Not for Medical Diagnostic Use

The Spectrum Compact CE System is an integrated and efficient

instrument that brings you the independence to perform Sanger

sequencing and fragment analysis in your laboratory, under your

control and at your convenience.

It is designed for use with existing sequencing chemistries using

fluorescently labeled dideoxynucleotide triphosphates and 4-, 5- and

6-dye STR kits from Promega, and other commercially available kits.

Capillary Electrophoresis Just Got Personal

The Spectrum Compact is the solution for academic, clinical

research and forensic laboratories looking to take analyses into their

own hands. This innovative instrument provides a high return on

investment, conserves valuable bench space and allows flexibility in

sample batch sizes.

The Spectrum Compact CE System is:

A personal, benchtop instrument

• Conforms to your workflow

» No waiting to batch samples or send samples to an outside lab

• Enables a more efficient workflow

» 32 sample capacity using four 8-well strip tubes

» 4-capillary array

• Saves time and reduces reagent waste

Capable of Sanger sequencing and fragment analysis

• Compatible with commercially available sequencing kits

» Uses fluorescently labeled dideoxynucleotide triphosphates

» Optimized for a broad range of applications including:

• De novo sequencing

• NGS confirmation

• Resequencing

• Mutation detection

• Mitochondrial sequencing

• Designed to work with all common STR kits on the market

» Compatible with 4-, 5- and 6-dye STR chemistries

» Other uses include:

• Microsatellites

• PCR sizing

• SNP genotyping

Not for Medical Diagnostic Use

Integrated Touchscreen Operation

The Spectrum Compact Control Software provides a simple user

interface with a clear display of useful features including run set

up, consumables and array usage information, as well as system

maintenance reminders.

• Easy-to-use operational workflow wizard

• Operations run directly on the instrument touch screen

• Ability to monitor run progress and view analyzed results while a

run is in progress

• Export files are compatible with commercially available data

analysis software such as GeneMarker®, Mutation Surveyor® and

GeneMapper®.

Small-Footprint Advanced Multiplexing

The Spectrum Compact offers the high performance of a 6-dye

compatible CE instrument, but at an affordable price point.

Electropherogram of 1ng of 2800M control DNA analyzed with PowerPlex®

Fusion 6C System run on prototype Spectrum Compact CE System. Injection was

performed at 1.2kV for 8 seconds.

Electropherogram of the PowerPlex® Fusion 6C System allelic ladder run on

prototype Spectrum Compact CE System. Injection was performed at 1.2kV for

8 seconds.

1401

0TA

600060 100 140 180 220 260 300 340 380 420 460 500

50004000300020001000

0

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50004000300020001000

0

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0

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50004000300020001000

0

600060 100 140 180 220 260 300 340 380 420 460 500

50004000300020001000

0

AMEL D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E

D16S539 D18S51 D2S1338 CSF1PO Penta D

TH01 vWA D21S11 D7S820 D5S818 TPOX

D8S1179 D12S391 D19S433 SE33 D22S1045

DYS391 FGA DYS576 DYS570

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1TA

300060 100 140 180 220 260 300 340 380 420 460 500

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0

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0

AMEL D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E

D16S539 D18S51 D2S1338 CSF1PO Penta D

TH01 vWA D21S11 D7S820 D5S818 TPOX

D8S1179 D12S391 D19S433 SE33 D22S1045

DYS391 FGA DYS576 DYS570

Not for Medical Diagnostic Use

Single-base Resolution

Spectrum Compact achieves a 1bp resolution as can be seen in the D1S1656 and

D2S441 loci in the PowerPlex® Fusion 6C System Allelic Ladder. Injection was

performed at 1.2kV for 8 seconds.

1401

2TA

2000150 170 190 210 230 250 270

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1600

1400

1200

1000

800

600

400

200

0

D1S1656 D2S441 D10S1248

A personal, affordable benchtop instrument

for Sanger sequencing and fragment

analysis applications

Accurate and Fast Sequencing

Spectrum Compact supports Sanger sequencing applications used as

orthogonal technology for verification of NGS base calls and DNA and

gene sequencing required in a variety of genomic applications.

Panel A

Panel B

Representative portion of an electropherogram (Panel A) generated using the Applied

Biosystems BigDye® v3.1 Kit, Polymer 7, and Hitachi base calling software; (Panel B)

whole data file.

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8TA

5,200

260 270 280 290 300 310 320 330

5,300 5,400 5,500 5,600 5,700 5,800 5,900 6,000 6,100

G A A G A C C C T T A C T A C T G T C C A A G G G A T C G C T G A T G A T T A C G A T A A A A A G A A A C T A G T G A A G G C G T T T A A G A A A A A G T T T G C C T G

1400

9TA

14,00013,00012,00011,00010,0009,0008,0007,0006,0005,0004,0003,0002,000 15,000

Not for Medical Diagnostic Use

Spectrum Compact Consumables and Accessories

Plug-and-play, prefilled consumables bring capillary electrophoresis

capabilities to the hands of any researcher in the laboratory regardless

of skill level or expertise.

Not only is the Spectrum Compact simple to set up and operate,

related consumables such as prefilled buffer and polymer are available

in convenient packaging. Accessories such as plates, septa mats

and formamide are also available for purchase. All consumables and

accessories use 2D barcoding to track key information.

The Spectrum Compact System Control Software enables real-time tracking of key

reagents and consumables.

Spectrum Compact Consumables

Spectrum Compact Capillary Array

• 4-Capillary, 36cm

• Barcoded for consumable tracking

Spectrum Compact Cathode Septa Mat

• 8-well septa mat specific for the Spectrum Compact cathode

buffer cartridge

Spectrum Compact Polymer4

• 64 samples

• Barcoded for consumable tracking

Spectrum Compact Polymer7

• 64 samples

• Barcoded for consumable tracking

Spectrum Compact Plate Base & Retainer, 32-Well

Spectrum Compact Buffer

• Prefilled, ready-to-use anode and cathode cartridges

• Barcoded for consumable tracking

Spectrum Compact Wash Solution

• Ready-to-use, prefilled syringe for system washes

• Barcoded for consumable tracking

Universal Consumables

Strip Cap, 8-Well

• 8-strip domed caps for PCR plates and tubes to prevent

evaporation and contamination

• Certified RNase- and DNase-free

Strip Tubes, 8-Well

• 8 strip 0.2ml PCR tubes

• Certified RNase- and DNase-free

Strip Septa Mat, 8-Well

• Autoclavable and reusable up to 5 times for a more cost-

effective alternative to other sealing methods

Formamide, CE-Grade, 25ml

• Highly deionized, low conductivity, <5μS

• Used for electrokinetic injection on CE instruments

Not for Medical Diagnostic Use

» 4-capillary array

» Polymer

» Anode and cathode buffers

» Wash solution

» Cathode septa mats

» Plate bases and retainers

» Formamide

» Reagents necessary for installation

Promega Support

Promega chemistries and Spectrum Compact are provided as an

integrated system for delivering optimized results for sequencing and

fragment analysis. On-site installation and training for the instrument,

software and kits is included with the purchase of the instrument.

Additionally, flexible service and support options will be available,

depending on the requirements of your laboratory.

Promega offers service and support plans for preventative

maintenance, on-site service and validation plans to ensure minimal

interruption of your laboratory operations.

• Phone-based and on-site consultation

• On-site bench work tailored to your requirements

• Rapid turnaround of data analyses and summary reports

System Components

The Spectrum Compact CE System is supplied as follows:

• Capillary electrophoresis instrument

• Integrated touchscreen

• On-site service for installation and initial user training

Dimensions 40cm W x 70cm D x 60cm H

Weight 41kg

Main Power Voltage 95-240VAC, 50-60Hz (global compatibility)

15 amps

Operating Environment 15°C – 30°C

Sample Capacity 1-32 samples (four 8-well strip tubes)

Injection Throughput 4-capillary array

Capillary Array Length 36cm

Service and Warranty Service installation

On-site application training

Extended service contracts will be available separately–please inquire.

Validation Support Available separately–please inquire.

Expected Specifications

40cm

60cm

70cm

Not for Medical Diagnostic Use

For Research Use Only. Not for use in diagnostic procedures.

PowerPlex is a registered trademark of Promega Corporation.

GeneMarker and Mutation Surveyor are registered trademarks of SoftGenetics.

ThermalSeal is a registered trademark of Excel Scientific, Inc.

GeneMapper is a registered trademark of Thermo Fisher Scientific.

Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.

ALL PRODUCTS, PRODUCT SPECIFICATIONS AND DATA ARE SUBJECT TO CHANGE WITHOUT NOTICE TO IMPROVE RELIABILITY, FUNCTION OR DESIGN OR OTHERWISE.

© 2017 Promega Corporation. All Rights Reserved. Printed in USA 2/17. 34717955. Part #BR292

To learn more, visit: www.promega.com/SpectrumCompact