BQ NONG NGHIEP CQNG HOA XA HQI CHtT NGHIA VIPT NAMVA PHAT TRIEN NONG THON DQc lip - Try do - H4nh phric

s6: 0? /2018/TT-BNNPTNT HdN,i,ngdyzlo thdng tndm20ly

THONG TI.I

Quy <linh vd t<i6m tra t4p ch6t trong t6m vh sin phAm t6m

Cdn c{r Ludr An todn rhLrc pham ngdy t7 rhdng 6 ndm 2010,'

Cdn ca LuQt Xa ly vi phqm hitnh chinh ngdy 20 thdng 6 ndm 2012;

Cdn c{r Nghi dinh s6 l5/2017/I,|E-CP ngay 17 thdng 02 ndm 2017 ctia Chinh

phil quy dinh chac ndng, nhiQm ur, qtyin han vh cct cdu td chac cila BQ N6ng

nghi(p vd Phtu niijn nong th6n;

Cdn cil'Quy6t dinh tii z+_tygn-ffg ngdy 13 thdng 12 ndm 2016 cila Tht)

tuhng Chinh phu phA duy\t Di an ki6m sodt ngdn chQn hdnh vi daa tgp chdt vdo

t6m nguyAn li€u vd siLn xudt, kinh doanh san phiLm t6m c6 tqp chrit;

Theo di nghi cila Cqlc tru'ong Cqtc Qtnn t!' Chih lu'qng N6ng l6m sdn vd

Thily sdn,

BQ trahng BQ N6ng nghi€p vd Phdt tri6n n6ng th6n ban hdnh Th6ng taquy dinh ri mA* ta tqp chdt trong t6m vd san ph6:m t6m,

Chuo'ng IQUY DINH CHUNG

Didu 1. Ph4m vi didu chinh

Th6ng tu ndy quy dinh vd ki6m tra t4p chdt trong t6m vd srin phAm t6m

(sau ddy vi6t chung tit t6m); tr6ch nhiQm, quy6n h4n cria cdc co quan nhir nu6c,.t , ,to chuc, cd nhAn c6 ii6n quan.

Didu 2. Diii tuqng 6p dgng

Th6ng tu ndy 6p dqng COi vOi tO chrlc, c6 nh6n (sau ddy vi6t chung ld Cct

so) c6 thuc hiQn ho4t dQng thu mua, b6o qu6':r, so ch6, ch6 bi6n, vQn chuy6n,

bu6n b6n t6m thuQc thArr quydn quAn lf cira BQ N6ng nghiQp vd Ph6t tri6n ndng

thOn, c6c co quan thArn quyAn vit c6c t6 chftc, c6 nh6n c6 1i6n quan'

Ei6u 3. Gi6i thich ti'ngir

Trong Th6ng tu ndy, c6rc tir, ngfr'du6i ddy duoc hi€u nhu sau:

1. Tqp chdt: ld c6c chAt kh6ng phai thdnh phdn tu nhi€n cia t6m.

2. Daa tap chdt vdo t6m ld ho4t dQng c6 f ngAm, tAm, uop, ntrOi nnet,

bom chich t4p ch6t viro t6m d6 lem tang khdi lu-o. ng, t6ng kich cd, ldm thay ddi

k6t cAu t}I'nhi6n crla t6m nhim mgc dich gian l{n thuong mei, k6 cA bi6u, t{ng,. ,1.rrao col.

3. Sdn xudt, kinh doanh t6m chrt'a ttp chcit ld vi6c thu mua, bao qu6n, sotl t:,.A ;. ,ch€, chO br€n, van chuyen, bu6n b6n t6m c6 tap chdt.

4. Vi pham vi tap chdt ld thuc liiQn rn6t trong c6c hdnh vi duoc n6u tai. ^ -.).Knoan z. Knoan J ljrcu nay.

Di6u 4. Ccr quan ki6m tra vA h)nh thf.c ki6m tra' ' 'l'l . Lo quan Ktem tfa

a) Co quan ki6m tra thu6c 86 N6ng nghiOp vd Phdt tri6n nong thon gdm:Thanh tra Bo Ndng nghiep vd Plrar tr i6n nong th6n, Cuc Quan ly chAr luongn6ng l6m sAn vd thiry s6r-r.

' .:. b) Co quan ki6m tra dia phuong g6ra: Thanh tra So NOng nghiQp vd Ph6ttri6n nong th6n, Chi cuc Quan ly ch6t Juong n6ng lAm san vA thuy sAn; cdc coquan chuy6n rrr6n kh6c theo phAn c6ng cua Sd Ndng nghi6p vd Phdt tri6n n6ngth6n.

2. Hinh thuc vd cdn c(r' l<iem tla:. ,..^a) I(i€m tra thuong xLryen ld ho4t clQng ki6m tra viQc chAp hdnh c6c quy

dinh v€ ki€rn so6t, ngdn chdn tqp chAt trong tom l<6t hqp ki6m tra, d6nh gi6 di6ukiQn b6o d6rr an todn thuc phAr.n viL ki6m tra, chimg nhAn 16 hdng xuAt khAu criac6c Co so theo quy dinh cira BO N0ng nghiOp vd Phdt tli6n ndng thdn vd ki6m., -.).tra, d6nh gid, di6Lr kien bao darn an toan thuc phAm vd ki6rn tra cdp chring thucho 16 hdng thiry s6n xuAt khau.

| |,.1b) Ki6m tra d6t xuAt Id hoat ddng ki€rn tra duoc thu'c hj6n l<hi c6 thong tinvi ph4m. Th6ng tin vi ph4m vA tap ch6t dr,ro-c thu thQp tri c6c ngu6n do t6 chric,c6 nhAn t6 gi5c, th6ng tin cua co- quan C0ng an, th6ng tin ve ket qua ki€rn so6ttap chAt.trong t6rn cira c6c co- quan ch&c nhng vd th6ng tin cAnh b6o cta nu6cnhQp kh6u.

Di6u 5. Y6u ciu ct6i vO{ phdng kiSm nghiQm!.;.

1. Phong ki6m nghiOrn c6Lc loai t4p chAt ph6i duoc Co qr-ran c6 thAm quy6nchi dinh c6c chi ti6u tuong iLng theo quy dinh ph6p h,r6t.

2. 'l'ruong ho-p chi ti6u cAn phAn tich chua c6 Phong ki6r.n nghiQm duo-c^ , -1chi dinh, Co quan ki€m tra xem xdt, lua chon gui mdu ph6n tich t4i c6rc Phdng

ki6m nghi€m d6p irng c6c y€u cAu sau:

a) Dd duoc chi dinh phdn tich c6c chi ti6u an toirn thuc phim.

b) Dd c6 phucrng ph6p vd x6c nhAn gi6 tri qu dqng phuong ph6p dOi v6i). ^cnl lleu can Dhan trch.

Chuo'ns IITRiNH TT'.

-KIEMTRA

Eidu 6. Thhnh l$p Eoirn ki€m tra, phdn c6ng ki6m tra' ^i.

.,1.\i . Dcjr voi kiern tra thudng xuydn:

' a) Co quan ki6m tra quy dintf tai kho6n 1 di€u 4 'fh6ng tu ndy rhanh hp'.,1Dodn ki€m tra, d6nh gi6 cti6u ki6n bao darn an todn rhuc phAm vd k€t hqp ki6m

tra viOc chdp hdnh c6c quy dinh v€ ki6rn so6t, ngdn chdn tgp chAt tai Co so.

b) Co quan ki6m tra quy ctinh tai cli6m a kho6n I di6u 4 Th6ng tr_r ndy cri'ki€rn tra vi6n ki6rn tla, I6y nrAu 16 hdng thriy sAn xufu khAu vA kdt ho.p l<i6m travi6c ch6p hdnh c6c quy dinh ud ki6m so6t, nghn chdn t4p chAt tai Co sd.

2. DOi vo'i ki6rn tra d6t xuAt: Co quan ki6m tra ban hdnh euy6t dinh rhdnhl4p dodn ki6rn tra d6t xuAt khi c6 th6ng tir.r vi pham vC tap ch6t. Trucrng hop cAn.t 't. ^ ,.;thr€t, Co quan kidrn tra thdnh lAp dodn ki6rn tr.a li6n ngdnh; moi dqi di6n cilc ccrquan, don vi kh6c thu6c ngdnh N6ng nghi€p vA Phdt tri6n n6ng thon, C6ng an,Cdng Thuong tham gia.

Di6u 7. Trinh tq ki6m tra tap ch6t t4i co s&

1. C6ng b6 Quyet dinh ki6m tra d6i vdi trudng ho.p thdnh l6p dodn ki€rn"t ^ r i ^ i, -'. ' ' ';tra: t)oan krem tl'a cong b0 Quy€t dinh ki€m tr.a cho clai di6n crlta co s6 khi ti6n

r . r r.l ) 'hanh klem tra. Ifuong hop co thong tin vi pharn v6 tap chAt datrg clrroc thuc hidnhoic tang v6t dang ducrc luu gifr t4i co so, Eo?rn ki6m tra thrrc hi6n ngay viQcki6m tra tpi ch6, sau d6 c6ng bO quytit clinh ki€m tra.

^ .,-; ,i , ,

, 2. Krem tra de phdt hiQn tap ch6t tr.ong tdm theo n6i dung,vd. phuong ph6pki6m tra quy dinh tai Ei€u 8 Th0ng tu ndy.

, a.3. I h6ng bd,o k6r qua: LAp thdnh bi6n bAn ldm vi€c vir th6ng b6o cho clai" ;.,.( ",.:adi€n co sd vd kdt quA ki6m tra. Truong h-o.p phdt hi€n vi pham v6 tap ch6t, Eodn

ki€rn tra hodc ki6rn tra vi6n trong trudng hop duoc phAn c6ng ki6m tra d6c l6p..4tren heinh lQp Bi€n bAu vi ph4rn hdurh chinh theo thAm quydn d6i v6i c6c hdnh vivi pham rheo rnau quy dinh tai Nghi dinh s6 9712017/ND-Cp ngriy lglgl2}ITcira Chinh phir sira dOi bO sung m6t sd di6u cua Nghi dinh 81/2013/ND-Cp ngdy191712013 cria Chinh phi quy dinh chi .ti6t mQt sO AidLr vd biQn phrip thi hdnhLuat Xft l1i vi pham hinh chinh.

Ei6u 8. NQi dung vh phuong phdp l<i6m tra t3p ch6t

i. N6i dung l<i6m tra:.A

. .a)Cac giAy to li€n quan d6n ho4t c16ng ctdng ky kinh doanh, chimg nhAndti di6u'ki6n b6o ddm an todn thuc phdm.

b) Vi6c qu6n lli nguyCn li€u nhap vd sir dung nguy6n iiQu dC s6n xudt kinhdoanh t6rn.

c) SO lu-ong s6n phAm dang dugc s6n xu6t vd kinh doanh, h6 so k! thuQt, ;1.(tieu chuan co so. ddng kli chAt luong s6n phAm).

ci) H6 so li6n quan d6n ho4t dQng tr,r ki6ni so6t cria co so.:' '.. id) Ki€m tra hi€n trang khu vqc sAn xu6t, kho nguy6n 1iQu, kho thdnh phAm

vd c6c khu vuc kh6c c6 li6n quan.

e) D6nh gi6 sr,t phir hqp quy dinh vd ki6m so6t tap chdt aOi vOi nguy€n,]li€u, brin thdrnh phArn vd thhnh phAm cua co sd.

g) C6c n6i dung l<h6c theo ydu cAu ughidp v9.

2. Phuong phdp lcicm tla:

a),Thu th6p tdLi 1iQu, chirng cf, th6ng tin theo nQi dung ki6rn tra, kdt hqpph6ng v6n vd thAm tra b6o c6o gihi trinh cria co sd khi cAn thi5t;

b) Lay mAu, l<idrn tla mdu tai ch6; lAy rn6u luu; ki6m tra tap chet tai phdngki6m nghiOm theo quy clinh t4i Di€u 9 fh6ng tu nDry.

DiAu 9. L6y m6u, ki6rn tra tai ch6 vi l6y m6u luu, ki6rn tra t4p cU6t t4i' .ipnong Klem ngnr9m

1. LAy mAu, ki6m tra t4i ch6: Do?rn ki6m tra hod.c ki6rn tra vi6n trongtrudng hqp ducrc phdn c6ng kiem tra cloc lqp chgn 16 t6m nghi ngd c6 tap ch6t

dd x6c ctinh ciuoc chu so hfi'u hodc ngudi chlr-r tr6ch nhiQrn dd ki6m tra t4p ch6t

theo Quy trinh k! thuAt ki6m tra t4i ch6 n6r-r tai PhU lUc 1 ban hdnh kdm theo

Th6ng tu ndy; iQp Bi6n b6n ki6m tra k! thu4t theo m5u t4i PhU luc 2 ban hAnh

kdm theo Th6ng tu ndy. Bi6n b6n ki6m tra l<! thuAt c6 cht k! ctatru&ng dodn, .l , .:.ki€m tra, ki6m tra vi€n vd cht ky cua clai dien c<r s6. Trudng ho-p dAi diQn Co sd

kh6ng kj bi6n b6n, bi6n bAn ph6i c6 chf ky cta nguoi chirng ki€n ho4c chir kycua tAt c6 c6c thdnh vi€n trons dodn.

^,i : ,z. Lay mau tu'u:

, --, , ia.; Khi kdt qu6 ki€m tra t4i ch6 ph6t hiQn 16 hang t6m c6 tap chat, Eodn

ki6m tra ho[c ki€m tra vi6n trong trudng'l-r-o. p dugc phdn cOng ki6m tra clQc lQp

thgc hiQn viQc l6y rnfru d6 Iuu tai Co quan ki6rn tla. Mdu duoc chia thdnh 03 don

vi nhu nhau, cluoc ni€rn phong c6 chir ky cua nguoi l6y mdu, dai di6n co sd

dugc l6y rn6u;

'. -'.^ 't x ' I xb) ViQc IAy mAu dugc lAp thdnh bi€n b6n vd phAi n€u 16 thdi han luu miiu

tai co quan ki6m tra phir ho.p v6i h4n sir clung cira sin phAm nhrmg khong qu6 15. ,.;. .! .. ,ngdy. H€t thdi han lrLu mdr-r md kh6ng c6 khi6u nai, co quan kiOm tra xir lf rrdu

luu theo quy dinh hiQn hdLnh;

c) Truong h<r. p d4i diqn Co sd kh6ng ky bi€n ban l6y m5u, ni6m phongi ,.^,,,,i; ; :

mAu, bi6n bdd l6y mAu c6n n€u rd l1i do "dgi diQn co sd kh6ng ky bi6n ban ldy

mAu, ni€m phong m6u". Bi6n bAn lAy m5u, ni€m phong m6u c6 chft ki cta

nguoi 15y m6u, Trudng dodrn ki6rn tra hoac ki6rr tra vi6n trong trudng h-q p dugc^ ' ':phAn c6ng ki6m tra dQc lap vAn co gid tri ph6p lj.3. ilrong trudng hgp khQng d6ng 1i v6i k6t quA ki6m tra tai ch6, trong

vong 01 Qn6t) ngdy kC tu ngdy ki€m tra, Co- so c6 quy6n dO nghi Dodn ki6m traho{c Co quan ki6m tra gui m5u luu d6n Phong ki6m nghiQm quy dfnh tai Ei6u 5

-ti ,^Th6ng tu ndy dd phAn tich. K€t qu6L ph6n tich cria Phdng ki€m nghiQm ldr cdn cfd6 co quan ki6m tra xri l! vi pharn v€ tap chAt. Chi phi phAn tich tai Phdng ki6mnghigm do Co- so chi trA.

Chu'ong IIIxrt LV Krrr PHA'I HIEN TA.P cHAr

Di6u 10. Xir tf khi ph6t hiQn t4p ch6t.,1 '

1. fluong hgp t6 chuc ki6m tla theo DoiLn, khi ph6t hi6n vi phqn vd t4p

ch6t ho4c k6t qud ki6m tra tai ch6 k5t lr,rQn 16 t6rn c6 tap chAt, Tluor.rg ctoAn ki6rn

tra thr-Lc hi0n:

a) Ldp bi6n b6n vi ph4m hdnh chinh theo thAm quydn.

b) Ni6n phong tang vQt, phuong ti6n vi ph4m hdnh chinh. Ni6m phong c6,i ,.1

ddu treo cfra Co quan ki6m tla, chf ky cria tru6ng dodn ki€m tra vd cht ky ctadai diqn Co sd. ViQc ni€m phor.rg phAi cl"ro. c 1Qp thdnh bi€a bfu. Trong trudngho.p kli6ng c6 chfr'ky cria clai diQn co so', ni€rn phong phdi c6 chfr k1i cua ngudi

t.7 tchung ki€n ho[c chfr kli cua tdt cA c6c thirnh vi€n trong clodLn vri ghi 16 trong bi6n

bin "dai diQn ccr sd kh6ng kf ni€rn phong, bi6n brln".

c) 86o c6o Thir truong co quan ki6rn tra dO ban hdnh quy6t Alnn t4m gittang vAt, phuong tiQn vi pharn trong thoi h4n kh6ng qua 24 gio k6 tir lchi ni6m

phong.

' .,i2. Truong ho.p ki6m tra vi€n dugc phAn cOng ki6m tra dQc lgp, khi ph6t

hi6n vi ph4m v€ t4p ch.6t hodc k5t qu6 ki6m tra t4i ch6 k6t iu4n 10 tOm c6 t4p, a ,.1cnal, Klem tra vten tnuc nlen:

a) LAp bi6n b6n vi ph4m hdLnh chinh theo thAm quydn.

b) 86o c6o Thrl truong co qr-ran l<i6m tra d6 c6 biqn ph6p xir lf phir hqp.

EiAu 11. Xii lj, b)n giao vg viQc vi phgm hhnh chfnh1.;ll. Tru6ng dodrn ki€m tra hobc ki6rn tra vi€n trong trudng ho. p dugc phdn

c6ng ki6m tra tl6c lQp b6o c6o Thrl trudng co- quan k16m tra hodLn thiQn h6 scr xirlli vi pharn hAnh chinh theo thArn quydn.

2.'Trong tluo-ng ho.p clodrn ki6rn tra cita co- quan ki€rn tra l\ung uong ph6i

di chuy€n ngay d6n dia dieirn kh6c d6 ki6m tra, xfr l! vi ph4m vd tap ch6t, b6o

dAm tinh kfp thdi, bdro mdt, Truo'ng doiLn ki6m tra b6o c6o, xin 1i ki6n Thri

trudng co quan ki6rn tra d6 bdn giao vu vi6c cho c6rc co quan dia phuo"ng xir lf.Vi6c brin giao vu viQc dlro. c tliqc hiQn nhu'sau:

a) Co quan tidp nhqn bdn giao ld Thanh tla So Nong nghidp vd Phdt trien

ndng thon hodc Co quarr duoc giao nhiOrn vLr thanh [r'a clruy6n ngdnh tai diapnuong.

b) Vi6c bdLn giao phai clucrc lAp bi6n brin vd c6 chir ky cira Trudng dodn,.ilcrenr tla vdr dar di6n b€n nhdn birn siao.

ltu'o'ng rvTRAC{-I NHIEM VA QUYEN I{AN

Di6u 12. Thirnh vi6n clohn ki6m tra vh ki6m tra vi6n tluo'c phAn c6ng

ki6m tra tl6c l6p

l. Trdch nhifrn cua thdnh vien doAn kidrn tla vA lcidrn tla viin duoc phAn

c6ng ki€rn tra d6c l6p:

a) TuAn thir dirng qr"ry clinh cira Co s6 vd vQ sinh c6 nhAn tru6c khi vdo Cos6;

b) TuAn thir di.rng trinh tU, thu tuc, n6i clr-rng, phuong phap ki6rn tra tap ch6t

theo quy dinh tai Thdng tr-L ndy;

c) 86o mAt c6c th6ng tin li€n qLran d6n s6n xuAt, kinh doanh cua Co sd theo

quy dinh cia ph6p lu$t; d6rr bio c6ng khai, rninh bach, trung thuc, kh6ch quan

vd t<hdng phdn biQt OOi xu fni thlrc hiQn nhiern vu;

d) Kh6ng yOu cdu th€m cdc n6i dung lch6c ngoiri qr-ry clinh g6y s6ch nhi5u,l.:

Dnlen na cno LCt so:

.,^'id) Chap hiurh sr,r phAn c6ng cira'fruong dodLn ki€m tra viL Thir tluong Co quan

ki€rn tla; chiu tr6ch rrhiOm vd k5t qu6 ki6m tra tru6c Thu tru6ng Co quan ki6m tra,,A +-, "X^ ^l^i^ l,,A+.vcr Lr Lr(J! Plro'lJ ruaL,

e) ChuAn bi dAy dn trang thi5t b!, dL.rng cu, dung dich thir, tdi li€u kl thuQt

phuc vu cho ho4t dQng ki€nr tra;. ,..:.g) I(€m tra tinh trang rndu 1uu vdr rnd h6a mAu (c6 ky hiQu nhQn bi6t) tru6c

khi g0'i m6u d6n Phdng ki6m nghiQm cltl kitim nghiQm khi c6 y€u cdu.).,

2. Quy6n han cira thdnh vi€n dodn ki6m tra vd ki€m tra vi€n duo. c ph6n c6ng

ki0m tra dQc lQp:

:^a) Y€u cdu Co sd cung cdp h6 so, tdi 1i0u, mdu vQt vd cir nhdn vi6n Lim vi6c

nhirn ph\rc vu eho cOng t6c ki6m tra;

b) Ra vrio c6c khu vuc san xuAt, khLr ti6p nhAn, b6o quin nguy6n li6u vd c6ckhu vqc phu tro kh6c khi c6 nhAn vi6n co- sd di cing hodc dd b6o v6i nhAn vi6ncria ccr sd;

, ':c) Xem xdt hd so, lAy mAu, chup Anh, sao ch6p, ghi ch6p c6c th6ng tin cAnthi6L d6 phuc vu,cho nhient vu ki6rn tra.

Ei6u 13.Tnr6'ng tloiin ki0rn tra

1. Tr6ch nhi6m cua Truong do2ur ki6rn h'a:

a) C6 trach nhi6m rheo quy dinh tai khoan 1 Ei€r"r 12 fh6ng tu ndy;

' ' ^.:b) Dieu hrinh, phrin cdng nhi6m vu cho c6c thdnh vi6n trong clodn ki€rn tradd thuc hi€n dAy du c6c n6i clung tlong quy€r dinh thdnh lrip dodn ki6rn rra;

c) Xri Iy c6c y ki6n. k6t qud ki6rn tla cua c6c thanh vi6n trong doAn ki€m travA dua ra kdt luan.r6i cung tai bidn ban kienr tra;

d) Mdi clai didn hodc nhAn vi€n co su: tham du cdng bO quyat dinh dodn'.,ilci6in tra vA di cDng doAn l<iern Lra;

d) Rd so6t, kli bi6n bdn l<i6rn tla, chiu tr6ch nhi6m tn-L6c th,.i trr,r6ng Co quanf .ikrem tra va truoc ph6p ludt v€ kOt quA d5 duo-c dodn ki6rn tra thuc hi€n.

2. Quy6n han crla Tluolg clodn ki6rn tla:

a) C6 tr6ch nhi€m theo qr-ry ctinh t4i khodn 2 Ei6u 12 Th6ng tu ndy;r.ni A, " -,, i, ,. , -.tb) De xudt vdi Thi truorig Co quan kiOm tra ban hdnh quydt dinh di€u chinh

thanh vi6n doan ki€m tla d€ thqc hiQn dAy dir c6c nQi dung h-ong quy6t dinh thanh- . . .i

rap ooan Kem u'a;

c) Dua ,u tet tran cudi cirng cua clodrn ki0m tra vO k6t qr,r6 ki€m tra.

Di6u 14. Co'quan ki0rn tra, ,ll. Xay dung l<€ hoach h2rng ndm kdm theo dr_r trir kinh phi; tdnh cAp c6 th6rn

cluy6n ph6 duy€t.

2. T6 chirc ki6m tra t4p ch6t theo quy dinh tai Th6ng tu ndy.

3. Thuc hi6n xt' lj vi pham hdnh chinh theo thAm quy6n hodc ph6i ho.. p v6i, ,7 : ,,'

co quan co thdm quy6n dO xu lj vi pham hanh chinh theo quy dinh;

4. Luu trt dAy dir hO so'c6 li€n quan d6n k6t quA l<i€rn tla, b6o cdo diy dt vdchinh x6c vd c6c vAn dC liCn quan cl6n hoat d6hg ki6m trat4p chdtkhi C<r quanco rnant quyen yeu cau;

!. BAo rndt th6ng tin liOn quan cl6n bi iir4t s6n xu6t, kinh cloanh cira c6c Coso dLLo-c kidm tra tqp ch6t.

Di6u 15. 56'Ndng nghipp vir Ph6t tri6n N6ng th6n

' -: , i | '^1. T6 chuc ph6 bi€n, tuy6n truydn cho c6c c16i tugng c6 1i6n quan: chri co sd

nu6i, co sd thu mu4 so ch6, dai lf thu mua, co sd chii bir5n thfiy s6n vA t6c hpi1 ;.cfia tap chet vd cdc quy dinh cia Nhd nu6c vd ki6m tra, ngdn ch[n vi ph4m vO

tap chAt;

2. T6 chric ddo tao v6 phucrng ph6p ltidrn tla, ph6t hiQn t4p chAt trong thriy

s6n cho c6c cdnbQ ldm nhiQrn vr-.r ki6rn tra t4p ch6t cAp huyQn, cdp xd.;

, ,,(, l3. XAy dpg k6 ho4ch ki6rn tra d6t xuAt, ngdn ch{n vi pham v0 t4p chAt. 86

, , ,1 ,tri dir kinh phi d€ phuc vu cho c6c hopt dQng ki€m tla;

4. Ph6i h-o.p v6'i c6c co quan chirc n6ng c6 li6rr quan, cdc t6 chuc dodn th6,,).

hQi ngh6 nghiQp t4i dia phr-rong tO chuc c6c hoat dQng ph6 bi€n tuy6n truy€n v€

t6c h4i cira t4p ch6t, quy dinh vC kiCm tra, ngdn cl-r{n vd xir 1y vi pham vd t4p

cnat;

5. Chi d4o Thanh tra 36, Chi c1rc Qu6n li Chat lugng n6ng 1Am s6n vd thiry, i . ,.is6n sdn sring ti€p nhAn vu viQc do clo2Lr.r kiem tt'a cria Co quan ki€m tra trung

uong bdn giao vd xu' l! theo quy dlnh cua ph6p luQt;

6. Ri6ns Sir N6ns nghidp v2r Plidt tri6n nong th6n C2r Mau, B4c Li6u, S6c"o ";r -

Trdng, Ki6n Giang chi d4o thanh 1dp duong ddy n6ng dC ti6p nhQn c6c th6ng tint6 crio vi ph4m t4p chat; tap danh s6ch c6c co'sd thu mua, so ch6 vd t6 chirc kicam k6t kh6ng dua tap chAt vdo t6rn vd kh6ng sdn xudt kinh doanh torn c6 tap

chAt.

Di6u 16. Cqc Quin lf ch6t lu'o;ng ndng lf,m sin vir thfry s6n

1. Thr,rc hien ddy dt tr6ch nhiQrn cria co quan ki6m tra n6u tpi Di€u 14

Th6ng tu ndy;

2. 'fhuc hiqn ddo t4o k! thuAt ph6t hiQn t4p ch6t cho c6c l<i6m tra vi6n cdp\^

t l'ung uong va cap lrnn;

3.Hdng ndm, chu tri vd ph6i ho.p v6i c6c Co quan ki6m tra trung uorlg,.A, , .A

xAy dqng k€ hopch tri€n khai c6c ho4t dQng li6n quan cl€n ki€m-so6t t4p chdt

trong tdm kdm theo du trir kinh phi trinh BQ N6ng nghidp vd Phdt tri6n n6ngil"A- ^hA;,,.,4r'

4. Hu6ng d6n th6ng nhdt c6c co quan qudn 1i chAt iugng n6ng lAm thfiy, a ). ^ ' '1.t :.'

san tinh, thdnh phd vd n6i dung tuy€n tluy6n, ph6 bi€n v6 tdc h4i cua tap chdt vd

c6c quy dinh cria Nhd nu6c vA ki6m tra, ngdn chdn vi ph4rn v0 t4p chdt;

- -; , (. .: |

^. ri

^, .1'

5. TO chi.rc so k€t, t6ng l<6t, dC xudt giAi phdp thdo gd lch6 khdn vu6ngJ , 1,'mdc vd khen thuong dQng vi6n c6c tO chirc, c6 nhdn 1dm t6t trong c6ng tdc ki6m

tra ph6t hi€n vi,pham vO t4p chAt.

Di6u 17. Phdng ki6m nghiQma t..^l. Ki€m tra tinh trang rr.rAu, tinh trang ni6m phong rnAu, l{p bi6n bin ti€p

nhAn vd th6 hi6n tinh rrarrg rnAu. tinh tr4ng ni6m phong mAu tr'6n k6t quA ki6mnghi6nr;

.i2. Ddrn bdo J<6t qua l<i6m nghiQrn'chinh x6c, klp thoi, k_trdch quan, trung thUc

vd chiu tr6ch nhiQm v6 k6t qua ki€rn nghiQm;

3. Luu trfr h0 scy. tdi lieu li6n quan d6n hoat dong ki6rn nghi€m tap chAt dfngquy dinh vd xuAt trinh khi Co quan thArn quydn y6u cAu.

Chu'ong VIIDIIU KHOAN.TI{I HANH

Di6u 18. HiQu h1'c thi hinh^t'fh6ng tu nay co hi6Lr lpc k0 tu ngityZlthtng I ndm 2018.

Noi nhQn:- Thir tuting Chinh phLu- Vnn phdng Chinh phir:- 86 'Iu ph6p:- C6c Bd: Y t6. 'l'di chinh. Cdng'l'hLrong. C6ng an;- U.BND, Sd N6ng nghiep vd PTNT cric tinh, thdnhph6 tluc thu6c Trung uo-t1g;- LanD oao tJo:- C6c rlorr vi thu6c 86 N6ng nglriep vd P-|NT:- Website Chinh phir; C ong b6o Chinh phu;- Website BQ N6ng nghi€p vi P'fN'l':- Ltru: V'l', Ql-Ct-.

K'r. Bg rRrloNG

Phu lgc 1

(ban himh kdm theo Th6ng ttr s6 07 nOlAtff-AwNpTNT ngdyz/p thang V ndm 20lB

cia 86 tru'6lng BQ N6ng nghiQp vd Phdt ffi6n n6ng th6n)

QUY TRiNH KV THUAT KTENI TRA T4.I CflO

I. PHAM VI AP DUN(;

Quy trinh ndy hu6ng clln viQc ki6m tra, d6nh gi6 vd ph6t hiQn c6c lo4i t?p chAt:

tinh b6; lva leotyuinyl ilcohol), CMC (Carboxymethyl cellulose)' Adao-(Gelatine)

v2r Agartrong t6rn nguyen liQu u6p d6, t6rn b6n thdnh phArn u6p d6 vir tOrn d6ng lanh'

II. GIAI THiCH TT' NG{I

Trong quy trinh nay, c6c tir ngt du6i ddy duoc hi6u nhu sau:

1. Ddnh gi6 chmquan: liL vi€c sir clung gi6c quan (nhin' ngiri' n6r.r.r' sd ndn vd nghe)

d0 x6t do6n, d6nh gi6 nhirng tiOu chi 1i6n quan ddn chdt luong'

2. L6y mau c6 chgn lga: ld vi€c l5y rn6u d4i cli6n trong nhiing 16 t6rn c6 dAu hiQu

c6 t3p ch6t.

III. CHUAN BI KIEMTRA

1. Cdn b0 ki6m tra ph6i chuAn bi tai,liQu k! thuf,t c6 li6n quan' bi6u rn6u'.nhdn mdu'

tlang pl.ruc v2r c6c dpng "\, fh\-r. v1r ki6m tra,'l6y m6u vi b6o quin mAr-r phn hqp'

2. Dpng cq, phuong tiQn kiOm tra c6m quan

- Dao inox nh6;

ETa Petri hodc lame kinh;

- Thia inox mi€ng nh6;'...- Kinh lfP;

- Cdn treo:

- Trii nhUa PE;

- l hd nhdn kh6ng thArn Iruoc;

- Birt ldng ddu hoac b0t bi kh6ng thArn'nrroc;

- M6Y Anh k! thu4t s6.

3. Dtlng cu, ho6 ch6t ki6ni tra nhanh bing phuo"ng ph6p hod hoc

3.1 ChuAn bi t4i Phong thi nghiQm:

- Can k! thuQt, d0 chinh x5c 0,01 g;

- Mey xay rndul

- Ong nghiQrn thiy tinh, 6ng facol 50rnl c6 ndp;'

;.- uen con;

- Binh dinh mirc 100rrl. 1000m1;

- Bt! di6u.nhiQt;

- r 'Pvr

Jrur,

- Dia petri;

- Ong dong 100m1, 1000rnl;

- C6c thu! tinh l00rnl, 250m1;

- Ho6 chAt loai tinh ]<hi6t phAn tich

C KI;

. Iod;

" Nu6c c6t;

C H,BO:;

o Acid tannic;

" CuSO, ho6c C,SOq sH'O;

o NaOII;

- Na,CO:;

o CeHsOrNa:;

C HC1;

e FIzSO4l

c Axit Chrornotropic

3.2 Dung dich thu6c thri t4p ch6t

a) Dung dich thudc thri tir.rh bQt:

- Cdn chinh x6c lan lurvt 2,0g tinh th6 Iod vd 6,09 tinh th6 KI vao trong cirng mQt

c6c thuj'tinh 100m1. Th€m vdo cOc 100m1 nudc c6t. KhuAy d0u bdng dfra thtytinh tl€n khi Iod vd KI hoa tan hodn todn.

- Thdi h4n b6o qudn dung dich trong t6i 6 nhiQt dQ phong: khdng quri 06 th6ng.

b) DLrng dich thu6c thu PVA:

- ChuAn bi dung dich locl (dung dich A):

o CAn chinh xdc iAn luqt l,27gtinhth6 Iod ya 2,509 tinh th6.I(I vdo trong

cirng m6t cdc thty tinh 100m1. Thdrn vdo c6c 100m1 nudc cAt. Khudy d6u

Oing Oua thriy tinh d6n khi lod vd KI hda tan hodn todn.

. Thd'i h4n bAo qu6.n dung dich trong t6i 6 nhiet dQ phdng: khong qud 06

th6ng.

- Chudn bi dung dich H:BO: 4"/o (dung dich B):

c Cdn chinh x6c 4,0g tinh thC HrBOr virb ttong c6c thriy tinh. 100rnl' Th6rn

vdo c6c 100m1 nuoc cAt. Khudy el6u bang dfra thriy tinh d€n khi hda tan

hodn todn.

' o '11661 han bAo quin dung dlch o nhidt dQ phong: khdng qu6 o th6ng.

- Pha dung dich thu6c thu PVA:

e Trudc lihi su dung. chuAn bi clung clich thLr6c thri PVA bing cdch trdn Geu,4//

.2 ,./4K--

hai dung dich A vd B theo ti lQ A:B : I :3 '

. Thoi ha' b6o c1u6n clung dich trong t6i 6 nhiCt dQ phdng: kh6ng qud 3 ngdy

c) Dung dlch thu6c thu Agar:

_ can lan lugt 2.0g tinh th6 iod vd 6.09 tinh th6 Ki, hda tan vd dlnh mirc ddn

l00rnl bing ntfoc cnt

- Thdi hqn bAo quan dtrng dich d nhidt dQ phong: kh6ng qu6 c thdng'

cl) Dung clicb thu6c thir Gelatine:

- CAn 5s tinh the acid tar.rnic vdo trong cdc thriy tinh 100rnl. 't-hdm v2ro c6c 100m1

nuOc JAt. I(hLr6y d6u blng dha thiy ti'h d€n khi acid tannic tan hoirn todn.

- Thoi han bao quan clung dich o nhi€t dQ phdng: kh6ng qu6 o thdng'

e) Dung dicli thu6c thir CMC:

Dung clich thu6c thtr Axit chrolnotropic 5o%: cdn 0,5g Axit chron-rotropic tr€n

"an [y inuat (d:0,0Ig1 viro c6c thiry tinh lO0 ml, cho thcm tir tir 10m1 Axit

sulfrrricg8%,khudytandeubangduathirytinh.BAoqrr6n6nhi€tdQphdng'dtrrrg dich sLr clung trorrg ngny

t) Dung dich thLrdc thiL agar trong thit t6m:

- Dung dich thu6c thu Benedict: Cdn I7 '3g CeHsOuNa: vd 10g NazCO: hda tan

tronf g0ml nu6c n6ng. cdn 1.?3g CuSOa.5FIzO hda ran trong.l0ml nu6c. Loc

dich, trOn 2 clung dich tr6n vd dinh miic d6n l00ml bing nudc cAt'

3.3 Dung dich ki6rr tra:

' - LA c6c dung clich tinh b6t, PVA, agar, gelatin va !fU! duqc chuAn b! o n6ng dQ

gi6i h4n phdrt hiQn Oe ti6ni tra hi6u ndng cira dung dich thu6c thu'

.a)DungdichtinhbOt0,03%:Cdlr.chinhxdc0,309tirrhbQtchov2Lo1000rnlnu6'ctAt. bun n6ng d 80'C, khuAy d6u cho tan ho2rn to?rn'

b)DungdlchPVA0,03Vo:Cdnchinhx6c0,309PVAchovdo1000mlnu6ccAt'KhudY d€u cho tan hodn todn.

c) Dung dfch agar 0,1%: CAn chinh x6c 0,10 g agar cho viuo 100 nrj nucrc cA1' Eun

nhg, khuAY ddu cho tan hodn toan'

cl) Dung dich agar' 0,2o/o: Cdn chir.rh x6c 0,209 agat cho vdo l00 ml nu6c cdt' Dun

nhq, khuAy d6u cho tan hodn todn'

e)Dnngdichgelatin0,Io/o:Cdnchinhx6c0,l0ggelatinechovdol00n.rlnu6c.At. bun n6ng 6 80"C' khu6y ddu cho tan hoiLn to2rn'

f.; Dung dich CMC 0,2V0: Cdrtchinh x6c 0,209 CMC cl.to vdo 100 ml nu6c cAt'

Eurin6ng 0 80'C, khtiAy cfdu cho tan hoirn todn'

3.4 Ki6rn tra hiQu ndng sir drlng cria c6c dung dich thu6c thir t4p chdt:

Tru6c hhi eli hion truong c6n phdi ki5rn tra hiQu ndng sir dung cria c6c.dung dlch

tf-'ro. tlJAa "ft"a" frl d mul 3.2 ting cach nh6 01 giqt dung dich thr-r6c thir vdo 0i

;i;;;;; Ji"n n6n.' tra dd chu6n bi6lmr-rc 3'3 tuo'g irng' Quan sdt vdddnhsid.hiQtt

iang "ui

dung.dich thu6c thir t4p ch6t rhO'rg qua sq bi6n ddi trzlng th6i cira thu6c

thir/dung dicl't : //,,-) 7

zzl_

hai dung dich A vd B theo ti l0 A:B = 1:3'

. Thdi han b6o quin dung dich trong t6i 0 nhiet dQ phdng: kh6ng qu6 3 ngay.

c) Dung dich thu6c thiL Agar:

- Cdn ldn luqt 2.0g ti.h th6 Iod vd 6.09 tinh th6 KI, hda tan vir di'h mric den

l00rnl bing ntL6c c6t.

- Thdi han bAo qudn dung ctich 6 nliiqt d6 phdr.rg: kh6ng qu6 o thdng'

cl) Dung dich thu6c thiL Gelatine:

- cdn 5s tinh thc acid tar.rnic vdo trong c6c thiry tinh lootnl. -L-h6m vAo c6c 100m1

nuOc c6t. Kh,Ay ddu bdng dua thuy tinh c16n khi acid tannic tan ho2rn toAn.

- Thoi han bdo quan clung dich o nhiet d6 phdng: kh6ng qu6 e thdng'

e) Dung dich thudc thiL CMC:

Dung dich thu6c thir Axit chromotropic 5%: cdn 0,5g Axit chro'rotropic trdn

"an [y ifruat (d:0,0ig; v]ro c6c thuy tinh 100 ml, cho then] tt tt 10ml Axit

sulfuric gg%, khu6y tafl dau bing dna thuy tinh. Bio qu6n d nhiQt d6 phdng,

dLrng dich srL tlung ttorlg ngay.

t) Dung dich thu6c thfi agar tfong thit t6n1:

-Dungdlchthu6cthirBenedict:CAr-r17.39CeHsorNa:vdl0gNazCo:hdatantrong 80rn1 nu6c n6ng. cdn 1.73g CuSO+.5HzO hda tan trong.lOml ltu6c. Loc

dich, trOn 2 clung dictitrdn vd dinh mirc d6n 100m1 bing nudc cAt'

3.3 Dung dich kidrn tra:

- Ld c6c d'ng clich tinh bor, PVA, agar, gelatin vd CMC drLoc chuAn bi d n6ng dQ

gi6i hqrn ph6t hi€n d6 tiern tra hiQu ndng cita dung dich thudc thu'

.a)DungdichtinhbOt0,03%:CAn.chinhxdc0,309tinhbotchovdo1000mlnu6c' "at.

b,.tn n6ng d 80'C, khu6y cliiu cho tan hodn todn'

b) Dung dich PVA 0,03ok: C|tnchinh x6c 0,309 PVA cho v?ro 1000 ml nr.r6c cdt'

KhuAy dOu cho tan lioiLr-r toirn.

c) Dtrng ciichagar 0,lo/o: c2tnchinh x6c 0,10 g agar cho vito 100 rrli nuoc cdt. Dun

nhe, lchudY d6u cho tzrn fodn toan.

d) Dung dich agar 0,2oh: cdnchinh x6c 0,209 agar cho viro 100 rnl nurvc cat. Dun

nhe, khudY d6u cho tan hodn todn.

e)Dirrrgdichgelatin0,|o/o:Cdttchinhx6c0,l0ggelatinechovilol00n-rlnu6c.6t. bun n6ng cr 80"C, khu6y dOu cho tan hodn toirn'

f) Dung dlch CMC 0'2Vo: C6n chinh x6c 0,209 CMC clro vdo 100 ml ntr6c c6t.

Eun n6ng d 80'C, khuAy d6u cho tan hodn toiLn'

3.4 Ki€rn tra hiQu ndng sri clqng cria c6c dung dich thu6c thri tqp ch6t:

Tru6c khi di hidn trucrng c6n phdi ki6m tra hiQu n6ng.sir dpng cira c6c.dung dich

thu6c thir da ChuAn bi 6 rnUi 3.2 ting c6ch nh6 01 giqt dung dich thu6c thir vdo 01

giqt,lu,.,g dich ki6m tra dd chu6n bi d mgc 3.3 tuor.rg irng. Quan s6t vd d6nhgi6.hiQn

iang .ua dung dich thu6c thir t4p ch6t thO'g qua sq bi6n et6i tr4ng th6i cira thu6c

tht/dung dich : /1

Ar/4a-

Dung dich thudc thii'Dung dich kiSm Su'bi6n d6i al4c

tru ng

IIiOu ndng criadung d,ichthu0e thf'

Dung dich thu6c thiLtinh b6t

Dung dich tinhb}t 0,03Yo

E6i mdu tir d6 sangxanh den

T6t

Dung dich thu6c thriPVA

Dr"rng dich P,VA0,03%

Edi mdu tir vdng cl6

sang xanhr6t

Dung dich thudc th[rAgar

Dung dich Agar0.r%

Edi rndu tt nAn d6sang tim clen

r6t

Dung dich thu6c thftAdao

Dung djchgelatin 0, 1%

^i.iJor lrau ttr vang nnalsang k6t tta trdng

r6t

dich thu6c thitDungCMC

Dung dich CMC0,2%

Dung dich thu6cc6 mdu tin-r taiphdn cAch

thirlop T0t

Dung dichAsar irnnq(Bennedict

thu6c thiLthit t6m

Dung dich Agar0,2%

D6i mdu tiL xanh sangxanh ld, kOt tira ai6

gach ddc trungT6t

3.5 ChuAn bi thu6c thu t4p chAt ki6m tra tai ch6:

- R6t tiLng loai clung cljch thu6c thir tinh bQt, PVA, agar, gelatin vd CMC dd duocchuAn bi 6 muc 3.2 vd ki6n tra hi6u ndng sir clr"tng d muc 3.4 vdLo tirng chai nh6 gigt5ml;

- D6n nhdn nh4n diQr.r tung loai thu6c thu; Dung dich duoc bao qu6n d nhiQt d0phong vd chi sri dung trong ng2ry.

rv. x^c DNH L0'r6m KlBnn Tna

l. ifi6m tra hd so, thu thf,p th6ng tin el6 xfc dinh 16 t6m:' ,;lfti phdLt l.riQn c6 dAu hi0Lr nghi ngo t6m c6 chfia tap chdt, nhdn vi6n ki6rn tra

x6c dinh chri s0 hiru, khdi luo. ng 16 t6m, chirng 1o4i hdng h6a, sau ct6 c6 lflp l0 t6m...( , . , ,^ x ,.;ti6n hirnh l6y rndu ki6rn tra tap chAt.

2. Nguy6n tic l6y rniu ki6rn trn:

- Ap dqrng ngtry€n tii Ii1 rnirr c6 clron lua nham dat clucrc khi ndng phAt hiencao nhAt sq hign di6n cLra.tap chAt tron! t6m.

- Can c(r d6 chqn rnAu l2r nhlrng bldu hiQn bit thud-ng vd tinh tlang bdn ngoiri cua

t6m (kich c6, hinh dang, lthi€m hhuyCt vflt mdu sdc (biOn mdu, mdu la), mti (bi€n mii,.. ' ' ,,(' :rnui 14), k€t cau (dai, bo, nhfrn,...), c6rr nh6n xric giric (cring, m€m, tron, nhcrt,

nhdy,...)..^ ,.1.

- NhAn vidn kiem tla ap dr-rng cdc k! r.idng vd phucrng ph6p cdm quan clO nhfln bi6tnhiing bi6u hien bat thuo'ng tltn urdu vflt ki6m tra dC dAnh gi6 vd xdc ctinh c6 tap ch6t

x^,'ong r-r1au val nay Knong.

- Ifti phrit hi€n hodc nghi ngd t6m c6 tap ch6t, nhdn vi6n ki6m tra st oungphuong plr6p thir nhanh h6a hoc n6u tai Muc VI dO x6c dinh loai t4p ch6t c6 trongtOrn.

. - -l-rudng hr2p sau 1<hi su ciung phuong_ phhp thir nhanh h6a hqc tqi ch6 vhn chua

kOt lufln clugc kdt qui chinh xac, cdn ldy ndu giri phdn tich taiphdng thit nghiQm.

a'ry'///ZA//<=.--

3. I-6y miu

3.1 D6i vdi t6m t'uoi u6'P clf :

- L6y mAu dai cli6n: rn6i.nre hirng c6 dAu hiQu nghi^ngd c6 tap ctr6t.tAy ir nhAt 0l

rnSu v6i ti tC t-Sy" so vdi khdi luqng m6 hdng. C6c mfiu sau khi thu thdp dugc t6p

truns lai ue ttdn dd, voi nhau thdnh mAu dai di6n'.' ""' ':'i;;;';; '#" ;;i;'c" nt.,ir'.'g tnan i6rn bi nghi ngd c6 tap ch6t dc kic'r tra c6m

quan x6c dinh t4rp ch6t iheo trinh tu vd thao tdc n6u t4i Muc V'

3.2 Ddi v6i tdm d6ng l4nh:

f.iO" tar xu6t (c6 .ini .a" yc, t6 t'c dQng d6n elii;, kign b6o dnm ATTP) trong thdi

gian kh6ng qtt6.24 gid tai n.r6t Co so'.

n: s6 mAu ldy ki6n-r tra; c: sd ttrau l<tett.l tl'a,crlo phep lclollg o?r' . . )

(-) LO hdng san xu6t: ld rnQt.luong sAn phdnr dLro. c sAt'r xuAt tir mdt hay nhi6u 16

nguy6n li6u c6 cing t.tguo,.t ga. *uat xt in"o cnng mQt quy.trinh. c6ne 1$11' :'"C g:tl

V. KIEM TRA CAM QUAN

ViQc ki€rn rra, c1d'h giA v2r phat hi0. tqp chAt trorrg t6rn bing pltrcrlg P5dp c?Lrn

quu,', pirai dugc titin hdnhlheo trinh ttr tiL t6ng qtr6t d6n chi tiet, t$ gf an 0a1t xudng dOn

p1rAn b6t du6i vd tiL ngodi vdo trong con t6m. Cdc bu6c tl.r*c hiQn ki6m tra bao g6m:

-TT I-.1,2.

- Lo d;g ki6m tr"a

Lo hd"s kffi t* b^"Gb*, i Oiln : 16 hdng sin xuAt(")

a,, - --:- -- -:r Lo hdng kiOm tra bao gdrn: >3 16 hang sin xuat

-56 ','tEu

tay ki@lgn:6, c:0

-n:g 6[n n=13, c:0

n 6 *il16v t i6m tra; c: s6 n.r6u ki6tr.r tra cho ph6p kh6ng d4t'

MAU KIEM TRA

KIEM TRA TiNH TRANG BEN NGOAI

KIEM TRA SAU KHI BOC VO DAUI.IC

KIEM TRA CO THJT DA BOC VO

KIEM TRA SAU KFII XE THIT

PI-IAN EOAN -NHAN DINH

atO)

l. Rii d6trg (tip d4tng v6i fim ding lgnh)

Cho rnAu vAo tiri PE kin rruoc, ditng ddy buQc chdt n-riQng tiri, b6 vdo thirng, chQu

thich hop rdi cho ddng nu6c sach chAy tiL du6i lOn v6i h,rr"r lugng kh6ng ldn hon 20

lit/phirt. Khi bdng vira tan h6t (nhiQt dQ m6u gdn tuong duong v6i nhiQt d0 m6itrudng), thuc hiQn c6c bu6c ki6n.r tra t4p chAt d6i v6i m6u, bao gdrn ci phdn ntr6c do

tan bdng.

2. lti1m trt tirth trqng bAn ngorii cfia !6m:

- Quan s6t t6ng quat todn thAn cho c16n chi ti6t tirng bQ qhQn bOn ngoiLi cira t6rn

theo tlinh ttr til dAu ddn ciu6i bao g6rn cdc bd phAn: virng cldu irc, ndp rnang, 16 he,

thdn, v6 bUng cl6t r hodc d6t 3, ch6n bung, c6nh du6i, gai du6i.

- Chri i quan s6t vd ghi nhAn nhirng bi6Lr hi€n cdm quan b6t thudng tr6n m6u vdt

kh6o sdt, ddc biOt 6 cdc vi tri virng d6u ric, nip mang, d6t thAn tl.Ii 3, cdnh dudi vir g-ai

dudi, cl6i cLi6u cac quan s6t vd ghi nhfln v6i nhrrng mO ti bi6u hi0n cAm quan di6n

hinh cira t6rn c6 born tap ch6t.

3. tfiEm trn suu ltlti bdc vti ttiu tit':

- cam r6m d6c clau xu6ng c1u6i, dirng tay b6c v6 ddu irc t6rn dti 16 ra ph6n thit dau.

Hirng phdn dich dgng trong xoang uo ddu u" cho v?ro dia petri dC c6 thd ti€n hdnh

ki6m tra ph6t hiQn nhanh tap chAt bdng phuong phdp h6a hoc khi cfur.

- Di.rng rnfri dao nhon kh6o 1eo lat va g?t kh6i gqrch (gxn tuy) leD dC lim 16 xoang

oau 0c.

- Ding mfri ngui dC ph6t hiQn xoang diu uc c6 mui l4 hay kh6ng'

- Quan s6tlinh trang xoang cliu irc c6 dqng ch6t.dich khA nghi hay kh6ng. Dirng

thia nh6 mfic l6y phAn dich dong trong xoang dAu irc (ni!u c6) vd cho vdo dia petri sach

dC c6 the ti6n hdnh ki6m tr.a ph6t hiCn nhanh tap chAt bang phtLong ph6p h6a hoc khi

ca.n.

- i(i6rn tra trang th6i l6p dich l6y_ dLlo-c t* xoa'g dAu ric cira m6u v{t (mdu, rniii,

hinh dang, ket cau), c16i chiCu vdi bi6u hiQn d.4c trung cta t4p chdt hudng dan tuong

iLng dutii r16y d6 x6c clinh bu6c ddu lo4i tap chAt dd ductc dr"ra v2ro t6rn.

- Di.g ng6n tay ki6m tra ch6t dich b6'.r tr6n phAn thlt diu t6rn vd xoang dAu irc dC

ph6t hiOn"nhing bi6u hiOnbAt thuong (rt'tiLc d0 dinh, nhot), n6u c6.

4. Kiiim tru cd thlt t1nt [Id bdc v6:

- Ding tay ki6m tra chdt clich bdrr trdn phAn co th!1 than L6rn d6 phat hiQn nh0ng

bi0u hiQrr bit LhLrong (nruc do c1inh. nhot), nOLr co

- Quan s6t vd bd r.rgodi ctia thAn tdm, ddc biQt o c6c dot thit thu 3, 4 v?r 6 xem c6

bi6u hiQn cira su phr)r ni: c6c ddt co hay khdng.

- O nhtng m6u t6ni bi bom t4p chat v6'i liOu ltLo-ng lon, c6 th6 th6y lO c6c d6t co bi;. ^.phir il6. Dirng kirn chanr vAo vi t|i bung hay lrrng dot co co bi6u hiori bi phu nd vd ldy

tay ndn c6 the thay tap chat ditn ra.

5. Ifidm fta sau khi xd thit:

- Dirng dao b6n xd doc thAn t6m til c16t dau dOn d6t duOi.

".-: ,,,,.- i "- Dung rnii ngui ile phat lllell vet xe co Inul ta nay l(nong.

- euan s6r c16u v6t ctrAt cticn dinh tr6' ludi clao. OOi cnieu v6i cac 'ro td bi6u hiQn

da, ;;;';;;ii"n ii^r1 tr6n lr"rdi dao d t6n c6 t4p ch6t d0.xdc dinh c6 tap chAt trong

mAu v{t "nay

khdng vd ph6n do6n khi ndng d6 ld loiri t4p ch6t gi'

, Dung s6ng clao cao nhg b6 mdt vi5t xe va c6c khe thit, quar.r s6t s6ng dao c6 dong

chAt dich khd nghi hay kh6ng.

- Dr'rng ngorr tay carrr qrrrn chAt clic.h b6ru trdn s5.ng dao.dd.cAm nlrdl=nli1ng frieu

hiqn cldc ri*U t'nii. cio dirifr. nlrot ). Doi clridtr voi c6c nr6 ti bi6tr hiqrr ddc t'trng cua

t4p ch6t d6 ph6n doirn khh ndng cl6 ld loai tqrp chdt gi'

6. Phtirt ilotin - NhQn cllttit:

Tr0n co sd nhirng k6t qud ki6m tra vd chring cir thu th{p o cac b.uoc ^trOn'

nhdn

vi6n ki6rn tra c6 th6 su dung So d0 c5c bu6c x6c dinh t4p chat lrhu la rnot cong cu

h6 tro- cho vidc phfn dodn, nhfln clinh t6m c6 tap chit hay kh6ng'

o. l. Su-. dd cdc buo'c xfc (finh tap chat:

PHAN EOAN TdP CTIzIT:

- KhOi dich keo, sQt, c6 h4t li ti?- Kh6i dich nhd'n tron b6ng, c6 h4t li ti?- KhOi dich nhd'n, h4t li ti, 6ng inh?- Kh6i dich c6 miru sim, nhd'n, c6 miri 14?

- Kh6i dich tring clqc, keo dinh?

-> NIIAN DINII BUOC DAU theo Mttcl '2,1 '3 vitl 4

6.2. BiOu hi€n chung t6m c6 taP ch6t:

a. Edu: ph6ng, ddn, nh6;

b. Ndp mang: Phiing, ng{r.r'r nudc;

c. Phdn v6 bung dOt t no4c c16t: co th6 truo'g phdng' ng{m 'udc (n6i vAy);

d. Dand6t3;

e. 'l'hAn: hoi cing d6n cdng trdn;

f. Carrh iltroi xoe, gai clLr6i v€nh;

g. C6cbQ phin kh6c (c6nh du6i, 16hq, ch6n boi): c6 bi€u hiOn ngdm uuoc'

6.3. BiOu hiQn d4c trung cira t6m c6 tap chAt:

VI'TRI ACARAGAR +

CMCAGAR+

PVAAGAR +ADAO

TINHBOT

C6 dichlodng, hoinh6t

C6 dicltlodng, hoinhdt

C6lodng,nhot

dichhoiGiira v6 vi

mang oau

ric

C6 dichlodng, hoinh6t

C6 dichlodng, hoinh6t

Xoang ditt(du6i

ganucg4ch,

tuy)

C6 dicltnhdn, hoi s€t

dt5n sQt

C6 dichnhdn, lodngcl6n sQt

C6 dichnhdn, lodngdOn sQt

dicl.r

den

C6nhdn,loflngs9t

C6 dlch. : ;, ,.

nnay, ral lr,lodng

Phirdichnhonnhdn

n6, c6hsioen

Phu ne, co

dlcn nOl

nhdr.r d6n

nhon

Phil nO

kh6ng 16,

c6 dich hoidinh

Co thAn cohoioen

Phir n0,

dichnhdnnhdn

n6, cohoioen

Phndichnhdnnhdn

C6 th€ thAY

kh6i t4p chAt

dirn ra

uo tne tllaykh6i qpchat dun ra

I(hOngthdytapdin ra.

l&6i^uA+

D6t thir 3

(chich van?nl

thAycnar

thtap

C6Knordtn ra

C6 th€ thhY

kh6i t4p ch6t

dun ra

- Hoi u6t |

- FIo'i u6t

- C6 dinh | - C6 dinh

clich nhdY, I dlch nhAY,

n6i hat li ti I ndi hat li ti

- c6 lftoi | - c6 kh6i

dich nhdn. I dich nhdn,

sd tay hoi lsd taY hoitroq nhdn I tron nhdn

- Hoi kh6dinh

- C6 it chdtdich, sd c6cim giircdinh

-Khoi dichc6 h?t, sdtay hoidinh

Co'thlt bentrong:

^l- Be lnatdao x6

nh9song

- C?oDang

dao

- Hoi rdo

- C6 dinh,ldrcn nnay.:.. ,. .

nol hat ll tl.' ' i.- Lo KnOl

dich nhAy, sd

tay hoi dinh

- Hoi ud(.

- C6 dinhdich nhAY,

n6i hat ti ti.,,

^'- uo Knol

dlch nhdy, sd

tay hoi nhon

6.4. Bi6u hiQn dic trung cita t?p ch6t c6 trong tdrl:

TI-IONGSO DANH

GIAAGAR

AGAR +CMC

AGAR + PVAAGAR+ADAO

TINHBOT

LVIA UTring ' d6ntrang ouc

Trdngtrong cl6n

Lfang ouc

Trdng trong d€nhoi duc, c6 hat

nho hoi 6ns dnh

I lang oucc15n hoiclen

ouc

MniKh6ng phathi6n

Iih6ngph6t hiQn

Kh6r'rg ph6t

hi6n

lltLl I

ddcC6h6itrung

KhOngphrit hiQn

Hinh d4ngiTr4ng th6i

- Keo, sdt alen

dac set- bich co nat tlri

- sct- ( nat orcntro-n b6ng

I-.odng c16n s6tLodngoen set

Lodng

I(6t cALr NhdnNhon, dinhray

NhonNhon, hoinhAy

Hoi dinhoen olnn

vI. KIiIM TRA NFIANII BANG PHI'ONG PHAP HOA HE}C

1 Nguy0n tdc

- phuong ph6p ki6m tra '5anh

tap chAt n6Lr trong tdi liQu ndy dua tron stL qr.tan s6t

cdc bi6n aOitia"g th6i clac trr-,rg xay .a trong phd' irng gifra t4p chdt 'd

dLmg dich

Llruoc Lnu.

C6c bi6n d6i tr4ng thii ilic trung cfia cric loli t4p chit

Lo.1i t4pchflt

Dung dichthudc thir t4p

chflt

Thirnh phAndung dichthu6c thii

Sq bi6n ddi {l[ctrung

Co'ch6 s1t biiin d6i

Tinhb0r

DrLng dicht1-ru6c thir tinh

b6r

Iod t.,()1 nlaU tu llaudo sang xanl.r

den

Iocl Ien vdo crc tnach dang xodn c[ta

tlnh bol tao tllann plluc Den. maLl

xanh den

PVA Dung dichhu6c thiL PVA

Iod tlong acidH:llO;

tJor mall tu vang

d6 sang xanhTrong str hiOn ciitn cira acid H:BO: ,

PVA t6c dung voi iod tao thdnh phticbdn. rnZrrr ranlr

-

Agat' lung dichthu6c thfr r\gat

lod iloi lllaLl ILr naLt

clo sang tim cten

Du6i s1L gia nhi6t cho phan irng (tr6n

50'C) agar t6c drurg vcri lod tao thdnhphirc bdn ddc trung mdr,r tim den

Adao Dung dichthu6c thir

Adao

Acid tannic D6i mdu tit vdngnhat sang k6t tira

tring

C"tatin O1 k6t tira trong dung.dichacid tannic

CMC Dung dichthu6c thiL

CMC

Axitcl'u'omotropic

Dr"rng dich thu6cthiL c6 m?ru tim

tai ldp phirn clLch

Du6i sp gia nhi€t (70+5'C) cho phirn

iurg CMC t6c dLrng voi Axitchlornott'opic tao Lhdnh phiLc bdn d4c

Ltung tn?rll tini.

Agar(Trong

tltlttdm)

Dung dichr-toc rrL

Beneclict

loHsOrNa:NazCO:llLrS04.5H:O

lJor lnau tnuocthir tt xanh saug

xanh 16, k6t ttra

rlo gzich dlctf ur'tg

frotul..g ai"n thu6c thir Benedict,

Agar bi kat k6t -tita rndu vdng il6 gach

vd lrim thay ddi nriu mdi trucrng tuxanh saug xanh 16 tiry-udng dd agar

c6 tt ong tnitt'

-4_

2. Phuo'ng PhfP thfl nghiQm

2.1 Chadn bi mdu firt':

- l,ua chon c6c m6u t6m c6 bi6u hiQn da bl born chich t4p ch6t th6ng qua phuong

onao t-'#,r.r iru Uine carn qtrarr clLLoc ueu o Phin V. p6i v6i 'riu t6rn dong lanh, Lhuc

iie,ifiA* tra tap cf,ir ct6i voi mAtL. bao gdrn cd phAn nLroc do tan bd'g.

_ Tiy thuQc rlao loai, h2Lni luo-ng vd vi tri tqp ch6t trong_rr6u, ti5n hdnh tlitL nghiQm

truc tiep tai c6c vi rri thrrong tdp trung tap chat tron t6m.nhu xoang clAu,ahan tdm x6

dqc tdoi voi rap chAr 16 tintibqi. PVA) ho?c. chuy6n dich tqrp chit vao^din pelri hofc

a"g "gt

icln tr.uo,c khi thu nghi€m (clac biqr cl6i voi t4p chat ld agrr, cMC vir gelatin)

2.2 Thu'nghiQm tqi xouttg rtiu tim

D6 thu nghiQrn phdr hiQn tqp chAt tqi xoang clAu, ti6n h?rnh cac buoc chuAn bi tdm

nhtt sau:

- Dirng tay tr6i nim lAy th6n t6m, huong cldu tdm xuong dtr6i'

- Nhe nhirtg boc tdch phirr vo o cliLr L6nr'

- Dirng dao cdt nhe 2 berr ddu t6rn'

- Gar b6 phdn g4ch t6m ele 16 phdn xoang dAu 'oi tip trung nhiALr dung dich tap

ch6t. Cnl i kirdng l2rnr be grch tdtn vA rrrdt t4p chAt tLong diu tdnr'

- Tidn hdnh thir nghiQn.r theo Mr--rc 2 5'

23 Thrt'nghigm trli vi tri tlrAn firn

E0 thfr nghiQm phdt hiQn t4p chAt tai ihdn t6m, titin hdnh c6i budc chuAn bi t6m

. nhu sau:

- Dung tay trdi narn chat l6y LhAn t6rn'

- Nhe nhdng b6c t6ch todn bQ phAn v6 t6m (et6i v6i tdrn cdn v6)

- Ding clao cir b6 dAtr L6m (d6i v6i t6rn cdn ddu)

- Dirng dao xd doc thdn t6m tir dOt OAu cten AOt CuOi'

- Md rong than t6rn, clir*g kinh lirp x6c dintr vi tri tap trung nhidr-r tap c66t d6 thu

nghi6m.

- Tien hdnh thu nghiQm theo Muc 2'5'

2.4 Tttrt'ttghi(m trLn cfiu petri/ing nghiQm:

_ Ti6n hdnh c6c budc chuan bi mlu t6rn c16 thir nghiQm nhu neu trong Muc 2.1.

- Dirng claoimu6ng chuy6n dich t4p ohAt trong xoang dAu t6m vdo dia petri/6ng

nghiQm d6 thiL nghiQm.

- Ti€n hdnh thil nghi€m tlieo Muc 2'5'

2.5 Ti6n hitnh thti'nghiQm:

I-dn ltrot sir d'ng c6c dung clich thudc thir t4p ch6t dd chuAn bi 6 Phdn III muc 3'5

dC'ph6t hiQn t4p ch6t c6 trong t6m, theo cdLch nhu sau:

2.5.1 Tlit nghiQm phdt hipn tinh bQt:

a) Nh6 01 giot thu6c rhrL tinh bot vdo c6c vi tri tap tnng t?p chAt d6,x6c dinhvd' ,,aw

chuAn bi ir muc 2.1 (xoang etAu' thAn tdm x6dgc ho4c dich t4p.chAt.trdn dia

ili. ii; fOi "4, "i tro,,g 16' s0 phan ung rar

'nanh v6i du'g dich thudc thri

i4o phric bdn n-riLr-r xanh den'

chuy6n rndu cira rhudc thir tlong thu nghi6rn tr6n mAu

c)

2.5.2

a)

tnu

Ti6n hdnh ktlr lua' vd srL hi6n di€n cira,tinh bqt theo Muc 3 (doc k6t qua)'

Thrt'nghiQm Phdt hitn PVA:

Nh6 01 giot thu5c thiL PVA vdLo cdc vi tri tQp trung tao ctr6t cld xac dinh vir

chuAn bi 6 bu6c 2.1 t"ou,tg aAt', inan ionl tl'aqc hoA;giqt t4p 9$1 trcn'm'a

o.tri) fup chat Pva ncu J6 tr.ong t6n sd phdn irng r6t nhanh v6i dung dich

ihu6c thri tao phirc b€n m2ru xar-rh'

Quan s6t vir so s6nh su chr-ry6n n-riLu cua thu6c thtr tlong thu nghi€nr trdn mAu

thiL.

Ti6n lidnh krit lr-ri. vd sLr hiQn diQ' cira PVA theo M'c 3 (doc ]<ot qu[)'

Th{r'nghiQm Phit hiQn Agar:

Ding dao/mu6ng cl.ruy6n dich tap chAt.tr)r.trong xogng cldu t6rn' thAn t6m viLo'2

6ng nghiQrn tlitiy tinh itr0m t<t.toang hnl ntLdc cAt vAo tt't6i 6ng nghiOm Ldc

trOn ddtr bdng taY r Phrrt

D6nh dAu thfr ttr cl€ phAn bi€t hai 6ng nghiQm'

Ong thir nh6t dun nh9 rrdn nggn ddn cdn hodc trong bd di€u nhiQt < 40'c hoflc

d6 d nhict dd phdng.

b)

c)

2.5.3

a)

Or.rg nghiOm thiL hai dLrn s6i trOn ngon ddn cirn khoAng z phirt' ho{c lttY?

hi:"

u"i"U? c1i0u nhi6t. Dun d6n khi di; mdu tan ch6y hodn todn vZt it nhat bit dau

ifrav"ruat f-rie" cli6m sdi thi n6i c6 th6 ngirng qu6 trinh dun lai'

D0 nguQi cdc i5ng ngiriOm cl6n nhiQt dQ phdng' Ti6p t\rc nho 2 el6n 3 gigt thu6c

thir vdo c6c 6ng nghiQm, aga' n6u tO t*ng toil o 6ngnghiQm thir 2 sO phin fng

tAi nftunft vdi dirng dich thtLoc thu tao phirc bdn m?Lu tim den'

Quan s6t vir so s6r-rh su chuydn rniru cria thu6c thri trong thiL nghidm tren rnAr'r

thtI.

Tign hdnh ki5t l'4n v6 sU hiQn cliQn cira agar theo MUc 3 (dqo i(Ct qLla)'

Thi nghiQnt Phit hiQtt gelatin:

Nh6 01 giqt thu6c thir gelatin viLo c6c vi tri tflp trung tap chdt da x|c.dinh vi.fr.A" iiO'U"oc 2 1. Ta"p ,1lai getatin n6u c6 trong tOn phin ring rAt nhanh v6i

clung dich thu6c thiL t?o ket t[ra trdng'

QLran s6t vd so s6nh qtr6 trir.rh k6t tira cira gelatin trong thu6c rhir khi thir nghiQm

trcn rniu thu.

c) Ti6n hdnh k6t luin vd sr,r hiQn diQn c6a Adao theo Muc 3 (dqc k6t quA)'

2.5.5 Thft nghiQm Phtit hiQn CMC:

a) Di.rng dao/mu6ng chuy6n dich t?p ch'4t tir^tlong xoang dAu t6nl.tlan t"]1,::-'

; 6;e"ghic* ttr"Lry tinrr. Th€m khodng l-2ml nu6c cAt viLo n6i 6ng nghlem.

7Lictr6nd6ubingta)'30gial fu

b)

c)

0)

f)

c)

2.5.4

a)

b)

b) E6nli cl6r.r th(L tg t10 phdn biQt hai ilng nghiomThdnr 0'5 ml clung clicli thu6c

thir axit chrornotrop'ic vdo 6ng nghiQrn iao cho tao thdnh hai 16p ri6ng biQt.

OJ J" A"g "gf.iQni

vdo bC n''rdc n6ng khodng 70+50C' thdi gian 15 phitt'

c) Quan s6t vd so s6nh su chuy6n mdu cria thu6c thu trong thir nghiQm trOr.r mdu

thiI.

2.6 Thrt'nghiittt ttli phbng ki6m nghiAm:

2.6.1 Thti'nghipnt phdt hi\n Agar trong thlt tOnt

Su'dunp.c6cdungdichthu6cthriph6t-hidllagartrongthittornddchuAnbitaif.n"a" .l,fia"l i Z pna" Uf de phar hien tap ch5L Agar co trong tom' theo c6ch nhrr sau:

a) CArr 39 rn5u thit tdm cld duo-c d6ng h6a vdo 6ng nghiQrn thiry tinh'Thdm lml'

clung dlch acicl hydrochloric d6ru c14i' Dun s6i trdn ngon ddn cOn khoang 30 dOn

60 giAY.

b)I-?rmnguQi6ngnghi6mo-nhiQtphdng'FlirtlmldrtngclichrrrSr-rv2ro6ngnghiQmrhirY tinh khtic.

c) Trung hda rn[u bdng cich cho viLo 2r.nl clung dich NaOH 10% vdo 6ng nghiQm

k6tn tra PFl= 7 .

ffh0n'r 5ml dur-rg clicl-r thr-16o tl'riL Benedict, dun s6i.tr0n nqol clel c6rr lihodrng 30

,t€n OO giay (hJ4c clun s6i trong Ue AiOLr nhiQt), d6.nguQi' T4p chdt Agar n€u c6

i."gif.1, lu'phln fng v6i thu6c thiL ldrn thay d6i mdu thr6c thir tiL xanh sang

xanh 16 vd k6t tira ndu d6 ddc trung'

Quan s6t vlL so s6nh qu6 trinh k6t tira cira Agar trong thu6c,thir khi thri nghiom

tr€rt nr6u thil va tren tn6rtt trdrrg'

Ti6n hdnh k€t ludn vd srL hidn di6n cria Agar theo Mpc 3 (doc kdt qua)'

Thil'nghiQm phtit hiQn CMC trong th/ fint

Dung dao/rnudng chrry6n dich t1p chat tu trong xorng ddtr torn viro dyng:U

"i,tu] ro,rr b6c * cho vdo in6v ity 91ig^*0'1g,vdo

i5ng nhira 10Tf '1c,*5ml nu6c cAt. Lac cliiu, ly t6m vOi t6c AO 3000 vdng/phirt, thdi gian.5. pht I-i1

;il";*; fuu rren clio'vdo 6ng nghiOm thiry tinh Lic trQn c16u bdng tay 30

il;" t- tir 0,5 ml tlLrng clich thLr6c thir axit chrouotropic vt\o 6ng lehigy 3ao

cho tao thdnh hai l6p riOng tiet pC c6c 6ng nghiQm vdo b6 nudc n6ng khoAng

e)

1)

2.6.2

a)

b)

70+50C, thbi gian 15 phit'

c) Quan s6t vir so s6nh su chuy6n m2Lr-l

thu theo bAng 3.

d) Dqc kOt qua

ctra thu6c thri trong thir nghiQrn tr0n mdu

Sir dung b6ng sau dd KeL lufln su hiQn diQn criatap chAt co tfong tonr

I{iQn tug'ng xiY ra trOn mAu thii'

--._--E6i r-ndu tir d6 sang xanh den

Ki5t tu4nLo4i t4p ch6t Thu6c thii'si'r'

. dgng

'finh b0t Thu6c thir Iod

C6 tinh bOt

_ _Kn:'t: cldi rndLr

_DOi mdr,r tiL viLng ct6 sang xanh

Kh6ng c6 tinh bdt

C6 PVA

PVA Thu6c thil PVAKh6ng d6i mdu

E6i mdu tir nAtl do sang tirn den

I(h6ng c6 PVA

C6 agar

Agar Thu6c thfi AgarI{hdng d6i mdu

I(Ct tira tlingtt u-:<^^ ^A l. Lt t,',at\l LUIlB \,v Nvt !Lr(r

:.Dung dich lhtrdc lltil xr-rit hi0n nir\tr

tim tai l6P Phdn c6eh

I(h6ng c6 Agar

C6 Gelatin

GelatinThr-r6c thir

Gelatin I(hOng c6 Gelatin

C6 CMC

CMCThur6c thtL

CMC : .,..^ a

Dung dich ihuoc thft lthong xttiithi€n mdu tim tgi l6P PhAn c6ch

Kh0ng c6 CMC

Agar (trongthit tOrr)

Thu6c thirBenedict

D6i mdu t xanh sang xanh ld' I(6ttira niu do

.:.Kh6ng d6i mdu vir lihOng kOt tua diLc

tn rno

C6 agar

I(r0ng c6 Agar

CMC trongthit t0m

'fhu6c th[L

CMC

Dung dich thu6c thri xu6t hiQn rndu

tirl fai lir-r'r ohAn cdchC6 CMC

Dung clich thu6c thu kh6ng xuAt

hi€n mdu'tirl tai l6P PhAn c6chKhdiig c6 CMC

e) Ddun bao chAt luong:

Khi thir nghiQrn mdu tring, dung dich thu(5c thfr.kh6ne."ltuy.tn:3tt99i v6i c6c

ph6p thf' .'in[ UOt, PVA, CilC, AfaL vd lihdng xuAt hiQn k6t tira d6i v6i phep thti

Adao.

vil. GllI BIEN BAN KV riltj+'rKtit qu6 ki6rr tra c6m quan vd h6a hoc phdi.duo.'c ghi day dir vdo Bier.r b6ri k! thu{t, k6t

1u0n i6m c6 t4p chAt hay khdng, lo4i t4p chAt gi

(ban hdnh kdm theo

cfia 86

TEN CO QUAN KIEMDOAN KIEM TRA Tf

QUYET DINIf SO:...{'r

^1

'-RNNPTNT' ngdy //Ahang+-ndm 201 B

vd Phat tri€n nong th6n)

A xA HgI CHU NGHIA VIET NAMDQc l{p -'f r.p do - H4nh phirc

BIEN BAN KV TI{UATKrEM TRA CiM QUAN VA Tsrl'nhaNH HOA HOC

'fAP CHAr rRONG ToMS6:

I. THONG TIN CHUNCT6n chu hdngA{gudi vdn chuy6n hdng:

Dia chi:

Ching lo4i nguy€n liOu: Phuong ti€n vAn chuy6n:

Sd luong: I(h6i luo-ng l0 tdrn:

'' . '.;l\gay Klem tra: Dia cli6m ki6rn tra

PhAn dAu irc f Ph6ng, ddn, nh6 f einh ttrucng

Ndp mang ! Ph6ng, ngAm nu6c ! Binhthuong

Vo phAn bung I t t\ol vav. rlqallr mroc f] Binh thu<mg

Phdn thdn ! Cang tldn, din d6t f-l Binh thrrcmg

PhLr b6 dudi f] C6nI clu6i xod, gai v6nh I ainhthuong

C6c phu b6 kh6c 16 he, chdn boi I l Phdns. nsdrn mr6c -t-.,.,.Ll rJlrrn lnuong

Giira v6 vd mdng dAu irc f Cd cticlr nlron f] Binh thuong

Xoang dAu irc I C6 dich nhdn i nhAy / sdt I einn tUuong

B€ mdt co thAn f co aicn nnon fl Binh thuong

Chich vd ndn co thdn ! cO Cicfr nnon / nnAy / sQt f] Binh thunng

Bd mdt dao x6 I C6 b6n1 dich nhdy / n6i hat I Binh thuong

86 mat co thit ch5 v6t xe ! CO alcn nhAy / nhon / dinh I Btnh thuong

Mau, nrrhi cia chdt dich f C6 mdu Ia / miri la f Binh thubrg

Trang thdi chAt dich ! Keo/s€t ! Tron/b6ng ! CO tr4t I Dinh

rTt.oltgtr.uonghopkiinltr.avi€ndtto.cgiaoki6rnrr.a|ohdng;chicoquanki6nltraphdnc0ng.giaonhiQmlu

ki6m rlr vi6n '/

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