@No tll bca and biofertilizers 2012 09 04 a
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Transcript of @No tll bca and biofertilizers 2012 09 04 a
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Modern Biocontrol Agents and Biofertilizers
Prof. Mark Laing
Plant Pathology, UKZN & Plant Health Products (Pty) Ltd
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Infected Ant
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The Future is Here, for BCAs
1. Fewer pesticides available
2. A wider range of BCAs
3. Better formulations, shelf-life, simplicity
4. Biofertilizers:
• N fixing – nodules and free-living
• Mobilization of P and micronutrients
• Buffering abiotic stress:
• Tolerance of heat, cold, drought, waterlogging, soil toxins, toxic metals
5. Soluble silicon - a powerful tool to add
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Beneficial Microbes for Agriculture – available of coming soon !
1. Biocontrol Agents to control • Diseases, pre- and post-harvest - most • Pests - most • Nematodes – coming now
Added benefit – Growth stimulation esp. seed and roots
2. Biofertilizers to enhance fertilization • N fixation – Rhizobium, Azotobacter, etc. • P mobilization • Micronutrient mobilization – Mo, Bo, Zn, Fe
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What growers need: Registered, commercial BCA products: effective, affordable, stable, simple
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Field Crop Soil Problems • Seed death, damping off and uneven stand
(sub-lethal infection)
• Root diseases of adult plants
– Cause - Pythium, Rhizoctonia, Fusarium spp., Ggt (take-all)
– Solution - seed treat with Trichoderma (e.g., Eco-T from PHP)
• It stimulates early germination
• It colonizes the emerging root
• It stimulates more lateral root formation
• It kills incoming pathogens and nematodes
• It degrades allelochemicals in the soil
• It buffers versus abiotic stress factors
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Biocontrol of Rhizoctonia on
cucumber seedlings
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Trichoderma coils, penetrates, kills. Enzymes digest cell walls and contents. Feeding like a spider on a fly.
D
T
R
B
TP
R
C
T
C
R
A
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0
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Untre
ated
Cru
izer o
nly
Eco
-T o
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Cru
izer +
Eco
-T
Tricho
Plus on
ly
Cru
izer +
Tric
hoPlus
avg
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ot
weig
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Effects of treatments on root wet weight
A Maize Trial with 2 Trichodermas & Cruiser
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Mechanisms of Biocontrol
1. Hyper-parasitism (eats fungus)
2. Antibiosis (chemicals that attack pathogens)
3. Competitive displacement (eats nutrients first)
4. Triggering resistance in host (SAR)
5. Binds iron needed by pathogen (releases binding agents, siderophores)
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Kenyan Lisianthus with Fusarium oxysporum root rot
No treatment Trichoderma applied weekly
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Survival (%)
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Con
trol
Rhi
zoc
T d
r +
Rhi
zoc
T s
t +
Rhi
zoc
Treatment
Sur
viva
l (%
)
Total Dry Weight
0
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Rhiz
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r +
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Rhiz
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TreatmentDry
Weight
(g)
Biocontrol of Rhizoctonia on
cucumber seedlings
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Foliar Diseases – 2 examples
• Rust biocontrol– fungus Sphaerellopsis filum
attacks pustules and stops carryover – isolate, screen,
register. Studies on biology, epidemiology, efficacy over
seasons, economics of its use, culture conditions.
• Powdery mildew biocontrol - Ampelomyces
quisqualis (AQ). AQ10 was commercialized previously.
PHP has worked out its production but it needs
registration trials.
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A rust pustule colonized by S. filum
S. filum – black spore
capsules in the old rust
pustule
A new rust pustule
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Infection of wheat rust spore by S. filum (Plachecka 2005)
17
Rust
Spore
Rust Spore
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Ampelomyces quisqualis attacking grape powdery mildew, U. necator
18
A pycnidium of A. quisqualis
developed inside a spore of pm,
showing the powdery mildew colony
on a grape leaf in the background.
Photos - D. Gadoury
Spores of A. quisqualis emerging
from a pycnidium of A. quisqualis,
formed in place of a pm conidium
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Post-Harvest vs Moulds and Insects
• Eco-77 – colonize flowers of wheat and maize to stop Aspergillus & Fusarium (source of mycotoxins)
• Yeasts: e.g. YieldPlus, B13
• Control of weevils & moths (USDA too)
– Beauveria bassiana
– Bacillus thurungiensis
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Untreated Control
Yeast B13
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Rice Weevil (Sitophilus oryzae) infected
with Beauvaria bassiana Rice Weevil (Sitophilus oryzae) infected
with Beauvaria bassiana
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Insect Pests • Aphids – Conidiobolus ?
• Caterpillars – Viruses, Btk, Bb
• Bollworm - viruses
• Red spider – Beauveria bassiana
• Thrips – Bb
• Beetles, weevils – Bb, EPNs
• Whitegrubs – B. brognartia, Bb, Bt
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Beauveria bassiana parasitizing
army worm larvae
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Beauveria-infected
white grub adult
Beauveria-infected
black vine weevil
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Dead red spider mites after Eco-Bb x K silicate
on brinjal (Gatarahiya & Laing, 2008)
0
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0 1 2Beauveria rate (g l-1)
% d
ead
mit
es
(ad
ult
s+
juven
iles)
No Si
With Si
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Bollworm on Wheat Infected by Virus
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Beetle grub filled with EPN’s
Pictures taken from the University of Florida extension service web-site
http://edis.ifas.ufl.edu/IN468
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Entomopathogenic nematode entering an insect larva
EPN
Larva
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Nematode (Eelworm) Biocontrol
• Trichoderma (Eco-T) is suppressive
• Nematode trapping fungi – Clonostachys rosae eats nematode larvae
• Bacillus thurungiensis strains kill if eaten
• Bayer – Votivo = Bacillus firmus
• Products that eat nematode eggs – to kill all eggs in top 300mm of soil, requires 2000 kg / ha vs recommended 2kg / ha
• Rotate with a Brassica crop (e.g., canola, cabbage) – mustard gases released
• Nematodes in sheep and goats !
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Nematode attacked by a nematode-trapping fungus
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1. Eliminates sub-lethal pathogens
2. Plant hormones released
3. Mineralization – P, Mo dissolved
4. Detoxification – allellochemicals and metals
5. Environmental buffering
Growth Stimulation by Trichoderma:
Modes of action
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No
Treatment
Trichoderma
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Nutrient uptake can be enhanced by alterations to root structure
Untreated
1.8 area units
Eco-T seed treatment
3.2 area units
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Eco-T treated maize root
numerous root hairs Untreated maize root
Few root hairs
Nutrient uptake is enhanced by increased number of root hairs
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Root and shoot lengths of perenial
ryegrass (69 days)
0
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Untr
eate
d
Tre
ate
d
Untr
eate
d
Tre
ate
dFeb planting March planting
Len
gth
(m
m)
Shoot
Root
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Abiotic Stress: some Trichoderma
strains and beneficial soil bacteria buffer plants from extreme environmental conditions:
• Waterlogging
• Drought
• pH extremes
• Nutritional stress
• Pathogen / Environment interaction
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Eco-T® buffers vs water-logging
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Effects of soil moisture in lettuce (inoculated with Pythium)
0
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700
800Y
ield
(g) w
et w
eigh
t
uninoculated controlinoculated control
biocontrol
Treatments
optimum irrigationhalf double
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0
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80
Mea
n w
et w
eigh
t (g
)
control(u) control(i) biocontrol
Treatments
6C
12C
18C
24C
30C
Effect of water temperature on biological interactions
in hydroponic lettuce (inoculated with Pythium)
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40
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160M
ean
tota
l wet
we
ight
s (g
)
control(u) control(i)
biocontrol
Treatments
pH4
pH5
pH6
pH7
Effect of pH on disease severity and biocontrol activity
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Formulations and Applications
• Crucial to activity – eg Bb
• Application must maximize BCA activity – e.g., Eco-T on seed vs drenching
• Needs to be simple, accurate, affordable to attract farmers
• Must not conflict with farmer operations
• New approach for field crops – Eco-T in John Deere talc+graphite lubricant for planter bins
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Biofertilizers • Symbiotic N fixation – only legumes
– Rhizobium nodules
– Promiscuous strains under development for other legumes
– Room storage at 200C
• Free Living N fixation – for all crops – Azotobacter, Azospirillum sp.
– Burkholderia sp., Klebsiella
• P access, dissolved from clay, rock phosphate – Bacillus spp.
– Trichoderma
– Mycorrhizae
• Micronutrient access (Mo, Bo, Zn, Cu, Fe) – Trichoderma
– Mycorrhizae
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Soybean –
Rhizobium nodules
(Strain WB74)
Red nodule =
fixing N
Green nodule =
parasite !
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Effect of microbial treatments on Soybean yield
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untre
ated
Eco
-T 1x
Eco
-T 2x
Rhiz
obium
Eco
-T +
Rhizo
bium
Treatments
Yie
ld (
g)
Series1
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Rhizobium vs waterlogging stress in wattle
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• N fixation for all the other crops
• Free-living diazotrophic bacteria to fix nitrogen in the root zone
• How much NPK fertilizer can be replaced by the best Azotobacter strains ?
• To date: 65% replacement of normal NPK fertilizer without affecting normal plant growth, using best strains
• P solubilization by other bacteria
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Figure Increased rooting in treated plants (left)
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Soluble Silicon in Plant Health • Silicon deficient soils - >70% of Africa short of soluble Si
• No deficiency symptoms but both physical and biochemical role in some plants (Si uptake is active)
• Grasses deposit Si as phytoliths into leaves; cane cuts
• Action vs Pests – accelerated resistance
• Action vs Diseases – accelerated SAR (immune system)
• Improves orderly cellulose deposition
• Stress tolerance – Cold, frost
– Heat
– Drought
– Al, Mn, Cd
• Synergistic with biocontrol agents
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Typical Opal Phytoliths (maize & grass)
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Liang, Sun, Zhu, Christie 2006
• Silicon effectively mitigates abiotic stresses:
– Manganese, aluminum and heavy metal toxicities
– Salinity
– Drought
– Chilling and freezing stresses
• Mechanisms poorly understood
Abiotic Stress Buffering by Si
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Cold Stress: bananas after 40C x 4 days
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The Future of BCAs
1. Fewer pesticides available
2. A wider range of BCAs
3. Better formulations, shelf-life, simplicity
4. Biofertilizers:
• N fixing – nodules and free-living
• Mobilization of P and micronutrients
• Buffering abiotic stress:
• Tolerance of heat, cold, drought, waterlogging, soil toxins, toxic metals
5. Soluble silicon - a powerful tool to add
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Potential Problems with BCA & BFZ
• Foreign strains, not well adapted to SA soils
• Many strains need to be screened to find one really powerful strain – requires time, money and research facilities
• Good BCA but poor formulation = ineffective
• Spores counts too low – 105 /g instead of 107 /g
• Contamination by other fungi
• It is not a chemical, but a living organism. Back seat of a car for 4 hours will kill it.
• Relatively short-lived except spore-formers (Bacillus), 6-12 months
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