「生体超分子構造」

A01：X 線及び電子線による超分子の構造解析法の研究

月原冨武 1. Shoji Maeda, So Nakagawa, Michihiro Suga, Eiki Yamashita, Atsunori Oshima, Yoshinori Fujiyoshi, Tomitake Tsukihara* (2009) Structure of the connexin-26 gap junction channel at 3.5 Å resolution. Nature, in press

2. K. Sakurai, H. Shimada, T. Hayashi and T. Tsukihara* (2009) Substrate binding induces structural changes in cytochrome p450cam. Acta Cryst. F, 65, 80-83

3. H. Aoyama, K. Muramoto, K. Shinzawa-Itoh, K. Hirata, E. Yamashita, T. Tsukihara, T. Ogura and S. Yoshikawa* (2009) A peroxide bridge between Fe and Cu ions in the O2 reduction site of fully oxidized cytochrome c oxidase could suppress the proton punp. Proc. Natl. Acad. Sci. USA., 106, 2165-2169

4. H. Tanaka, K. Kato, E. Yamashita, T. Sumizawa, Y. Zhou, M. Yao, K. Iwasaki, M. Yoshimura and T. Tsukihara* (2009) The structure of rat liver vault at 3.5 angstrom resolution. Science, 323, 384-388

5. T. Yabe, E. Yamashita, A. Kikuchi, K. Morimoto, A. Nakagawa, T. Tsukihara and M. Nakai* (2008) Structural analysis of Arabidopsis CnfU protein: an iron-sulfur cluster biosynthetic scafold in chloroplasts. J. Mol. Biol., 381, 160-173

6. Koji Kato, Hideaki Tanaka, Tomoyuki Sumizawa, Masato Yoshimura, Eiki Yamashita, Kenji Iwasaki, Tomitake Tsukihara* (2008) A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry. Acta Crystallogr. D 64, 525-531

7. Se-Young Son, Jichun Ma, Youhei Kondou, Masato Yoshimura, Eiki Yamashita, and Tomitake Tsukihara* (2008) Structure of human monoamine oxidase at 2.2 Å resolution: The control of opening the entry for substrates/inhibitors. Proc. Natl. Acad. USA, 105, 5739-5744

［新聞報道］

• 朝日新聞（朝刊・大阪版）2009 年（平成 21 年）1月 16 日

• 朝日新聞（夕刊・東京版）2009 年（平成 21 年）1月 16 日

• 神戸新聞（朝刊）2009 年（平成 21 年）1月 16 日

• 日経産業新聞（朝刊）2009 年（平成 21 年）1月 16 日

• 産経新聞（朝刊・大阪版）2009 年（平成 21 年）1月 19 日

• 読売新聞（朝刊・大阪版）2009 年（平成 21 年）1月 26 日

難波啓一 1. Kojima, S., Furukawa, Y., Matsunami, H., Minamino, T. & Namba, K. (2008) Characterization of the Periplasmic Domain of MotB and Implications for Its Role in the Stator Assembly of the Bacterial Flagellar Motor. J. Bacteriol. 190, 3314-3322.

2. Kojima, S., Shinohara, A., Terashima, H., Yakushi, T., Sakuma, M., Homma, M., Namba, K., Imada, K. (2008) Insights into the stator assembly of the

Vibrio flagellar motor from the crystal structure of MotY. PNAS 105, 7696-7701.

3. Che,Y-S., Nakamura, S., Kojima, S., Kami-ike, N., Namba, K. & Minamino, T. (2008) Suppressor analysis of the MotB(D33E) mutation to probe the bacterial flagellar motor dynamics coupled with proton translocation. J. Bacteriol. 190, 6660-6667.

4. Tanaka, S., Miyata, T., Kato, T., Namba, K., Yanagida, T., Sako, Y., Kawata, S. & Inoue, Y. (2008) Construction of two color semiconductor quantum dots wire by utilizing the complementarity of DNA. AIP Cpnf. Proc. 1062, 116-122.

5. Iwasaki, K., Miyazaki, N., Hammar L., Zhu, Y., Omura, T., Wu, B., Sjöborg, F., Yonekura, K., Murata, K., Namba, K., Casper, D.L., Fujiyoshi, Y. & Cheng, R.H. (2008) Pleomorphic configuration of the trimeric capsid proteins of rice dwarf virus that allows formation of both the outer capsid and tubular crystals. J. Mol. Biol. 383, 252-265.

6. Minamino, T., Imada, K. & Namba, K. (2008) Mechanisms of type III protein export for bacterial flagellar assembly. Mol. BioSyst. 4, 1105-1115.

7. Minamino, T., Imada, K. & Namba, K. (2008) Molecular motors of the bacterial flagella. Current Opinion in Structural Biology, 18, 693-701.

8. Kikuchi, Y. , Matsunami, H., Yamane, M., Imada, K. & Namba, K. (2009) Crystallization and preliminary X-ray analysis of a C-terminal fragment of FlgJ, a putative flagellar rod cap protein of Salmonella. Acta Crystallogr. F65, 17-20.

9. Ibuki, T., Shimada, M., Minamino, T., Namba, K. & Imada, K. (2009) Crystallization and preliminary X-ray analysis of FliJ, a cytoplasmic component of the flagellar type III protein export apparatus from Salmonella. Acta Crystallogr. F65, 47-50.

10. Nakamura, S., Kami-ike, N., Yokota, J.P., Kudo, S., Minamino, T., & Namba, K. (2009) Effect of Intracellular pH on the Torque-Speed Relationship of Bacterial Proton-Driven Flagellar Motor. J. Mol. Biol. in press

11. 南野徹 & 難波啓一. (2008) 細菌べん毛タンパク質輸送における

ATPase とプロトン駆動力の役割分担, 細胞工学, Vol. 27, No. 5,

pp476-477.

12. 南野徹, 守屋奈緒, 難波啓一. (2008) K くんの地道な足取り, 生物物

理, Vol. 48, No. 2, pp135-136.

13. 難波啓一. (2008) 生体超分子ネットワークの仕組みに学ぶ, 学術の

動向, 第 13 巻第 5号, pp50-51.

［新聞報道］

• 科学新聞 2009 年 1 月 1 日

佐藤主税 1. Mio,K., Ogura,T., Yamamoto,T., Hiroaki,Y., Fujiyoshi,Y., Kubo,Y., Sato.C. (2009) Reconstruction of the P2X2 receptor revealed a vase-shaped structure with lateral tunnels above the membrane. Structure 17, 266-275

2. Omura,A., Matsuzaki,T., Mio,K., Ogura,T., Yamamoto,M., Fujita,A., Okawa,K., Kitayama,H. Takahashi,C. Sato,C. (2009) M.Noda. RECK forms cowbell-shaped dimers and inhibits MMP -catalyzed cleavage of fibronectin. J. Biol. Chem. 284(6), 3461-3469

3. Mio,K., Ogura,T., Mio,M., Shimizu,H., Hwang,TC., Sato,C., Sohma,Y.(2008) Three-dimensional reconstruction of human CFTR chloridechannel revealed an ellipsoidal structure with orifices beneath theputative transmembrane domain. J. Biol. Chem. 283(44), 30300-30310

4. Mio, K., Ogura, T., Sato, C. (2008) Structure of six-transmembrane cation channels revealed by single-particle analysis from EM images. J. Synchrotron Radiation. 15(3), 211-214

5. 小椋俊彦，三尾和弘，佐藤主税（2008）電顕画像からの自動３次元再

構成法の開発と TRP チャネルの構造、顕微鏡 43 巻 １号、40-45

北尾彰朗 1. Chng, C. P., Kitao, A., Thermal Unfolding Simulations of Bacterial Flagellin: Insight into its Refolding before Assembly. Biophys. J. 94, 3858-3871

2. Joti, Y., Nakagawa, H., Kataoka, M., Kitao, A., Hydration effects on low-frequency protein dynamics observed in simulated neutron scattering spectra. Biophys. 94, 4435-4443

3. Nakagawa, H., Joti, Y., Kitao, A., Kataoka, M., Hydration affects both harmonic and anharmonic nature of protein dynamics. Biophys. 95, 2916-2923

4. Sakuraba,S., Kitao, A., Multiple Markov transition matrix method: Obtaining the stationary probability distribution from multiple simulations , J. Comp. Chem. published on line.

5. Yang, L.-W., Eyal, E., Bahar, I., Kitao, A., Principal Component Analysis of Native Ensembles of Biomolecular Structures (PCA_NEST): Insights into Functional Dynamics. Bioinformatics, published on line.

6. Nishima, W., Qi, G., Hayward, S., Kitao, A., DTA: Dihedral transition analysis for characterization of the effects of large main-chain dihedral changes in proteins. Bioinformatics, published on line.

姚 閔 1. Hideaki Tanaka, Koji Kato, Eiki Yamashita, Tomoyuki Sumizawa, Yong Zhou, Min Yao, KenjiIwasaki, Masato Yoshimura, Tomitake Tsukihara. (2009) The Structure of Rat Liver Vault at 3.5 Angstrom Resolution. Science. 323, 384-388.

2. Xiaodong Zhang, Takashi Nakashima, Yoshimitsu Kakuta, Min Yao, Isao Tanaka, and Makoto Kimura. (2008) Crystal structure of an archaeal Ski2p-like protein from Pyrococcus horikoshii OT3. Protein Sci. 17,

136-145. 3. Makoto Kuroda, Ryuta Ito, Yoshikazu Tanaka, Min Yao, Kimio Matoba,

Shinji Saito, Isao Tanaka, Toshiko Ohta. (2008) Staphylococcus aureus Surface Protein SasG Contributes to Intercellular Autoaggregation of Staphylococcus aureus. Biochem. Biophys. Res. Commun. 377, 1102-1106.

4. Parvin Begum, Naoki Sakai, Takeshi Hayashi, Yong-Gui Gao, Tomohiro Tamura, Nobuhisa Watanabe1, Min Yao and Isao Tanaka. (2008) Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2). Protein & Peptide Letters, 15, 709-712.

5. Y.-G. Gao, H.Suzuki, H.Itou, Y. Zhou, Y. Tanaka, M. Wachi, N. Watanabe, I. Tanaka and M. Yao. (2008) Structural and Functional Characterization of the LldR from Corynebacterium glutamicum: a Transcriptional Repressor Involved in l-Lactate and Sugar Utilization. Nucleic Acid Research, 36, 22, 7110-7123.

6. Momoyo Kitamura, Masayuki Okuyama, Fumiko Tanzawa, Haruhide Mori, Yu Kitago,Nobuhisa Watanabe, Atsuo Kimura, Isao Tanaka and Min Yao. (2008) Structural and Functional Analysis of a Glycoside Hydrolase Family 97 Enzyme from Bacteroides thetaiotaomicron. J. Biol. Chem. 283, 36328-36337.

7. Sou Sakamoto, Yoshikazu Tanaka, Isao Tanaka, Toshiaki Takei, Jian Yu, Makoto Kuroda, Min Yao, Toshiko Ohta, and Kouhei Tsumoto. (2008) Electron Microscopy and Computational Studies of Ebh, a Giant Cell-wall-associated Protein from Staphylococcus aureus. Biochem. Biophys. Res. Commun. 376, 261-266.

8. Masato Watanabe, Yoshikazu Tanaka, Ayuko Suenaga, Makoto Kuroda, Min Yao, Nobuhisa Watanabe, Fumio Arisaka, Toshiko Ohta, Isao Tanaka and Kouhei Tsumoto. (2008) Structural basis for multimeric heme complexation through a specific protein-heme interaction: the case of the third NEAT domain of IsdH from Staphylococcus aureus. J. Biol. Chem. 283, 28649-28659.

9. Yoshikazu Tanaka, Sou Sakamoto, Makoto Kuroda, Shuichiro Goda, Yong-Gui Gao, Kouhei Tsumoto, Yuzuru Hiragi, Min Yao, Nobuhisa Watanabe, Toshiko Ohta, and Isao Tanaka. (2008) A Helical String of Alternately Connected Two Three-helix Bundles for the 1.1-Megadalton Cell Wall-Associated Adhesion Protein Ebh from Staphylococcus aureus. Structure, 16, 488-496.

10. Yong-Gui Gao, Min Yao, and Isao Tanaka. (2008) Structure of protein PH0536 from P. horikoshii at 1.7 Å resolution reveals a novel assembly of an oligonucleotide/oligosaccharide-binding fold and an α-helical bundle. Proteins, 71, 503-508.

11. Hiroshi Itou, Min Yao, Nobuhisa Watanabe, and Isao Tanaka. (2008) Crystal structure of the PH1932 protein, a unique archaeal ArsR type winged-HTH transcription factor from Pyrococcus horikoshii OT3. Proteins, 70, 1631-1634.

12. U. Okada, K. Kondo, T. Hayashi, N. Watanabe, M. Yao, T. Tamurad and I. Tanaka. (2008) Structural and functional analysis of TetR family transcriptional regulator SCO0332 from Streptomyces coelicolor. Acta. Cryst. D64, 198-205.

13. SongQing Hu, YongGui Gao, Kenji Tajima, Min Yao, Takanori, Yoda, Daisuke Shimura, Yasuharu Satoh, Shin Kawano, Isao Tanaka and Masanobu Munekata. (2008) Purification, Crystallization and Preliminary X-Ray Studies of AxCesD Required for Efficient Cellulose Biosynthesis in Acetobacter xylinum. Protein Peptide Letter, 15, 115-117.

14. 姚閔 (2008) 分子モデル構築と構造精密化を行う新ソフトウェアの開

発，日本結晶学会. 50, 218-223.

15. 田中勲，渡邉信久，姚閔，坂井直樹 (2008) 特集 タンパク 3000 プロ

ジェクトの産んだもの,第 I 部 成果とインパクト：個別的解析拠点の

研究成果 転写・翻訳 翻訳系蛋白質を中心に ‘Team transcription &

translation’ aiming at 100 structures, 蛋白質 核酸 酵素. 53(5),

608-611 大澤匡範 1. Sugiki T., Shimada I., Takahashi H. (2008) Stable isotope labeling of protein

by Kluyveromyces lactis for NMR study. J Biomol NMR. 42, 159-162. 2. Sakakura M., Oo-Puthinan S., Moriyama C., Kimura T., Moriya J., Irimura

T., Shimada I. (2008) Carbohydrate binding mechanism of the macrophage galactose-type C-type lectin 1 revealed by saturation transfer experiments. J Biol Chem. 283, 33665-33673.

3. Sugahara K.N., Hirata T., Tanaka T., Ogino S., Takeda M., Terasawa H., Shimada I, Tamura J, ten Dam GB, van Kuppevelt TH, Miyasaka M. (2008) Chondroitin sulfate E fragments enhance CD44 cleavage and CD44-dependent motility in tumor cells. Cancer Res. 68, 7191-7199.

4. Igarashi S., Osawa M., Takeuchi K., Ozawa S., Shimada I. (2008) Amino acid selective cross-saturation method for identification of proximal residue pairs in a protein-protein complex. (2008) J Am Chem Soc. 130, 12168-12176.

5. 松本昌彦、上田卓見、嶋田一夫 (2008) NMR によるソフトな分子間相

互作用解析法の開発, 生化学, 80(10),959-971.

6. 西田紀貴、嶋田一夫 (2008) コラーゲン結合タンパク質を介した生命

プロセスの活性化機構, 生化学, 80(6),483-492.

岩崎憲治 1. Iwasaki, K., Miyazaki, N., Hammar, L., Zhu, Y., Omura, T., Wu, B., Sjöborg, F., Yonekura, K., Murata, K., Namba, K., Caspar, D.L., Fujiyoshi, Y. and

Cheng, R.H. (2008). Pleomorphic Configuration of the Trimeric Capsid Proteins of Rice dwarf virus that Allows Formation of Both the Outer Capsid and Tubular Crystals. J. Mol. Biol. 383, 252-265.

2. Tanaka, H., Kato, K., Yamashita, E., Sumizawa, T., Zhou, Y., Yao, M., Iwasaki, K., Yoshimura, M. and Tsukihara, T. The structure of rat liver vault at 3.5 angstrom resolution. Science 323, 384-388.

3. 岩崎憲治 （2008）電子線トモグラフィー，生化学（ことば），80(12)，

1141．

鷹野 優 1. Takano, Y., Nakamura, H., (2008) A Theoretical Approach to a Novel Pathway of Proton Translocation of Cytochrome c Oxidase. AIP Conf. Proc. 1046, 60-63.

2. Yamasaki, H., Takano, Y, Nakamura, H.. (2008) Theoretical investigation of the electronic assymetry of the special pair cation radical in the photosynthetic type-II reaction center. J. Phys. Chem. B 112, 13923-13933.

3. Umezawa, K., Ikebe, J., Nomizu, M., Nakamura, H., Higo, J., (2009) Conformational requirement on peptides to exert laminin’s activities and search for protein segments with the laminin’s activities. Biopolymers- Peptide Science, in press.

4. 山崎秀樹、鷹野優、中村春木 (2009) 光合成反応中心スペシャルペア

の電子非対称性の構造的起源 量子化学計算とバイオインフォマティ

クスによるアプローチ, 生物物理, in press.

安永卓生 1. Ueno H., Yasunaga T., Shingyoji C., and Hirose K. (2008) Dynein pulls microtubules without rotating its stalk PNAS 105, 19702-19707

2. Liu Z., Takazaki H., Nakazawa Y., Sakato M., Yagi T., Yasunaga T., King S.M., and Kamiya R. (2008) Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain, Eukaryotic Cell, 7, 1136-1145

3. Murakami K., Stewart M., Nozawa K., Tomii K., Kudou N., Igarashi N., Shirakihara Y., Wakatsuki S., Yasunaga T., and Wakabayashi T. (2008) Structural basis for tropomyosin overlap in thin (actin) filaments and the generation of a molecular swivel by troponin-T., ProNAS, 105(20), 7200-5

4. Yasunaga, T., Wakabayashi, T. (2008) Evaluation of a 2k CCD camera with an epitaxially-grown CsI scintillator for recording energy-filtered electron cryo-micrographs., Journal of Electron Microscopy, 57(3), 101-112

5. 安永卓生 (2008)「ナノイメージング」（株）ＮＴＳ 共著 第一編ナ

ノイメージングを可能にする顕微鏡法・第３章電子顕微鏡を用いたナ

ノイメージング p.44-59

三尾和弘 1. Mio, K., Ogura, T., Yamamoto, T., Hiroaki, Y., Fujiyoshi, Y., Kubo, Y. & Sato, C. (2009) Reconstruction of the P2X2 receptor revealed a vase-shaped structure with lateral tunnels above the membrane. Structure Structure 17, 266-275

2. Omura,A., Matsuzaki,T., Mio,K., Ogura,T., Yamamoto,M., Fujita,A., Okawa,K., Kitayama,H. Takahashi,C. Sato,C. (2009) M.Noda. RECK forms cowbell-shaped dimers and inhibits MMP -catalyzed cleavage of fibronectin. J. Biol. Chem. 284(6), 3461-3469

3. Mio, K., Ogura, T., Mio, M., Shimizu, H., Hwang, T. C., Sato, C. & Sohma, Y. (2008). Three-dimensional reconstruction of human cystic fibrosis transmembrane conductance regulator chloride channel revealed an ellipsoidal structure with orifices beneath the putative transmembrane domain. J. Biol. Chem. 283, 30300-30310.

4. Mio, K., Ogura, T. & Sato, C. (2008). Structure of six-transmembrane cation channels revealed by single-particle analysis from electron microscopic images. J. Synchrotron Radiat. 15, 211-214.

5. Mio, K., Kubo, Y., Ogura, T., Yamamoto, T., Arisaka, F. & Sato, C. (2008). The motor protein prestin is a bullet-shaped molecule with inner cavities. J. Biol. Chem. 283, 1137-1145.

6. Nemoto, M., Mio, K., Kanamaru, S. & Arisaka, F. (2008). ORF334 in Vibrio phage KVP40 plays the role of gp27 in T4 phage to form a hetero-hexameric complex. J. Bacteriol. 190, 3606-3612.

7. 小椋俊彦，三尾和弘，佐藤主税（2008）電顕画像からの全自動 3 次元

再構成法の開発と TRP チャンネルの構造, 顕微鏡，43, 40-45.

A02：鞭毛、ウイルスの構造形成と感染機構の研究

大村敏博 1. Wei, T., Hibino, H. and Omura, T. Rice dwarf virus is engulfed into andreleased via vesicular compartments in cultured inset vector cells. Journal ofGeneral Virology. 89, 2915-2920 (2008).

2. Iwasaki, K., Miyazaki, N., Hammer, L., Zhu, Y., Omura, T., Wu, B., Sjoborg, F., Yonekura, K., Murata, K., Namba, K., Caspar, D. L., Fujiyoshi, Y. and Cheng,R. H. Pleomorphic configuration of the trimeric capsid proteins of Rice dwarfvirus that allows formation of both the outer capsid and tubular crystals. Journal of Molecular Biology 383, 252-265 (2008).

3. Miyazaki, N., Uehara-Ichiki, T., Xing, L., Bergman, L, Higashiura, A.,Nakagawa, A., Omura, T. and Cheng, R. H. Structural evolution of Reoviridaerevealed by Oryzavirus in acquiring the second capsid shell. Journal of Virology 82, 11344-11353 (2008).

4. Shimizu, T., Yoshii, M., Wei, T., Hirochika, H. and Omura, T. Silencing byRNAi of the gene for Pns12, a viroplasm matrix proteins of Rice dwarf virus,

results in strong resistance of transgenic rice plants to the virus. Plant Biotechnology Journal PMID: 18980655 (2008).

5. Motoyasu Yoshii, Takumi Shimizu, Muneo Yamazaki, Takahiko Higashi AkioMiyao, Hirohiko Hirochika, and Toshihiro Omura Disruption of a novel genefor a NAC-domain protein in rice confers resistance to Rice dwarf virus The Plant Journal 7, 24-32 (2009).

有坂文雄 1. Nemoto M, Mio K, Kanamaru S, Arisaka F. (2008) ORF334 in Vibrio phageKVP40 plays the role of gp27 in T4 phage to form a hetero-hexameric complex.J Bacteriol. 190(10): 3606-3612

2. Koshiyama T, Yokoi N, Ueno T, Kanamaru S, Nagano S, Shiro Y, Arisaka F,Watanabe Y. (2008) Molecular design of heteroprotein assemblies providing abionanocup as a chemical reactor.Small. Jan;4 (1):50-4.

3. 金丸周司・有坂文雄.(2007) バクテリオファージの構造生物学.「蛋白質

核酸 酵素」 Vol.52 No.10 1069-1074

4. Mio K, Kubo Y, Ogura T, Yamamoto T, Arisaka F, Sato C. (2008) The motorprotein prestin is a bullet-shaped molecule with inner cavities.J.Biol.Chem.283(2):1137-45

武田茂樹 1. Akuzawa N., Obinata H., Izumi T., Takeda S., Morphine is an exogenous ligand for MrgX2, a G protein-coupled receptor for cortistatin (2008) J. Cell Animal Biol., 2, 4-9

2. Sasaki Y., Tsujii T., Takeda S, Obinata H., Izumi T., Yamada K., Katakai R., Identification of novel peptide agonists from a random peptide library for a5-oxo-ETE receptor, a receptor for bioactive lipids (2008) J. Peptide Sci. 14, 1251-1258.

3. Tateno M., Toyooka M., Shikano Y., Takeda S, Kuwabara N., Sezutsu H., Tamura T., Production and characterization of the recombinant humanμ-opioid receptor from transgenic silkworms (2009) J. Biochem. (Tokyo), 145, 37-42

4. Toyooka M., Tsujii T., Takeda S, The N-terminal domain of GPR61, an orphan G protein-coupled receptor, is essential for its constitutive activity (2009) J. Neurosci. Res., in press.

栗栖源嗣 1. Narikawa, R., Muraki, N., Shiba, T., Ikeuchi, M., Kurisu, G. (2009) Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120. Acta Crystallogr. F65, 159-162.

2. Inaoka, D. K., Sakamoto, K., Shimizu, H., Shiba, T., Kurisu, G., Nara, T., Aoki, T., Kita, K., Harada, S. (2008) Structure of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolutioninsights into mechanisms of dihydroorotate oxidation and fumarate reduction. Biochemistry 47, 10881-10891

3. Muraki, N., Seo, D., Shiba, T., Sakurai, T., Kurisu, G. (2008) Crystallization andpreliminary X-ray studies of ferredoxin-NAD(P)+ reductase from Chlorobium tepidum, Acta Crystallogr., F64, 186-189.

4. 栗栖源嗣(2008) タンパク質の辞典, 朝倉書店, 346－350，438.

神谷 律 1. Kikushima, K. and Kamiya, R. (2008) Clockwise translocation of microtubules by flagellar inner-arm dyneins in vitro. Biophys. J. 94, 4014-4019.

2. Liu, Z., Takazaki, H., Nakazawa, Y., Sakato, M., Yagi, T.,Yasunaga, T. King, S.M., and Kamiya, R. (2008) Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated γheavy chain. Euk. Cell 7, 1136-1145.

3. Omran, H., Kobayashi, D., Olbrich, H., Tsukahara, T. Loges, N.T, Hagiwara,H., Zhang, Q., Leblond, G., O’Toole, E., Hara, H., Mizuno, H., Kawano, H., Fliegauf, H., Yagi, T., Koshida, S., Miyawaki, A., Zentgraf, H., Seithe, H.,Reinhardt, R., Watanabe, Y., Kamiya, R., Mitchell, D.R. and Takeda, H. (2008). Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature 456, 611-616.

4. Ikeda, K., Yamamoto, R., Wirschell, M., Yagi, T., Bower, R., Porter, M.E., Sale,W.S., and Kamiya, R. (2009). A Novel Ankyrin-Repeat Protein Interacts with the Regulatory Proteins of Inner Arm Dynein f (I1) of Chlamydomonas reinhardtii. Cell Motil. Cytoskeleton, in press.

5. Furuta, A, Yagi, T., Yanagisawa, H., Higuchi, H., and Kamiya, R. (2009). Systematic comparison of in vitro motile properties between Chlamydomonaswild-type and mutant outer arm dyneins each lacking one of the three heavy chains. J. Biol. Chem., in press.

6. Yagi, T., Uematsu, K., Liu, Z., and Kamiya, R. (2009). Identification of novel dyneins that localize exclusively to the proximal portion of Chlamydomonas flagella. J. Cell Sci., in press.

7. King, S. M., and Kamiya, R. (2008). Axonemal dyneins: assembly, structure and force generation. “Chlamydomonas source book, vol. III”, edited by G. B. Witman, pp. 129- 206. Elsevier Press, Amsterdam, The Netherlands.

小嶋誠司 1. Kojima, S., Shinohara, A., Terashima, H., Yakushi, T., Sakuma, M., Homma, M., Namba, K., Imada, K. (2008) Insights into the stator assembly of the Vibrioflagellar motor from the crystal structure of MotY. Proc. Natl. Acad. Sci. U. S. A. 105, 7696-7701.

2. Kojima, S., Furukawa, Y., Matsunami, H., Minamino, T., Namba, K. (2008) Characterization of the periplasmic domain of MotB and implications for itsrole in the stator assembly of the bacterial flagellar motor. J. Bacteriol. 190, 3314-3322.

3. Obara, M., Yakushi, T., Kojima, S., Homma, M. (2008) Roles of charged residues in the C-terminal region of PomA, a stator component of the

Na+-driven flagellar motor. J. Bacteriol. 190, 3565-3571. 4. Hizukuri, Y., Kojima, S., Yakushi, T., Kawagishi, I., Homma, M. (2008)

Systematic Cys mutagenesis of FlgI, the flagellar P-ring component of Escherichia coli. Microbiology 154, 810-817.

5. Terashima, H., Kojima, S., Homma,M. (2008) Flagellar motility in bacteria: structure and fnction of flagellar motor. Int. Rev. Cell Mol. Biol. 270, 39-85

A03：蛋白質複合体における高度な機能制御機構の研究

吉川信也 1. Nakamura-Ogiso, E., Matsuno-Yagi, A., Yoshikawa, S., Yagi, T., and Ohnishi, T. (2008) Iron-sulfur cluster N5 is coordinated by a HXXXCXXCXXXXXCmotife in the NuoG subunit of E. coli NADH:quinine oxidoreductase (Complex I), J. Biol. Chem. 283, 25979-25987.

2. Ohnishi, To., Ohnishi, Ts., Shinzawa-Itoh, K. and Yoshikawa, S. (2008) Functional role of Coenzyme Q in the energy coupling of NADH-CoQ oxidoreductase (Complex I): Stabilization of the semiquinone state withapplication of inside-positive membrane potential to proteoliposomes, Biofctor 32, 13-22.

3. Hiroshi Aoyama, Kazumasa Muramoto, Kyoko Shinzawa-Itoh, Kunio Hirata, Eiki Yamashita, Tomitake Tsukihara, Takashi Ogura, Shinya Yoshikawa(2009) A Peroxide Bridge between Fe and Cu Ions in the O2 Reduction Site of Fully Oxidized Cytochrome c Oxidase could suppress the Proton Pump, Proc. Natl. Acad. Sci. USA , 106, 2165-2169

神谷信夫 1. Takahiro Henmi, Masako Iwai, Masahiko Ikeuchi, Keisuke Kawakami,Jian-Ren Shen, Nobuo Kamiya (2008) X-ray crystallographic and biochemical characterizations of a mutant Photosystem II complex fromThermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation. J. Synchrotron Rad. 15, 304-307.

2. Han Bao, Chunxi Zhang, Keisuke Kawakami, Yanan Ren, Jian-Ren Shen, Jingquan Zhao (2008) Acceptor side effects on the electron transfer atcryogenic temperatures in intact photosystem II. Biochim. Biophys. Acta 1777, 1109-1115.

3. Jing Leng, Isamu Sakurai, Hajime Wada, Jian-Ren Shen (2008) Effects of phospholipase and lipase treatments on photosystem II core dimer from athermophilic cyanobacterium. Photosynthesis Research 98, 469-478.

4. Isao Enami, Akinori Okumura, Ryo Nagao, Masako Iwai, Takehiro Suzukiand Jian-Ren Shen (2008) Structures and functions of the extrinsic proteins of photosystem II from different species. Photosynthesis Research 98, 349-363.

5. Hideyuki Adachi, Yasushi Umena, Isao Enami, Takahiro Henmi, NobuoKamiya, Jian-Ren Shen (2009) Towards structural elucidation of eukaryotic photosystem II: Purification, crystallization and preliminary X-ray diffraction

analysis of photosystem II from a red alga. Biochim. Biophys. Acta 1787, 121-128.

6. Iwai, M., Suzuki, T., Dohmae, N., Inoue, Y. and Ikeuchi, M. (2007) Absenceof the PsbZ subunits prevents association of the PsbK and Ycf12 with the photosystem II in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Plant Cell Physiology 4, 1758–63.

7. Iwai, M., Maoka, T., Ikeuchi, M. and Takaichi, S. (2008) 2,2’-b-hydroxylase is involved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2’-fucoside in Thermosynechococcus elongatus strain BP-1. Plant Cell Physiology 49, 1678-1687.

8. Akinori Okumura, Ryo Nagao, Takehiro Suzuki, Satoshi Yamagoe, MasakoIwai, Katsuyoshi Nakazato, Isao Enami (2008) A novel protein in Photosystem II of a diatom Chaetoceros gracilis is one of the extrinsic proteins located on lumenal side and directly associates with PSII corecomponents Biochimica et Biophysica Acta – Bioenergetics 1777,1545-1551.

9. Akinori Okumura, Masanori Sano, Takehiro Suzuki, Hiroyasu Tanaka, RyoNagao, Katsuyoshi Nakazato, Masako Iwai, Hideyuki Adachi, Jian-Ren Shen, Isao Enami (2007) Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning FEBS Letters 581, 5255-5258.

10. Shen J.-R., Henmi T. and Kamiya N. (2008) Structure and Function ofPhotosystem II, In Photosynthetic Protein Complexes, A Structural Approach, Edited by F. Fromme, WILEY-VCH, pp. 83-106.

［産業財産権の出願・取得状況］ • Katsuyoshi Nakazato, Method of measuring protein solubility, process for

producing crystal and apparatus therefore, US 7416708B2 (2008) • 中里勝芳、「タンパク質溶解度測定方法、結晶製造方法、及びそのた

めの装置」，特許第４１１０２２４号（２００８年） 大友征宇 （大友征宇の英語名は Wang, Z.-Y.となっています）

1. Kimura, Y., Yu, L.-J., Hirano, Y., Suzuki, H., and Wang, Z.-Y.* (2009) Calcium ions are required for the enhanced thermal stability of thelight-harvesting-reaction center core complex from thermophilic purple sulfurbacterium Thermochromatium tepidum. J. Biol. Chem. 284, 93-99.

2. Kimura, Y., Hirano, Y., Yu, L.-J., Suzuki, H., Kobayashi, M. and Wang, Z.-Y.* (2008) Calcium ions are involved in the unusual red-shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purplesulfur bacterium Thermochromatium tepidum. J. Biol. Chem. 283, 13867-13873.

3. Ma, F., Kimura, Y., Zhao, X.-H., Wu, Y.-S., Wang, P., Fu, L.-M., Wang, Z.-Y.

and Zhang, J.-P. (2008) Excitation dynamics of two spectral forms of the corecomplexes from photosynthetic bacterium Thermochromatium tepidum. Biophys. J., 95, 3349-3357.

永田宏次 1. Imai, F. L., Nagata, K., Yonezawa, N., Nakano, M. and Tanokura, M. (2008). Crystal structure of calcium-bound human S100A13 at pH 7.5 at 1.8-Å resolution. Acta Crystallogr. F64, 70-76

2. Yamamura, A., Ichimura, T., Mimoto, F., Ohtsuka, J., Miyazono, K., Okai, M., Kamo, M., Lee, W.-C., Nagata, K. and Tanokura, M. (2007). A unique catalytic triad revealed by the crystal structure of APE0912, a short-chain dehydrogenase/reductase family protein from Aeropyrum pernix K1. Proteins70, 1640-1645.

3. Okai, M., Miyauchi, Y., Lee, W. C., Yumoto, F., Nagata, K. and Tanokura, M. (2007). Crystal structure of the proline iminopeptidase-related protein TTHA1809 from Thermus thermophilus HB8. Proteins 70, 1646-1649.

4. Yumoto, F., Tanaka, H., Nagata, K., Miyauchi, Y., Miyakawa, T., Ojima, T. and Tanokura, M. (2008). Spectroscopic and ITC study of the conformationalchange upon Ca2+-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle. Biochem. Biophys. Res. Commun. 369, 109-114.

5. Yamamura, A., Ohtsuka, J., Kubota, K., Agari, Y., Ebihara, A., Nakagawa, N.,Nagata, K. and Tanokura, M. (2008). Crystal structure of TTHA1429, a novelmetallo-β-lactamase superfamily protein from Thermus thermophilus HB8. Proteins 73, 1053-1057.

6. Nishiguchi, K., Nagata, K., Tanokura, M., Sonomoto, K. and Nakayama, J. (2009). Structure-activity relationship of gelatinase biosynthesis-activating pheromone of Enterococcus faecalis. J. Bacteriol. 191, 641-650.

福森義宏 1. N.Numoto, T.Nakagawa, A.Kita, Y.Sasayama, Y.Fukumori and K.Miki Structural Basis for the Heterotropic and Homotropic Interactions ofInvertebrate Giant Hemoglobin. Biochemistry, 47, 11231–11238, 2008

2. Numoto N, Nakagawa T, Kita A, Sasayama Y, Fukumori Y, Miki K, Structure of the partially unliganded met state of 400 kDa hemoglobin: insights into ligand-induced structural changes of giant hemoglobins. Proteins. 73(1), 113-125, 2008

3. Taoka A, Umeyama C, and Fukumori Y Identification of iron transporters expressed in the magnetotactic bacterium Magnetospirillum magnetotacticum. Curr. Microbiol.,58, 177-181, 2009

4. 沼本修孝，中川太郎，福森義宏，三木邦夫 巨大ヘモグロビンの構造と

機能. 日本血液代替物学会会誌「人工血液 Artificial Blood」印刷

中

5. 福森義宏 特集：原核細胞の細胞骨格「特集に当たって」 蛋白質 核

酸 酵素 vol.53 No.13，p1719 - p1722, 2008

6. 福森義宏、 田岡東 磁性細菌のオルガネラ、マグネトソームの細胞内

局在と細胞骨格 蛋白質 核酸 酵素 vol.53 No.13，p1746 - p1751,

2008

石森浩一郎 1. Kitanishi, K., Igarashi, J., Hayasaka, K., Hikage, N., Saiful, I., Yamauchi, S.,Uchida, T., Ishimori, K., Shimizu, T. (2008) Heme-binding characteristics of the isolated PAS-A domain of mouse Per2, a transcriptional regulatory factorassociated with circadian rhythms. Biochemistry, 47, 6157-6168.

2. Uzawa, T., Nishimura, C., Akiyama, S., Ishimori, K., Takahashi, S., Dyson, H. J., Wright, P. E. (2008) Hierarchical Folding Mechanism of ApomyoglobinRevealed by Ultra-fast H/D Exchange Coupled with 2D NMR. Proc. Natl. Acad. Sci. USA, 105, 13859-13864.

Boero Mauro

1. Boero, M., Kang, J., Tokumoto, S., and Tateno, M. (2009) A First-Principle Exploration of Heme a and Heme a3 of the Bovine Cytochrome c Oxidase in Reduced and Oxidized Charge States. J. Comp. Theor. Nanosci., in press.

2. Scipioni,R., Schmidt, D. A., and Boero, M (2009) A First Principles Investigation of Water Dipole Moment in a Defective Continuous Hydrogen Bond Network. J. Chem. Phys. 130, 024502.

3. Kamiya, K., Yamamoto, S., Shiraishi, K., and Oshiyama, A. (2009) A Significant Change in Electronic Structures of Heme Upon Reduction by Strong Coulomb Repulsion between Fe d Electrons. J. Phys. Chem. B, in press.

4. Hagiwara, Y. and Tateno, M. (2009) QM/MM hybrid calculation of biological macromolecules using new interface program connecting QM and MM engines. J. Phys. : Condens. Matter, 21, 064224.

5. Kang, J., Ohta, T., Hagiwara, Y., Nishikawa, K., Yamamoto, T., Nagao, H., and Tateno, M. (2009) Electronic and geometric structures of the blue copper site of azurin investigated by QM/MM hybrid calculations. J. Phys. : Cond. Mat., 21, 064235.

6. Tateno, M. and Hagiwara, Y. (2009) Evaluation of stabilization energies in π–π and cation–π interactions involved in biological macromolecules by ab initio calculations: J. Phys. : Cond. Mat., 21, 064243.

7. Ohta, T., Hagiwara, Y., Kang, J., Nishikawa, K., Yamamoto, T., Nagao, H., and Tateno, M. (2009) Evaluation of Electronic and Geometrical Properties of the Blue Copper Site in Fully Solvated Azurin by QM/MM Hybrid Calculations Using a New Interface Program Connecting QM and MM Engine. J. Comp. Theor. Nanosci., in press.

8. Hagiwara, Y., Nureki, O., and Tateno, M. (2009) Structural modelling of the complex of leucyl-tRNA synthetase and mis-aminoacylated tRNALeu. FEBS Letters, 583, 825.

9. 舘野 賢，ボエロ マウロ (2008) 第一原理計算が明らかにする生体

反応の精巧な仕組み, 生物物理（日本生物物理学会）, 48, 216-220

(2008).

［新聞報道］

• 日経産業（全国版） 2009 年 1 月 21 日

• 日経 BP（オンライン版） 2009 年 1 月 26 日

• 茨城新聞 2009 年 1 月 21 日

深井周也 1. Tsukazaki, T., Mori, H., Fukai, S., Ishitani, R., Mori, T., Dohmae, N., Perederina, A., Sugita, Y., Vassylyev, D. G., Ito, K., Nureki, O. (2008) Conformational transition of Sec machinery inferred from bacterial SecYEstructures. Nature, 455, 988-991

2. Sato, Y., Yoshikawa, A., Yamagata, A., Mimura, H., Yamashita, M., Ookata, K., Nureki, O., Iwai, K., Komada, M., Fukai, S. (2008) Structural basis for specific cleavage of Lys 63-linked polyubiquitin chains. Nature, 455, 358-362

3. 深井 周也、佐藤 裕介、吉川 梓 (2009) Lys63 結合型ポリユビキ

チン鎖の選択的切断の構造的基盤, 実験医学，27(3),411-414.

［新聞報道］

• 毎日新聞（東京朝刊）、2008 年 9 月 7 日

• 日経産業新聞（朝刊）、2008 年 9 月 1 日

• 日経バイオ、2008 年 9 月 1日

水島恒裕 1. Crystal structure of a chaperone complex that contributes to the assembly ofyeast 20S proteasomes, Yashiroda H, Mizushima T, Okamoto K , Kameyama T, Hayashi H, Kishimoto T, Niwa S, Kasahara M, Kurimoto E, Sakata E,Takagi K, Suzuki A, Hirano Y, Murata S, Kato K, Yamane T , Tanaka K, Nat Struct Mol Biol. 15, 228-236. (2008)

2. Structure of D-amino-acid amidase complexed with L-phenylalanine with L-phenylalanine amide: insight into D-stereospecificity of D-amino-acid amidase from Ochrobacterum anthropi SV3, Okazaki S, Suzuki A, Mizushima T, Komeda H, Asano Y, Yamane T, Acta Cryst. D64, 331-334.(2008)

3. Structural basis for sorting mechanism of p62 in selective autophagy IchimuraY, Kumanomidou T, Sou Y, Mizushima, T, Ezaki, J, Ueno T, Kominami E, Yamane T, Tanaka K, Komatsu M, J. Biol. Chem 283, 22847-22857. (2008)

4. 出芽酵母20Sプロテアソーム形成に関わるシャペロン複合体Dmp1/Dmp2

の立体構造解析 八代田秀樹、水島恒裕, 細胞工学, 秀潤社 27,

474-475. (2008)

北所健悟 1. Takao, M., Oohata, Y., Kitadokoro, K., Kobayashi, K., Iwai, S., Yasui, A., Yonei, S., Zhang, Q. M. (2009) Human Nei-like protein NEIL3 has AP lyase activity specific for single-stranded DNA and confers oxidative stress resistance in Escherichia coli mutant. Genes Cells. 14, 261-270.

2. 北所健悟，神谷重樹，堀口安彦 (2008) Pasteurella multocida由来の

細菌毒素の細胞内機能領域の構造と機能解析, 日本結晶学会誌，

50(3), 187-194.

林 高史 1. A Role of the Heme-7-Propionate Side Chain in Cytochrome P450cam as a Gate for Regulating the Access of Water Molecules to the Substrate-Binding Site (2009) Takashi Hayashi, Katsuyoshi Harada, Keisuke Sakurai, HideoShimada, Shun Hirota J. Am. Chem. Soc., 131(4), 1398–1400.

2. Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme–Heme Pocket Interactions (2009) Hiroaki Kitagishi, Yasuaki Kakikura, Hiroyasu Yamaguchi, Koji Oohora, AkiraHarada, Takashi Hayashi Angew. Chem. Int. Ed., 48(7), 1271–1274.

3. Thermodynamically Controlled Supramolecular Polymerization ofCytochrome b562 (2009) Hiroaki Kitagishi, Koji Oohora, TakashiHayashi Takashi Biopolymers, 91(3), 194–200.

廣田 俊 1. Harada, K., Sakurai, K., Ikemura, K., Ogura, T., Hirota, S., Shimada, H., Hayashi, T.* (2008) Evaluation of the functional role of the heme-6-propionate side chain in cytochrome P450cam. J. Am. Chem. Soc.130, 432-433.

2. Fujii, T., Yamaguchi, S., Hirota, S., Masuda, H.* (2008) H-atom abstraction reaction for organic substrates via mononuclear copper(II)-superoxo species as a model for DbM and PHM. Dalton Trans. 164-170.

3. Noguchi, M., Skwarczynski, M., Prakash, H., Hirota, S., Kimura, T., Hayashi, Y., Kiso, Y.* (2008) Development of novel water-soluble photocleavable protective group and its application for design of photoresponsive paclitaxel rodrugs. Bioorg. Med. Chem. 16, 5389-5397.

4. Yoshidome, T., Kinoshita, M., Jr.*, Hirota, S., Baden, N., Terazima, M. (2008) Thermodynamics of apoplastocyanin folding: comparison betweenexperimental and theoretical results. J. Chem. Phys. 128, 225104 (9 pages).

5. Prakash, H., Shodai, A., Yasui, H., Sakurai, H., Hirota, S.* (2008) Photocontrol of spatial orientation and DNA cleavage activity ofcopper(II)-bound dipeptides linked by an azobenzene derivative. Inorg. Chem.47, 5045-5047.

6. Kano, K.*, Itoh, Y., Kitagishi, H., Hayashi, T., Hirota, S. (2008) A Supramolecular receptor of diatomic molecules (O2, CO, NO) in aqueous solution. J. Am. Chem. Soc. 130, 8006-8015.

7. Zafer, U.*, Satake, A., Kobuke, Y.*, Hirota, S.* (2008) Stable supramolecular complex of porphyrin macroring with pyridyl and fullerenyl ligands. Tetrahedron Lett. 49, 5484-5487.

8. Kajita, Y., Matsumoto, J., Takahashi, I., Hirota, S., Funahashi, Y., Ozawa, T., Masuda, H.* (2008) Syntheses, characterization and reactivities of

μ-η :η -disulfidodicopper(II) complexes with N-alkylated cis,cis-1,3,5-triamionocyclohexane derivatives. Eur. J. Inorg. Chem.3977-3986.

2 2

9. Sugimoto, H.*, Suyama, K., Sugimoto, K., Miyake, H., Takahashi, I., Hirota, S., Itoh, S. (2008) A new class of sulfido/oxo(dithiolene)–molybdenum(IV) complexes derived from sulfido/oxo–bis(tetrasulfido)molydebnum(IV) anions. Inorg. Chem. 47, 10150-10157.

10. Hirota, S.*, Kawahara, T., Beltramini, M., Di Muro, P., Magliozzo, R. S.,Peisach, J., Powers, L. S., Tanaka, N., Nagao, S., Bubacco, L.* (2008) Molecular basis of the Bohr effect in arthropod hemocyanin. J. Biol. Chem.283, 31941-31948.

11. Fujisawa, K., Satake, A.*, Hirota, S., Kobuke, Y.* (2008) Construction of giant porphyrin macrorings self-assembled from thiophenylene-linked bisporphyrins for light-harvesting antenna. Chem. Eur. J. 14, 10735-10744.

12. Funahashi, Y.*, Nishikawa, T., Wasada-Tsutsui, Y., Kajita, Y., Yamaguchi, S.,Arii, H., Ozawa, T., Jitsukawa, K., Tosha, T., Hirota, S., Kitagawa, T., & Masuda, H.* (2008) Formation of a bridged butterfly-type μ-η2:η2-peroxo dicopper core structure with a carboxylate group. J. Am. Chem. Soc. 130, 16444-16445.

13. Taniguchi, A., Skwarczynski, M., Sohma, Y., Okada, T., Ikeda, K., Prakash,H., Mukai, H., Hayashi, Y., Kimura, T., Hirota, S., Matsuzaki, K., Kiso, Y.*(2008) Water-Soluble "Click Peptide" using O-acyl isopeptide method: controlled production of Alzheimer's amyloid beta peptide fromphoto-triggered precursor peptide. ChemBioChem 9, 3055-3065.

14. Hayashi, T.*, Harada, K., Sakurai, K., Shimada, H.*, Hirota, S. (2009)Functional role of heme-7-propionate side chain in cytochrome P450cam: a “gate” regulating the access of water molecules to the substrate-binding site. J. Am. Chem. Soc. 131, 1398-1400.

箱嶋敏雄 1. Takai, Y., Kitano, K., Terawaki, S., Maesaki, R., Hakoshima, T. (2008) Structural basis of the cytoplasmic tail of adhesion molecule CD43 and itsbinding to ERM proteins. J. Mol. Biol. 381, 634-644.

2. Terawaki, S., Kitano, K., Aoyama, M., Hakoshima, T. (2008) Crystallographic characterization of the radixin FERM domain bound to the cytoplasmic tail ofmembrane-type 1 matrix metalloproteinase (MT1-MMP), Acta Crystallogr.F64, 911-913

3. Mori, T., Kitano, K., Fukami, Y., Terawaki, S., Hakoshima, T. (2008) Structural basis for CD44 recognition by ERM proteins. J. Biol. Chem. 283, 29602-29612.

4. Gohda, K., Hakoshima, T. (2008) A molecular mechanism of P-loop pliability of Rho-kinase investigated by molecular dynamic simulation. J. Comput.

Aided Mol. Des. 22, 789-797. 5. Terawaki, S., Kitano, K., Hakoshima, T. (2008) Crystallographic

characterization of the membrane-targeting domain of Rac-specific guanine nucleotide exchange factors Tiam1 and 2. Acta Crystallogr. F64, 1039-1042

6. Murase, K., Hirano, Y., Sun, T.-p. Hakoshima, T. (2008) Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature 456, 459-464.

7. 箱嶋敏雄、村瀬浩司、平野良憲 (2009) ジベレリン受容体の構造と機

能の解明：植物ホルモン識別と標的タンパク質認識の機構、細胞工学、

28, 秀潤社、162-163.

8. 合田圭吾、箱嶋敏雄 (2009). 構造解析に基づく Rho キナーゼ阻害剤の

開発、実験医学 2009 年 3 月増刊号「分子標的薬開発への挑戦」、羊土

社、印刷中

［新聞報道等］

国内

• 朝日新聞朝刊 2008.11.27 植物ホルモン仕組み解明／作物収穫増

にも応用：奈良先端大など

• 毎日新聞朝刊 2008.11.27 植物ホルモン「ジベレリン」／細胞内

の働きを三次元化 世界初 自然災害に強い農作物開発に期待：奈

良先端大

• 読売新聞朝刊 2008.11.27 植物ホルモンメカニズムを解明

• 日経産業新聞朝刊 2008.11.27 成長ホルモン／植物の機構解明：

奈良先端大

• 産経新聞朝刊 2008.11.27 風雨に強い農作物を育てるホルモン

• 日刊工業新聞朝刊 2008.11.27 細胞内での動き解析／奈良先端大

／改変の設計容易に

• 奈良新聞朝刊 2008.11.27 農産物量産へ科学の力／先端大「ジベ

レリン」の働き解明：植物の成長調節／風害対応品種も

• 奈良日日新聞朝刊 2008.11.28 災害に強い稲／食糧増産に夢／先

端科技大の箱嶋教授ら：植物ホルモン「ジベレリン」３次元で作用

機構解明

• フジサンケイビジネスアイ朝刊 2008.12.04 植物ホルモンの作用

方法を解明

• 朝日新聞朝刊 2008.12.08 発芽や開花を即すホルモン／細胞内で

働く仕組み解明：奈良先端科技大など

• 科学新聞 2008.12.12 ジベレリンの働き／分子レベルで立体表

現：奈良先端大の研究グループ成功

海外 • Hedden, P. (2008.11.27). NEWS & VIEWS: PLANT BIOLOGY

Gibberellins close the lid. Nature 256 (7221), 455-456. • Everts, S. (2008.12.01). Plant puberty. C&EN (Chemical and

Engineering News) 86 (48), 9. • Ritter, S., Becher, N. and Blair, T. (2008.12.22). Chemical Year in Review

2008 Gibberellin’s role in Plant puberty. C&EN (Chemical and Engineering News) 86 (51), 14.

• Sheppard, T. L. (2009.01.22). RESEARCH HIGHLIGHTS Gibberellinlockdown. Nature Chemical Biology 5 (2), 80.

池水信二 1. Mukai, Y., Shibata, H., Nakamura, T., Yoshioka, Y., Abe, Y., Nomura, T.,Taniai, M., Ohta, T., Ikemizu, S., Nakagawa, S., Tsunoda, S., Kamada, H.,Yamagata, Y., Tsutsumi, Y. (2009) “Structure-Function Relationship of Tumor Necrosis Factor (TNF) and Its Receptor Interaction Based on 3D Structural Analysis of a Fully Active TNFR1-Selective TNF Mutant.” J. Mol. Biol. 285, 1221-1229, 2009

2. Kim, J., Motomiya, Y., Ueda, M, Nakamura, M., Misumi, Y., Saito, S.,Ikemizu, S., Misumi, S., Ota, K., Shinriki, S., Kai, H., & Ando, Y. (2008) “Role of the conformational change in the C-terminus of b2-microglobulin in dialysis-related amyloidosis” Ann. Clin. Biochem. 45, 489-495

3. Suwa, Y., Nakamura, T., Toma, S., Ikemizu, S.,Kai, H., and Yamagata (2008)“Preparation, crystallization and preliminary X-ray diffraction analysis of the DNA-binding domain of the Ets transcription factor in complex with targetDNA” Acta Crysatllogr. F64, 171-174

4. 池水 信二， “IL-2 と IL-15 の・鎖への結合様式” 臨床免疫・アレ

ルギー科 第 50 巻, 611-617, 2008

佐藤 衛 1. Akaboshi, M., Hashimoto, H., Ishida, H., Saijo, S., Koizumi, N., Sato, M., Shimizu, T. (2008) The Crystal Structure of Plant-Specific Calcium-Binding Protein AtCBL2 in Complex with the Regulatory Domain of AtCIPK14. J. Mol. Biol. 377, 246-257.

2. Hishiki, A., Shimizu, T., Serizawa, A., Ohmori, H., Sato, M., Hashimoto, H. (2008) Crystallographic study of G178S mutant of human proliferating cellnuclear antigen. Acta Crystallogr. F64, 819-821.

3. Oda, T., Hashimoto, H., Kuwabara, N., Hayashi, K., Kojima, C., Kawasaki,T., Shimamoto, K., Sato, M., Shimizu, T. (2008) Crystallographic characterization of the N-terminal domain of a plant NADPH oxidase. Acta Crystallogr. F64, 867-869.

4. Hishiki, A., Shimizu, T., Hanafusa, T., Ohmori, H., Sato, M., Hashimoto, H. (2008) Initial crystallographic study of human PCNA in complex with apeptide containing the non-canonical PIP-box sequence of human DNA polymerase ι. Acta Crystallogr. F64, 954-956.

5. Yamane, T., Okamura, H., Ikeguchi, M., Nishimura, Y., Kidera, A. (2008) Water-mediated interactions between DNA and PhoBDNA-binding/transactivation domain: NMR-restrained molecular dynamics in

explicit water environment. Proteins, 71, 1970-1983 (2008) 6. Sekiyama, N., Ikegami, T., Yamane, T., Ikeguchi, M., Uchimura, Y., Baba,

D., Ariyoshi, M., Tochio, Saitoh, H., Shirakawa, M. (2008) Structure of theSUMO-interacting motif of MCAF1 bound to SUMO-3. J. Biol. Chem., 283, 35966-35975.

7. Oroguchi, T., Hashimoto, H., Shimizu, T., Sato, M., Ikeguchi, M., Intrinsic dynamics of restriction endonuclease EcoO109I studied by moleculardynamics simulations and X-Ray scattering data analysis. Biophys. J., in press.

8. 清水敏之、佐藤 衛 (2008) タンパク質の核内輸送機構の構造的基盤,

生化学, 80, 493-500.

9. 池口満徳 (2008) 水を輸送する分子マシン アクアポリンの分子シミュ

レーション, 新分子マシン ナノで働く“高度な機械”を目指して,

化学同人編集部 編, 化学同人 132-137.

10. 木寺詔紀, 池口満徳 (2008) アクアポリンの水透過過程の比較分子シ

ミュレーション, 生化学, 80, 940-947.

11. M. Ikeguchi (2009) Water transport in aquaporins: molecular dynamics simulations, Frontiers in Bioscience, 14, 1283-1291.

菓子野康浩 1. Aikawa S, Hattori H, Gomi Y, Watanabe K, Kudoh S, Kashino Y & Satoh K (2008) Diel tuning of photosynthetic systems in ice algae at Saroma-ko Lagoon, Hokkaido, Japan. Polar Sci: in press.

2. Inoue-Kashino N, Takahashi T, Ban A, Sugiura M, Takahashi Y, Satoh K &Kashino Y (2008) Evidence for a stable association of Psb30 (Ycf12) withphotosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803. Photosynth Res 98: 323-335.

3. Islam MR, Aikawa S, Midorikawa T, Kashino Y, Satoh K & Koike H (2008) slr1923 of Synechocystis sp. PCC6803 is essential for conversion of 3,8-divinyl(proto)chlorophyll(ide) to 3-monovinyl(proto)chlorophyll(ide). Plant Physiol 148: 1068-1081.

4. Ikeda Y, Komura M, Watanabe M, Minami C, Koike H, Itoh S, Kashino Y & Satoh K (2008) Photosystem I complexes associated withfucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis. Biochim Biophys Acta 1777: 351-361.

島田秀夫 1. Ikemura, K., Mukai, M., Shimada, H., Tsukihara, T., Yamaguchi, S.,Shinzawa-Itoh, K., Yoshikawa, S., and Ogura, T., (2008) Red-ExcitationResonance Raman Analysis of the νFe=O Mode of Ferryl-Oxo Hemoprotein, J. Am. Chem. Soc. 130, 14384-14385.

2. Hayashi, T., Harada, K., Sakurai, K., Shimada, H., and Hirota, S. (2009) ARole of the Heme-7-Propionate Side Chain in Cytochrome P450cam as a Gatefor Regulating the Access of Water Molecules to the Substrate-Binding Site, J.

Am. Chem. Soc. 131, 1398-1400. 3. Sakurai, K., Shimada, H., Hayashi, T., and Tsukihara, T., (2009) Substrate

binding induces structural changes in cytochrome P450cam, Acta. Cryst. F65, 80-83.

若林健之 1. K. Murakami, M. Stewart, K. Nozawa, K. Tomii, N. Kudou, N. Igarashi, Y.Shirakihara, S. Wakatsuki, T. Yasunaga, & T. Wakabayashi (2008): Structural basis for tropomyosin overlap in thin (actin) filaments and the generation of amolecular swivel by troponin-T. Proc. Natn. Acad. Sci. U. S. A., 105, 7200-7205.

2. T. Yasunaga, & T. Wakabayashi (2008): Evaluation of a 2k CCD camera with an epitaxially-grown CsI scintillator for recording energy-filtered electron cryo-micrographs. J. Electron Microsc. (Tokyo), 57, 101-112.

［産業財産権の出願・取得状況］

• ＯＣＴの両端余弦窓関数による像の改善 特願2007-196418（発明者）

（申請中） 大林 康二，若林 健之

川岸郁朗 1. Hizukuri, Y., Kojima, S., Yakushi, T., Kawagishi, I., and Homma, M.:Systematic Cys mutagenesis of FlgI, theflagellar P-ring component of Escherichia coli. Microbiology 154: 810-817 (2008)

2. 坂野聡美，本間道夫，川岸郁朗：蛋白質の細胞内局在と細胞骨格． 蛋

白質核酸酵素 53: 1739-1745 (2008)

3. 鈴木大介，西山宗一郎，川岸郁朗：私達の研究：細菌のもつ化学セン

サーのしくみを探る． 化学療法の領域 24: 1092-1099 (2008)

4. 塩見大輔，川岸郁朗：細菌の膜貫通型受容体の局在とそのメカニズム．

生物物理 48: 30-34 (2008)

5. 川岸郁朗： 第 3章 8 べん毛と走化性． 『医科細菌学』改訂第４版（笹

川千尋，林哲也 編） 南江堂 pp.87-96 (2008)

永野真吾 1. Yong Wang, Hajime Hirao, Hui Chen, Hiroyasu Onaka, Shingo Nagano and *Sason Shaik (2008) Electron Transfer Activation of Chromopyrrolic Acid byCytochrome P450 En Route to the Formation of an AntitumorIndolocarbazole Derivative: Theory Supports Experiment. J. Am. Chem. Soc. 130, 7170-7171.

富田耕造 1. Toh, Y., Numata, T., Watanabe, K., Takeshita, D., Nureki, O., Tomita, K. (2008) Molecular basis for maintenance of fidelity during the CCA-adding reaction by a CCA-adding enzyme. EMBO J., 27, 1944-1952.

2. 董雪松、 富田耕造 (2008) 鋳型非依存的RNA合成酵素，CCA付加酵素の

RNA合成忠実性維持の分子基盤 実験医学, 26(19)、3058-3061羊土社出

版

3. 渡邉和則、 富田耕造 (2008) tRNA を使うリボソームによらないペプチ

ド結合形成反応 蛋白質核酸酵素、53(9)、1152-1157. 共立出版

［新聞報道］

• プレスリリース「鋳型なしRNA合成において正しい配列を維持するため

の 分 子 機 構 を 解 明 」 2008 年 7 月 8 日

http://www.aist.go.jp/aist_j/press_release/pr2008/pr20080708/pr20080708.html • 日刊工業新聞 2008 年 7 月 9 日（水）

• 日経産業新聞 2008 年 7 月 9 日（水）

• 科学新聞 2008 年 7 月 18 日（金）

千田俊哉 1. Senda, M., Muto, S., Horikoshi, M., Senda, T. (2008) Effect of leucine-to-methionine substitutions on the diffraction quality of histonechaperone SET/TAF-Ibeta/INHAT crystals. Acta Crystallogr. Sect. F 64, 960-965.

2. Adachi, N., Senda, M., Natsume, R., Senda, T., Horikoshi, M. (2008) Crystal structure of Methanococcus jannaschii TATA box-binding protein. Genes Cells 13, 1127-1140.

3. Eitoku, M., Sato, L., Senda, T., Horikoshi, M. (2008) Histone chaperones: 30 years from isolation to elucidation of the mechanisms of nucleosomeassembly and disassembly. Cell Mol. Life Sci. 65, 414-444.

4. 夏目亮、栄徳勝光、赤井祐介、佐野徳彦、堀越正美、千田俊哉 (2008)

ヒストンシャペロン CIA によるヌクレオソーム構造変換とエピジェネ

ティクス, 生物物理, 48, 108-113.

5. 千田美紀、木村成伸、福田雅夫、石田哲夫、千田俊哉 (2008) 反応中

間体構造から明らかになった電子伝達タンパク質間の酸化還元依存的

な親和性調節機構, 日本結晶学会誌, 50, 341-347.

6. 千田美紀、木村成伸、福田雅夫、石田哲夫、千田俊哉 (2008) 電子伝

達タンパク質間の酸化還元状態依存的親和性調節機構, 化学と生物,

46, 689-696.