Chromatography

Module Selection Guide

NGC Chromatography Systems

Fig. 1. NGC Discover™ System.

The NGC family of medium-pressure chromatography systems offers a single solution that scales to fit your purification requirements.

The NGC system’s truly modular design provides multiple configurations to align with the requirements of every individual scientist.

The NGC instrument’s small footprint means it can be used on a lab bench, in a laboratory refrigerator, or in a cold room.

The 10 ml/min and 100 ml/min preconfigured instruments — ranging from the NGC Quest™ to the NGC™ Discover systems — are designed with increasing automation and throughput capabilities to serve a wide range of laboratory needs.

The magnetic column clamps allow you to mount the columns exactly where you want them. This minimizes the bench space needed for traditional column holders and simultaneously reduces long tubing connections.

The pumps, detectors, and pH monitor all have LED displays to show real-time status and module performance.

DESIGNED BY YOU. BUILT BY BIO-RAD.

TRULY CUSTOMIZABLE

The forward-facing modules allow easy-access plumbing of the system. Each system is completely open to redesign, allowing individual customization to meet your needs and application requirements.

EXPANDABLE

Expand your system capabilities with the addition of tiers and modules. Modules can be moved and tiers can be rotated, letting you position all components for maximum convenience and efficiency.

© 2020 Bio-Rad Laboratories, Inc. | 3

Buffer tray

Mixer

Gradient pumps — 10 ml/min or 100 ml/min

Inlet valve —buffers

Sample inject valve — compatible with loops and sample pumps without the need to replumb

Inlet valve — samples

Touch screen — manual control for monitoring method runs

Integrated sample pump — automated sample loading

Magnetic column holders — attach columns anywhere you want

Column switching valve — includes bypass and reverse flow capability, for connecting up to 5 columns

Outlet valve — flexible fraction collection options

pH monitor — with integrated bypass channel and calibration port

Status displays — provide real-time system information

Detector with integrated conductivity monitor — single- or multi-wavelength, for simultaneously measuring up to 4 wavelengths

Buffer blending valve — automated inline buffer preparation and pH gradient

THE NGC LIQUID CHROMATOGRAPHY FAMILY — DESIGNED TO MEET YOUR SPECIFIC PURIFICATION NEEDS

The NGC family of medium-pressure chromatography systems offers a single solution that scales to fit your purification requirements.

The NGC System’s truly customizable design provides multiple configurations to align with the requirements of every individual scientist.

The NGC instrument’s small footprint means it can be used on a lab bench, in a laboratory refrigerator, or in a coldroom.

Each system comes with a touch screen, providing instant status updates and manual control of system functions.

The 10 ml/min and 100 ml/min preconfigured instruments — available as the NGC Quest, NGC Quest Plus, NGC Scout, NGC Scout Plus, and NGC Discover Systems — are designed with increasing automation and throughput capabilities to serve a wide range of laboratory needs.

The magnetic column clamps allow you to mount the columns exactly where you want them. This minimizes the benchspace needed for traditional column holders and simultaneously reduces long tubing connections.

The pumps, detectors, column switching valve, and pH monitor all have LED displays to show real-time status and module performance.

NGC Discover System

4 | Module Selection Guide

System Pumps A chromatography system with multiple users, applications, and purification requirements often uses a variety of column types and sizes. The NGC Chromatography System enables you to swap out pump modules to meet your specific requirements.

The NGC family of systems can house up to three high-precision pumps: two system (gradient) pumps and one sample pump, all integrated within the instrument.

The system pump modules include a display for real-time monitoring of pump status, flow rate, and pressure readings.

PUMP INLETS AND AUTOMATED BUFFER BLENDING

NGC SYSTEM CAPABILITIES SCHEMATIC

F10 Reciprocating Piston Pump■ Flow rate: 0.001–10 ml/min at

3,650 psi (25.2 MPa)

■ Ideal for small-scale preparative purifications

■ Can also be used for HPLC separations

F100 Reciprocating Piston Pump■ Flow rate: 0.01–100 ml/min at

1,450 psi (10 MPa)

■ Flexible flow rate range

■ Ideal for scale-up applications

Catalog # Description7884002 NGC F10 Pump Module7884003 NGC F100 Pump Module

System pumps

Inlets, blending valve, and system pumps

Sample loading Column switching and reverse flow

Sample monitoring Fraction collection

x 5

Water

Salt

Acid

Base

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x 8x 8

Water

Salt

Acid

Base

l 280

nm, m

AU

800

700

600

500

400

300

200

100

0

0 2 4 6 8 10 12 14 16 18 20Volume, ml

pH 8.5

pH 7.5

pH 6.5

Buffer Mixer ModuleThe NGC Mixer Module homogenizes buffers from the two system pumps (A and B) and includes a mixer motor assembly as well as an integrated system pressure sensor.

Gradient Flow Rate Range■ F10 pump: 0.001–20 ml/min

■ F100 pump: 0.01–200 ml/min

Gradient Composition Accuracy■ F10 pump: ±0.5% (3–97%B, 0.25–10 ml/min up to 3,650 psi/25 MPa)

■ F100 pump: ±0.8% (5–95%B, 1–100 ml/min up to 1,450 psi/10 MPa)

Mixer Volume ■ 263 and 750 µl barrels included with the F10 pump

■ 750 µl and 2 ml barrels included with the F100 pump (5 and 12 ml barrels available)

Catalog # Description7884018 NGC Mixer Module

Buffer Inlet ValvesOptimizing separation conditions can be a time-consuming process, especially if you have to switch out bottles and tubing each time.

The NGC Buffer Inlet Valve Module enables automated switching between buffers to accelerate method development, column cleaning, and regeneration.

Buffer Blending ValveAchieving the correct pH required to perform most separations can be challenging. The NGC Buffer Blending Valve speeds up buffer preparation and enables pH scouting by automating inline buffer preparation.

Using acid, base, water, and salt stock solutions, the system can generate specific pH buffers over a linear salt gradient or a pH gradient at a specific salt concentration.

pH scouting with a cation exchange standard containing a-lactalbumin, ribonuclease A, cytochrome C, and lysozyme in a simple buffer mixture automatically titrated to different pHs.

■ No need to replumb between runs

■ 8 buffer inlets per valve

■ Inlets can be labeled within the software

■ Inlets can be dedicated to cleaning and storage solutions

■ Each system pump can have an 8-port inlet valve

Catalog # Description7884006 NGC Inlet Valve Module

■ Enables inline buffer preparation

■ Enables pH scouting

■ Multiple preprogrammed buffer recipes

■ Buffer pH range: 2.7–10.3

■ Maintains full gradient capability

■ High flow capability

– F10 pump: 20 ml/min– F100 pump: 200 ml/min

Catalog # Description7884010 NGC Buffer Blending Valve Module

System pumps with inlet valves

System pumps with buffer blending valve

Whether you are loading microliters using a static loop or liters using a dedicated sample pump, the NGC System offers a complete range of options to minimize the risk of sample loss, to automate sample loading, and to prevent the introduction of air into your system.

SAMPLE LOADING OPTIONS

Sample Injection Valve■ Accurate sample loading

■ Compatible with fixed volume loops, variable volume DynaLoop Sample Loops, sample pump, and autosampler

– No need to replumb – Sample volumes: µl to L

Catalog # Description7884007 NGC Sample Inject Valve Module

Sample Pump ModuleAutomated sample application can be achieved using the NGC Sample Pump Module.

■ Dedicated pump eliminates contamination of system pumps

■ Ideal for automated large-volume sample loading directly to the column

■ Repetitive accurate sample loading via a fixed volume loop

■ Connects directly to sample inject valve without replumbing

■ Flow rate: 0.01–100 ml/min at 1,450 psi

■ Flow rate accuracy: ±2%

■ Sample viscosity range: 0.5–10 cP

■ Integrated pressure sensor

■ Multiple large volume samples can be loaded sequentially using up to 2 inlet valves

Catalog # Description7884004 NGC Sample Pump Module

Sample Inlet ValveAutomated sequential sample loading of multiple large-volume samples can be achieved using inlet valves with the sample pump.

■ No need to replumb the sample pump between samples

■ 8 sample inlets per valve

■ Inlets can be labeled within the software

■ Up to 2 sample inlet valves can be used with the sample pump

■ Module accepts up to 4 air sensors

■ Compatible with large- and small-bore sensors

■ Air sensors map to fluidic scheme and provide real-time sensor status

■ Air sensor extension module available for additional sensors

Catalog # Description7884006 NGC Inlet Valve Module

Air SensorIntroducing air into your system can damage columns and waste precious samples.

The NGC Air Sensor Module enables the detection of end of buffer and sample, thus protecting your system and saving you time.

Catalog # Description7885017 NGC Air Sensor Module

Note: Third-party autosamplers and external detectors can be used with the NGC System via the NGC Signal Import Module (catalog #7884016).

Sample inject valve with sample pump

Sample pump with two sample inlet valves

Fluidic scheme with air sensors

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CEX — 8 µm

l 280

nm, m

AU

300

200

100

0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Volume, ml

CEX — 10 µm

l 280

nm, m

AU 400

200

0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Volume, ml

CEX — 50 µm

l 280

nm, m

AU 150

100

50

0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Volume, ml

l 280

nm, m

AU

CEX — 70 µm

Automated column scouting using the column switching valve with a cation exchange standard containing a-lactalbumin, ribonuclease A, cytochrome C, and lysozyme in phosphate buffer, pH 6.5, separated over four cation exchange (CEX) resin columns of various particle sizes.

120

80

40

0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19Volume, ml

Column Switching ValveManual column scouting to determine optimal purification conditions is complicated and time consuming. The NGC Column Switching Valve enables automated column scouting.

Up to five columns can be connected without the need to replumb the system. The valve also includes an internal bypass function for priming or cleaning your system.

When undertaking affinity chromatography, it is desirable to shorten run times and elute concentrated products. The NGC Column Switching Valve has a reverse flow function ideal for rapid elution and sample concentration from affinity columns. This feature also enhances column cleaning and can increase a column’s lifetime.

Avoiding column resin damage from compression is an important concern of researchers. The NGC Column Switching Valve has integrated pressure sensors that measure pre-column and delta-column pressures. This protects the column and resin from compression by triggering the pumps to pause or reduce the flow rate.

B

A

COLUMN SWITCHING AND REVERSE FLOW

■ Automated column/resin scouting

■ Connect up to 5 columns

■ Real-time display shows pre- and delta-column pressure

■ Reverse flow functionality

■ Internal bypass flow path

■ Integrated pressure sensors

■ Column storage

■ Install up to 3 column switching valves

■ Enables tandem and Multi-D applications

■ Use as a loop valve for Multi-D applications

Catalog # Description

7884012 NGC Column Switching Valve Module, 10 ml7884026 NGC Column Switching Valve Module, 100 ml

A, column switching valve; B, column switching valve reverse flow.

l, m

AU

3,200

2,800

2,400

2,000

1,600

1,200

800

400

0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Volume, ml

100

50

0

%B

Single- and Multi-Wavelength DetectorsWhether you are running simple protein separations or purifying complex biological samples, you need accurate detection and buffer conductivity monitoring.

The NGC Single- (UV) and Multi-Wavelength (UV/Vis) Detector Modules provide highly accurate detection of biomolecules. Both are combined with an integrated conductivity monitor (0.01 to 999 mS/cm).

The single-wavelength UV detector contains an LED UV light source, with filters for 255 and 280 nm that enable the monitoring of nucleic acids or proteins.

The multi-wavelength UV/Vis detector adds flexibility to your chromatography system by simultaneously monitoring four wavelengths between 190 and 800 nm in a single module and achieving greater sensitivity in the detection of proteins, peptides, nucleic acids, and chromophores.

SAMPLE MONITORING AND FRACTION COLLECTION

A cation exchange standard containing a-lactalbumin, ribonuclease A, cytochrome C, and lysozyme was run on a 1 ml ENrich S Column at pH 6.5. The NGC Multi-Wavelength Detector was used to simultaneously monitor peptide bonds at 215 nm (—), aromatic rings within protein structures at 280 nm (—), and chromophores at 420 nm (—). This single run provided confirmation of the presence or absence of these compounds before any post-run analysis was performed.

Single-wavelength detector and multi-wavelength detector with signal import module

■ Integrated conductivity monitor (0.01–999 mS/cm)

■ Interchangeable flow cells with 2 mm (preparative), 5 mm (standard analytical), and 10 mm (analytical) path lengths

■ Single-wavelength detector (255 or 280 nm)

■ Multi-wavelength detector (190–800 nm)

Catalog # Description7884008 NGC Single-Wavelength Detector Module12010343 NGC Multi-Wavelength II Detector Module

pH Valve ModuleThe ability to determine and monitor pH is essential for method development and for most purification applications.

The NGC pH Valve Module has an integrated flow cell and pH electrode for accurate inline pH monitoring. It has a bypass mode to take the probe offline without the need for any replumbing.

■ Accurate inline pH monitoring (pH 1–14)

■ Calibration port for in situ calibration

■ Integrated bypass valve

■ Reference and pH electrodes sealed in a single body to avoid damage from drying

■ Temperature-compensated pH determination

Catalog # Description7884011 NGC pH Valve Module

pH valve

Signal Import ModuleAdding external devices to a purification workflow provides the flexibility to increase automation and the amount of information collected. The NGC Signal Import Module enables analog-to-digital signal conversion and connection to third-party devices.

■ Enables automated, accurate, and reproducible small- and large-volume sample injection through third-party autosamplers

■ Allows you to gather more information about your protein of interest using an external detector

Catalog # Description7884016 NGC Signal Import Module

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FRACTION COLLECTION

NGC Fraction Collector

Researchers require a versatile and easy-to-use fraction collector for their purification needs. The following fraction collection options are compatible with all NGC Systems.

NGC Fraction CollectorThe NGC Fraction Collector provides automated collection options for analytical and preparative chromatography applications. As Bio-Rad’s premier fraction collector, the NGC Fraction Collector is capable of performing basic to complex fraction collection schemes and can be used at flow rates up to 200 ml/min. It supports numerous rack and tray combinations, from microplates and tubes to bottles and carboys.

■ Enables multi-user support with increased capacity to accommodate multiple racks for multivessel collection

■ Maintains integrity of fractions with Peltier cooling

■ Achieves production batch capacity by collecting up to 80 unlimited volume fractions

■ Supports flow rates up to 200 ml/min

■ Allows accurate vessel positioning down to millimeter precision using factory programmed firmware

■ Collect with 2 NGC Fraction Collectors or 1 NGC Fraction Collector and the BioFrac Fraction Collector

■ Gain added versatility when used in conjunction with up to two 12-port outlet valves

10 | Module Selection Guide

■ Collect up to 12 large-volume fractions with single valve

■ Collect up to 23 large-volume fractions with two valves

■ Collect variable fraction sizes with the BioFrac Fraction Collector

■ Connect up to 2 fraction collectors to the NGC System

■ Redirect samples for Multi-D methods

Catalog # Description17002070 NGC Fraction Collector with Racks7410002 BioFrac Fraction Collector7884013 NGC Outlet Valve Module

Outlet valve

BioFrac Fraction Collector

BioFrac Fraction CollectorThe BioFrac Fraction Collector is ideal for both small- and large-scale preparative chromatography applications.

It offers flexible collection rack options, including 4 x 96-well plates, 0.5–2.0 ml microtubes, larger-volume tubes ranging in diameter from 12 to 20 mm, and preparative racks for collection in bottles.

■ Ideal for small- and large-scale preparations

■ Versatile sample collection capability from 96-well plate to 20 mm diameter tubes

■ Stand-alone operation

■ Added versatility when used in conjunction with up to two 12-port outlet valves

Outlet Valve

FRACTION COLLECTION

© 2020 Bio-Rad Laboratories, Inc. | 11

Inlets, blending valve, and system pumps

Sample loading Column switching and reverse flow

Sample monitoring Fraction collection

Water

Salt

Acid

Base

CONFIGURE YOUR NGC SYSTEM

10 ml/min pumps

100 ml/min pumps

Single-wavelength detector

Buffer blending valve module

Multi-Wavelength Detector II

Sample pump module

pH valve module

Signal import module

BioFrac Fraction CollectorAutosampler

NGC Fraction Collector

Module Selection Table

Inlet valve module (buffer)

Quantity

Inlet valve module (sample)

Quantity

Column switching valve module Quantity

Outlet valve module

Quantity

x 5

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