NSA08 Roberts Scallop
description
Transcript of NSA08 Roberts Scallop
Bay scallop genetic diversity and population structure in
Massachusetts
Steven RobertsSchool of Aquatic and Fishery Sciences
University of Washington
Outline
•Beginning
•Marker Development
•Populations / Enhancement
•Conclusions
• ESTs = 7057
• CoreNucleotides = 85
• Contigs 845
• Singletons 2746
• Unique Seqs 3591
Individual clones sequencedD-hinge 671Met 61Set 932Adductor 367Gonad 58
8% sequences <200 bpAverage length - 719 bp970 singletons217 contigs
NCBI Present StatusLibrary Characteristics
Enhancement
Population Differences
Self-assignment
Only Adults Random = 11%Chatham = 60%
Assessing Enhancement
private alleles showing up post enhancement
Summary
• Identify markers that effectively identify all potential parent populations
• functional markers (SSR-ESTs, SNPs)
• Isolation coupled with life history can dramatically affect genetic diversity
• Temporal (yearly) variation is interesting and critical for understanding diversity