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Objective SWBAT identify the process of transcription as it relates to The Central Dogma by accurately transcribing 13/15 DNA sequences into mRNA

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Central Dogma of molecular biology

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Central Dogma of molecular biology Main or important Belief or principle Main Belief The flow of genetic information in cells is from __DNA___ to __RNA__ to __protein______. It involves 2 steps:transcription &translation

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Transcription DNARNAPROTEIN Central Dogma of molecular biology Central Dogma part 1

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DNA RNA ribosomes endoplasmic reticulum vesicle Golgi apparatus vesicle protein on its way! protein finished protein Making Proteins TO: nucleus

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Key Players KEY PLAYERS DNARNARibosomes LocationNucleusNucleus & Cytoplasm Cytoplasm

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What is Transcription? Comes from the word _________________ To transcribe something means to _______ it in another way. transcribe copy transcription In love Knockin on heavens door Like a rolling stone All along the water tower

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Why is Transcription Important? Why do we need to make a new copy? Help, I cant get out! Ribosome DNA cannot Leave the __________. How can a message get to the ribosome? ___________ mRNA Nucleus m = Messenger

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Transcription: making a copy

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Step 1: RNA Helicase ________ DNA unwinds TATA TATA AGCTCGAACGGA TCCGTTCGAGCT A G C T C G AA C G G A T C C GTTCG A G C T 3 5 3 RNA Helicase

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Transcription: making a copy Step 2: RNA Polymerase ______nucleotides to mRNA in the 5 3 direction adds TATA TATA A G C T C G AA C G G A T C C GTTCG A G C T 3 5 3 UCCGUUCGAGC RNA polymerase mRNA Exception to base-pair rule: ___ instead of ___ UT

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Practice: What is the complementary DNA strand? 1.Template: 5 TACT 3 1.Template: 3 GCAT 5

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*Step 2A: There are useless DNA codes called INTRONS that get spliced out. The pieces that are left, called EXONS, form the actual mRNA. Pre-mRNA: UCCGGAGUAAC AGUGGA intron exon GAGUAA mRNA: UCCGC Exons are expressed! DNA RNA = Transcription

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Transcription: making a copy Step 3: DNA _________ to double helix and mRNA ________ the nucleus returns TATA TATA A G C T C G AA C G G A 3 5 3 UCCGUUCGAGC mRNA leaves T C C GTTCG A G C T AGCTCGAACGGA TCCGTTCGAGCT

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DNA RNA ribosomes endoplasmic reticulum vesicle Golgi apparatus vesicle protein on its way! protein finished protein Making Proteins TO: nucleus

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Video: Transcription Summary

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PRACTICE DNA Strand 1: ATA CGA CAT AGG AAC TAT CAG mRNA Strand: DNA Strand 2: AAA GGG CCC GAT AGA ACC GAA mRNA Strand: DNA Strand 3: GGG CCC GAT AGG GAA AAT GAG mRNA Strand: DNA Strand 4: GGA TAC GAT CAT ATA CCA ATC mRNA Strand: