Numira core-presentation-012414

Kim Killian

Regional Manager So Cal

February 21, 2014

Oncology

Services

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About Us

SPECIALTY CRO that combines novel tools & technologies to provide unique solutions to a wide variety of preclinical drug discovery efforts

RECOGNIZED LEADER for high-resolution 3D imaging and analysis, specialized histology, and

3D cell screening oncology services

High-quality portfolio of services for broad range of Rx areas, including: PULMONARY,

ONCOLOGY, INFLAMMATION, BONE, DEVELOPMENTAL BIOLOGY, DEVICES

Helping the Life Sciences community develop better products through

the most innovative tools and technologies

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MicroCT Imaging and Analysis

RA Rat Paw

AltaBlu-perfused Rat Brain

Surface Roughness

Fibrotic Mouse Lungs

Vessel Radius

Segmented Fibrotic Tissue

UNIQUE

Image Analysis and Quantification


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Pulmonary ServicesBleomycin-induced Lung Fibrosis

The Problem

The fibrotic lesions generated by bleomycin are

heterogeneous and vary depending on the route of

induction.

The heterogeneous nature of the lesion makes it

challenging at best to reconcile hydroxyproline

measures with the histological measures, particularly

with the intra-tracheal administration model.

Our unique solution

Using a patented contrast agent and high-resolution

microCT imaging, we obtain images that allow you to

visualize all of the fibrosis in the entire lung at a

glance.

The fibrosis is quantified and graphed for

statistical analysis for the entire lung.

The imaging is non-destructive. The

comprehensive nature of imaging data allows a

focused approach to histology sampling or

hydroxyproline measures of collagen.

Control (normal) and Fibrotic (bleomycin-induced)

lungs showing fibrotic lesions


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Pulmonary ServicesBleomycin-induced Lung Fibrosis – MicroCT Results

3 normal and 3 (bleomycin-induced) fibrotic lungs scanned at 10μm resolution using MicroCT

Subcutaneous implantation of micro-pump calibrated to deliver measured dosages with an

exposure duration of 7 days

Pump removed and specimens allowed to ambulate freely for 5 weeks after administration of

reagent

Specimen lungs were perfused to facilitate blood clearance

Sample No.

Hyper-attenuating

Tissue Volume

(mm3)

Total Tissue

Volume (mm3)

Ratio

(HTV/TTV)

52 (Normal) 1.682 242.493 0.69%

153 (Normal) 2.292 220.171 1.04%

154 (Normal) 3.927 210.557 1.87%

353 (Fibrotic) 9.708 187.963 5.16%

354 (Fibrotic) 36.878 212.730 17.34%

454 (Fibrotic) 13.328 219.508 6.07%

Fibrotic

Normal


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Pulmonary ServicesBleomycin-induced Lung Fibrosis

Hyper-attenuating tissue

highlighted in yellowTraditional Histology MicroCT Imaging Data

Comparison between traditional histology and microCT data for a single coronal section


Pulmonary Services

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Fibrosis InflammationNormal

Comparison – Lungs with fibrotic, normal, and inflammatory tissue

Emphysema / COPD

MicroCT Imaging and

3D Analysis

Pulmonary Arterial

Hypertension

Vascular analysis

using AltaBluTM

Analysis using

Immunohistochemistry

(IHC)

Additional Services


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Vasculature Services

Kidney

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AltaBluTM – Vascular Casting Agent

Comprehensive visualization of vasculature features (even within bone)

Can be used with Histology & IHC

Easy to use; no mixing; comes pre-formulated

Easy to clean up (with water)

More successful perfusions

More consistently penetrate capillaries8

AltaBlu PrimeTM – Vascular Casting Agent


Histology ServicesFull Service GLP Histology Suite

Routine Histology

Paraffin, Frozen, and Plastic

Specialized Histology

Thin sections and EXAKT ground sections for hard

biomaterials including metals

Oversized sections

Specialized expertise in ocular and brain

Histomorphometry

GLP-validated Osteomeasure systems

Static, dynamic, and cellular measurements

Histopathology

Bone, Oncology, CNS, Pulmonary, Gastrointestinal, and

Renal

Special Stains

Several stains including Goldner’s, TRAP, Masson’s, and

Silver Stain

Immunohistochemistry

Method development

Co-expression and double labeling

Image Analysis

Custom analysis tools and segmentation available

Comparison between traditional histology and microCT data for a single coronal section.

Post soft tissue imaging, samples can further be used for IHC or traditional histology.

Traditional Histology MicroCT Imaging Data

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Histology ServicesImage Analysis

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Custom segmentation and image analysis

using in-house software tools (VHLab)

Color-based analysis for different features of

interest

Area/Size calculations for specific stains or

features

Stain color distribution

Cell count

Study-dependent custom metrics

No. of glomeruli analyzed: 6

Glomerular tuft area (blue regions): 57051 sq. microns

Brown stained area (red regions): 28297 sq. microns

Average radius: 43.57 ± 10.76 microns

Glepp-1 stained Normal Rat Kidney


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Software ServicesAltaNexusTM – Cloud-based Service

https://AltaNexus.com

Security: authenticated logins, encrypted storage, and secure web-connections

Data access: searchable, sortable data organized by project and specimen

High speed: instant access to every aspect of your data

No limits: unlimited data storage, unlimited upload and download bandwidth

Document previews: view Office documents in the browser

Media streaming: play content directly in your browser

∞

Numira’s cloud-based service provides a secure web interface for customers to manage projects and explore their

datasets, media, and documents. Features of AltaNexusTM include:

Numira core-presentation-012414

Documents

Transcript of Numira core-presentation-012414