NUCLE SHELL - analytics-shop.com · 2 Ultrafast separations beyond high pressure driven UHPLC...

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www.mn-net.comMACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanyFrance:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: [email protected]

Switzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: [email protected]

Germanyand international:Tel.: +49 24 21 969-0Fax: +49 24 21 969-199E-mail: [email protected]

MACHEREY-NAGEL

EN ISO 9001: 2008CERTIFIED

USA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: [email protected]

Highest efficiency in HPLC by core-shell technology

NUCLE SHELL®

. . . we Meet your Needs

Includin

g

NEW

Phenyl−

Hexyl

phase

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Ultrafast separations beyond high pressure driven UHPLCNUCLEOSHELL® 2.7 µm

0.5 μm

0.5 μm

shell of porous silica

shell of porous silica

1.7 μm solid core of silicon dioxide

Core-shell technology Solid core of silicon dioxide, homogeneous

shell of porous silica Highestefficiencycomparedtototally

porous silica particles Particlesize2.7µm(core1.7µm), poresize90Å,specificsurface130m2/g; lower back pressure enables use on conventionalLCsystems Pressurestabilityupto600 bar

NUCLEOSHELL® modificationsTheprogramofsurfacemodificationsbasedon NUCLEOSHELL® silica now comprises the following phases:

NUCLEOSHELL®RP18 NUCLEOSHELL®Phenyl-HexylNEW! NUCLEOSHELL® PFP NUCLEOSHELL® HILIC

Several approaches have been made to achieve fast separations without losing chromato-graphic performance. HPLC columns packed with particles < 2 µm show very high efficien-cies (plates/meter) and allow the use of small-er column sizes with the positive side effect of significant solvent saving. However they gen-erate a high back pressure of the mobile phase during column runs which requires specifically designed equipment.

Core-shell particle technology from MACHEREY-NAGEL is an alternate route to gain highest column efficiency and resolution at almost the same short run time but with much lower back pressure.

NUCLEOSHELL® silica particles consist of a non-poroussolidcoreof1.7µmdiameterandaporousouter shell of0.5µm thickness.Accordingly thetotaldiameteroftheparticleis2.7µm.Withconventionalfullyporousparticlesthemasstransfer between stationary and mobile phase

usuallyresultsinpeakbroadeningathigherflowrates(C-terminvanDeemterequation).Theshortdiffusionpaths inthecore-shellparticlesreducethedwelltimeoftheanalytemoleculesinthesta-tionaryphase,sothatevenathighflowvelocitiesof the mobile phase, optimal separation results can be obtained.The van Deemter plots on page 3 demonstratehowefficiencyisaffectedbyflowrate.Incompari-sonwith fullyporoussilicas, core-shellparticlesfromvariousmanufacturersmaintaintheefficien-cyoptimum(max.plates/m)overalongrangeofincreasinglinearmobilephasevelocity.

Theoretical column efficiency (optimal conditions)Silica phase dp

[µm]L [m]

HETP [µm]

Efficiency [plates/m]

L [mm]

N Rs Analysistime

NUCLEOSHELL® 2.7 1 4 250 000 100 25 000 112 % 40 %NUCLEODUR® 1.8 1 4.5 222 222 100 22 000 105% 40%

3 1 7.5 133333 150 20 000 100% 60%5 1 12.5 80000 250 20 000 100% 100%

Benefits of core-shell technology Core-shell particles vs. totally porous silica gel

Short diffusion paths Fastmasstransfer(termCofVanDeemterequation) HighflowvelocitywithoutpeakbroadeningforfastLC

Narrow particle size distribution (d90/d10 ~ 1.1) Stable packing

High heat transfer Minimizedinfluenceoffrictionalheat EfficiencyofNUCLEOSHELL® ~ 250 000 m–1 (HETP~4µm)

3www.mn-net.com

NUCLEOSHELL®Core-shell silicaDemands on HPLC separations are constantly increasing with respect to separation efficiency, detection limits, and the time requirements for each analysis. Core-shell technology sets new standards for analyses in research and quality control.

Rs =N4

ki’+1ki’

1αα–

Rs = Resolution a = Selectivityki’ = Retention N = Theoretical plates N ∝ 1/dPdP = Particle size

Resolution RS as function of particle sizeColumns:   50 x 4 mm each

NUCLEOSHELL® RP 18, 2.7 μm NUCLEODUR® C18 Gravity, 3 μm NUCLEODUR® C18 Gravity, 1.8 μm

Eluent:  acetonitrile – water (60:40, v/v) Flow rate:   1 mL/minTemperature:   25 °CDetection:  UV, 254 nmPeaks: 1. Naphthalene2. Ethylbenzene

Better resolution at lower back pressureand shorterretention time

1

2

22

11

0.0 0.5 1.0 1.5 2.0 2.5 min

RS = 2.47104 bar

RS = 2.17190 bar

RS = 1.7188 bar

MN Appl. No. 125270

Electron microscopic image of NUCLEOSHELL® particlesUtilizingaproprietaryprocessofsyn-thesis, NUCLEOSHELL® particles ex-hibit a distinct narrow particle size distribution(d90/d10~1.1).Columnspacked with NUCLEOSHELL® core shell particles feature exceptional separa-tionefficiencieswith theoreticalplatenumbers easily comparable to totallyporous sub 2 micron particles.

Van Deemter plots

0

2

4

6

8

10

12

14

16

18

20

0 2 4 6 8 10

Term A:Term B:Term C:

Eddy diffusion Longitudinal diffusion Mass transfer

Columns:Eluent:Temperature:Sample:

50 x 4.6 mmCH3CN – H2O (70:30, v/v)

Acenaphthene25 °C

Linear velocity u [mm/s]

Plat

e he

ight

h [μ

m]

h = A + Bu--- + C · u

NUCLEOSHELL® RP 18

NUCLEODUR® C18 Gravity, 1.8 μm

Kinetex® C18

Ascentis® Express C18

Poroshell 120 EC-C18NUCLEODUR® C18 Gravity, 3 μm


Pressure drop

0

100

200

300

400

500

600

0 2 4 6 8 10 12

Kinetex® C18

Ascentis® Express C18

Poroshell 120 EC-C18

NUCLEODUR® C18 Gravity, 3 μm

dp2

Φ · LC · η · uΔp =

NUCLEOSHELL® RP 18

Columns:Eluent:Temperature:

50 x 4.6 mmCH3CN – H2O (70:30, v/v)25 °C

Pres

sure

[bar

]

Linear velocity [mm/s]

= pressure drop= flow resistance (nondimensional)= column length= viscosity= linear velocity = particle diameter

ΔpΦLCηu dp

NUCLEODUR® C18 Gravity, 1.8 μm


In direct comparison with the “con-ventional” sub 2 micron phas-es, NUCLEOSHELL® columns onlygenerate about 60% of the backpressure and can be operated with the majority of conventionalHPLC systems. In order to devel-op the maximum performance of NUCLEOSHELL® columns, we rec-ommend reducing extra column voids by using suitable capillaries(<0.15mm inner diameter) andspecially adapted detector cells.Moreover detector settings should be optimized by increasing themeasuring rate or by decrease ofthe time constant.

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Features of core-shell silica particlesStability under acidic and basic

conditionsColumn:  50 x 4.6 mm NUCLEOSHELL® RP 18, 2.7 μm

50 x 4.6 mm Kinetex® 2.6 μm C18 Eluent:  acetonitrile – 1 % TFA in H2O, pH 1 (50:50, v/v)Flow rate:  1.3 mL/min; temperature 80 °CDetection:  UV, 254 nmSample: anthracene

0

20

40

60

80

100

0 5000 10000 15000 20000 25000 30000

pH 1

NUCLEOSHELL®

Kinetex®

Column volumes

% in

ital r

eten

tion

(ant

hrac

ene)

Columns:  50 x 4.6 mm NUCLEOSHELL® RP 18, 2.7 μm 50 x 4.6 mm Ascentis® Express C18, 2.7 μm 50 x 4.6 mm Poroshell 120 EC-C18 50 x 4.6 mm Kinetex® 2.6 μm C18

Eluent:  20 mmol/L Na borate – 10 mmol/L NaOH – methanol, pH 10 (21:49:30, v/v))

Flow rate:  1.5 mL/min; temperature 40 °CDetection:  UV, 220 nmSample: toluidine

0

2000

4000

6000

8000

10000

12000

0 5000 10000 15000 20000

pH 10

Ascentis® ExpressNUCLEOSHELL®

Poroshell 120Kinetex®

Plat

es (t

olui

dine

)

Column volumes

MN Appl. Nos. 125520 / 125530

TheabovefigureshowsacolumnstabilitytestofNUCLEOSHELL®RP18atmobilephaselevelspH1andpH10comparedwiththreecompetingphases.

Acriterionforthelong-termstabilityofthecol-umn at pH extremes is the percentage decrease ofinitialretentionandinitialplates,respectively.The column can also be operated at elevated tem-peratures without loss in retention behavior, ef-ficiencyorpeaksymmetry.

Temperature stabilityStability test:Column:  50 x 2 mm NUCLEOSHELL® RP 18, 2.7 μmEluent:  A) 10 mmol/L ammonium formate – methanol 

(9:1, v/v) + 120 μL formic acid, ~ pH 4 B) 10 mmol/L ammonium formate – methanol (1:9, v/v) + 120 μL formic acid, ~ pH 4 0–100 % B in 7 min

Flow rate:  0.5 mL/minTemperature: 100 °CDetection:  UV, 220 nmPeaks:1. Phenol2. Naphthalene

0

1

2

2 4 6 8 min

38 h34 h30 h26 h22 h

MN Appl. No. 125400Efficiency test:Eluent:  acetonitrile – water (60:40, v/v)Flow rate:  0.33 mL/minTemperature:  25 °CDetection:  UV, 254 nmSample: anthracene

HETP[µm] AsymmetryStart(t=0) 5.2 0.98End(t=40h) 5.2 1.01

Batch-to-batch reproducibilityColumn:  50 x 4 mm NUCLEOSHELL® RP 18, 2.7 μmEluent:  methanol – 25 mmol/L KH2PO4 pH 7 

(70:30, v/v)Flow rate:  1 mL/minTemperature:  40 °CDetection:  UV, 254 nmPeaks:1. Uracil2. Toluene3. Ethylbenzene4. Acenapthene5. Amitriptyline6. o-Terphenyl7. Triphenylene

0 2 4 6 8 10

3

4

56

721

min

Batch 1

Batch 2

Batch 3


Uniformly shapedNUCLEOSHELL® particles com-bined with optimized bonding technology safe-guardtightlypackedcolumnsfor100%reproduc-ible results.

5www.mn-net.com

NUCLEOSHELL®

Peak capacityColumns:  100 x 4.6 mm each

NUCLEOSHELL® RP 18, 2.7 μm NUCLEODUR® C18 Gravity, 1.8 μm NUCLEODUR® C18 Gravity, 3 μm NUCLEODUR® C18 Gravity, 5 μm

Eluent:  A) acetonitrile, B) water, 40–100 % A in 4 minFlow rate:  1.5 mL/minTemperature:  25 °CDetection:  UV, 230 nmPeaks:1. Acetophenone2. Benzoin3. Propiophenone

4. Butyrophenone5. Benzophenone6. Valerophenone

0 1 2 3 4 min

3 4

56

2

1


Max. pressure [bar]

Resolution (4,5)

NUCLEOSHELL®, 2.7 µm 255 5.45NUCLEODUR®,1.8µm 450 4.14NUCLEODUR®,3µm 214 2.97NUCLEODUR®,5µm 142 2.30

Peak capacity

100125

163

216

0

50

100

150

200

250

nc:tg:W:

NUCLEODUR®5 μm 3 μm

NUCLEOSHELL®

Wtgnc = 1 + ( )

peak capacitygradient timepeak width (at baseline)

1.8 μm 2.7 μm

n c (n

orm

aliz

ed)

Thepeakcapacityisameasureofthenumberofsample analytes that can be separated on HPLCcolumns per time unit. Narrow peaks increase thepeakcapacityandefficiencyofanalyticalcol-umns. The example shows, that in comparison with totally porous NUCLEODUR® silica (1.8µm)NUCLEOSHELL®provides33%higherpeakcapac-ity.

Loading capacityNUCLEOSHELL® columns allow reliable quantifi-cationinawideanalyticaldetectionrange.Reten-tion time and peak width at 50% height remainconstant with increasing column load although core-shell particles are suspected of showing aslightly lower loadingcapacity compared to fullyporous silica materials.

Loading capacityColumn:  50 x 3 mm NUCLEOSHELL® RP 18, 2.7 μmEluent:  acetonitrile – 25 mmol/L KH2PO4, pH 3 

(70:30, v/v)Flow rate:  0.66 mL/minTemperature:  30 °CDetection:  UV, 285 nmPeaks:1. Valerophenone

9 μg

0 1 2 3 min

3 μg

0.9 μg0.3 μg

0

200

400

600

800

0 2 4 6 8 10

R2 = 0.9991

Area

Load on column [μg]

Normalized column parameters

0 2 4 6 8 10

Peak width(at 50 % peak height)

Retention time

Efficiency(at 10 % peak height)

Load on column [μg]

0

0.2

0.4

0.6

0.8

1

1.2

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NUCLEOSHELL® RP 18 Key features:

·Basedoncore-shellparticletechnol-ogyforfastandefficientHPLC

· Suitable for LC/MS and HPLC at pH extremes(pH1–11)

· Superior base deactivation, ideal for method development

Technical characteristics: Octadecylmodification,multi-

endcapped; pore size 90 Å, particle size2.7μm,carboncontent7.5%

Recommended application: Overallsophisticatedanalyticalseparations, e.g., analgesics, anti-inflammatorydrugs,antidepressants; herbicides; phytopharmaceuticals;immunosuppressants

USP L1

NUCLEOSHELL® RP 18NUCLEOSHELL®RP18isbasedoncore-shellpar-ticle technology silica. A unique derivatizationprocess generates a homogeneous surface with ahighdensityofbondedsilanes(carboncontent~7.5%).Thefollowingthoroughendcappingsup-pressesanyunwantedpolarinteractionsbetweenthe silica surface and the sample, which makes NUCLEOSHELL®RP18particularlysuitablefortheseparationofbasicandotherionizableanalytes.Theextremelyreducedsilanolactivityofthephasecanbedemonstratedbyapplyingbasicanalytes,suchas tricyclic antidepressants.The chromato-gram on page 7 shows a sharp elution profile(superior resolution!)of thesehighlypolar com-pounds with an excellent asymmetry value foramitriptylineof1.12.

Tanaka plot of NUCLEOSHELL® RP 18 Thediagrambelowunderlinesthedistincthydro-phobiccharacteristicsandthelowsilanolactivityofthestationaryphase.

0.5

1

5

10

0.5

1

1

2

0.1

0.2

1

2

hydrogen bonding capacity

capacity

ion exchange capacity pH 7.6

hydrophobicity


stericselectivity

Parameters of the Tanaka diagramCapacity=k’(pentylbenzene)Hydrophobicity=α(pentylbenzene,butylbenzene)Stericselectivity=α(triphenylene,o-terphenyl)Hydrogenbondingcapacity(silanolcapacity)=α(caffeine,phenol)Ionexchangecapacityat2differentpHvalues(2.7and7.6)=α(benzylamine,phenol)

The separation of 13 β-lactam antibiotics illus-trateshowtimeofanalysiscanbeshortenedtoafractionalpartbyusingcore-shellparticleswith-out loss of resolution at moderate back pressure.

13 β-lactam antibiotics in less than 3 minColumns:  50 x 4 mm NUCLEOSHELL® RP 18, 2.7 μm

150 x 4 mm NUCLEODUR® C18 Gravity, 5 μmEluent:  A) acetonitrile; B) 20 mmol/L KH2PO4 pH 3.5

10 % A (0.5 min) → 50 % A in 1.5 min (0.5 min 50 % A) 10 % A (3 min) → 50 % A in 9 min  (3 min 50 % A)

Flow rate:  2 mL/min, 1 mL/minPressure:  270 bar, 110 barTemperature: 25 °C Detection:  UV, 220 nmPeaks:1. Amoxicillin2. Ampicillin3. Cephalexin4. Cefotaxime5. Cefoxitin

6. Cefamandole7. Cephalothin8. Piperacillin9. Penicillin V10. Oxacillin

11. Cloxacillin12. Nafcillin13. Dicloxacillin

0.0 0.4 0.8 1.2 1.6 2.0 min

2.5 min270 bar

0 2 4 6 8 10 12 min

1

23

46

5

7 8 9

1011

1213

1

23 4

6

5

7 89

10 11

12

13


7www.mn-net.com

NUCLEOSHELL®

Tricyclic antidepressants · comparison of selectivity and resolutionColumns:  50 x 4.6 mm each 

NUCLEOSHELL® RP 18, 2.7 μm Ascentis® Express C18 Kinetex® 2.6 μm C18 Poroshell 120 EC-C18

Eluent:  methanol – acetonitrile – 25 mmol/L KH2PO4 pH 7 (22.5:22.5:55, v/v/v)

Flow rate:  2 mL/min Pressure:  224 bar, 239 bar, 248 bar, 212 barTemperature:  40 °C Detection:  UV, 220 nm

Asymmetry  (amitriptyline)

Resolution (8, 9)

NUCLEOSHELL® 1.12 3.35Ascentis® Express 2.07 1.91Kinetex® 1.33 n.a.Poroshell 1.05 1.95

1

2

3

7

89

6

5

4

0 2 4 6 8 10 12 14 16 18 min

1

2

3

4

0 1 32 min

NUCLEOSHELL®

Ascentis®

Kinetex®

Poroshell

Peaks:1. Protriptyline2. Desipramine3. Maprotiline4. Nortriptyline5. Doxepin6. Imipramine7. Amitriptyline8. Clomipramine9. Trimipramine


NUCLEOSHELL® RP 18 combines innovative silica technology and excellent surface de-activation, that outperforms conventional C18 silicas in terms of efficiency, resolution and speed. Due to the applied core-shell particle design the back pressure at elevated flow rates remains at a moderate level and per-mits the use of existing HPLC equipment in many cases. NUCLEOSHELL® RP 18 with ex-tended pH stability, low bleed characteristics in LC/MS applications and overall robustness is an ideal tool for method development and routine analysis in modern HPLC.

www.mn-net.com8

Key features: ·Basedoncore-shellparticletechnol-

ogyforfastandefficientHPLC ·Hydrophobicphasewithalternative

selectivityincomparisontoclassicalC18modifications

· Separation principle based on 2 re-tention mechanisms: π-π interactions andhydrophobicinteractions

Technical characteristics: Phenyl-Hexylmodification,multi-

endcapped; pore size 90 Å, particle size2.7μm,carboncontent4.5%; pHstability1–10;suitableforLC/MS

Recommended application: Aromaticandunsaturatedcompounds,polar compounds like pharmaceuticals, antibiotics

USP L11

Phenyl-Hexylmodified phases offer an excellentseparation efficiency especially for aromatic andunsaturatedcompoundswithelectron-withdraw-inggroups.Thecombinationofhydrophobicandπ-πinteractionsresultsinanalternativeandin-terestingselectivityprofilecomparedtheC18 or C8

Bleeding characteristics of NUCLEOSHELL® Phenyl-HexylColumns:   50 x 2 mm each

NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm Kinetex® Phenyl-Hexyl

Eluent:   A) acetonitrile, B) water;  5–95 % A in 25 min

Flow rate:   0.2 mL/minTemperature:   25 °CDetection:   MS

MN Appl. No. 126400 4 8 12 16 20 min0

1.0e8

5.0e7

cps

Temperature stability of NUCLEOSHELL® Phenyl-Hexyl

Column:   50 x 2 mm NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm

Eluent:   acetonitrile – 10 mmol/L ammonium for-mate pH 4 (50:50, v/v)

Flow rate:   0.33 mL/minTemperature:   100 °CDetection:   UV, 254 nmInjection:  0.2 μLPeaks:1. Phenol2. Naphthalene3. Anthracene


modifications. NUCLEOSHELL® Phenyl-Hexyl isbasedonanuniquesurfacebondingchemistry-therefore it is suitable for LC/MS due to low bleed-ing characteristics and offers high temperaturestabilityandpHstabilityfrom1to10.

Stability of NUCLEOSHELL® Phenyl-Hexyl at pH 10

Column:   50 x 4 mm NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm

Eluent:   acetonitrile – 50 mmol/L TEA pH 10 (60:40, v/v); pH of the mixture 10.4

Flow rate:   1 mL/minTemperature:   40 °CDetection:   UV, 254 nmInjection:  1 μLPeaks:1. Phenol2. Naphthalene3. Anthracene

Relative plate numbers

60

80

100%

0 10 20 30 40 50 h


NUCLEOSHELL® Phenyl-Hexyl is a robust phase with an alternative RP selectivity for aromatic and unsaturated analytes com-pared to classical C18 / C8 phases - it is an additional and useful tool for all chromatog-raphers.

0 1 2 min

0 h45 h

12

3

0 1 2 min

12

3

0 h60 h

NUCLEOSHELL® Phenyl-Hexyl

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NUCLEOSHELL®

Comparison of Phenyl-Hexyl phases for the separation of sulfonamidesColumns:  150 x 3 mm each

NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm NUCLEODUR® Phenyl-Hexyl, 1.8 μm NUCLEODUR® Phenyl-Hexyl, 3 μm NUCLEODUR® Phenyl-Hexyl, 5 μm

Eluent:  A) methanol,  B) 0.1 % formic acid in water,  20–80 % A in 10 min

Flow rate:  0.56 mL/minTemperature:  40 °CDetection:  UV, 254 nmInjection:  0.5 μLPeaks:1. Sulfadiazine2. Sulfachloropyridazine3. Sulfapyridine4. Sulfamerazine5. Sulfadimidine6. Sulfathiazole7. Sulfadimethoxine

On NUCLEOSHELL® Phenyl-Hexyl the resolution of the last two peaks is higher than on the fully porous 1.8 μm NUCLEODUR® Phenyl-Hexyl.

MN Appl. No. 125860 0 2 4 6 8 min

1

23

4

5

6

7

Rs26.46

Rs16.03

Rs16.83

Rs13.54

The separation of sulfonamides proves the scalability from fully porous NUCLEODUR® to NUCLEOSHELL® Phenyl-Hexyl. Hereby the core-shell silica exhibits under same conditions identi-calselectivity,narrowerpeaksandslightlyshorterretention. Thus, method transferability betweenNUCLEODUR® and NUCLEOSHELL® is guaranteed,

eitherforspeedingupyourmethodsorscalingupforpreparativerequirements.Thepyridine-phenoltest shows that NUCLEOSHELL®Phenyl-Hexylpro-videsasymmetricalpeakforpyridineandhigherresolutionincomparisontoothercore-shellbasedPhenyl-Hexylphases,whichunderlinestheexcel-lent base deactivation.

Pyridine – phenol testColumns:   50 x 2 mm each

NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm Kinetex® Phenyl-Hexyl Ascentis® Express Phenyl-Hexyl

Eluent:   acetonitrile – water (70:30, v/v)Flow rate:   0.3 mL/minTemperature:   40 °CDetection:   UV, 254 nm

1

2

min0.0 0.5 1.0

Peaks:Injection volume 0.2 μL1. Pyridine2. Phenol


www.mn-net.com10

Key features: ·Basedoncore-shellparticletechnol-

ogyforfastandefficientHPLC ·Hydrophobicphasewithalternative

selectivityincomparisontoclassicalC18modifications

· Separation principle based on 4 re-tention mechanisms: -polarinteractions(Hbonds) -dipole-dipoleinteractions -π-π interactions -hydrophobicinteractions

Technical characteristics: Pentafluorophenylpropylmodification,multi-endcapped;poresize90Å,par-ticlesize2.7μm,carboncontent~3%;pHstability1–9;suitableforLC/MS

Recommended application: Aromaticandunsaturatedcompounds,phenols, halogenated compounds, isomers, polar compounds like pharmaceuticals, antibiotics; high retention of basic compounds

USP L43

Orthogonality in selectivityFluorinatedstationaryphasesinHPLChavegainedincreasinginterestoverthelastyears.Mostcom-monrepresentativeoffluorinatedsilicaphasesisthe pentafluorophenyl modification (PFP or F5).Especially theorthogonalselectivitycomparedtotraditionalalkylphaseswidensthescopeinana-lytical HPLC. Thus NUCLEOSHELL® PFP offers anexcellent selectivity especially for highly polaranalytes, aromatic and unsaturated compounds,phenolsorhalogenatedhydrocarbons.

Halogen substitutes in molecules result often in anincreaseoftheirpolarityaccompaniedbyade-crease of typical retention characteristics in RP-HPLC.WhileatypicalC18phasejustprovideshydrophobicinteractionsbetweenstationaryphaseandanalyteNUCLEOSHELL®PFPoffersfourdifferentretentionmechanisms:polarinteractions(Hbonds),dipole-dipole interactions,π-π interactionsandhydro-phobicinteractions.Especiallythepronouncedionexchange capacity and distinct steric selectivityaretypicalforthecharacteroffluorinatedphases.

Tanaka plot of NUCLEOSHELL® PFP

0.5

1

5

10

0.5

1

1

2

0.1

0.2

1

2

hydrogen bonding capacity

capacity


hydrophobicity


stericselectivity

NUCLEOSHELL® PFP combines the benefits of core-shell technology, high stability and orthogonal selectivity. So it is a useful complementary tool for highly efficient separations especially of iso-mers, halogenated, aromatic and / or polar compounds.

β-Blockers · orthogonal selectivity of NUCLEOSHELL® PFP

Columns:  100 x 4.6 mm each  NUCLEOSHELL® RP 18, 2.7 μm NUCLEOSHELL® PFP, 2.7 μm

Eluents:  A)  acetonitrile + 0.1 % formic acid;  B) 0.1 % formic acid;  10–35 % A in 2.5 min, 35-50 % A in 2 min

Flow rate:  1.7 mL/min Temperature:  25 °C Detection:  UV, 280 nmPeaks:1. Atenolol2. Pindolol3. Metroprolol4. Labetalol5. Alprenolol6. Propranolol

0 1 2 3 4 min

1

2

3

4

6

5

1

2

3

4 5

6


NUCLEOSHELL® PFP

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NUCLEOSHELL®

Stability of NUCLEOSHELL® PFP at pH 1Columns:  100 x 4,6 mm NUCLEOSHELL® PFP, 2.7 μm

100 x 4.6 mm Kinetex® 2.6 μm PFPEluent:  acetonitrile – 0.5% TFA pH 1 (50:50, v/v)Flow rate:  1.3 mL/min Temperature: 60 °C Detection:  UV, 254 nmSample:Ethylbenzene

0

20

40

60

80

100

120

0 2000 4000 6000

NUCLEOSHELL® PFP

% initial retention

% initial plates

0

20

40

60

80

100

120

0 2000 4000 6000

Kinetex® PFP

Column volumes

% initial retention

% initial plates


MethylacetophenonesColumns:  100 x 4.6 mm NUCLEOSHELL® PFP, 2.7 μm

250 x 4 mm NUCLEODUR® PFP, 5 μm 100 x 4.6 mm Kinetex® 2.6 μm PFP

Eluent:  methanol – water (35:65, v/v)Flow rate:  1.5 mL/min, 3 mL/min, 1 mL/min, 1.5 mL/min Temperature:  35 °C Detection:  UV, 254 nmPeaks:1. o-Methylacetophenone2. p-Methylacetophenone3. m-Methylacetophenone

0 10 20 30 40 min

1

NUCLEODUR® PFP, 1 mL/min

2

3

0 5 10 min

1

2

31

NUCLEOSHELL® PFP3 mL/min

2

3

NUCLEOSHELL® PFP, 1.5 mL/min

Kinetex® PFP, 1.5 mL/min


www.mn-net.com12

NUCLEOSHELL® HILIC

NPC IC

HILIC

RPC

eluent

analyte

adso

rben

t

Key features: ·Basedoncore-shellparticle

technologyforfastandefficientHPLC · Ideal for reproducible and stable

chromatographyofhighlypolaranalytes

·Veryshortcolumnequilibrationtimes Technical characteristics:

Ammonium-sulfonicacidmodifiedsilica; pore size 90 Å, particlesize2.7μm; carboncontent1.3%; pHstability2–8.5;suitableforLC/MS

Recommended application: Hydrophiliccompoundssuchas

organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins

NUCLEOSHELL® HILICHydrophilic interaction chromatography (HILIC)is a separation technique using polar stationaryphases and organic-aqueous mobile phases. Aminimumwater content of at least 2% is indis-pensabletoprovideapermanentwaterlayerbe-tween the adsorbent surface and the organic frac-tion of the mobile phase. The sample molecules becomeseparatedinapartitionchromatography,inwhichpolaranalytesaremorestronglyretainedthanneutral,lesshydrophiliccompounds.Conse-quently,increasingtheaqueouspartinthemobilephase will diminish retention of the polar sample constituents.InthiswayHILICbehavesinversetoclassicalRPchromatography.Theparticularreten-tionprofileofHILICenablesthechromatographyof very polar and often small molecules, whichwon’t show any retention on C8 or C18 reversed phases.

Ultra-fast separations at moderate back pressureNUCLEOSHELL® HILIC is a core-shell technologybasedstationaryphasewithacovalentlybonded3-N,N-dimethylaminopropanesulfonicacidligand(pat.pend.).Thebetaine characterof the strongion-exchangerresultsinfullchargebalancingandfacilitatesfastequilibrationtimes.

CH3

CH3

SiO

2

+ N SO3 –

Good separation of polar compounds like the physiologically important substances creatineand creatinine can be achieved on NUCLEOSHELL® HILICaswellasonNUCLEODUR®HILIC,1.8µmatsimilar retention, but much lower back pressure.

Separation of creatine and creatinineColumns:   50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm

50 x 4 mm NUCLEODUR® HILIC, 1.8 μmEluent:   acetonitrile – 10 mmol/L ammonium acetate 

pH 4.0 (90:10, v/v)Flow rate:   1.7 mL/minPressure:  129 bar

180 barTemperature:  25 °CDetection:   UV, 210 nm Peaks:1. Creatinine2. Creatine

1

2

0.0 0.2 0.4 0.6 0.8 1.0min


13www.mn-net.com

NUCLEOSHELL®

Separation of catecholaminesColumns:   100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm

100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm 250 x 4 mm NUCLEODUR® HILIC, 3 μm

Eluent:   acetonitrile – 100 mmol/L ammonium formate pH 3.2 (80:20, v/v)Flow rate:   4 mL/min, 1 mL/min, 1 mL/minPressure:   395 bar, 95 bar, 116 barTemperature:   25 °CDetection:   UV, 280 nmPeaks:1. DOPAC2. Serotonin3. Dopamine4. Epinephrine5. Norepinephrine6. DOPA7. DOPS

0 5 10 min

1

2

3

4

5

6

7

12 min12 mL1

2

3

4

56

7

4.5 min4.5 mL

1

1 min4 mL

2

3

4

56

7

Core-Shell {Fully porous

(solventconsumptionper run)


Thechromatogramsshowthemethodtransferfromafullyporous3µmHILICphaseto2.7µmcore-shellsilicawithequalselectivityfeatures.Runtimehasbeencutdownto1min.Columnbackpressureremainsmodest<400bar,whilesolventdemandisreducedtolessthan35%.

NUCLEOSHELL® HILIC provides stable and reproducible chromatogra-phy, comprising all the benefits of a state-of-the-art core-shell silica.

Core-shell silica:

separation in 1 min

pressure < 400 bar

www.mn-net.com14

ApplicationsSeparation of ketones

Columns:  50 x 3 mm NUCLEOSHELL® RP 18, 2.7 μm 50 x 2 mm NUCLEODUR® C18 Gravity, 1.8 μm 125 x 2 mm NUCLEODUR® C18 Gravity, 5 μm

Eluent:  acetonitrile – water (60:40, v/v)Flow rate:  4 mL/min, 1.25 mL/min, 0.33 mL/min Pressure:  540 bar, 774 bar, 89 barTemperature:  25 °CDetection:  UV, 254 nm Injection:  1 μL, 1 mg/mL of each compound in eluentPeaks:1. Acetophenone2. Eugenol3. Propiophenone4. Butyrophenone5. Benzophenone6. Valerophenone

0 1 2 3 min

1

2

3

45 6

12

3

4

56

12

3

45

6

1

2

3

4

56

1

2

3

45

6

NUCLEOSHELL®

0.0 0.2 0.4 min

NUCLEODUR®

1,8 μm


Tricyclic antidepressantsColumns:   50 x 4.6 mm NUCLEOSHELL® RP 18, 2.7 μm

250 x 4.6 mm fully porous C18, 5 μmEluent:   methanol – acetonitrile – 25 mmol/L KH2PO4

pH 7 (22.5:22.5:55, v/v)Flow rate:   2 mL/min, 1.3 mL/minPressure:   224 bar, 190 barTemp.:   40 °CDetection:   UV, 220 nm 

0 50 100 150 min

1

2

3

4 5

6 78

9

0 5 10 15 min

1

18 min36 mL

180 min234 mL

2

3

4

5

6 78

9

20

Peaks:1. Protriptyline2. Desipramine3. Maprotiline4. Nortriptyline5. Doxepin6. Imipramine7. Amitriptyline8. Clomipramine9. Trimipramine


time sav

ing

90 %

eluent s

aving

85 %

15www.mn-net.com

NUCLEOSHELL®

Acidic pharmaceuticalsColumns:   50 x 4 mm NUCLEOSHELL® RP 18, 2.7 μm

150 x 4 mm fully porous C18, 5 μmEluent:   A) acetonitrile, B) 25 mmol/L KH2PO4 pH 7, 

25–40 % A in 2.2 min, 25–40 % A in 20 minFlow rate:   1.5 mL/min, 0.5 mL/minPressure:   219 bar, 92 barTemp.:   20 °CDetection:   UV, 215 nm Peaks:1. Ketoprofen2. Fenoprop3. Fenoprofen4. Flurbiprofen

5. Ibuprofen7. Carprofen8. Diclofenac9. Meclofenamic acid

0 1 2 min

12

34

5

6

7

8

2.2 min3.3 mLRs (2, 3) = 1.69

0 5 10 15 min

1

2

34

5

6

7

820 min10 mLRs (2, 3) = 1.70


Separation of steroidsColumns:  50 x 3 mm NUCLEOSHELL® RP 18, 2.7 μm

125 x 3 mm NUCLEODUR® C18 Gravity, 3 μmEluent:  A) acetonitrile, B) water

30–80 % A in 1 min (0.4 min 80 % A) 30–80 % A in 5 min (2 min 80 % A)

up to 90 % time saving

66 % solvent saving

Flow rate:  2 mL/min 1 mL/min

Pressure:  350 bar 280 bar

Temperature:  25 °C Detection:  UV, 240 nmInjection:  1 μL, 1 mg/mL of each compound in eluent

0 21 3 4 5 min

32

1 4

5

6

7

8 93

4

5

61.4 min2.8 mL

7 min7 mL

2

1 7

8 9

Peaks:1. Estriol2. Prednisolone3. Cortisone4. Estradiol5. Testosterone6. Estrone7. 6a-Methyl-11b-hydroxyprogesterone8. 6a-Methyl-17a-hydroxyprogesterone9. Progesterone


www.mn-net.com16

Non-steroidal anti-inflammatory drugsColumns:   50 x 4.6 mm each

NUCLEOSHELL® RP 18, 2.7 μm Ascentis® Express C18

Eluent:   acetonitrile – 20 mmol/L KH2PO4 pH 2.5 (40:60, v/v)

Flow rate:   2.5 mL/minPressure:   268 bar, 281 barTemperature:   22 °CDetection:   UV, 230 nmInjection:  1 μL, 1 mg/mL of each compound in eluent

0 1 2 3 min

3

1

2

6

45 8

Rs = 1.85 Rs = 1.59

9 10 1112

7

Rs = 1.25 Rs = 1.14

Peaks:1. Acetylsalicylic acid2. Sulindac3. Piroxicam4. Suprofen5. Tolmetin6. Naproxen7. Diflunisal8. Fenoprofen9. Flurbiprofen10. Niflumic acid11. Indomethacin12. Ibuprofen


Steviol glycosidesColumns:   150 x 4.6 mm

NUCLEOSHELL® RP 18, 2.7 μmEluent:   acetonitrile – 10 mmol/L NaH2PO4 pH 2.6 

(32:68, v/v)Flow rate:   1.0 mL/minTemperature:   40 °CDetection:   UV, 210 nmInjection:  5 μL

0 2 4 6 min

2

3

5

1 4 6 7 8 9

Peaks:1. Rebaudioside D2. Rebaudioside A3. Stevioside4. Rebaudioside F5. Rebaudioside C6. Dulcoside A7. Rubusoside8. Rebaudioside B9. Steviolbioside


Phosphonic acid herbicidesColumns:   100 x 2 mm

NUCLEOSHELL® RP 18, 2.7 μmEluent:   A) acetonitrile, 

B) 50 mmol/L ammonium acetate;  5–50 % A in 3.7 min,  50–95 % A in 0.6 min (2 min 95 % A), 95–5 % A in 0.5 min (2 min 5 % A)

Flow rate:   0.5 mL/minTemperature:   30 °CDetection:   MSInjection:  5 μLPeaks:1. Glyphosate (167 ng/mL)2. Glufosinate (16.7 ng/mL)3. AMPA (167 ng/mL)

GlufosinatetR = 2.34 min

GlyphosatetR = 2.09 min

AMPAtR = 2.88 min

2

1

3

2

3 min

Courtesy of KUDZU SCIENCE, Illkirch, France


good

selectiv

ity

and re

solution

Applications

17www.mn-net.com

NUCLEOSHELL®

β-Lactam antibioticsColumn:  50 x 2 mm

NUCLEOSHELL® Phenyl-Hexyl, 2.7 μmEluent:  methanol – 10 mmol/L ammonium formate, 

pH 3 (50:50, v/v)Flow rate:  0.45 mL/min Temperature:  40 °C Detection:  MSInjection:  1 μLPeaks:1. Cefoxitin2. Cloxacillin3. Nafcillin4. Dicloxacillin

0 1 2 3 4 min

1

2

3

4


AntihistaminesColumn:  100 x 3 mm

NUCLEOSHELL® Phenyl-Hexyl, 2.7 μmEluent:  A) methanol, 

B) 10 mmol/L ammonium formate, pH 2.8; 17.5 % A (2.5 min) → 65 % A in 1.5 min → 75 % A in 1.5 min (4.5 min 75 % A)

Flow rate:  0.6 mL/min Temperature:  40 °C Detection:  MSInjection:  0.5 μLPeaks:1. 4-Acetaminophenol2. Pseudoephedrine3. Codeine4. Chlorpheniramine5. Dextromethorphan

1 2 3 4 5 6 7 8 min0

1

23

4

5


BenzodiazepinesColumn:  50 x 2 mm

NUCLEOSHELL® Phenyl-Hexyl, 2.7 μmEluent:  A) acetonitrile, 

B) 20 mmol/L ammonium formate, pH 6.4; 25–55 % A in 10 min

Flow rate:  0.33 mL/min Temperature:  25 °C Detection:  MSInjection:  2.5 μL

0 1 2 3 4 5 6 7 8 9 min

1

2

3

4 5

6

Peaks:1. Oxazepam2. Chlordiazepoxide3. Alprazolam4. Trazodone5. Nordiazepam6. Diazepam


www.mn-net.com18

ApplicationsPhthalates

Column:  100 x 3 mm NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm

Eluent:  A) acetonitrile – methanol (50:50, v/v),  B) 0.1 % formic acid in water;  75 –98 % A in 3.5 min (2.5 min 98 % A)

Flow rate:  0.75 mL/min Temperature:  20 °C Detection:  MSInjection:  0.5 μL

0 1 2 3 4 5 min

1

2

3

4

5

6

7

8

Peaks:1. Dimethyl phthalate2. Dipropyl phthalate3. Diphenyl phthalate4. Dibutyl phthalate5. Benzyl butyl phthalate6. Diheptyl phthalate7. Di-n-octyl phthalate8. Di-i-decyl phthalate


AflatoxinsColumn:  50 x 2 mm NUCLEOSHELL® PFP, 2.7 μmEluent:  methanol – 10 mmol/L ammonium acetate 

(45:55, v/v)Flow rate:  0.33 mL/min Temperature:  25 °C Detection:  MSInjection:  0.1 ng eachPeaks:1. Aflatoxin G22. Aflatoxin G13. Aflatoxin B24. Aflatoxin B1


10 2 3 min

1

2

3

4

Beta- and dexamethasoneColumns:  50 x 4 mm NUCLEOSHELL® PFP, 2.7 μm

100 x 4.6 mm NUCLEODUR® PFP, 5 μmEluent:  acetonitrile – water (20:80, v/v)Flow rate:  1.5 mL/min

1.3 mL/min Temperature:  30 °C Detection:  UV, 260 nmPeaks:1. Betamethasone2. Dexamethasone

0 5 10 15 20 min

1

2

1

2


19www.mn-net.com

NUCLEOSHELL®

PhenolsColumns: 100 x 4.6 mm NUCLEOSHELL® PFP, 2.7 μm

100 x 4.6 mm NUCLEODUR® PFP, 5 μmEluent:  acetonitrile + 0.1 % formic acid – 0.1 % formic acid 

(35:65, v/v)

0 2 4 6 8 10 min

12

3

4

5

6

7 8

9

10

11

0 1 2 min

12

3

4

5

6

7 8

9

10

11

Flow rate:  4 / 1.3 mL/min Temperature:  35 °C Detection:  UV, 280 nm

Peaks:1. o-Cresol2. m-Cresol3. 3,4-Dimethylphenol4. 3,5-Dimethylphenol5. 2,5-Dimethylphenol6. 2,6-Dichlorophenol

7. 2,3-Dichlorophenol8. 2,4-Dichlorophenol9. 3,4-Dichlorophenol10. 2,4-Dibromophenol11. 3,5-Dichlorophenol


Water-soluble vitaminsColumn:   100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent:   A) acetonitrile – 100 mmol/L ammonium 

acetate pH 3.2 (90:10, v/v), B) water;  4 % B (1 min) → 20 % B in 1.6 min  (0.7 min 20 % B)

Flow rate:   2 mL/minPressure:  218 barTemperature: 25 °CDetection:   UV, 260 nm Peaks:1. PABA (p-aminobenzoic acid)2. Nicotinamide3. Vitamin B6 (pyridoxine)4. Riboflavin5. Nicotinic acid6. Vitamin C (ascorbic acid)7. Vitamin B1 (thiamine)8. Folic acid9. Vitamin B12 (cyanocobalamine)

0 1 2 3 min

12

3 4

5

67

8

9


Anions and cationsColumn:   100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent:   A) 30 mmol/L ammonium formate pH 3, 

B) acetonitrile  80 % B (3 min) → 20 % B in 7 min

Flow rate:   1.5 mL/minPressure:  200 barTemperature: 40 °CDetection:   CAD (Nebulizer: 35 °C) Peaks:1. Nitrate2. Chloride3. Potassium4. Sodium5. Phosphate6. Sulfate

0 1 2 3 4 5 6 min

12

3

4

5

6


www.mn-net.com20

ApplicationsMetformin

Columns:   50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm 50 x 4 mm NUCLEODUR® HILIC, 1.8 μm

Eluent:   acetonitrile – 10 mmol/L ammonium acetate pH 3.2 (75:25, v/v)

Flow rate:   3 mL/min 1.5 mL/min

Pressure:   202 bar 167 bar

Temperature:  25 °CDetection:   UV, 218 nm 

1

2

NUCLEOSHELL®

NUCLEODUR®

1

2

0.0 0.5 1.0 min

Peaks:1. Dicyandiamide2. Metformin


5-FluorouracilColumn:   50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent:   acetonitrile – 10 mmol/L ammonium acetate 

(95:5, v/v)Flow rate:   2.5 mL/minPressure:  119 barTemperature:  25 °CDetection:   UV, 254 nmPeaks:1. 5-Fluorouracil2. Uracil

1

2

0.0 0.2 0.4 min


Analysis of an energy drinkColumn:   100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent:   acetonitrile – 100 mmol/L ammonium acetate 

pH 5.0 (90:10, v/v)Flow rate:   1.7 mL/minPressure:  126 barTemperature: 35 °CDetection:   UV, 254 nm Peaks:1. Caffeine2. Niacinamide3. Pyridoxine4. Benzoic acid5. Sorbic acid6. Riboflavin

1

2

34

5

6

0 1 min


good

selectiv

ity and

peak sh

ape

21www.mn-net.com

NUCLEOSHELL®

Acrylamide and analogsColumn:   50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent:   acetonitrile – 0.1% formic acid in water 

(98:2, v/v)Flow rate:   1.0 mL/minTemperature:  25 °CDetection:   UV, 200 nm Injection:  1 μL, 1 mg/mL of each compound in eluent Peaks:1. Acrylamide2. Methacrylamide3. Methacrylic acid

3

2

1

0.0 0.5 1.0 min


NucleotidesColumn:   50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluents:   A) acetonitrile; B) 100 mmol/L ammonium 

acetate pH 5.35; 87.5–60 % A in 18 min Flow rate:   2.2 mL/minTemperature:  30 °CDetection:   UV, 259 nm Injection:  1 μL, 1 mg/mL of each compound in eluent Peaks:1. Uridine2. Adenosine3. Cytidine4. Cyclic adenosine monophosphate5. Uridine monophosphate6. Adenosine monophosphate7. Inosine monophosphate8. Cytidine monophosphate

3 4

5

8

7

6

2

1

0 2 4 6 8 10 12 min


Amino acidsColumn:   50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent:   acetonitrile – 100 mmol/L ammonium 

acetate pH 4.0 (80:20, v/v)Flow rate:   1.5 mL/minPressure:  105 barTemperature:  25 °CDetection:   UV, 215 nm Peaks:1. Phenylalanine2. Phenylglycine3. Tyrosine4. Histamine

4

3

2

1

0.0 0.4 0.8 1.2 1.6 2.0 min


www.mn-net.com22

Packed columns · Ordering informationEC standard columns for analytical HPLC

Analyticalcolumnsystemmadeofstainless steel M8outerthreadsonbothendsCombination of sealing element and veryfine-meshedstainlesssteelscreen,PTFEringandfittingadapterColumnheadsSW12,withinnerthreadsM8x0.75andUNF10-32(=1/16”fitting)EC column hardware guarantees pressure stabilityof1200bar-henceECcolumnsaresuitableforU-HPLCapplications(ultrafastHPLC)andallmodernHPLCsystems.

Asscrew-onguardcolumnsystemwerec-ommend the Column Protection System used with EC guard column cartridges with 4mmlength(seenextpage).

EC analytical columns Allphases:poresize90Å,particlesize2.7µm; eluent in column CH3CN – H2O

Length→ 50 mm 100mm 150mm EC guard columns*

NUCLEOSHELL® RP 18, 2.7 µm octadecylmodification,multi-endcapped,7.5%C2mmID 763132.20 763134.20 763136.20 4x2mm:763138.203mmID 763132.30 763134.30 763136.30 4x3mm:763138.304mmID 763132.40 763134.40 763136.40 4x3mm:763138.304.6mmID 763132.46 763134.46 763136.46 4x3mm:763138.30

NUCLEOSHELL® Phenyl-Hexyl, 2.7 µm phenyl-hexylmodification,multi-endcapped,4.5%C2mmID 763732.20 763734.20 763736.20 4x2mm:763738.203mmID 763732.30 763734.30 763736.30 4x3mm:763738.304mmID 763732.40 763734.40 763736.40 4x3mm:763738.304.6mmID 763732.46 763734.46 763736.46 4x3mm:763738.30

NUCLEOSHELL® PFP, 2.7 µm pentafluorophenylmodification,multi-endcapped,~3%C2mmID 763532.20 763534.20 763536.20 4x2mm:763538.203mmID 763532.30 763534.30 763536.30 4x3mm:763538.304mmID 763532.40 763534.40 763536.40 4x3mm:763538.304.6mmID 763532.46 763534.46 763536.46 4x3mm:763538.30

NUCLEOSHELL® HILIC, 2.7 µm ammonium–sulfonicacidmodification,1.3%C2mmID 763332.20 763334.20 763336.20 4x2mm:763338.203mmID 763332.30 763334.30 763336.30 4x3mm:763338.304mmID 763332.40 763334.40 763336.40 4x3mm:763338.304.6mmID 763332.46 763334.46 763336.46 4x3mm:763338.30

*ECguardcolumnsrequiretheColumnProtectionSystemCartridgeHolderREF718966(seeright). ECcolumnsinpacksof1,guardcolumnsinpacksof3

23www.mn-net.com

NUCLEOSHELL®

Column Protection SystemInnovative and universal screw-on guard column holder systemSuitable for all analytical HPLC columns with 1/16” fittings

CartridgesfilledwithspecifiedNUCLEOSHELL®,NUCLEODUR®, and NUCLEOSIL® HPLC adsorbents

Idealprotectionforyouranalyticalmaincolumn →significantincreaseincolumnlifetime

Minimized void volume → suitable also for ultra fast HPLC

Special ferrules →pressurestabilityupto1034bar(15000psi)

Visualcontaminationcheck→in-timechanging of the guard column

Guardcolumnlength4mm,ID2mm(formaincolumnswith2mmID)orID3mm(formaincolumnswith3,4and4.6mmID)

UNIVERSALRPguardcolumnsavailableforall HPLC columns under RP conditions

Content of the Column Protection System

Description REF

Column Protection System 718966

Details Content

Cartridge Holder 1

Replacementcapillaries(0.12mmID) 2

Ferrules 3

Wrenches 2

Manual 1

Replacement parts for the Column Protection System • Ordering information

Description Pack of REF

Ferrules 5 718967ReplacementconnectorincludingO-ring 1 718968Stainlesssteelcapillaries0.12mmID,nutsandmetalferrules 3 718969Stainlesssteelcapillaries0.18mmID(forhigherflowrates),nutsandmetalferrules 3 718971Wrench(size12and14mm) 1 718970EC4/2UNIVERSALRPguardcolumn(formaincolumnswith2mmID) 3 728777.20EC4/2UNIVERSALRPguardcolumn(formaincolumnswith2mmID),valuepack 9 728778.20EC4/3UNIVERSALRPguardcolumn(formaincolumnswith3,4and4.6mmID) 3 728777.30EC4/3UNIVERSALRPguardcolumn(formaincolumnswith3,4and4.6mmID),valuepack 9 728778.30

Visual Contamination Check

The cartridge is fitted with a special filter membrane.

Ifthesilvermembraneiscontaminated(brightordarkdiscoloration),itisadvisable to replace the cartridge.

If the contaminants are colorless, replace the cartridge as soon as the pressure rises or the chromatographic performance decreases.

Trademarks: NUCLEOSHELL®,NUCLEODUR®(MACHEREY-NAGELGmbH&Co.KG,Germany),Ascentis®(Sigma-AldrichCo.,USA),Kinetex®(PhenomenexInc.,USA),Poroshell(AgilentTechnologiesInc.,USA) Imagecredits:©by-studio/Alexey(Fotolia.com)

www.mn-net.com

www.mn-net.comMACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanyFrance:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: [email protected]

Switzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: [email protected]

Germanyand international:Tel.: +49 24 21 969-0Fax: +49 24 21 969-199E-mail: [email protected]

MACHEREY-NAGEL

EN ISO 9001: 2008CERTIFIED

USA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: [email protected]

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