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b

G-100




1.73* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 red. biolum.^ Toxic 4.47 MPCA 1997b1.73* Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b1.88* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c1.88* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c1.88* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c1.88* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.65 MPCA 1997c1.88* Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c2.05* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 red. biolum.^ Not toxic 4.02 MPCA 1997c2.05* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c2.05* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c2.05* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c2.05* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c2.16* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c2.16* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c2.16* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 red. biolum.^ Not toxic 1.37 MPCA 1997c2.16* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c2.16* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c2.19* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 1.55 MPCA 1997b2.19* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.7 red. biolum.^ Not toxic 1.55 MPCA 1997b2.19* St. Louis Bay 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 1.55 MPCA 1997b2.22* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 red. biolum.^ Not toxic 2.98 MPCA 1997c2.22* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c2.22* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c2.22* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c2.22* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c2.23* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 red. biolum.^ Toxic 13.91 MPCA 1997c2.23* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c2.23* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c2.23* St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c2.23* St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c2.36* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.975 MPCA 1997c

G-101




2.36* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 red. biolum.^ Toxic 11.975 MPCA 1997c2.36* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.975 MPCA 1997c2.36* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.975 MPCA 1997c2.36* Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.975 MPCA 1997c2.60* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 0.89 Crane et al. 19972.60* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 0.89 Crane et al. 19972.77* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 red. biolum.^ Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c2.77* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c2.79* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c2.79* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 6.72 MPCA 1997c2.79* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c2.79* St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c2.79* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c2.85* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 0.61 MPCA 1997b2.85* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 5.36 EC50 biolum.# Not toxic 0.61 MPCA 1997b2.85* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.61 MPCA 1997b2.85* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 0.61 MPCA 1997b3.08* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.06 EC50 biolum.# Not toxic 0.81 MPCA 1997b3.08* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.9 red. biolum.^ Toxic 0.81 MPCA 1997b3.08* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic 0.81 MPCA 1997b3.08* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 0.81 MPCA 1997b3.27* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 0.86 MPCA 1997b3.27* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 0.86 MPCA 1997b

G-102




3.27* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 14.18 EC50 biolum.# Not toxic 0.86 MPCA 1997b3.27* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 0.86 MPCA 1997b3.53* Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b3.53* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b3.53* Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 red. biolum.^ Toxic 1.02 MPCA 1997b3.53* Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b3.59* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 1.85 MPCA 1997b3.59* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 14.9 red. biolum.^ Not toxic 1.85 MPCA 1997b3.59* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.75 EC50 biolum.# Not toxic 1.85 MPCA 1997b3.59* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 1.85 MPCA 1997b3.70* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 21.31 red. biolum.^ Toxic 0.68 MPCA 1997b3.70* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.68 MPCA 1997b3.70* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.83 EC50 biolum.# Not toxic 0.68 MPCA 1997b3.77* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.24 MPCA 1997c3.77* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c3.77* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c3.77* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c3.77* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c3.79* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 red. biolum.^ Toxic 4.48 MPCA 1997c3.79* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c3.79* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c4.07* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 red. biolum.^ Toxic 3.25 MPCA 1997c4.07* Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c4.07* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c4.15* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 7.1 Crane et al. 19974.15* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 7.1 Crane et al. 19974.29* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 3.2 MPCA 1997b4.29* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 3.2 MPCA 1997b4.29* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.2 MPCA 1997b4.29* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.2 MPCA 1997b4.30* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 red. biolum.^ Toxic 3.07 MPCA 1997b

G-103




4.30* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b4.30* Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b4.37* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c4.37* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 red. biolum.^ Toxic 8.97 MPCA 1997c4.37* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c4.37* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c4.37* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c4.45* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 0.56 MPCA 1997b4.45* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.12 EC50 biolum.# Not toxic 0.56 MPCA 1997b4.45* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 12 red. biolum.^ Not toxic 0.56 MPCA 1997b4.45* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 0.56 MPCA 1997b4.56* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 2.3 Crane et al. 19974.56* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.3 Crane et al. 19974.64* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c4.64* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.85 MPCA 1997c4.64* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c4.64* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c4.64* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c4.66* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.99 MPCA 1997c4.66* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c4.66* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c4.73* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 12 Crane et al. 19974.73* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 12 Crane et al. 19974.81* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b4.81* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 red. biolum.^ Not toxic 5.59 MPCA 1997b4.81* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b4.81* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b4.97* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 red. biolum.^ Not toxic 5.77 MPCA 1997b4.97* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b4.97* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b4.97* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b

G-104




4.97* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c4.97* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c4.97* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c4.97* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 red. biolum.^ Toxic 1.87 MPCA 1997c4.97* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c5.03* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b5.03* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b5.03* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 red. biolum.^ Toxic 14.19 MPCA 1997b5.03* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b5.05* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 red. biolum.^ Not toxic 1.82 MPCA 1997b5.05* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b5.05* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b5.05* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b5.29* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c5.29* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 red. biolum.^ Not toxic 2.43 MPCA 1997c5.29* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c5.30* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 red. biolum.^ Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c5.30* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 red. biolum.^ Not toxic 3.4 MPCA 1997c5.31* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.735 MPCA 1997c5.31* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.735 MPCA 1997c5.31* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.735 MPCA 1997c5.31* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.735 MPCA 1997c5.31* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.735 MPCA 1997c

G-105




5.35* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.1 MPCA 1997b5.35* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.1 MPCA 1997b5.35* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 1.1 MPCA 1997b5.35* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.1 MPCA 1997b5.60* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic 3.87 MPCA 1997c5.60* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c5.60* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c5.60* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c5.60* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c5.61* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c5.61* Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c5.61* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c5.61* Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c5.61* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 red. biolum.^ Not toxic 2.34 MPCA 1997c5.77* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c5.77* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 red. biolum.^ Toxic 4.05 MPCA 1997c5.77* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c5.77* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c5.77* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c5.78* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 red. biolum.^ Not toxic 5.34 MPCA 1997b5.78* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b5.78* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b5.78* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b5.80* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 1.75 MPCA 1997b5.80* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.75 MPCA 1997b5.80* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.87 red. biolum.^ Toxic 1.75 MPCA 1997b5.80* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 1.75 MPCA 1997b6.18* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.94 MPCA 1997b6.18* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 1.94 MPCA 1997b6.18* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 40.37 red. biolum.^ Toxic 1.94 MPCA 1997b6.18* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic 1.94 MPCA 1997b

G-106




6.19* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 1.8 Crane et al. 19976.19* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.8 Crane et al. 19976.27* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c6.27* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c6.27* St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c6.27* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c6.27* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.97 MPCA 1997c6.35* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.6 Crane et al. 19976.41* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.19 MPCA 1997b6.41* Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 2.19 MPCA 1997b6.41* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 27.4 red. biolum.^ Toxic 2.19 MPCA 1997b6.41* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 4.18 EC50 biolum.# Not toxic 2.19 MPCA 1997b6.59* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b6.59* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b6.59* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 red. biolum.^ Not toxic 4.36 MPCA 1997b6.59* St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b6.94* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 22.4 red. biolum.^ Toxic 3.14 MPCA 1997b6.94* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.14 MPCA 1997b6.94* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.14 MPCA 1997b7.00* St. Louis Bay-East of Erie Pier 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 2.9 Crane et al. 19977.00* St. Louis Bay-East of Erie Pier 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.9 Crane et al. 19977.14* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b7.14* St. Louis River-Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b7.14* St. Louis River-Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 red. biolum.^ Not toxic 4.84 MPCA 1997b7.14* St. Louis River-Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b7.25* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 2.5 Crane et al. 19977.25* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Toxic 2.5 Crane et al. 19977.37* Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b7.37* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b7.37* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b7.37* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 red. biolum.^ Toxic 0.82 MPCA 1997b

G-107




7.54* Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b7.54* Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b7.54* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 red. biolum.^ Not toxic 13.81 MPCA 1997b7.54* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b7.67* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.69 MPCA 1997b7.67* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 1 red. biolum.^ Not toxic 3.69 MPCA 1997b7.67* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.69 MPCA 1997b7.67* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.69 MPCA 1997b7.71* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.2 MPCA 1997b7.71* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.31 red. biolum.^ Not toxic 2.2 MPCA 1997b7.71* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.2 MPCA 1997b7.71* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 2.2 MPCA 1997b7.72* Spirit Lake 10 day Amphipod Hyalella azteca JUV 55 % survival Not toxic 3.32 MPCA 1997b7.72* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.1 red. biolum.^ Not toxic 3.32 MPCA 1997b7.72* Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 3.32 MPCA 1997b7.72* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 3.32 MPCA 1997b7.96* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c7.96* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c7.96* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c7.96* Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c7.96* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 red. biolum.^ Toxic 3.95 MPCA 1997c7.96* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 red. biolum.^ Toxic 3.95 MPCA 1997c7.96* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.82 red. biolum.^ Not toxic 2.34 MPCA 1997b7.96* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.34 MPCA 1997b7.96* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.34 MPCA 1997b7.96* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.28 EC50 biolum.# Toxic 2.34 MPCA 1997b8.00* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.45 EC50 biolum.# Toxic 2.88 MPCA 1997b8.00* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 2.88 MPCA 1997b8.00* St. Louis Bay 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.88 MPCA 1997b8.04* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 6.5 Crane et al. 19978.04* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 6.5 Crane et al. 1997

G-108




8.22* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 12 % survival Toxic NR MPCA 1997b8.22* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 60 % survival Not toxic NR MPCA 1997b8.22* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 2.4 EC50 biolum.# Not toxic NR MPCA 1997b8.22* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.1 red. biolum.^ Not toxic NR MPCA 1997b8.23* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.9 Crane et al. 19978.23* St. Louis Bay-South of DM & IR Taconite Storage Facility 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 2.9 Crane et al. 19978.43* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c8.43* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c8.43* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 red. biolum.^ Toxic 6.03 MPCA 1997c8.43* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 red. biolum.^ Not toxic 4.05 MPCA 1997b8.43* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b8.43* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b8.43* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b8.43* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 91 % survival Not toxic 2.67 MPCA 1997b8.43* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 2.67 MPCA 1997b8.43* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 2.67 MPCA 1997b8.43* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 31.2 red. biolum.^ Toxic 2.67 MPCA 1997b8.50* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b8.50* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b8.50* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b8.50* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 red. biolum.^ Toxic 1.42 MPCA 1997b8.50* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b8.50* Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b8.50* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b8.50* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 17.49 MPCA 1997b8.52* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5 Crane et al. 19978.52* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5 Crane et al. 19978.57* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b8.57* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 red. biolum.^ Toxic 2.63 MPCA 1997b8.57* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b8.57* St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b

G-109




8.62* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 1.46 EC50 biolum.# Not toxic 2.4 MPCA 1997b8.62* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.4 MPCA 1997b8.62* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.4 MPCA 1997b8.62* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 35.18 red. biolum.^ Toxic 2.4 MPCA 1997b8.72* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 1.35 MPCA 1997b8.72* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b8.72* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b8.72* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b8.72* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 red. biolum.^ Toxic 6.77 MPCA 1997c8.72* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c8.72* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c8.72* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c8.72* Duluth-Superior Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c9.02* Spirit Lake-Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.6 Crane et al. 19979.02* Spirit Lake-Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic 6.6 Crane et al. 19979.14* Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b9.14* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b9.14* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 red. biolum.^ Toxic 4.13 MPCA 1997b9.14* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b9.30* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 3.02 MPCA 1997b9.30* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.02 MPCA 1997b9.30* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.02 MPCA 1997b9.30* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.02 MPCA 1997b9.32* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b9.32* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 red. biolum.^ Toxic 2.13 MPCA 1997b9.32* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b9.32* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b9.45* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b9.45* St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b9.45* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 red. biolum.^ Toxic 13.98 MPCA 1997b9.45* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b

G-110




9.48* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.3 EC50 biolum.# Toxic 4.41 MPCA 1997b9.48* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.41 MPCA 1997b9.48* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.8 red. biolum.^ Not toxic 4.41 MPCA 1997b9.48* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.41 MPCA 1997b9.57* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 13.8 red. biolum.^ Not toxic 2.93 MPCA 1997b9.57* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 2.93 MPCA 1997b9.57* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 2.93 MPCA 1997b9.69* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 10.68 MPCA 1997b9.69* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 14.8 red. biolum.^ Not toxic 10.68 MPCA 1997b9.69* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 10.68 MPCA 1997b9.69* Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b9.69* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b9.69* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 2.59 MPCA 1997b9.69* Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b9.86* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 67.5 % survival Not toxic 2.82 MPCA 1997b9.86* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.82 MPCA 1997b9.86* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.23 EC50 biolum.# Toxic 2.82 MPCA 1997b9.86* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 3.17 red. biolum.^ Not toxic 2.82 MPCA 1997b9.98* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 3.07 MPCA 1997b9.98* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.2 EC50 biolum.# Toxic 3.07 MPCA 1997b9.98* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.07 MPCA 1997b9.98* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 3.07 MPCA 1997b10.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 red. biolum.^ Not toxic 4.11 MPCA 1997b10.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b10.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b10.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b10.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b10.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b10.1* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 5.2 Crane et al. 199710.1* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 78 % survival Not toxic 5.2 Crane et al. 199710.3* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 red. biolum.^ Not toxic 2.91 MPCA 1997b

G-111




10.3* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b10.3* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b10.3* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b10.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.1 EC50 biolum.# Not toxic 3.2 MPCA 1997b10.5* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 45.6 red. biolum.^ Toxic 3.2 MPCA 1997b10.5* Spirit Lake 10 day Diptera Chironomus tentans LAR 50 % survival Not toxic 3.2 MPCA 1997b10.7 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 199610.7 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 199610.7 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 199610.7 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 199610.7* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 red. biolum.^ Not toxic 3.98 MPCA 1997b10.7* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b10.7* Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b10.7* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b11.0* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.37 MPCA 1997b11.0* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 2.37 MPCA 1997b11.0* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.64 EC50 biolum.# Not toxic 2.37 MPCA 1997b11.0* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 28.9 red. biolum.^ Toxic 2.37 MPCA 1997b11.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c11.3* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 red. biolum.^ Not toxic 29.84 MPCA 1997c11.3* St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c11.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c11.3* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c11.9* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 red. biolum.^ Not toxic 4.02 MPCA 1997b11.9* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.58 EC50 biolum.# Not toxic 4.02 MPCA 1997b11.9* St. Louis Bay 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.02 MPCA 1997b11.9* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997b11.9* Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b11.9* Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b11.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b11.9* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 red. biolum.^ Not toxic 2.82 MPCA 1997b

G-112




12.2* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 3 Crane et al. 199712.2* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3 Crane et al. 199712.3* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 3.9 Crane et al. 199712.3* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 3.9 Crane et al. 199712.4* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.38 MPCA 1997b12.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.34 EC50 biolum.# Toxic 3.38 MPCA 1997b12.4* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 19.2 red. biolum.^ Not toxic 3.38 MPCA 1997b12.4* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 35 % survival Not toxic 3.38 MPCA 1997b12.8* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 3.99 MPCA 1997b12.8* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 10 % survival Toxic 3.99 MPCA 1997b12.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 66.5 red. biolum.^ Toxic 3.99 MPCA 1997b12.8* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 3.99 MPCA 1997b13.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 44 % survival Not toxic 3.76 MPCA 1997b13.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 11.1 red. biolum.^ Not toxic 3.76 MPCA 1997b13.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 3.76 MPCA 1997b13.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.25 EC50 biolum.# Toxic 3.76 MPCA 1997b13.0 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 199613.0 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 199613.0 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 199613.0 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 199613.3* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 red. biolum.^ Toxic 3.47 MPCA 1997b13.3* St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b13.3* St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b13.3* St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b13.5* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic 3 Crane et al. 199713.6* Spirit Lake 10 day Amphipod Hyalella azteca JUV 41 % survival Toxic 4.24 MPCA 1997b13.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.12 EC50 biolum.# Toxic 4.24 MPCA 1997b13.6* Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 53.2 red. biolum.^ Toxic 4.24 MPCA 1997b13.6* Spirit Lake 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.24 MPCA 1997b13.7* Lower St. Louis River 10 day Diptera Chironomus tentans LAR 77.5 % survival Not toxic 2.37 MPCA 1997b13.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.57 EC50 biolum.# Not toxic 2.37 MPCA 1997b

G-113




13.7* Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.37 MPCA 1997b13.7* Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 9.22 red. biolum.^ Not toxic 2.37 MPCA 1997b13.8* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 55 % survival Toxic 2.2 Crane et al. 199713.8* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.2 Crane et al. 199714.2* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 2.8 Crane et al. 199715.1* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c15.1* Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 red. biolum.^ Toxic 1 MPCA 1997c15.1* Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c15.1* Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c15.1* Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c16.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b16.0* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 red. biolum.^ Not toxic 4.7 MPCA 1997b16.0* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b16.0* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b16.6* Duluth-Superior Harbor-Howards Bay 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.2 Crane et al. 199716.6* Duluth-Superior Harbor-Howards Bay 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.2 Crane et al. 199717.2 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 199617.2 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 199617.2 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 199617.2 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 199617.7* Duluth-Superior Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b17.7* Duluth-Superior Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b17.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b17.7* Duluth-Superior Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 red. biolum.^ Toxic 4.54 MPCA 1997b28.7 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 199628.7 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 199628.7 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 199628.7 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 199630.4 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 199630.4 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 199630.4 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996

G-114




30.4 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 199634.4 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199634.4 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199634.4 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 199634.4 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199641.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 199641.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 199641.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 199641.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 199644.2 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 199644.2 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 199644.2 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 199644.2 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 199662.1 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 199662.1 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199662.1 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199662.1 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199665.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 199665.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 199665.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 199665.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 199672.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 199672.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 199672.6 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 199672.6 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 199673.2 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 199673.2 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 199673.2 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 199673.2 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 199693.0 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic NR IT Corp. 199693.0 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic NR IT Corp. 1996

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93.0 St. Louis River-Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR IT Corp. 199693.0 St. Louis River-Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic NR IT Corp. 1996145 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 1996145 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 1996145 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 1996145 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 1996166 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 1996166 St. Louis River-Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 1996166 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 1996166 St. Louis River-Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 1996


G-116

Table G-9. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Total Polychlorinated Biphenyls (PCBs) in the St. Louis River Area of Concern

Total PCBs(µg/kg) Area Test Duration Test Organism Species Life Stage End-Point Measured Result TOC (%) Reference

59.0 D-S Harbor - City of Superior WWTP 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 199778.5 D-S Harbor - City of Superior WWTP 10 day Amphipod Hyalella azteca JUV 79 % survival Not toxic NR Crane et al. 1997109 D-S Harbor - City of Superior WWTP 10 day Diptera Chironomus tentans LAR 62 mg growth Not toxic NR Crane et al. 1997109 D-S Harbor - City of Superior WWTP 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 1997120 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997120 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 1997134 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997145 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997145 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 1997203 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997203 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997208 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997208 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997241 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 1997241 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997259 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 1997270 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 1997283 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 1997283 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 1997326 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 1997326 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 1997346 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997396 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997424 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 1997491 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997816 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997816 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997

^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-117

Table G-10. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Total Polycyclic Aromatic Hydrocarbons (PAHs) in thSt. Louis River Area of Concern

Total PAHs (µg/kg) Area

Test Duration


TOC (%)

35.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.0835.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.0835.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 mg growth Not toxic 0.0835.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.0852.9 St. Louis River - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.9352.9 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.9352.9 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.9352.9 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.9359.9 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.3559.9 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.3559.9 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.3562.0 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.5962.0 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.5962.0 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.5968.1 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.1468.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.1468.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.1468.1 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.1481.3 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.0281.3 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.0281.3 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.0281.3 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02102 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12102 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12102 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12102 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12154 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2154 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2154 D-S Harbor 30 minute Bacteria Chironomus tentans NA 4.94 EC50 biolum.# Not toxic 0.2154 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.2273 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16273 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16273 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16

G-118



Test Duration


TOC (%)

277 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77277 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77277 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77277 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77279 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42279 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42279 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42279 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42299 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82299 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82299 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82299 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82333 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91333 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91333 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91333 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91340 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7340 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7340 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7340 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7344 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87344 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87344 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87344 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87344 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87496 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4496 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4496 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4496 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4496 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4496 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4528 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR528 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR

G-119



Test Duration


TOC (%)

529 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59529 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59529 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59529 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59534 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7534 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7534 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7534 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7534 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7534 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7534 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7534 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7534 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7534 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7604 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34604 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34604 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34604 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34604 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34675 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69675 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69675 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69689 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82689 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82689 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82689 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82725 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR725 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR748 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25748 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25748 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25773 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35773 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35

G-120



Test Duration


TOC (%)

773 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35773 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35905 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7905 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7905 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7905 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7905 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7905 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7905 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7905 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7905 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7905 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7940 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24940 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24940 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24940 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24940 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24

1002 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.841002 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.841002 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.841002 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.841536 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.851536 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.851536 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.851536 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.851536 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.851817 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.341817 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.341817 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.341817 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.341846 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.11846 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.11846 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1

G-121



Test Duration


TOC (%)

1846 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.11846 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.11846 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.11846 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.11846 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.11846 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.11846 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.11846 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.11846 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.12000 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.652000 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.652000 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.652000 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.652000 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.652489 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.982489 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.982489 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.982489 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.982489 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.982684 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.952684 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.952684 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.952684 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.952684 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.952684 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.952742 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR2742 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR2967 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR2967 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR4227 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.44227 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.44227 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.44227 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4

G-122



Test Duration


TOC (%)

4227 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.44227 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.44227 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.44227 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.44227 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.44227 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.44276 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.024276 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.024276 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.024276 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.024276 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.024296 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.374296 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.374296 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.374296 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.374296 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.374422 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR4813 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.764813 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.764813 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.764813 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.765159 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.45159 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.45159 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.45159 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.45224 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 15224 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 15224 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 15224 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 15224 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 15451 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR5568 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.115568 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11

G-123



Test Duration


TOC (%)

5568 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.115568 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.115568 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.115568 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.115680 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.245680 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.245680 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.245680 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.245695 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR5782 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR5782 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR6141 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR6688 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.436688 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.436688 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.436826 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR6826 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR7025 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR7025 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR7092 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR7092 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR7201 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR7247 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR7247 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR7349 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.547349 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.547349 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.547349 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.547504 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.447504 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.447504 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.447802 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR7802 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR

G-124



Test Duration


TOC (%)

7829 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.997829 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.997829 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.997930 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.47930 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.47930 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.47930 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.48078 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.878078 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.878078 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.878078 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.878078 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.878292 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.058292 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.058292 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.058292 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.058482 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.818482 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.818482 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.818482 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.818692 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR8692 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR8765 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.18765 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.18765 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.18765 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.18765 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.18765 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.18765 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.18765 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.18765 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.18765 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.19244 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR

G-125



Test Duration


TOC (%)

9244 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR9593 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.139593 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.139593 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.139593 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.139710 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.639710 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.639710 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.639710 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.639874 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.19874 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.19874 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.19874 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.19893 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.829893 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.829893 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.829893 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82

10199 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.4710199 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.4710199 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.4710199 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.4710370 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.9710370 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.9710370 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.9710370 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.9710370 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.9710719 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.9810719 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.9810719 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.9810719 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.9810955 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.4910955 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.4910955 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49

G-126



Test Duration


TOC (%)

10955 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.4911499 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR11499 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR11625 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.4811625 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.4811625 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.4811744 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.9711744 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.9711744 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.9711744 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.9711744 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.9712401 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.0512401 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.0512401 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.0512401 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.0512401 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.0512940 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.4712940 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.4712940 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.4712940 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.4713337 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.7713337 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.7713337 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.7713337 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.7713337 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.7714225 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.5414225 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.5414225 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.5414225 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.5414568 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.3614568 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.3614568 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.3614568 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36

G-127



Test Duration


TOC (%)

14749 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.9814749 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.9814749 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.9814749 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.9816294 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.7416294 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.7416294 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.7416294 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.7416294 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.7418737 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.1518737 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.1518737 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.1518737 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.1520340 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.7220340 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.7220340 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.7220340 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.7220340 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.7220748 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.3720748 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.3720748 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.3720748 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.3722450 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.8422450 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.8422450 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.8422450 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.8422450 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.8424200 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.8826567 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.1926567 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.1926567 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.1926567 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.1936423 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13

G-128



Test Duration


TOC (%)

36423 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.1336423 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.1336423 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.1340938 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.0840938 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.0840938 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.0840938 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.0841803 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR43411 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.5943411 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.5943411 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.5943411 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.5944342 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.0744342 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.0744342 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.0744857 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR44857 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR48194 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 10048194 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 10048194 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 10048194 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 10050690 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR52144 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.752144 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.752144 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.752144 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.756816 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.7156816 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.7156816 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.7156816 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.7156816 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.7158370 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.5863454 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03

G-129



Test Duration


TOC (%)

63454 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.0363454 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.0372623 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.4272623 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.4272623 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.4272623 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42

159070 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64159070 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64159070 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64159070 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64288267 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91288267 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91288267 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91288267 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91288267 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91314638 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR314638 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR314638 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR314638 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR607636 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12607636 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12607636 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12607636 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12705683 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95705683 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95705683 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95705683 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95788663 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV -9 mg growth Not determined 11.16788663 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16788663 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16788663 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16

1199107 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.981199107 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98

G-130



Test Duration


TOC (%)

1199107 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.981199107 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.981199107 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.981201142 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.261201142 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.261201142 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.261201142 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.263306000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12

^ = relative to control; D-S = Duluth-Superior; # = expressed as % DW sediment; red. biolum. = reduced bioluminescence; EC50 = effective concentration affecting 50% of the population.LAR = larvae; JUV = juvenile; TOC = total organic carbon; NR = not reported.

G-131

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-132

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997

G-133

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997b

G-134

Reference

MPCA 1997bMPCA 1997bAnkley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bAnkley et al. 1994Ankley et al. 1994Ankley et al. 1994

G-135

Reference

Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-136

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997MPCA 1997bMPCA 1997b

G-137

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997

G-138

Reference

MPCA 1997cMPCA 1997cMPCA 1997cWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Crane et al. 1997

G-139

Reference

Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-140

Reference

MPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-141

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-142

Reference

MPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993MPCA 1997c

G-143

Reference

MPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997c

G-144

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1993

G-145

Table G-11. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Benz(a)anthracene in the St. Louis River Area of Conc

Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest

Organism Species Life Stage End-Point Measured Result TOC (%)

3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.593.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.593.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 mg growth Not toxic 0.594.40 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.084.40 D-S Harbor - Miller & Coffee Creek Embayment 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. Biolum.^ Toxic 0.084.40 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.084.40 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.086.10 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.356.10 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.356.10 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.356.40 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.936.40 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.936.40 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.936.40 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.937.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.127.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.127.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.127.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.127.20 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.027.20 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. Biolum.^ Toxic 1.027.20 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.027.20 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.028.10 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.148.10 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.148.10 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.148.10 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.1420.3 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.220.3 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.220.3 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.220.3 D-S Harbor 30 minute Bacteria Chironomus tentans NA 4.94 EC50 biolum.# Not toxic 0.222.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.8722.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.8722.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.8722.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.8722.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.8723.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.8223.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.8223.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.8223.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. Biolum.^ Not toxic 2.8223.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42

G-132


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.5923.2 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.4223.2 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.4223.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. Biolum.^ Toxic 1.4224.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.9124.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.9124.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.9124.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.9128.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR28.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR28.0 Spirit Lake - Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.7728.0 Spirit Lake - Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.7728.0 Spirit Lake - Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.7728.0 Spirit Lake - Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.7731.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.1631.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.1631.8 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.1643.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.6943.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.6943.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.6948.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.5948.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.5948.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. Biolum.^ Not toxic 2.5948.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.5949.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.449.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.449.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.449.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.449.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.449.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4

<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7<50.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7<50.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.753.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.3453.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.3453.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.3453.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.3453.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.3456.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7

G-133


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.5956.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.756.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.756.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.756.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.756.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.756.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.756.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.756.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.774.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.8274.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.8274.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.8274.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red.biolum.^ Not toxic 1.8277.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.3577.8 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.3577.8 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.3577.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.3584.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR84.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR85.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.8485.0 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.8485.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.8485.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.2485.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.2485.0 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.8485.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.2485.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.2485.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.2498.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.2598.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC 50 biolum.# Toxic 3.2598.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25140 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85140 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85140 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85140 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85140 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85209 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34209 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34209 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34209 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34

G-134


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59213 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65213 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65213 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65213 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65213 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65222 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98222 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98222 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98222 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98222 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98260 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR260 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR270 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR270 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR333 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95333 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95333 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95333 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95333 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95333 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95345 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1345 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1345 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1345 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1345 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1359 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4359 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4359 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4359 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4359 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4359 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4359 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4359 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4359 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4359 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4362 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02362 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02362 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02362 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02

G-135


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59362 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02460 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54460 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54460 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54460 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54470 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR482 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43482 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43482 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43505 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37505 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37505 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37505 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37505 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37580 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR580 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44580 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44580 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR603 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24603 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24603 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24603 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24610 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR610 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR656 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11656 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11656 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11656 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11656 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11656 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11698 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97698 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97698 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97698 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97698 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97730 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR740 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR780 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR

G-136


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59780 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR792 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76792 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76792 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76792 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76793 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4793 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4793 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4793 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 Not toxic 6.4801 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87801 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0. 23 mg growth Toxic 3.87801 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87801 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87801 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87860 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR860 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR867 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63867 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63867 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63867 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63880 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR880 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR950 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4950 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4950 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99950 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4950 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99950 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99950 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.41000 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR1000 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR1000 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR1000 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR1071 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.821071 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.821071 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.821071 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82

G-137


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.591100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR1100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR1122 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.971122 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.971122 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.971122 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.971122 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.971124 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.481124 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.481124 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.481150 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.771150 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8% red. biolum.^ Toxic 6.771150 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.771150 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.771150 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.771201 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.051201 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.051201 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.051201 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.051205 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.811205 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.811205 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.811205 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.811226 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.981226 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.981226 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.981226 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.981243 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.471243 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.471243 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.471243 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.471268 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.11268 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.11268 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.11268 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.11278 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.051278 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.051278 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.051278 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05

G-138


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.591278 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.051286 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.841286 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.841286 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.841286 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.841286 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.841368 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.981368 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.981368 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.981368 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.981369 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.471369 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.471369 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.471369 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.471395 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.131395 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.131395 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.131395 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.131400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR1400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR1483 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.491483 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.491483 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.491483 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.491530 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.741530 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.741530 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.741530 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.741530 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.741623 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.421623 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.421623 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.421623 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.421649 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.361649 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.361649 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.361649 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.361976 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.191976 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19

G-139


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.591976 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.191976 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.192105 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.722105 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.722105 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.722105 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.722105 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.722429 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.152429 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.152429 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.152429 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.152500 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.882606 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.542606 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.542606 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.542606 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.543510 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.073510 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.073510 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.073600 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR3600 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR3612 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.373612 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.373612 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.373612 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.374100 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR4137 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.264137 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.264137 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.264137 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.264382 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.134382 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.134382 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.134382 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.134788 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.594788 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.594788 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.594788 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.594935 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71

G-140


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.594935 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.714935 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.714935 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.714935 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.715167 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.75167 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.75167 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.75167 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.75300 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR6161 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.036161 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.036161 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.036744 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.956744 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.956744 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.956744 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.956914 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.916914 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.916914 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.916914 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.916914 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.917600 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.588977 St. Louis River 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.088977 St. Louis River 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.088977 St. Louis River 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.088977 St. Louis River 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.0810117 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 10010117 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 10010117 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 10010117 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 10011111 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.6411111 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.6411111 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.6411111 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.6439106 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR39106 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR39106 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR39106 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR54893 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12

G-141


Benz(a)-anthracene

(µg/kg) AreaTest

DurationTest


3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.5954893 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.1254893 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.1254893 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12104603 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16104603 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16104603 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16104603 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16116582 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98116582 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98116582 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98116582 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98116582 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98230000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12


G-142

cern

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-143

cern

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al . 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-144

cern

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-145

cern

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al . 1997Crane et al. 1997Crane et al . 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-146

cern

Reference

MPCA 1997bMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al . 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cCrane et al . 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997

G-147

cern

Reference

MPCA 1997bCrane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997cWenck Associates Inc. 1995MPCA 1997cMPCA 1997cWenck Associates Inc. 1995Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-148

cern

Reference

MPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-149

cern

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al . 1997Crane et al . 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-150

cern

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1993IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997c

G-151

cern

Reference

MPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-152

cern

Reference

MPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993

G-153

Table G-12. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Benzo(a)pyrene in the St. Louis River Area of Concern

Benzo(a)-pyrene(µg/kg) Area Test Duration


TOC (%) Reference

3.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b3.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b3.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 mg growth Toxic 0.35 MPCA 1997b3.70 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 19963.70 St. Louis River - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 19963.70 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 19963.70 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 19964.00 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b4.00 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b4.00 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.59 MPCA 1997b4.30 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b4.30 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08 MPCA 1997b4.30 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08 MPCA 1997b4.30 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b5.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b5.50 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b5.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b5.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02 MPCA 1997b8.00 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b8.00 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b8.00 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b8.00 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14 MPCA 1997b9.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b9.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12 MPCA 1997b9.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b9.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b15.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42 MPCA 1997b15.2 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b15.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b15.2 D-S Harbor 10 day Amphipod Chironomus tentans JUV 100 % survival Not toxic 1.42 MPCA 1997b17.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c17.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87 MPCA 1997c17.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c17.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c

G-143




TOC (%) Reference

17.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c20.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 199720.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199721.6 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b21.6 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.2 MPCA 1997b21.6 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b21.6 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b28.3 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b28.3 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b28.3 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59 MPCA 1997b28.3 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b32.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b32.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b32.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b32.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82 MPCA 1997b32.5 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b32.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77 MPCA 1997b32.5 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b32.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b36.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b36.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b36.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91 MPCA 1997b36.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b36.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16 MPCA 1997b36.1 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b36.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b39.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b39.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4 MPCA 1997b39.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b39.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b39.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b39.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b

<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 1995

G-144




TOC (%) Reference

50.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c<50.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 199550.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c50.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69 MPCA 1997c71.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 199771.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR Crane et al. 199772.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34 MPCA 1997c72.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c72.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c72.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c72.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c78.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c78.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c78.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c78.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24 MPCA 1997c78.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c83.6 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b83.6 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b83.6 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b83.6 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35 MPCA 1997b88.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c88.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c104 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b104 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82 MPCA 1997b104 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b

G-145




TOC (%) Reference

104 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b106 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c106 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25 MPCA 1997c106 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c110 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b110 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b110 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b110 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84 MPCA 1997b192 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7 Ankley et al. 1994192 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7 Ankley et al. 1994196 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c196 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c196 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c196 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85 MPCA 1997c196 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c201 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c201 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c201 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65 MPCA 1997c201 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c201 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c210 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR Crane et al. 1997210 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR Crane et al. 1997230 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997230 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997233 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c233 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c

G-146




TOC (%) Reference

233 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c233 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c233 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98 MPCA 1997c248 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b248 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b248 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b248 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34 MPCA 1997b285 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1 Ankley et al. 1994285 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994320 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1 MPCA 1997c320 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c320 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c320 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c320 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c357 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c357 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c357 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c357 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c357 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b357 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b357 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54 MPCA 1997b357 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02 MPCA 1997c357 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b381 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c

G-147




TOC (%) Reference

381 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c381 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95 MPCA 1997c381 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95 MPCA 1997c381 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c381 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c422 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996422 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996422 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996422 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996437 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c437 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c437 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4 MPCA 1997c437 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c437 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c437 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c437 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c437 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c437 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c437 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4 MPCA 1997c466 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44 MPCA 1997c466 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c466 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c470 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997509 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43 MPCA 1997c509 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c509 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c522 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b522 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b522 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24 MPCA 1997b522 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b560 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c560 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c560 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37 MPCA 1997c560 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c

G-148




TOC (%) Reference

560 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c588 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996588 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996588 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996588 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996590 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 1997590 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997610 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997610 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997689 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c689 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c689 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c689 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c689 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97 MPCA 1997c732 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 1996732 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 1996732 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 1996732 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 1996750 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 1995750 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4 Wenck Associates Inc. 1995750 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997750 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 1995750 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR Crane et al. 1997750 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR Crane et al. 1997750 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 1995780 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997790 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997790 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 1997830 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997830 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 1997858 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99 MPCA 1997c858 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c858 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c860 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997

G-149




TOC (%) Reference

860 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997862 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b862 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b862 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b862 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82 MPCA 1997b867 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b867 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b867 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b867 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11 MPCA 1997b867 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b867 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 1997920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 1997925 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 1996925 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 1996925 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 1996925 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 1996940 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR Crane et al. 1997940 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997

1000 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971000 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971019 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c1019 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c1019 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87 MPCA 1997c1019 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c1019 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c1022 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c1022 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c1022 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48 MPCA 1997c1072 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c1072 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c1072 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c1072 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c1072 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84 MPCA 1997c

G-150




TOC (%) Reference

1119 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c1119 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c1119 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77 MPCA 1997c1119 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c1119 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c1190 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b1190 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98 MPCA 1997b1190 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b1190 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b1192 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b1192 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05 MPCA 1997b1192 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b1192 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b1250 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63 MPCA 1997b1250 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b1250 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b1250 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b1290 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b1290 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b1290 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b1290 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49 MPCA 1997b1300 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 19971300 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19971314 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b1314 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81 MPCA 1997b1314 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b1314 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b1322 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b1322 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b1322 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47 MPCA 1997b1322 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b1351 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b1351 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b1351 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b

G-151




TOC (%) Reference

1351 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13 MPCA 1997b1353 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47 MPCA 1997b1353 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b1353 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b1353 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b1415 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b1415 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b1415 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b1415 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98 MPCA 1997b1580 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1 Ankley et al. 19941580 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1 Ankley et al. 19941616 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c1616 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c1616 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97 MPCA 1997c1616 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c1616 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c1696 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c1696 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c1696 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c1696 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05 MPCA 1997c1696 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c1719 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c1719 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c1719 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c1719 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.72 MPCA 1997c1719 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c

G-152




TOC (%) Reference

1749 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74 MPCA 1997c1749 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74 MPCA 1997c1749 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74 MPCA 1997c1749 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74 MPCA 1997c1749 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74 MPCA 1997c1900 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b1900 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b1900 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36 MPCA 1997b1900 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b2588 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 19962588 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 19962588 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 19962588 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 19962902 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 19962902 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 19962902 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 19962902 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 19962926 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19 MPCA 1997b2926 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b2926 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b2926 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b3300 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19973300 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19973648 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b3648 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.07 MPCA 1997b3648 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b3744 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 19963744 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 19963744 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 19963744 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 19963900 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 19973900 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 19973920 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b3920 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b

G-153




TOC (%) Reference

3920 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b3920 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.59 MPCA 1997b4262 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b4262 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b4262 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b4262 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.26 MPCA 1997b4685 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b4685 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.13 MPCA 1997b4685 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b4685 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b4901 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.71 MPCA 1997c4901 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c4901 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c4901 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c4901 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c5300 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 19936226 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 19966226 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 19966226 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 19966226 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 19966383 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b6383 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b6383 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b6383 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.7 MPCA 1997b6455 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c6455 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.03 MPCA 1997c6455 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c6463 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 19966463 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 19966463 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 19966463 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 19967129 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 19967129 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 19967129 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 1996

G-154




TOC (%) Reference

7129 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19967876 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 19967876 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 19967876 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 19967876 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 19968942 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91 MPCA 1997c8942 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c8942 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c8942 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c8942 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c10000 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 199323866 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199623866 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 199623866 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199623866 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199639106 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 199639106 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 199639106 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 199639106 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 199671130 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 199671130 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199671130 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199671130 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199688090 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98 MPCA 1997c88090 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98 MPCA 1997c88090 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98 MPCA 1997c88090 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98 MPCA 1997c88090 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98 MPCA 1997c

170000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993


G-155

Table G-13. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Chrysene in the St. Louis River Area of Concern

Chrysene(µg/kg) Area

Test Duration Test Organism Species Life Stage End-Point Measured Result

TOC (%) Reference

3.70 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 19963.70 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 19963.70 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 19963.70 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 19965.60 D-S Harbor - Miller & Coffee Creek Embayment 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b5.60 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08 MPCA 1997b5.60 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b5.60 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08 MPCA 1997b7.30 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b7.30 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b7.30 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.59 MPCA 1997b8.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b8.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02 MPCA 1997b8.50 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b8.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.35 MPCA 1997b8.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b8.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b8.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b9.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b9.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b9.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12 MPCA 1997b9.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b10 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14 MPCA 1997b10 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b10 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b10 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b23 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b23 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b23 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b23 D-S Harbor 30 minute Bacteria Chironomus tentans NA 25.2 % red. biolum.^ Toxic 0.2 MPCA 1997b30 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b30 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b30 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b30 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77 MPCA 1997b33 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199733 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 1997

34 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b34 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16 MPCA 1997b

34 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b

G-156




TOC (%) Reference

35 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c35 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c35 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87 MPCA 1997c35 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c35 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c37 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b37 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82 MPCA 1997b37 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b37 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b40 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b40 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b40 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91 MPCA 1997b40 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b41 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b41 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b41 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42 MPCA 1997b41 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b

<50 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 1995<50 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 199552 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b52 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b52 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b52 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b52 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4 MPCA 1997b52 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b56 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c56 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69 MPCA 1997c56 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c59 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59 MPCA 1997b59 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b59 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b59 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b73 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c

73 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c

G-157




TOC (%) Reference

73 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c73 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c78 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c78 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c78 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c78 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c78 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34 MPCA 1997c86 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82 MPCA 1997b86 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b86 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b86 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b93 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 199793 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR Crane et al. 1997

102 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b102 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35 MPCA 1997b102 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b102 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b106 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b106 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b106 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84 MPCA 1997b106 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b107 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25 MPCA 1997c107 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c107 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c111 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c111 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c111 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c111 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24 MPCA 1997c111 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c147 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c147 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85 MPCA 1997c147 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c147 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c

147 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c191 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c191 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c191 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65 MPCA 1997c191 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c

G-158




TOC (%) Reference

191 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c224 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7 Ankley et al. 1994224 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7 Ankley et al. 1994245 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c245 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c245 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98 MPCA 1997c245 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c245 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c247 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b247 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b247 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34 MPCA 1997b247 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b304 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996304 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996304 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996304 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996330 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997330 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997340 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR Crane et al. 1997340 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR Crane et al. 1997354 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c354 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c354 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c354 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1 MPCA 1997c354 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c

364 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c364 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95 MPCA 1997c364 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c364 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c364 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c364 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95 MPCA 1997c385 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996

G-159




TOC (%) Reference

385 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996385 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996385 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996387 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1 Ankley et al. 1994387 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1 Ankley et al. 1994404 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c404 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37 MPCA 1997c404 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c404 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c404 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c467 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4 MPCA 1997c467 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c467 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c467 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c467 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c467 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c467 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c467 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c467 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4 MPCA 1997c467 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c482 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c

482 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c482 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c482 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02 MPCA 1997c482 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c556 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24 MPCA 1997b556 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b556 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b556 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b570 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997

G-160




TOC (%) Reference

632 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c632 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43 MPCA 1997c632 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c690 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997710 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44 MPCA 1997c710 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c710 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c740 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997740 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997740 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 1997761 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 1996761 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 1996761 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 1996761 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 1996772 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b772 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b772 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b772 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b772 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11 MPCA 1997b772 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b779 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b779 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b779 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b779 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54 MPCA 1997b790 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997790 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997830 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR Crane et al. 1997830 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR Crane et al. 1997920 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c

920 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c920 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 1997920 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997920 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c920 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87 MPCA 1997c940 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997940 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997960 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 1997960 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997

1000 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 1995

G-161




TOC (%) Reference

1000 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 19951000 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 19951000 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4 Wenck Associates Inc. 19951008 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82 MPCA 1997b1008 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b1008 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b1008 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b1045 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81 MPCA 1997b1045 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b1045 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b1045 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b1054 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 19961054 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 19961054 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 19961054 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 19961100 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19971100 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR Crane et al. 19971100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 19971100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 19971102 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b1102 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b1102 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b1102 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63 MPCA 1997b1114 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c1114 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c1114 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99 MPCA 1997c1200 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997

1200 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971273 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c1273 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97 MPCA 1997c1273 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c1273 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c1273 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c1336 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b1336 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98 MPCA 1997b1336 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b1336 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b1352 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b1352 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05 MPCA 1997b1352 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b

G-162




TOC (%) Reference

1352 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b1371 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48 MPCA 1997c1371 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c1371 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c1423 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b1423 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b1423 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b1423 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98 MPCA 1997b1467 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74 MPCA 1997c1467 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74 MPCA 1997c1467 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74 MPCA 1997c1467 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74 MPCA 1997c1467 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74 MPCA 1997c1513 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49 MPCA 1997b1513 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b1513 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b1513 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b1517 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b1517 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b1517 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b1517 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13 MPCA 1997b1567 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84 MPCA 1997c1567 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c1567 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c1567 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c

1567 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c1580 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b1580 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b1580 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47 MPCA 1997b1580 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b1586 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 19961586 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 19961586 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 19961586 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 19961588 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97 MPCA 1997c1588 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c1588 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c1588 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c1588 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c1588 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b

G-163




TOC (%) Reference

1588 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36 MPCA 1997b1588 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b1588 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b1604 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1 Ankley et al. 19941604 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1 Ankley et al. 19941628 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47 MPCA 1997b1628 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b1628 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b1628 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b1672 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 19961672 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 19961672 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 19961672 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 19961700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 19971700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997

1748 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c1748 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05 MPCA 1997c1748 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c1748 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c1748 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c1881 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c1881 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c1881 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c1881 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.72 MPCA 1997c1881 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c1948 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 19961948 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 19961948 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 19961948 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 19962278 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b2278 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b2278 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b

G-164




TOC (%) Reference

2278 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19 MPCA 1997b2303 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77 MPCA 1997c2303 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c2303 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c2303 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c2303 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c2728 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b2728 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.07 MPCA 1997b2728 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b2900 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 19933400 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19973400 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19973770 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 19963770 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 19963770 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 19963770 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 19963942 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.26 MPCA 1997b3942 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b3942 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b3942 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b4068 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b

4068 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b4068 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b4068 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.13 MPCA 1997b4669 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 19964669 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 19964669 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 19964669 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 19964706 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c4706 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c4706 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c4706 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c4706 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.71 MPCA 1997c4786 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b4786 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b4786 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b4786 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.59 MPCA 1997b5780 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 19965780 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19965780 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 1996

G-165




TOC (%) Reference

5780 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 19966271 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c6271 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c6271 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91 MPCA 1997c6271 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c6271 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c6410 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b6410 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b6410 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b6410 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.7 MPCA 1997b6500 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 19977800 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 19938054 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c8054 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.03 MPCA 1997c8054 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c8861 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 19968861 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 19968861 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 19968861 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 1996

9800 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 199723866 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199623866 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199623866 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 199623866 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199636313 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 199636313 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 199636313 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 199636313 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 199683682 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199683682 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 199683682 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199683682 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199688050 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98 MPCA 1997c88050 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98 MPCA 1997c88050 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98 MPCA 1997c88050 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98 MPCA 1997c88050 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98 MPCA 1997c

190000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993

G-166




TOC (%) Reference


G-167

Table G-14. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Fluoranthene in the St. Louis River Area of Concer

Fluoranthene (µg/kg) Area

Test Duration


TOC (%) Reference

6.20 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08 MPCA 1997b6.20 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b6.20 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 mg growth Not toxic 0.08 MPCA 1997b6.20 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b10.8 Allouez Bay - Miller & Coffee Creek Embayment 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b10.8 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b10.8 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.35 MPCA 1997b11.0 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 199611.0 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 199611.0 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 199611.0 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 199611.9 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b11.9 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.59 MPCA 1997b11.9 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b12.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b12.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12 MPCA 1997b12.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b12.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b12.4 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b12.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b12.4 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b12.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14 MPCA 1997b15.3 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02 MPCA 1997b15.3 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b15.3 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b15.3 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b32.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b32.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b32.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b32.0 D-S Harbor 30 minute Bacteria Chironomus tentans NA 25.2 % red. biolum.^ Toxic 0.2 MPCA 1997b53.1 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b53.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16 MPCA 1997b53.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b54.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b54.5 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b

54.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77 MPCA 1997b

G-168



Test Duration


TOC (%) Reference

54.5 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b59.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b59.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91 MPCA 1997b59.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b59.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b63.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b63.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b63.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b63.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82 MPCA 1997b69.9 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b69.9 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b69.9 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b69.9 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42 MPCA 1997b78.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c78.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c78.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c78.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c78.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87 MPCA 1997c90.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 199590.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 199590.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 199590.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7 Wenck Associates Inc. 199590.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59 MPCA 1997b90.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b90.5 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b90.5 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b92.8 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c

92.8 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c92.8 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c

G-169



Test Duration


TOC (%) Reference

95.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b95.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b95.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4 MPCA 1997b95.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b95.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b95.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b98.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 199798.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997111 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c111 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69 MPCA 1997c111 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c117 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b117 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b117 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b117 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82 MPCA 1997b127 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34 MPCA 1997c127 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c127 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c127 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c127 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c143 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25 MPCA 1997c143 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c143 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c158 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b158 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b158 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b158 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35 MPCA 1997b160 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR Crane et al. 1997160 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 1997170 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c170 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c170 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c170 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24 MPCA 1997c

170 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c181 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7 Ankley et al. 1994

G-170



Test Duration


TOC (%) Reference

181 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7 Ankley et al. 1994181 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7 Ankley et al. 1994246 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b246 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84 MPCA 1997b246 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b246 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b308 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c308 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c308 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85 MPCA 1997c308 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c308 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c335 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34 MPCA 1997b335 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b335 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b335 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b362 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1 Ankley et al. 1994362 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1 Ankley et al. 1994

362 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1 Ankley et al. 1994438 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c438 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65 MPCA 1997c438 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c

G-171



Test Duration


TOC (%) Reference

438 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c438 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c458 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c458 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c458 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95 MPCA 1997c458 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c458 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95 MPCA 1997c458 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c530 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c530 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c530 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98 MPCA 1997c530 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c530 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c640 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997640 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997670 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR Crane et al. 1997670 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR Crane et al. 1997679 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996679 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996679 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996679 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996730 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02 MPCA 1997c730 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c730 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c730 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c730 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c776 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996776 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996776 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996776 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996804 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c

804 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c804 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c804 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4 MPCA 1997c804 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4 MPCA 1997c804 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c

G-172



Test Duration


TOC (%) Reference

804 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c804 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c804 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c804 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c950 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c950 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c950 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1 MPCA 1997c950 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c950 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c970 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971080 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97 MPCA 1997c1080 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c1080 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c1080 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c1080 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c1084 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44 MPCA 1997c1084 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c1084 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c1107 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c1107 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c1107 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c1107 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37 MPCA 1997c1107 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c1118 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11 MPCA 1997b1118 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b1118 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b1118 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b1118 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b1118 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b1190 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b

1190 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b1190 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b1190 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54 MPCA 1997b1200 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 19971200 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971300 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997

G-173



Test Duration


TOC (%) Reference

1300 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19971363 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c1363 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43 MPCA 1997c1363 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c1388 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b1388 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24 MPCA 1997b1388 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b1388 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b1400 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR Crane et al. 19971400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971400 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR Crane et al. 19971437 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99 MPCA 1997c1437 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c1437 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c1560 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87 MPCA 1997c1560 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c1560 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c1560 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c1560 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c1600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 19971600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971651 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b1651 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05 MPCA 1997b1651 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b1651 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b1700 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 1997

1700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971745 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.1 Ankley et al. 1994

G-174



Test Duration


TOC (%) Reference

1745 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.1 Ankley et al. 19941745 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1 Ankley et al. 19941775 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 19961775 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 19961775 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 19961775 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 19961900 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971900 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971900 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19971900 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 19971900 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR Crane et al. 19971900 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 19971901 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13 MPCA 1997b1901 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b1901 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b1901 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b1917 St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 19961917 St. Louis River 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 19961917 St. Louis River 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 19961917 St. Louis River 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 19962048 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81 MPCA 1997b2048 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b2048 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b2048 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b2160 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b2160 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b

2160 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b2160 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49 MPCA 1997b2180 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b2180 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b2180 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b2180 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98 MPCA 1997b2200 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 19952200 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4 Wenck Associates Inc. 1995

G-175



Test Duration


TOC (%) Reference

2200 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 19952200 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 19952204 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98 MPCA 1997b2204 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b2204 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b2204 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b2223 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47 MPCA 1997b2223 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b2223 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b2223 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b2382 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b2382 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b2382 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b2382 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47 MPCA 1997b2505 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b2505 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b2505 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b2505 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82 MPCA 1997b2549 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c2549 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c2549 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77 MPCA 1997c2549 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c2549 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c2580 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c2580 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c2580 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48 MPCA 1997c2636 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74 MPCA 1997c

2636 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74 MPCA 1997c2636 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74 MPCA 1997c2636 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74 MPCA 1997c2636 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74 MPCA 1997c2708 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63 MPCA 1997b2708 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b2708 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b2708 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b2760 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 1996

G-176



Test Duration


TOC (%) Reference

2760 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 19962760 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 19962760 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 19962811 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c2811 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c2811 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c2811 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84 MPCA 1997c2811 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c2839 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 19962839 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 19962839 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 19962839 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 19962860 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b2860 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b2860 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b2860 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36 MPCA 1997b2865 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c2865 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97 MPCA 1997c2865 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c2865 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c2865 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c2900 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 19972900 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19973026 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c3026 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c3026 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c

3026 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05 MPCA 1997c3026 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c3100 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 19933348 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 19963348 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 19963348 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 19963348 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 19964130 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c4130 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c4130 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c

G-177



Test Duration


TOC (%) Reference

4130 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.72 MPCA 1997c4130 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c6710 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b6710 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b6710 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19 MPCA 1997b6710 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b7406 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b7406 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b7406 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b7406 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.13 MPCA 1997b7507 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b7507 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b7507 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.07 MPCA 1997b7800 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b7800 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b7800 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.26 MPCA 1997b7800 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b8419 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.71 MPCA 1997c8419 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c8419 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c8419 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c8419 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c8618 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 19968618 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 19968618 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 1996

8618 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 19968863 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 19968863 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 19968863 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 19968863 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19969100 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19979100 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19979100 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 19939297 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c9297 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c9297 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c

G-178



Test Duration


TOC (%) Reference

9297 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c9297 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91 MPCA 1997c9339 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 19969339 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 19969339 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 19969339 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 19969500 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 19979946 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.59 MPCA 1997b9946 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b9946 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b9946 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b12943 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b12943 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b12943 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.7 MPCA 1997b12943 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b13000 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 199716802 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c16802 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.03 MPCA 1997c16802 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c23910 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 199623910 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 199623910 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 199623910 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 1996

67039 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 199676372 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199676372 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199676372 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199676372 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 1996163180 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 1996163180 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 1996163180 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 1996163180 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 1996246002 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98 MPCA 1997c

G-179



Test Duration


TOC (%) Reference

246002 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98 MPCA 1997c246002 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98 MPCA 1997c246002 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98 MPCA 1997c246002 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98 MPCA 1997c530000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993


G-180

Table G-15. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Pyrene in the St. Louis River Area of Concern

Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

6.80 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b6.80 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08 MPCA 1997b6.80 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 mg growth Not toxic 0.08 MPCA 1997b6.80 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b9.30 St. Louis River - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 19969.30 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 19969.30 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 19969.30 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 199611.8 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b11.8 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b11.8 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.35 MPCA 1997b12.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b12.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b12.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b12.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12 MPCA 1997b12.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b12.5 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b12.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b12.5 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14 MPCA 1997b12.8 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.59 MPCA 1997b12.8 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b12.8 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b17.6 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02 MPCA 1997b17.6 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b17.6 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b17.6 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b27.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b27.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.2 MPCA 1997b27.8 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b27.8 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b47.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77 MPCA 1997b47.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b47.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b47.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b48.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16 MPCA 1997b48.2 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b

G-181


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

48.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b<50.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 199552.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b52.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91 MPCA 1997b52.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b52.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b53.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82 MPCA 1997b53.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b53.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b53.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b57.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b57.4 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b57.4 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b57.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42 MPCA 1997b60.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c60.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c60.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87 MPCA 1997c60.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c60.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c71.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 199771.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199779.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59 MPCA 1997b79.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b79.5 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b79.5 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b84.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b84.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b84.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b84.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4 MPCA 1997b84.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b84.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b95.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c

G-182


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

95.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c95.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c99.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c99.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c99.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69 MPCA 1997c105 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c105 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c105 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c105 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c105 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34 MPCA 1997c106 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82 MPCA 1997b106 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b106 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b106 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b130 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR Crane et al. 1997130 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 1997137 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c137 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c137 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25 MPCA 1997c145 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35 MPCA 1997b145 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b145 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b145 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b158 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c158 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c158 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24 MPCA 1997c158 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c158 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c190 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7 Ankley et al . 1994

G-183


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

190 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7 Ankley et al . 1994190 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7 Ankley et al . 1994196 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84 MPCA 1997b196 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b196 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b196 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b255 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c255 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c255 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c255 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c255 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85 MPCA 1997c343 Spirit Lake - Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c343 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c343 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65 MPCA 1997c343 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c343 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c351 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b351 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34 MPCA 1997b351 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b351 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b401 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1 Ankley et al . 1994

G-184


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

401 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1 Ankley et al . 1994401 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1 Ankley et al . 1994430 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c430 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95 MPCA 1997c430 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c430 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95 MPCA 1997c430 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c430 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c447 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c447 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c447 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c447 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98 MPCA 1997c447 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c480 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997480 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997523 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996523 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996523 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996523 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996580 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR Crane et al. 1997580 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR Crane et al. 1997679 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996679 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996679 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996679 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996693 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c693 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1 MPCA 1997c693 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c693 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c693 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c718 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c718 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c718 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c718 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c718 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4 MPCA 1997c718 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c

G-185


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

718 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c718 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c718 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02 MPCA 1997c718 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c718 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c718 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c718 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c718 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c718 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4 MPCA 1997c805 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37 MPCA 1997c805 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c805 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c805 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c805 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c840 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997

1000 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971031 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24 MPCA 1997b1031 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b1031 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b1031 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b1064 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b1064 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b1064 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b1064 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b1064 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b1064 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11 MPCA 1997b1099 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44 MPCA 1997c1099 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c1099 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c1100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 19971100 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19971100 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19971200 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR Crane et al. 19971200 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR Crane et al. 19971200 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971252 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43 MPCA 1997c

G-186


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

1252 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c1252 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c1296 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 19961296 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 19961296 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 19961296 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 19961300 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971300 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 19971317 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c1317 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99 MPCA 1997c1317 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c1333 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c1333 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c1333 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87 MPCA 1997c1333 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c1333 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c1340 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b1340 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b1340 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b1340 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54 MPCA 1997b1384 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c1384 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97 MPCA 1997c1384 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c1384 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c1384 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c1400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971400 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19971400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 19971400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971500 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19971500 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR Crane et al. 19971519 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b1519 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b1519 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b1519 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05 MPCA 1997b

G-187


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

1572 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 19961572 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 19961572 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 19961572 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 19961600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 19971600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 19971635 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81 MPCA 1997b1635 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b1635 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b1635 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b1689 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b1689 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b1689 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b1689 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13 MPCA 1997b1700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19971700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19971714 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49 MPCA 1997b1714 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b1714 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b1714 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b1796 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b1796 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b1796 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82 MPCA 1997b1796 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b1800 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 19951800 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4 Wenck Associates Inc. 19951800 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 19951800 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 19951826 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b1826 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b1826 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98 MPCA 1997b1826 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b1836 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.1 Ankley et al . 19941836 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1 Ankley et al. 19941836 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.1 Ankley et al . 19941836 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1 Ankley et al. 1994

G-188


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

1836 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1 Ankley et al. 19941836 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1 Ankley et al. 19941836 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.1 Ankley et al. 19941836 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.1 Ankley et al . 19941836 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1 Ankley et al. 19941836 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1 Ankley et al . 19941838 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b1838 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b1838 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b1838 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98 MPCA 1997b1946 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74 MPCA 1997c1946 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74 MPCA 1997c1946 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74 MPCA 1997c1946 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74 MPCA 1997c1946 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74 MPCA 1997c2128 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b2128 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b2128 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47 MPCA 1997b2128 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b2133 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b2133 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63 MPCA 1997b2133 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b2133 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b2191 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c2191 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c2191 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48 MPCA 1997c2212 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84 MPCA 1997c2212 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c2212 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c2212 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c2212 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c2308 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b2308 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b2308 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36 MPCA 1997b2308 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b2400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997

G-189


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

2400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 19972429 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 19962429 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 19962429 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 19962429 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 19962503 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b2503 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b2503 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b2503 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47 MPCA 1997b2505 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c2505 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c2505 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97 MPCA 1997c2505 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c2505 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c2597 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 19962597 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 19962597 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 19962597 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 19962632 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c2632 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05 MPCA 1997c2632 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c2632 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c2632 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c2819 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 19962819 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 19962819 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 19962819 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 19962933 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.72 MPCA 1997c2933 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c2933 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c2933 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c2933 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c3000 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 19933143 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c3143 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c3143 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c

G-190


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

3143 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77 MPCA 1997c3143 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c5216 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b5216 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b5216 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.07 MPCA 1997b5291 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19 MPCA 1997b5291 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b5291 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b5291 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b5412 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b5412 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.13 MPCA 1997b5412 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b5412 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b5794 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c5794 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c5794 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c5794 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c5794 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.71 MPCA 1997c5858 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b5858 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b5858 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.26 MPCA 1997b5858 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b6615 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 19966615 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 19966615 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 19966615 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 19966658 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c6658 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c6658 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c6658 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91 MPCA 1997c6658 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c6822 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 19966822 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 19966822 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 19966822 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 19966936 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 1996

G-191


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

6936 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 19966936 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19966936 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 19967200 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19977200 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19977426 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b7426 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b7426 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b7426 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.59 MPCA 1997b7700 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 19978500 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 19939800 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 19979844 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.7 MPCA 1997b9844 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b9844 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b9844 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b12481 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c12481 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.03 MPCA 1997c12481 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c19691 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 199619691 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 199619691 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 199619691 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 199650119 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199650119 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199650119 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199650119 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 199667039 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 1996

155612 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98 MPCA 1997c155612 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98 MPCA 1997c155612 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98 MPCA 1997c155612 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98 MPCA 1997c155612 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98 MPCA 1997c

G-192


Pyrene(µg/kg) Area

Test Duration



TOC (%) Reference

158996 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 1996158996 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 1996158996 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 1996158996 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 1996410000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993


G-193

Table G-16. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Anthracene in the St. Louis River Area of Concern

Anthracene(µg/kg) Area


TOC (%)

1.40 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.081.40 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.081.40 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 mg growth Toxic 0.081.40 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.082.50 Allouez Bay - Miller & Coffee Creek Embayment 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.352.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.352.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.352.60 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.142.60 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.142.60 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.142.60 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.142.70 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.592.70 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.592.70 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.593.20 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.933.20 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.933.20 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.933.20 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.933.40 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.023.40 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.023.40 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.023.40 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.024.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.124.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.124.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.124.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.124.90 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.24.90 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.24.90 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.24.90 D-S Harbor 30 minute Bacteria Chironomus tentans NA 4.94 EC50 biolum.# Not toxic 0.28.00 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.918.00 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.918.00 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.918.00 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.919.00 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82

G-194




TOC (%)

9.00 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.829.00 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.829.00 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.8210.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.7710.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.7710.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.7710.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.7711.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.8711.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.8711.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.8711.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.8711.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.8711.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.1611.8 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.1611.8 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.1612.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.5912.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.5912.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.5912.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.5912.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.4212.4 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.4212.4 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.4212.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.4213.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.713.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.713.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.713.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.713.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.713.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.713.5 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.713.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.713.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.713.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.714.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR14.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR

G-195




TOC (%)

16.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.716.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 Percent survival/normal & UV Toxic 0.716.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.716.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 Percent weight using UV light Not toxic 0.716.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 Percent survival/normal & UV Toxic 0.716.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 Percent survival/normal & UV Toxic 0.716.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.716.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.716.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.716.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.717.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.3417.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.3417.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.3417.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.3417.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.3425.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.2525.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.2525.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.2526.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR26.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.426.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR26.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.426.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.426.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.426.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.426.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.427.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.3527.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.3527.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.3527.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.3528.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.6928.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.6928.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.8228.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.6928.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82

G-196




TOC (%)

28.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.8228.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.8229.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.8429.0 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.8429.0 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.8429.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.8430.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.2430.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.2430.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.2430.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.2430.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24

<50.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7<50.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.759.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.3459.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.3459.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.3459.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.3468.9 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 Percent survival/normal & UV Not toxic 4.168.9 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.168.9 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 Percent weight using UV light Not toxic 4.168.9 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 Percent survival/normal & UV Not toxic 4.168.9 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.168.9 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 Percent weight using UV light Not toxic 4.168.9 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 Percent survival/normal & UV Not toxic 4.168.9 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.168.9 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.168.9 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.168.9 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.168.9 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 Percent weight using UV light Toxic 4.172.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.8572.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.8572.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.8572.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85

G-197




TOC (%)

72.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.8589.3 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.9589.3 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.9589.3 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.9589.3 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.9589.3 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.9589.3 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95100 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR100 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR100 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR100 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR111 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65111 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65111 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65111 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65111 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65115 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98115 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98115 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98115 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98115 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98140 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR154 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4154 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4154 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4154 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4154 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4154 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4154 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4154 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4154 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4154 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4160 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR160 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR170 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR

G-198




TOC (%)

170 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR170 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR172 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02172 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02172 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02172 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02172 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02180 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR187 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37187 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37187 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37187 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37187 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37190 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR190 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR190 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR212 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11212 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11212 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11212 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11212 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11212 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11217 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63217 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63217 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63217 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63220 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR220 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR220 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR220 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR227 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1227 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1227 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1227 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1227 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1230 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4230 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4

G-199




TOC (%)

230 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4230 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4235 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47235 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47235 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47235 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47249 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44249 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44249 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44250 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97250 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97250 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97250 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97250 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97254 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05254 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05254 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05254 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05254 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR260 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR260 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR294 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76294 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76294 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76294 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76303 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54303 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54303 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54303 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54304 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24304 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24304 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24304 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24304 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4

G-200




TOC (%)

304 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4304 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4304 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4305 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43305 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43305 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43307 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87307 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87307 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87307 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87307 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87310 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR310 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR312 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99312 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99312 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99326 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05326 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05326 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05326 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05336 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81336 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81336 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81336 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81344 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1344 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 Percent survival/normal & UV Toxic 6.1344 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1344 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 Percent weight using UV light Not toxic 6.1344 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1344 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 Percent survival/normal & UV Not toxic 6.1344 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1344 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1344 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1344 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 Percent survival/normal & UV Toxic 6.1347 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97347 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97347 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97

G-201




TOC (%)

347 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97347 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97348 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48348 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48348 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48393 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82393 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82393 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82393 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82402 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47402 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47402 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47402 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47407 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13407 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13407 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13407 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13568 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49568 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49568 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49568 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49570 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98570 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98570 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98570 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98581 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98581 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98581 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98581 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98620 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88627 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77627 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77627 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77627 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77627 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77659 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36659 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36

G-202




TOC (%)

659 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36659 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36729 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54729 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54729 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54729 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54751 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84751 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84751 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84751 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84751 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84789 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1789 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1789 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1789 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1820 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15820 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15820 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15820 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15850 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR914 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74914 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74914 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74914 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74914 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74981 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19981 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19981 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19981 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.191145 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.371145 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.371145 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.371145 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.371342 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.721342 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.721342 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.721342 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72

G-203




TOC (%)

1342 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.721400 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR1452 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.71452 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.71452 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.71452 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.71454 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.081454 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.081454 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.081454 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.081606 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.031606 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.031606 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.031800 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.582109 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.592109 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.592109 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.592109 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.592272 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.132272 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.132272 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.132272 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.132273 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.422273 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.422273 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.422273 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.422634 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.072634 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.072634 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.072685 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1002685 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 1002685 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 1002685 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 1002800 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR2800 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR3661 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.953661 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95

G-204




TOC (%)

3661 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.953661 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.953756 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.713756 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.713756 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.713756 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.713756 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.713990 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.913990 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.913990 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.913990 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.913990 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.915285 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.265285 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.265285 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.265285 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.2612658 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.6412658 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.6412658 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.6412658 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.6416760 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR16760 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR16760 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR16760 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR46025 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.1646025 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.1646025 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.1646025 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.1650119 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.1250119 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.1250119 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.1250119 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.1297366 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.9897366 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.9897366 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.9897366 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.9897366 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98170000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12

G-205




TOC (%)


G-206

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-207

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997

G-208

Reference

Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997MPCA 1997bCrane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997cMPCA 1997b

G-209

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bAnkley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-210

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997

G-211

Reference

Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cWenck Associates Inc. 1995Wenck Associates Inc. 1995

G-212

Reference

Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996

G-213

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bAnkley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997c

G-214

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1993MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997b

G-215

Reference

MPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-216

Reference

MPCA 1997cCrane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996

G-217

Reference

IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993

G-218

Reference

G-219

Table G-17. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Fluorene in the St. Louis River Area of Concern

Fluorene (µg/kg) Area

Test Duration Test Organism Species


TOC (%) Reference

0.50 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b0.50 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08 MPCA 1997b0.50 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 mg growth Not toxic 0.08 MPCA 1997b0.50 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08 MPCA 1997b0.90 D-S Harbor - Miller & Coffee Creek Embayment 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14 MPCA 1997b0.90 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b0.90 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b0.90 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b1.40 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b1.40 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b1.40 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 % red. biolum.^ Toxic 0.2 MPCA 1997b1.40 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b1.40 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b1.40 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.35 MPCA 1997b1.40 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b1.50 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 19961.50 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 19961.50 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 19961.50 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 19962.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b2.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12 MPCA 1997b2.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b2.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b2.60 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02 MPCA 1997b2.60 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b2.60 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b2.60 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b2.70 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b2.70 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b2.70 Allouez Bay 30 minute Bacteria Chironomus tentans NA 0 % red. biolum.^ Not toxic 0.59 MPCA 1997b4.00 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b4.00 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91 MPCA 1997b4.00 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b4.00 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b5.50 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b

5.50 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16 MPCA 1997b

G-207





TOC (%) Reference

5.50 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b6.00 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b6.00 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77 MPCA 1997b6.00 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b6.00 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b6.65 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b6.65 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b6.65 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b6.65 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42 MPCA 1997b8.00 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b8.00 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87 MPCA 1997c8.00 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c8.00 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c8.00 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c8.00 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c8.00 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b8.00 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b8.00 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82 MPCA 1997b8.75 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c8.75 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c9.50 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59 MPCA 1997b9.50 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b9.50 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b9.50 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b10.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b10.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b

10.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b10.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82 MPCA 1997b

G-208





TOC (%) Reference

11.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 199711.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR Crane et al. 199711.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25 MPCA 1997c11.3 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c11.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c12.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b12.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b12.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b12.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b12.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b12.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4 MPCA 1997b13.7 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7 Ankley et al. 199413.7 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7 Ankley et al. 199414.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c14.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c14.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c14.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c14.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34 MPCA 1997c14.1 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b14.1 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b14.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b14.1 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35 MPCA 1997b20.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24 MPCA 1997c20.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c20.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c

20.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c20.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c20.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69 MPCA 1997c

G-209





TOC (%) Reference

20.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c20.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c22.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b22.0 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b22.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84 MPCA 1997b22.0 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b22.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 199722.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199729.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37 MPCA 1997c29.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c29.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c29.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c29.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c30.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c30.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c30.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85 MPCA 1997c30.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c30.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c33.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65 MPCA 1997c33.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c33.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c33.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c33.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c42.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b42.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b42.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34 MPCA 1997b42.0 Spirit Lake - Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b43.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR Crane et al. 199743.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR Crane et al. 199748.8 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994

48.8 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 1994

G-210





TOC (%) Reference

48.8 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1 Ankley et al. 199448.8 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1 Ankley et al. 1994

<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7 Wenck Associates Inc. 1995<50.0 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4 Wenck Associates Inc. 1995<50.0 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4 Wenck Associates Inc. 1995<50.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4 Wenck Associates Inc. 1995<50.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.7 Wenck Associates Inc. 1995<50.0 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4 Wenck Associates Inc. 199550.9 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b50.9 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24 MPCA 1997b50.9 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b50.9 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b51.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c51.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b51.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c51.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b51.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b51.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98 MPCA 1997c51.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c51.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c51.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c51.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95 MPCA 1997c51.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95 MPCA 1997c51.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c51.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c51.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b

51.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11 MPCA 1997b51.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c51.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b58.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b58.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b

G-211





TOC (%) Reference

58.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b58.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47 MPCA 1997b59.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 199759.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 199771.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 199771.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 199774.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 199774.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 199783.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 199783.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 199786.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 199790.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c90.5 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4 MPCA 1997c91.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b91.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b91.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05 MPCA 1997b91.0 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b94.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b94.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63 MPCA 1997b94.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b94.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b97.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c

97.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c97.0 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c97.0 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97 MPCA 1997c97.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c97.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 1997

G-212





TOC (%) Reference

100 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997104 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05 MPCA 1997c104 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c104 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c104 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c104 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c110 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997110 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997110 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 1997110 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997110 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 1997110 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997123 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c123 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c123 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c123 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02 MPCA 1997c123 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c130 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR Crane et al. 1997130 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR Crane et al. 1997133 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81 MPCA 1997b133 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b133 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b133 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b140 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997140 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997140 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997153 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b153 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b153 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b

153 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13 MPCA 1997b160 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997160 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR Crane et al. 1997163 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996163 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996163 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996163 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996

G-213





TOC (%) Reference

164 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c164 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c164 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c164 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1 MPCA 1997c164 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c165 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996165 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996165 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996165 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 1996183 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 1996183 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 1996183 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 1996183 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 1996187 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c187 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c187 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99 MPCA 1997c194 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c194 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c194 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c194 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87 MPCA 1997c194 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c198 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b198 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b198 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b198 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47 MPCA 1997b211 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49 MPCA 1997b211 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b211 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b

211 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b227 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c227 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44 MPCA 1997c227 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c236 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43 MPCA 1997c236 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c236 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c238 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48 MPCA 1997c

G-214





TOC (%) Reference

238 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c238 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c243 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b243 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b243 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b243 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98 MPCA 1997b275 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b275 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82 MPCA 1997b275 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b275 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b315 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36 MPCA 1997b315 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b315 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b315 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b345 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 1996345 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 1996345 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 1996345 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 1996368 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1 Ankley et al. 1994368 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.1 Ankley et al. 1994

368 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1 Ankley et al. 1994380 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 1997383 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 1996383 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 1996383 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 1996383 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 1996384 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c384 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c384 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c

G-215





TOC (%) Reference

384 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c384 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77 MPCA 1997c398 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c398 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c398 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c398 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.72 MPCA 1997c398 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c417 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74 MPCA 1997c417 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74 MPCA 1997c417 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74 MPCA 1997c417 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74 MPCA 1997c417 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74 MPCA 1997c417 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b417 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b417 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54 MPCA 1997b417 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b440 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 1993487 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 1996487 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 1996487 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 1996487 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 1996519 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b519 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19 MPCA 1997b519 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b519 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b536 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 1996

536 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 1996536 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 1996536 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 1996539 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b539 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98 MPCA 1997b539 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b539 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b539 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 1996539 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 1996539 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 1996

G-216





TOC (%) Reference

539 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 1996550 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 1996550 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 1996550 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 1996550 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 1996581 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c581 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97 MPCA 1997c581 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c581 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c581 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c604 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b604 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b604 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.7 MPCA 1997b604 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b729 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b729 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b729 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.13 MPCA 1997b729 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b744 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.03 MPCA 1997c744 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c744 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c780 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 1997983 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c983 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c983 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c

983 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c983 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84 MPCA 1997c1125 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.59 MPCA 1997b1125 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b1125 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b1125 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b1200 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 19931436 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c1436 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c1436 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c1436 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c

G-217





TOC (%) Reference

1436 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.71 MPCA 1997c1455 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.07 MPCA 1997b1455 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b1455 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b2600 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19972600 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19973272 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91 MPCA 1997c3272 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c3272 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c3272 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c3272 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c5010 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19965010 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 19965010 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 19965010 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 19967542 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 19967542 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 19967542 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 19967542 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 19968093 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b8093 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b8093 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.26 MPCA 1997b8093 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b8578 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 1996

8578 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 19968578 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 19968578 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 1996

28640 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 199628640 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 199628640 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 199628640 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 199633473 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199633473 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 199633473 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199633473 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199678786 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98 MPCA 1997c

G-218





TOC (%) Reference

78786 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98 MPCA 1997c78786 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98 MPCA 1997c78786 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98 MPCA 1997c78786 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98 MPCA 1997c170000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993


G-219

Table G-18. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Phenanthrene in the St. Louis River Area of Concer

Phenanthrene (µg/kg) Area Test Duration Test Organism Species


TOC (%)

3.70 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.083.70 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.083.70 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 mg growth Not toxic 0.083.70 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.086.60 St. Louis River - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.936.60 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.936.60 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.936.60 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.937.90 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.147.90 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.147.90 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.147.90 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.148.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.128.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.128.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.128.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.129.20 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.359.20 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.359.20 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.3512.9 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.5912.9 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.5912.9 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.5915.2 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.0215.2 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.0215.2 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.0215.2 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.0218.3 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.218.3 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.218.3 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.218.3 D-S Harbor 30 minute Bacteria Chironomus tentans NA 25.2 % red. biolum.^ Toxic 0.221.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.7721.5 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.7721.5 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.7721.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.7735.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82

G-220




TOC (%)

35.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.8235.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.8235.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.8236.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.9136.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.9136.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.9136.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.9136.4 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.1636.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.1636.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.1641.2 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.4241.2 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.4241.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.4241.2 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.4242.5 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.5942.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.5942.5 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.5942.5 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.5943.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.8743.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.8743.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.8743.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.8743.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87

<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 6.7<50.0 Superior Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 6.7<50.0 Superior Bay 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 6.7<50.0 Superior Bay 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 6.753.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.8253.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.8253.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.8253.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.8255.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.755.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.755.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.755.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7

G-221




TOC (%)

55.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.755.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.755.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR55.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR55.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.755.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.755.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.755.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.765.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.3465.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.3465.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.3465.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.3465.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.3470.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR70.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR74.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.774.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.774.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.774.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.774.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.774.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.774.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.774.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.774.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.774.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.778.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.2578.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.2578.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.2590.4 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.3590.4 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.3590.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.3590.4 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.3596.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.6996.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.2496.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24

G-222




TOC (%)

96.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.2496.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.2496.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.2496.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.6996.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69107 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4107 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4107 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4107 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4107 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4107 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4133 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85133 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85133 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85133 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85133 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85151 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84151 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84151 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84151 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84181 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34181 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34181 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34181 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34213 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95213 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95213 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95213 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95213 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95213 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95220 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1220 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1220 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1220 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1220 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1

G-223




TOC (%)

220 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1220 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1220 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1220 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1220 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1220 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1220 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1280 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65280 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65280 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65280 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65280 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65300 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR300 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR330 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR330 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR376 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98376 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98376 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98376 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98376 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98420 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR423 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81423 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81423 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81423 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81470 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR470 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR472 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4472 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4472 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4472 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4476 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37476 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37476 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37

G-224




TOC (%)

476 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37476 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37515 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4515 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4515 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4515 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4515 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4515 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4515 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4515 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4515 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4515 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4520 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR540 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR550 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR550 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR550 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR566 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76566 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76566 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76566 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76590 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR590 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR600 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR622 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1622 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1622 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1622 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1622 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1645 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11645 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11645 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11645 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11645 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11

G-225




TOC (%)

645 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11650 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR650 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR670 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR670 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR673 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87673 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87673 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87673 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87673 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR700 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR707 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02707 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02707 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02707 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02707 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02710 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54710 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54710 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54710 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54744 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05744 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05744 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05744 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05774 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98774 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98774 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98774 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98780 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR780 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR785 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82785 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82785 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82785 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82

G-226




TOC (%)

848 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13848 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13848 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13848 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13853 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99853 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99853 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99900 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 100 % survival Not toxic 8.4900 D-S Harbor - NW side of Lakehead Dock 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 8.4900 D-S Harbor - NW side of Lakehead Dock 48 hour Cladoceran Ceriodaphnia dubia ADT 100 % survival Not toxic 8.4900 D-S Harbor - NW side of Lakehead Dock 10 day Diptera Chironomus tentans LAR 1.04 mg growth Not toxic 8.4930 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1930 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1930 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR930 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR930 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1930 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1943 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47943 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47943 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47943 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47953 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24953 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24953 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24953 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24978 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15978 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15978 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15978 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15

1000 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR1007 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.11007 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.11007 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.11007 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.11007 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1

G-227




TOC (%)

1007 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.11007 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.11007 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.11007 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.11007 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.11040 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.631040 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.631040 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.631040 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.631068 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.051068 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.051068 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.051068 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.051068 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.051093 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.971093 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.971093 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.971093 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.971093 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.971101 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.371101 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.371101 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.371101 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.371110 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.491110 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.491110 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.491110 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.491163 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.361163 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.361163 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.361163 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.361200 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.881361 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.431361 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.431361 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43

G-228




TOC (%)

1460 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.771460 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.771460 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.771460 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.771460 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.771472 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.081472 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.081472 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.081472 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.081561 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.741561 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.741561 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.741561 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.741561 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.741702 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.481702 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.481702 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.481799 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.441799 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.441799 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.441850 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.541850 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.541850 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.541850 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.542195 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.972195 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.972195 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.972195 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.972195 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.972374 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 1002374 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 1002374 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1002374 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 1002416 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.722416 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72

G-229




TOC (%)

2416 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.722416 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.722416 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.722658 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.472658 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.472658 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.472658 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.472664 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.982664 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.982664 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.982664 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.983072 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.133072 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.133072 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.133072 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.133241 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.843241 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.843241 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.843241 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.843241 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.843800 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.584500 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR4593 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.194593 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.194593 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.194593 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.195407 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.715407 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.715407 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.715407 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.715407 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.716057 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.076057 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.076057 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.076656 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42

G-230




TOC (%)

6656 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.426656 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.426656 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.426921 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.76921 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.76921 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.76921 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.77716 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.597716 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.597716 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.597716 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.598400 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR8625 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.038625 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.038625 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.038774 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.918774 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.918774 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.918774 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.918774 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.919249 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.959249 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.959249 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.959249 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95

10000 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR10000 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR15412 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.2615412 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.2615412 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.2615412 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.2621229 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR21229 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR21229 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR21229 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR26723 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64

G-231




TOC (%)

26723 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.6426723 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.6426723 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.6471130 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.1671130 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.1671130 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.1671130 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.1676372 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.1276372 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.1276372 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.1276372 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12

261748 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98261748 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98261748 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98261748 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98261748 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98670000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12


G-232

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-233

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997c

G-234

Reference

MPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997c

G-235

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cAnkley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994

G-236

Reference

Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997c

G-237

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-238

Reference

MPCA 1997bCrane et al. 1997Crane et al. 1997Crane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cCrane et al. 1997Crane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997b

G-239

Reference

MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cWenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995Wenck Associates Inc. 1995IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994

G-240

Reference

Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994Ankley et al. 1994MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1993MPCA 1997cMPCA 1997cMPCA 1997c

G-241

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997c

G-242

Reference

MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996

G-243

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bCrane et al. 1997MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996Crane et al. 1997Crane et al. 1997MPCA 1997bMPCA 1997bMPCA 1997bMPCA 1997bIT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996

G-244

Reference

IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996IT Corp. 1996MPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1997cMPCA 1993

G-245

Table G-19. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Naphthalene in the St. Louis River Area of Concern

Napthalene (µg/kg) Area

Test Duration



TOC (%) Reference

0.500 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.2 MPCA 1997b0.500 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.2 MPCA 1997b0.500 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 25.2 mg growth Toxic 0.2 MPCA 1997b0.500 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 26.3 % red. biolum.^ Toxic 0.08 MPCA 1997b0.500 D-S Harbor - Miller & Coffee Creek Embayment 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.08 MPCA 1997b0.500 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4.94 EC50 biolum.# Not toxic 0.2 MPCA 1997b0.500 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 0.08 MPCA 1997b0.500 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.08 MPCA 1997b1.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.48 EC50 biolum.# Toxic 3.47 MPCA 1997b1.00 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 94 % survival Not toxic 3.47 MPCA 1997b1.00 St. Louis Bay 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 3.47 MPCA 1997b1.00 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 20.18 % red. biolum.^ Toxic 3.47 MPCA 1997b1.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 0.59 MPCA 1997b1.50 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 0.59 MPCA 1997b1.50 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.96 EC50 biolum.# Not toxic 0.59 MPCA 1997b1.50 St. Louis River 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.93 IT Corp. 19961.50 St. Louis River 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 2.93 IT Corp. 19961.50 St. Louis River 10 day Diptera Chironomus tentans LAR 1.04 mg growth Toxic 2.93 IT Corp. 19961.50 St. Louis River 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 2.93 IT Corp. 19962.70 D-S Harbor 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 0.14 MPCA 1997b2.70 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 67.3 % red. biolum.^ Toxic 0.14 MPCA 1997b2.70 D-S Harbor 10 day Amphipod Hyalella azteca JUV 58 % survival Not toxic 0.14 MPCA 1997b2.70 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 17.83 EC50 biolum.# Not toxic 0.14 MPCA 1997b3.00 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.7 % red. biolum.^ Toxic 1.02 MPCA 1997b3.00 Allouez Bay 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic 1.02 MPCA 1997b3.00 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.81 EC50 biolum.# Not toxic 1.02 MPCA 1997b3.00 Allouez Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.02 MPCA 1997b4.00 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 4.11 MPCA 1997b4.00 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 15.5 % red. biolum.^ Not toxic 4.11 MPCA 1997b4.00 D-S Harbor 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.11 MPCA 1997b4.00 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.24 EC50 biolum.# Toxic 4.11 MPCA 1997b4.00 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 4.11 MPCA 1997b4.00 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 4.11 MPCA 1997b4.00 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.72 EC50 biolum.# Not toxic 1.42 MPCA 1997b4.00 D-S Harbor 10 day Diptera Chironomus tentans LAR 60 % survival Not toxic 1.42 MPCA 1997b

G-233



Test Duration



TOC (%) Reference

4.00 D-S Harbor 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 1.42 MPCA 1997b4.00 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 41.98 % red. biolum.^ Toxic 1.42 MPCA 1997b4.40 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 26.2 % red. biolum.^ Toxic 0.35 MPCA 1997b4.40 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 11.24 EC50 biolum.# Not toxic 0.35 MPCA 1997b4.40 Allouez Bay 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 0.35 MPCA 1997b6.50 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.36 EC50 biolum.# Toxic 4.05 MPCA 1997b6.50 D-S Harbor 10 day Amphipod Hyalella azteca JUV 67 % survival Not toxic 4.05 MPCA 1997b6.50 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 8.3 % red. biolum.^ Not toxic 4.05 MPCA 1997b6.50 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 4.05 MPCA 1997b9.10 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 87.5 % survival Not toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 65 % survival Not toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.74 % weight using UV light Not toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.65 mg growth Toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0 % survival/normal & UV Toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Not determined 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 mg growth Not toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 65 % survival/normal & UV Toxic 0.7 Ankley et al. 19949.10 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 85 % survival Not toxic 0.7 Ankley et al. 19949.20 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 22.95 % red. biolum.^ Toxic 0.16 MPCA 1997b9.20 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.16 MPCA 1997b9.20 D-S Harbor 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 0.16 MPCA 1997b11.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 0.4 MPCA 1997b11.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic 0.4 MPCA 1997b11.5 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.4 MPCA 1997b11.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 0.24 MPCA 1997b11.5 D-S Harbor 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic 0.24 MPCA 1997b11.5 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 23.05 % red. biolum.^ Toxic 0.24 MPCA 1997b11.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 0.4 MPCA 1997b11.5 D-S Harbor 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 0.4 MPCA 1997b11.5 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 39.7 % red. biolum.^ Toxic 0.4 MPCA 1997b11.5 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.24 MPCA 1997b14.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.2 % red. biolum.^ Not toxic 4.84 MPCA 1997b14.0 St. Louis River - Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.84 MPCA 1997b14.0 St. Louis River - Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 4.84 MPCA 1997b

G-234



Test Duration



TOC (%) Reference

14.0 St. Louis River - Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.09 EC50 biolum.# Toxic 4.84 MPCA 1997b17.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 28.8 % red. biolum.^ Toxic 3.25 MPCA 1997c17.5 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.25 MPCA 1997c17.5 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.42 EC50 biolum.# Toxic 3.25 MPCA 1997c27.3 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.44 mg growth Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 0.52 EC50 biolum.# Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 12 % red. biolum.^ Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 5.3 % red. biolum.^ Not toxic 2.7 MPCA 1997c27.3 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not toxic 2.7 MPCA 1997c28.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 2.82 MPCA 1997b28.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 2.82 MPCA 1997b28.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 10.2 % red. biolum.^ Not toxic 2.82 MPCA 1997b28.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.82 MPCA 1997b31.6 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.35 MPCA 1997b31.6 D-S Harbor 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic 1.35 MPCA 1997b31.6 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.35 MPCA 1997b31.6 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 1.35 MPCA 1997b36.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 100 EC50 biolum.# Not toxic 0.12 MPCA 1997b36.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.73 EC50 biolum.# Not toxic 5.77 MPCA 1997b36.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 5.77 MPCA 1997b36.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.77 MPCA 1997b36.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 6.4 % red. biolum.^ Not toxic 5.77 MPCA 1997b36.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 0.12 MPCA 1997b36.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.4 % red. biolum.^ Not toxic 0.12 MPCA 1997b36.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 0.12 MPCA 1997b45.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 2.34 MPCA 1997c45.0 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 2.34 MPCA 1997c45.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 2.34 MPCA 1997c45.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.34 MPCA 1997c45.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 2.34 MPCA 1997c

G-235



Test Duration



TOC (%) Reference

47.0 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 48 % survival Toxic 8.08 IT Corp. 199647.0 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 53 % survival Toxic 8.08 IT Corp. 199647.0 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.14 mg growth Toxic 8.08 IT Corp. 199647.0 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.8 mg growth Toxic 8.08 IT Corp. 199650.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 18.89 % red. biolum.^ Not toxic 2.91 MPCA 1997b50.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic 2.91 MPCA 1997b50.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 2.91 MPCA 1997b50.0 Lower St. Louis River 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 2.91 MPCA 1997b53.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.45 % red. biolum.^ Toxic 2.63 MPCA 1997b53.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 2.63 MPCA 1997b53.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 2.63 MPCA 1997b53.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.32 EC50 biolum.# Toxic 2.63 MPCA 1997b56.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 100 % survival Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 87.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.85 mg growth Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 72.5 % survival/normal & UV Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.83 % weight using UV light Toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 1.08 % weight using UV light Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 95 % survival/normal & UV Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.93 mg growth Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.04 mg growth Toxic 4.1 Ankley et al. 199456.2 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.05 % weight using UV light Not toxic 4.1 Ankley et al. 199458.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 199758.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 199759.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 21.8 % red. biolum.^ Toxic 1.87 MPCA 1997c59.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 1.87 MPCA 1997c59.0 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.87 MPCA 1997c59.0 D-S Harbor 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 1.87 MPCA 1997c59.0 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 2.07 EC50 biolum.# Not toxic 1.87 MPCA 1997c63.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 199763.0 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic NR Crane et al. 199763.0 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 199763.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997

G-236



Test Duration



TOC (%) Reference

65.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 2.13 MPCA 1997b65.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.47 EC50 biolum.# Toxic 2.13 MPCA 1997b65.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 2.13 MPCA 1997b65.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.6 % red. biolum.^ Toxic 2.13 MPCA 1997b68.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -2.8 % red. biolum.^ Not toxic 1.82 MPCA 1997b68.0 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 1.82 MPCA 1997b68.0 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 70 % survival Not toxic 1.82 MPCA 1997b68.0 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 3.56 EC50 biolum.# Not toxic 1.82 MPCA 1997b74.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 199774.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 199780.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.97 EC50 biolum.# Not toxic 5.34 MPCA 1997b80.0 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 18.8 % red. biolum.^ Not toxic 5.34 MPCA 1997b80.0 Spirit Lake 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 5.34 MPCA 1997b80.0 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 5.34 MPCA 1997b91.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 1.37 MPCA 1997c91.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 3.52 EC50 biolum.# Not toxic 1.37 MPCA 1997c91.0 Lower St. Louis River 10 day Diptera Chironomus tentans LAR 52 % survival Not toxic 1.37 MPCA 1997c91.0 Lower St. Louis River 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 1.37 MPCA 1997c91.0 Lower St. Louis River 15 minute Bacteria Photobacterium phosphoreum NA 8.2 % red. biolum.^ Not toxic 1.37 MPCA 1997c99.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 199799.0 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 1997104 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.1 % red. biolum.^ Not toxic 13.81 MPCA 1997b104 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 3.88 EC50 biolum.# Not toxic 13.81 MPCA 1997b104 Spirit Lake 10 day Amphipod Hyalella azteca JUV 62 % survival Not toxic 13.81 MPCA 1997b104 Spirit Lake 10 day Diptera Chironomus tentans LAR 98 % survival Not toxic 13.81 MPCA 1997b110 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997113 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 11.76 IT Corp. 1996113 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.11 mg growth Toxic 11.76 IT Corp. 1996113 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.02 mg growth Toxic 11.76 IT Corp. 1996113 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 67 % survival Not toxic 11.76 IT Corp. 1996120 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 1997120 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 1997130 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic NR Crane et al. 1997130 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 1997130 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic NR Crane et al. 1997

G-237



Test Duration



TOC (%) Reference

130 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997132 Spirit Lake 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 1.65 MPCA 1997c132 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 1.65 MPCA 1997c132 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 4.42 EC50 biolum.# Not toxic 1.65 MPCA 1997c132 Spirit Lake 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 1.65 MPCA 1997c132 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.53 mg growth Not toxic 1.65 MPCA 1997c140 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 1997140 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997140 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997146 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 2.59 MPCA 1997b146 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 2.59 MPCA 1997b146 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 2.59 MPCA 1997b146 Spirit Lake 10 day Amphipod Hyalella azteca JUV 97.5 % survival Not toxic 2.59 MPCA 1997b155 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.29 EC50 biolum.# Not toxic 1.69 MPCA 1997c155 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 12.1 % red. biolum.^ Not toxic 1.69 MPCA 1997c155 D-S Harbor 10 day Amphipod Hyalella azteca JUV 84 % survival Not toxic 1.69 MPCA 1997c157 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 92.5 % survival Not toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 82.5 % survival/normal & UV Not toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0 % survival/normal & UV Toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 0.54 mg growth Toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Oligochaete Lumbriculus variegatus ADT 75 % survival Toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 2.5 % survival/normal & UV Toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 0.08 mg growth Not determined 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Amphipod Hyalella azteca JUV 42.5 % survival Toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.8 % weight using UV light Not toxic 6.1 Ankley et al. 1994157 St. Louis Harbor 10 day Diptera Chironomus tentans LAR 0.65 mg growth Toxic 6.1 Ankley et al. 1994163 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 2.24 MPCA 1997c163 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.3 EC50 biolum.# Not toxic 2.24 MPCA 1997c163 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 2.24 MPCA 1997c163 D-S Harbor 10 day Diptera Chironomus tentans LAR 95 % survival Not toxic 2.24 MPCA 1997c163 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 2.24 MPCA 1997c179 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.85 MPCA 1997c179 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.64 mg growth Not toxic 3.85 MPCA 1997c179 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 3.82 EC50 biolum.# Not toxic 3.85 MPCA 1997c179 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.85 MPCA 1997c

G-238



Test Duration



TOC (%) Reference

179 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.85 MPCA 1997c190 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.58 mg growth Not toxic 2.98 MPCA 1997c190 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 10.5 % red. biolum.^ Not toxic 2.98 MPCA 1997c190 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 2.98 MPCA 1997c190 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.92 EC50 biolum.# Not toxic 2.98 MPCA 1997c190 D-S Harbor 10 day Amphipod Hyalella azteca JUV 97 % survival Not toxic 2.98 MPCA 1997c214 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 20.9 % red. biolum.^ Toxic 4.05 MPCA 1997c214 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.35 mg growth Not toxic 4.05 MPCA 1997c214 D-S Harbor 10 day Diptera Chironomus tentans LAR 58 % survival Not toxic 4.05 MPCA 1997c214 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.05 MPCA 1997c214 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 4.05 MPCA 1997c218 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 5.59 MPCA 1997b218 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 4 % red. biolum.^ Not toxic 5.59 MPCA 1997b218 D-S Harbor 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 5.59 MPCA 1997b218 D-S Harbor 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 5.59 MPCA 1997b228 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c228 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 23.7 % red. biolum.^ Toxic 3.95 MPCA 1997c228 Spirit Lake 10 day Amphipod Hyalella azteca JUV 70 % survival Toxic 3.95 MPCA 1997c228 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.51 EC50 biolum.# Not toxic 3.95 MPCA 1997c228 Spirit Lake 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 3.95 MPCA 1997c228 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 30.7 % red. biolum.^ Toxic 3.95 MPCA 1997c230 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997240 D-S Harbor 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 4.7 MPCA 1997b240 D-S Harbor 10 day Amphipod Hyalella azteca JUV 88 % survival Not toxic 4.7 MPCA 1997b240 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 12.7 % red. biolum.^ Not toxic 4.7 MPCA 1997b240 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 2.55 EC50 biolum.# Not toxic 4.7 MPCA 1997b247 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.97 MPCA 1997c247 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 3.97 MPCA 1997c247 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.97 MPCA 1997c247 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.97 MPCA 1997c247 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.97 MPCA 1997c290 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997310 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997310 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 1997335 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 95 % survival Not toxic 2.43 MPCA 1997c

G-239



Test Duration



TOC (%) Reference

335 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.67 EC50 biolum.# Not toxic 2.43 MPCA 1997c335 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 8.5 % red. biolum.^ Not toxic 2.43 MPCA 1997c370 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997385 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 33.03 % red. biolum.^ Toxic 4.47 MPCA 1997b385 Allouez Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.82 EC50 biolum.# Not toxic 4.47 MPCA 1997b385 Allouez Bay 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 4.47 MPCA 1997b385 Allouez Bay 10 day Diptera Chironomus tentans LAR 68 % survival Not toxic 4.47 MPCA 1997b390 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 1997400 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 1997412 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 30 % red. biolum.^ Toxic 4.54 MPCA 1997b412 D-S Harbor 10 day Diptera Chironomus tentans LAR 5 % survival Not toxic 4.54 MPCA 1997b412 D-S Harbor 10 day Amphipod Hyalella azteca JUV 69 % survival Not toxic 4.54 MPCA 1997b412 D-S Harbor 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 4.54 MPCA 1997b416 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.02 EC50 biolum.# Toxic 6.77 MPCA 1997c416 D-S Harbor 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 6.77 MPCA 1997c416 D-S Harbor 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 6.77 MPCA 1997c416 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 30.8 % red. biolum.^ Toxic 6.77 MPCA 1997c416 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 6.77 MPCA 1997c430 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 70 % survival Not toxic NR Crane et al. 1997430 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 1997451 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 67.9 % red. biolum.^ Toxic 14.19 MPCA 1997b451 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.16 EC50 biolum.# Toxic 14.19 MPCA 1997b451 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 14.19 MPCA 1997b451 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 14.19 MPCA 1997b485 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 0.82 MPCA 1997b485 Spirit Lake 10 day Amphipod Hyalella azteca JUV 59 % survival Not toxic 0.82 MPCA 1997b485 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 26.1 % red. biolum.^ Toxic 0.82 MPCA 1997b485 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 0.82 MPCA 1997b496 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.76 EC50 biolum.# Not toxic 4.02 MPCA 1997c496 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.1 % red. biolum.^ Not toxic 4.02 MPCA 1997c496 D-S Harbor 10 day Diptera Chironomus tentans LAR 57 % survival Toxic 4.02 MPCA 1997c496 D-S Harbor 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.02 MPCA 1997c496 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 4.02 MPCA 1997c530 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 1997530 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 1997

G-240



Test Duration



TOC (%) Reference

549 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 17.49 MPCA 1997b549 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 17.49 MPCA 1997b549 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 1.49 EC50 biolum.# Not toxic 17.49 MPCA 1997b549 Spirit Lake 10 day Amphipod Hyalella azteca JUV 75 % survival Not toxic 17.49 MPCA 1997b553 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.5 EC50 biolum.# Not toxic 3.99 MPCA 1997c553 D-S Harbor 10 day Amphipod Hyalella azteca JUV 86 % survival Not toxic 3.99 MPCA 1997c553 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.99 MPCA 1997c558 D-S Harbor 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 3.4 MPCA 1997c558 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.9 % red. biolum.^ Not toxic 3.4 MPCA 1997c558 D-S Harbor 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 3.4 MPCA 1997c558 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c558 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c558 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.2 % red. biolum.^ Not toxic 3.4 MPCA 1997c558 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.74 EC50 biolum.# Not toxic 3.4 MPCA 1997c558 D-S Harbor 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 3.4 MPCA 1997c558 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.49 mg growth Not toxic 3.4 MPCA 1997c558 D-S Harbor 10 day Diptera Chironomus tentans LAR 0.37 mg growth Not toxic 3.4 MPCA 1997c594 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 26.54 IT Corp. 1996594 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 83 % survival Not toxic 26.54 IT Corp. 1996594 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Toxic 26.54 IT Corp. 1996594 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.3 mg growth Not toxic 26.54 IT Corp. 1996626 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 8.6 % red. biolum.^ Not toxic 3.98 MPCA 1997b626 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 3.98 MPCA 1997b626 Spirit Lake 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 3.98 MPCA 1997b626 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.37 EC50 biolum.# Toxic 3.98 MPCA 1997b749 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.17 mg growth Toxic 45.37 IT Corp. 1996749 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 45.37 IT Corp. 1996749 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 58 % red. biolum.^ Toxic 6.03 MPCA 1997c749 D-S Harbor 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic 6.03 MPCA 1997c749 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 1.07 mg growth Toxic 45.37 IT Corp. 1996749 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 92 % survival Not toxic 45.37 IT Corp. 1996749 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 4.07 EC50 biolum.# Not toxic 6.03 MPCA 1997c767 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 20 % red. biolum.^ Toxic 4.48 MPCA 1997c767 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.29 EC50 biolum.# Toxic 4.48 MPCA 1997c767 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 16 % survival Toxic 4.48 MPCA 1997c

G-241



Test Duration



TOC (%) Reference

850 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic NR Crane et al. 1997850 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 82 % survival Not toxic NR Crane et al. 1997961 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 6.4 IT Corp. 1996961 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.4 IT Corp. 1996961 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.59 mg growth Not toxic 6.4 IT Corp. 1996961 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 6.4 IT Corp. 19961012 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.23 mg growth Toxic 3.87 MPCA 1997c1012 St. Louis Bay 10 day Diptera Chironomus tentans LAR 75 % survival Not toxic 3.87 MPCA 1997c1012 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 3.87 MPCA 1997c1012 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.41 EC50 biolum.# Toxic 3.87 MPCA 1997c1012 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.1 % red. biolum.^ Not toxic 3.87 MPCA 1997c1032 Spirit Lake 10 day Amphipod Hyalella azteca JUV 63 % survival Toxic 1 MPCA 1997c1032 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 52.4 % red. biolum.^ Toxic 1 MPCA 1997c1032 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.77 EC50 biolum.# Not toxic 1 MPCA 1997c1032 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 1 MPCA 1997c1032 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 1 MPCA 1997c1100 St. Louis River 10 day Diptera Chironomus tentans LAR 87 % survival Not toxic 5.88 MPCA 19931141 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 5.4 % red. biolum.^ Not toxic 1.44 MPCA 1997c1141 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 1.14 EC50 biolum.# Not toxic 1.44 MPCA 1997c1141 D-S Harbor 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic 1.44 MPCA 1997c1300 Spirit Lake - Grassy Point 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19971300 Spirit Lake - Grassy Point 10 day Amphipod Hyalella azteca JUV 65 % survival Toxic NR Crane et al. 19971408 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic 23.1 IT Corp. 19961408 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 23.1 IT Corp. 19961408 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 23.1 IT Corp. 19961408 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.35 mg growth Not toxic 23.1 IT Corp. 19961514 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 77 % survival Not toxic 6.15 IT Corp. 19961514 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.84 mg growth Toxic 6.15 IT Corp. 19961514 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 83 % survival Not toxic 6.15 IT Corp. 19961514 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.15 mg growth Toxic 6.15 IT Corp. 19961736 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA -0.6 % red. biolum.^ Not toxic 4.36 MPCA 1997b1736 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.96 EC50 biolum.# Not toxic 4.36 MPCA 1997b1736 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 68 % survival Not toxic 4.36 MPCA 1997b1736 St. Louis Bay 10 day Diptera Chironomus tentans LAR 85 % survival Not toxic 4.36 MPCA 1997b1970 D-S Harbor 10 day Diptera Chironomus tentans LAR 3 % survival Toxic 8.97 MPCA 1997c

G-242



Test Duration



TOC (%) Reference

1970 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 8.97 MPCA 1997c1970 D-S Harbor 15 minute Bacteria Photobacterium phosphoreum NA 0.05 EC50 biolum.# Toxic 8.97 MPCA 1997c1970 D-S Harbor 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 8.97 MPCA 1997c1970 D-S Harbor 10 day Diptera Chironomus tentans LAR Not determined Not determined 8.97 MPCA 1997c2802 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.23 mg growth Not toxic 100 IT Corp. 19962802 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 60 % survival Toxic 100 IT Corp. 19962802 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.18 mg growth Not toxic 100 IT Corp. 19962802 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 100 IT Corp. 19962831 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 6.72 MPCA 1997c2831 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 87 % survival Not toxic 6.72 MPCA 1997c2831 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.35 EC50 biolum.# Not toxic 6.72 MPCA 1997c2831 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.46 mg growth Not toxic 6.72 MPCA 1997c2831 St. Louis Bay 10 day Diptera Chironomus tentans LAR 88 % survival Not toxic 6.72 MPCA 1997c2881 St. Louis Bay 10 day Diptera Chironomus tentans LAR 82 % survival Not toxic 13.98 MPCA 1997b2881 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 56 % survival Not toxic 13.98 MPCA 1997b2881 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 13.98 MPCA 1997b2881 St. Louis Bay 30 minute Bacteria Photobacterium phosphoreum NA 29.59 % red. biolum.^ Toxic 13.98 MPCA 1997b3339 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.35 EC50 biolum.# Toxic 4.13 MPCA 1997b3339 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 4.13 MPCA 1997b3339 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 21.7 % red. biolum.^ Toxic 4.13 MPCA 1997b3339 Spirit Lake 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic 4.13 MPCA 1997b3534 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.06 EC50 biolum.# Not toxic 3.74 MPCA 1997c3534 Spirit Lake 10 day Diptera Chironomus tentans LAR 65 % survival Not toxic 3.74 MPCA 1997c3534 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0 % red. biolum.^ Not toxic 3.74 MPCA 1997c3534 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.57 mg growth Not toxic 3.74 MPCA 1997c3534 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 3.74 MPCA 1997c4100 St. Louis River 10 day Diptera Chironomus tentans LAR 93 % survival Not toxic 9.58 MPCA 19937764 St. Louis Bay 10 day Diptera Chironomus tentans LAR 45 % survival Not toxic 29.84 MPCA 1997c7764 St. Louis Bay 10 day Diptera Chironomus tentans LAR 0.43 mg growth Not toxic 29.84 MPCA 1997c7764 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic 29.84 MPCA 1997c7764 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 1.12 EC50 biolum.# Not toxic 29.84 MPCA 1997c7764 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 7.4 % red. biolum.^ Not toxic 29.84 MPCA 1997c8101 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0.07 mg growth Not determined NR IT Corp. 19968101 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 30 % survival Toxic NR IT Corp. 19968101 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 8 % survival Toxic NR IT Corp. 1996

G-243



Test Duration



TOC (%) Reference

8101 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.24 mg growth Not determined NR IT Corp. 199610000 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 32.24 % red. biolum.^ Toxic 3.07 MPCA 1997b10000 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.73 EC50 biolum.# Not toxic 3.07 MPCA 1997b10000 Spirit Lake 10 day Diptera Chironomus tentans LAR 55 % survival Not toxic 3.07 MPCA 1997b15292 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 42.1 % red. biolum.^ Toxic 1.71 MPCA 1997c15292 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 1.27 EC50 biolum.# Not toxic 1.71 MPCA 1997c15292 Spirit Lake 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic 1.71 MPCA 1997c15292 Spirit Lake 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic 1.71 MPCA 1997c15292 Spirit Lake 10 day Diptera Chironomus tentans LAR 0.4 mg growth Not toxic 1.71 MPCA 1997c21756 Spirit Lake 10 day Amphipod Hyalella azteca JUV 12 % survival Toxic 11.98 MPCA 1997c21756 Spirit Lake 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.98 MPCA 1997c21756 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.98 MPCA 1997c21756 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 84.7 % red. biolum.^ Toxic 11.98 MPCA 1997c21756 Spirit Lake 15 minute Bacteria Photobacterium phosphoreum NA 0.38 EC50 biolum.# Toxic 11.98 MPCA 1997c23431 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 11.16 IT Corp. 199623431 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 11.16 IT Corp. 199623431 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 11.16 IT Corp. 199623431 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 11.16 IT Corp. 199626723 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 4.64 IT Corp. 199626723 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 1.13 mg growth Not determined 4.64 IT Corp. 199626723 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 4.64 IT Corp. 199626723 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.16 mg growth Toxic 4.64 IT Corp. 199635714 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.56 mg growth Not determined 3.42 IT Corp. 199635714 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 38 % survival Toxic 3.42 IT Corp. 199635714 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.2 mg growth Not toxic 3.42 IT Corp. 199635714 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 52 % survival Toxic 3.42 IT Corp. 1996

147971 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV Not determined Not determined 50.12 IT Corp. 1996147971 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 8 % survival Toxic 50.12 IT Corp. 1996147971 St. Louis River - Stryker Bay 10 day Diptera Chironomus tentans LAR 0.09 mg growth Not determined 50.12 IT Corp. 1996147971 St. Louis River - Stryker Bay 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 50.12 IT Corp. 1996228613 St. Louis Bay 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic 13.91 MPCA 1997c228613 St. Louis Bay 10 day Diptera Chironomus tentans LAR Not determined Not determined 13.91 MPCA 1997c228613 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 93.4 % red. biolum.^ Toxic 13.91 MPCA 1997c228613 St. Louis Bay 10 day Diptera Chironomus tentans LAR 18 % survival Toxic 13.91 MPCA 1997c228613 St. Louis Bay 15 minute Bacteria Photobacterium phosphoreum NA 0.4 EC50 biolum.# Toxic 13.91 MPCA 1997c

G-244



Test Duration



TOC (%) Reference

440000 St. Louis River 10 day Diptera Chironomus tentans LAR 63 % survival Toxic 6.12 MPCA 1993616570 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 30 % survival Toxic 12.95 IT Corp. 1996616570 St. Louis River - Keene Creek 10 day Diptera Chironomus tentans LAR 0.65 mg growth Not determined 12.95 IT Corp. 1996616570 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 75 % survival Toxic 12.95 IT Corp. 1996616570 St. Louis River - Keene Creek 10 day Amphipod Hyalella azteca JUV 0.1 mg growth Toxic 12.95 IT Corp. 19961137000 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 0.06 EC50 biolum.# Toxic 8.26 MPCA 1997b1137000 Spirit Lake 30 minute Bacteria Photobacterium phosphoreum NA 99.9 % red. biolum.^ Toxic 8.26 MPCA 1997b1137000 Spirit Lake 10 day Diptera Chironomus tentans LAR 0 % survival Toxic 8.26 MPCA 1997b1137000 Spirit Lake 10 day Amphipod Hyalella azteca JUV 0 % survival Toxic 8.26 MPCA 1997b


G-245

Table G-20. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Toxic Equivalents (TEQs) for Wildlife in the St. Louis River Area of Concern

TEQ(µg/kg) Area



TOC (%) Reference

0.008 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 19970.011 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19970.011 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 mg growth Not toxic NR Crane et al. 19970.012 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19970.012 D-S Harbor - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19970.012 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 19970.032 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 19970.043 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19970.043 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 19970.076 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 19970.076 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 19970.086 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 19970.086 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19970.102 D-S Harbor - City of Superior WWTP 10 day Amphipod Hyalella azteca JUV 79 % survival Not toxic NR Crane et al. 19970.106 D-S Harbor - City of Superior WWTP 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.109 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19970.109 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19970.159 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.232 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19970.232 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 19970.259 D-S Harbor - City of Superior WWTP 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic NR Crane et al. 19970.259 D-S Harbor - City of Superior WWTP 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 19970.259 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19970.259 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19970.312 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.379 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.396 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997


G-246

Table G-21. A Summary of the Available Data on the Biological Effects Associated with Sediment-sorbed Toxic Equivalents (TEQs) for Human Health in the St. Louis River Area of Concern

TEQ(µg/kg) Area Test Duration Test Organism Species Life Stage

End-Point Measured Result TOC (%) Reference

0.008 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 89 % survival Not toxic NR Crane et al. 19970.011 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19970.011 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 72 % survival Not toxic NR Crane et al. 19970.012 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 92 % survival Not toxic NR Crane et al. 19970.012 D-S Harbor - Slip C 10 day Diptera Chironomus tentans LAR 55 % survival Toxic NR Crane et al. 19970.012 D-S Harbor - Minnesota Slip 10 day Amphipod Hyalella azteca JUV 100 % survival Not toxic NR Crane et al. 19970.023 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 93 % survival Not toxic NR Crane et al. 19970.043 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 90 % survival Not toxic NR Crane et al. 19970.043 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19970.076 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 98 % survival Not toxic NR Crane et al. 19970.076 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 78 % survival Not toxic NR Crane et al. 19970.086 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 85 % survival Not toxic NR Crane et al. 19970.086 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 68 % survival Toxic NR Crane et al. 19970.102 D-S Harbor - city of Superior WWTP 10 day Amphipod Hyalella azteca JUV 79 % survival Not toxic NR Crane et al. 19970.106 D-S Harbor - city of Superior WWTP 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.109 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19970.109 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19970.156 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.223 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 72 % survival Not toxic NR Crane et al. 19970.223 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19970.259 D-S Harbor - city of Superior WWTP 10 day Diptera Chironomus tentans LAR 62 % survival Not toxic NR Crane et al. 19970.259 D-S Harbor - city of Superior WWTP 10 day Amphipod Hyalella azteca JUV 80 % survival Not toxic NR Crane et al. 19970.259 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Diptera Chironomus tentans LAR 80 % survival Not toxic NR Crane et al. 19970.259 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 90 % survival Not toxic NR Crane et al. 19970.307 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.379 D-S Harbor - Slip C 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 19970.390 St. Louis Bay - Miller & Coffee Creek Embayment 10 day Amphipod Hyalella azteca JUV 96 % survival Not toxic NR Crane et al. 1997


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