NGS Bravo More Walk Away Time as Sample Numbers Increase · Walk Away Time as Sample Numbers...
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NGS Bravo – More Walk Away Time as Sample Numbers Increase Chad Whitman, Ph.D. Product Manager, NGS Automation February 4, 2016 1
Transcript of NGS Bravo More Walk Away Time as Sample Numbers Increase · Walk Away Time as Sample Numbers...
NGS Bravo – More Walk Away Time as Sample Numbers Increase
Chad Whitman, Ph.D.
Product Manager, NGS
Automation
February 4, 2016
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Agenda
Why Automate Your NGS Sample Prep?
Bravo NGS Platforms
Automation Benefits for NGS Sample Prep
Automated NGS Protocols for the Bravo Platform
Success Stories
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Why Automate Sample Preparation?
Automation will help with:
• Maintaining data quality with increased sample capacity
• Consistency - scales better with automation
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• Automation Engineers in medium
to large organizations, who support
many automated workflows for
entire groups
• Research Assistant looking for
turn key solution to address an
entire workflow
• Scientists looking for high quality
data from a complex workflow
Who can benefit from Automated Sample Prep?
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Key Benefits of Automated Sample Prep
Workflow Efficiency
Data Reproducibility Increased Yield
Throughput and
Walk-away time
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Agenda
Why Automate Your NGS Sample Prep?
Bravo NGS Platforms
Automation Benefits for NGS Sample Prep
Automated NGS Protocols for the Bravo Platform
Success Stories
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Agilent Automated NGS Sample Prep Solutions
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Agilent NGS Bravo
(Option A)
Part # G5541A
Higher throughput and improved
reproducibility on a small footprint
Agilent NGS Workstation
(Option B)
Part # G5522A
Agilent NGS Bravo +
Agilent BenchCel +
Agilent PlateLoc +
Agilent MiniHub
Higher throughput and improved
reproducibility with more walk away time
Add Agilent
PlateLoc for
efficient sealing
(Part #G5402A)
NGS Workstation (Option B)
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Bravo BenchCel 4R
Mini-Hub
PlateLoc
View of NGS Bravo Deck Setup
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CPAC CPAC Teleshake
Chiller Magnet
Agenda
Why Automate Your NGS Sample Prep?
Bravo NGS Platforms
Automation Benefits for NGS Sample Prep
Automated NGS Protocols for the Bravo Platform
Success Stories
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Automation reduces hands-on time with NGS Sample Prep
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Shear
Genomic DNA
Repair Ends
3’-dA Addition
Adapter
Ligation
PCR
Enrichment
Prepped
Library
SureSelect
Oligo Capture
Library Library
Hybridization
PCR
QA
Sequencing
SureSelectXT
Library Prep
SureSelect
Target
Enrichment
Automated
Bead Capture
Automated
Purification
Automated
Purification
Automated
Purification
Automated
Purification
Automated
Purification
Automated
Purification
Automated
Step
Legend
Off BRAVO
step
Sample Throughput and Hands-on Time Comparison with Agilent’s SureSelectXT on NGS Bravo Option B
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Hands-On Time for Manual Prep
Maximum Number of Samples
Processed/Day1
Library Prep 375 20
Pre-Capture PCR 90 20
Hybridization Preparation
60 20
Capture 210 20
Post-Capture PCR 90 20
Total Minutes 825 20 - 40 per week
Total Hours 13.75
Hands-On Time for Automated Prep
Maximum Number of Samples
Processed/Day
25² 96
13 96
7 96
20 96
40 96
105 192 per week
1.75
NGS Bravo Workstation drives 5x throughput with 7x less
hands-on time by automating pipetting and sample handling
Sample Throughput and Hands-on Time Comparison with Agilent’s SureSelectXT on NGS Bravo Option B
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Hands-On Time for Manual Prep
Maximum Number of Samples
Processed/Day1
Library Prep 375 20
Pre-Capture PCR 90 20
Hybridization Preparation
60 20
Capture 210 20
Post-Capture PCR 90 20
Total Minutes 825 20 - 40 per week
Total Hours 13.75
Hands-On Time for Automated Prep
Maximum Number of Samples
Processed/Day
25² 96
13 96
7 96
20 96
40 96
105 192 per week
1.75
NGS Bravo Workstation drives 5x throughput with 7x less
hands-on time by automating pipetting and sample handling
Example Workweek with Staggering Samples Using Agilent’s SureSelectXT
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Monday
Purify
45 min
Library Prep
4 hrs
PCR*
45 min
Purify
45 min
Quantify**
1.5 – 4 hours
Tuesday
Prepare Hybs 1.5 Hours
Purify
45 min
Library Prep
4 hours
PCR*
30 min
Purify
45 min
Quantify**
1.5 – 4 hours
Wednesday
Prepare Hybs
1.5 hours
Capture / Wash
3 hours
PCR*
45 min
Purify
45 min
Thursday
Capture / Wash
3 hours
Quantify**
1.5 – 4 hrs
PCR
45 min
Purify
45 min
Friday
Quantify**
1.5 – 4 hrs
Shear samples for following week
*Procedures take place off Bravo deck
**Quantification time for 96 samples: BioAnalyzer = 4 hours, TapeStation = 1.5 hours
Only Library Prep
Up to 2 plates/day = 960 samples/week
Agenda
Why Automate Your NGS Sample Prep?
Bravo NGS Platforms
Automation Benefits for NGS Sample Prep
Automated NGS Protocols for the Bravo Platform
Success Stories
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Available NGS Sample Prep Protocols – Agilent
Agilent NGS Reagent Sequencer User Guide
Publication #
Bravo
Platform
SureSelectXT Illumina G7550-900001 Option B
SureSelectXT SOLiD 5500 G7550-90002 Option B
SureSelectXT2 Illumina G9450-90000 Option B
SureSelectQXT Illumina G9682-90010 Option B
SureSelectXT RNA Target
Enrichment
Illumina G9691-90020 Option B
SureSelectXT Strand-Specific
RNA Library Prep
Illumina G9691-90030 Option B
HaloPlex Illumina G9900-90020 Option A
HaloPlex Ion Torrent G9900-90022 Option A
HaloPlexHS Illumina G9931-90010 Option A
HaloPlexHS Ion Torrent G9332-90010 Option A
Haloplex Exome Illumina G9906-90020 Option A
Validated, tested and fully supported protocols within VWorks Software
1 Supports low input (200ng) gDNA
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Available NGS Protocols – 3rd Party
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Company NGS Reagent Sequencer Bravo Platform
Illumina TruSeq RNA Library Prep Illumina Option A and B
TruSeq DNA Library Prep Illumina Option A and B
TruSeq Exome Enrichment Illumina Option B
TruSeq DNA PCR-Free Illumina Option B
TruSeq Stranded mRNA Illumina Option A and B
TruSeq Stranded Total RNA Illumina Option A and B
TruSeq Nano DNA Illumina Option A and B
TruSeq Prokayotic RNA Illumina Option A
Nextera XT DNA Illumina Option B
Roche/NimbleGen SeqCapEZ Library SR Illumina Option A and B
NuGen Ovation RNA-Seq System V2 Illumina Option A and B
Ovation Ultralow DR Multiplex Systems Illumina Option A
Kapa BioSystem HTP Library Preparation Kit Illumina Option B
HyperPrep Library Preparation Kit Illumina Option A and B
New England BioLabs NEBNext Singleplex Illumina Option A
NEBNext Multiplex Illumina Option A
NEBNext DNA Library Prep Illumina Option B
NEBNext Ultra DNA Library Prep Illumina Option B
Pacific Biosciences SMRTbell Library Preparation (10Kb) PacBio RS II Option B
Life Technologies Ion AmpliSeq Library Kit Ion Torrent Option A
Agenda
Why Automate Your NGS Sample Prep?
Bravo NGS Platforms
Automation Benefits for NGS Sample Prep
Automated NGS Protocols for the Bravo Platform
Success Stories
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TGen Success Story – From Bench to Bedside
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• TGen invested into a NGS Bravo
Workstation to address their
increasing throughput needs
• Transition from manual to
automated methods was “a quick
one” and was easy to implement
• Additionally the system provided
them with improved reproducibility
and data accuracy
NGS Bravo is part of Agilent’s complete workflow solution for Target Enrichment
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qPCR
SureCycler 8800
NGS Analysis
Sample QC
SureDesign
Automation
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Agilent Products are For Research Use Only
Not for use in diagnostic procedures
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Thank you for attending!!!
Questions???
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