Methods in Enzymology

Volume 307

Con focal Microscopy

P. Michael Conn

CONTRIBUTORS TO VOLUME 307 ix

PREFACE xii i

VOLUMES IN SERIES xv

Section I. Theory and Practical Consideration s

1. Theoretical Basis of Confocal Microscopy

ROBERT H . WEBB

3

2. Practical Considerations in Acquiring Biological S . K. KONG ,

Signals from Confocal Microscope

SAMUEL Ko,C . Y . LEE, ANDP . Y . Lui

20

Section II . General Technique s

3. Equipment for Mass Storage and Processing of Data Guy Cox

2 9

4 . Antifading Agents for Confocal Fluorescence Mi- MIGUEL BERRIOS,croscopy

KIMBERLY A . CONLON, AN DDAVID E. COLFLESH

5 5

5. Mounting Techniques for Confocal Microscopy

MANABU KAGAYAMA AND

YASUYUKI SASANO

7 9

6 . Preparation of Whole Mounts and Thick Sections HISASHI HASHIMOTO ,for Confocal Microscopy

HIROSHI ISIIIKAWA, ANDMORIAKI KUSAKABE

84

7 . Cytotomography

TORSTEN PORWOL,

ANJA-ROSE STROHMAIER ,

AND EBERHARD SPIESS

10 8

8 . Use of Confocal Microscopy to Investigate Cell GIIASSAN BKAILY ,Structure and Function

DANIELLE JACQUES, ANDPIERRE POTHIER

11 9

9 . Combining Laser Scanning Confocal Microscopy XuEJUN SuN

13 5and Electron Microscopy

10. Construction of Line-Scan Confocal Microscope for NICK CALLAMARAS ANDPhysiological Recording

IAN PARKER

152

Section III . Measurement of Subcellular Relations andVolume Determination s

11 . Three-Dimensional Visualization of Cytoskeleton WERNER BASCHONG ,

by Confocal Laser Scanning Microscopy

MARKUS DORRENBERGER ,

ANNA MANDINOVA, AND

ROSMARIE SUET IERLIN

173

12 . Chromosome Spread for Confocal Microscopy

NADIR M. MARALDI ,

SILVANO CAPITANI ,

CATERINA CINTI,

LUCA M . NERI ,

SPARTACO SANTI ,

STEFANO SQUARZONI ,

LIBORIO STUPPIA, AND

FRANCISCO A . MANZOLI

190

13 . Visualization of Nuclear Pore Complex and Its Dis- ULRICH KUBITSCHECK ,

tribution by Confocal Laser Scanning Microscopy THORSTEN KUES, AND

REINER PETERS

207

14 . Measurement of Tissue Thickness Using Confocal JAMES V. JESTER,

Microscopy

W . MATTHEW PETROLL, AN D

H. DWIGHT CAVANAGH

23 0

15 . Measurements of Vascular Remodeling by Confo- SILVIA M . ARRIBAS ,

cal Microscopy

CRAIG J . DALY, AN D

JOHN C. MCGRATH

246

16 . Cell Volume Measurements by Fluorescence Con- LOTHAR A . BLATFER

27 4

focal Microscopy : Theoretical and Practical As-

pects

17 . Volume Measurements in Confocal Microscopy

GARY C . SIECK ,

CARLOS B . MANTILLA, AND

Y . S . PRAKASH

29 6

Section IV. Imaging of Processe s

18 . Quantitation of Phagocytosis by Confocal Mi- GEORGE F. BABCOCK

31 9

croscop y

19. Measurement of Secretion in Confocal Microscopy AKJHISA SEGAWA

328

20. Receptor-Ligand Internalization

GUIDO ORLANDINI ,

NICOLETTA RONDA,

RITA GATTI ,

GIAN CARLO GAZZOLA, AND

ALBERICO BORGHETTI

34 0

21 . Quantitative Imaging of Metabolism by Two- DAVID W . PISTON AND

Photon Excitation Microscopy

SUSAN M. KNOBEL

351

22 . Localization of Proteases and Peptide Receptors by CELIA J. SNYMAN,

Confocal Microscopy

DESHANDRA M . RAIDOO, AN D

KANTI D . BHOOLA

368

23 . Observation of Microcirculatory Kinetics by Real- KAZUHIRO YAMAGUCHI

394

Time Confocal Laser Scanning Microscop y

Section V. Imaging of Ions

24. Confocal Imaging Analysis of Intracellular Ions in HISAYUKI OHATA ,

Mixed Cellular Systems or in Situ Using Two MASAYUKI YAMAMOTO,

Types of Confocal Microscopic Systems

YOSUKE UJIKE ,

GOUSEI RIE, AND

KAZUTAKA MOMOSE

425

25 . Confocal Cat+ Imaging of Organelles, Cells, Tissues, DAVID ALAN WILLIAMS ,

and Organs

DAVID N. BOWSER, AND

STEVEN PETROU

441

26 . Using Confocal Microscopy and Fluorescent Indica- JON R . INGLEFIELD AND

tor, 6-Methoxy-N-ethylquinolinium Iodide, to ROCHELLE D .

469

Measure Changes in Intracellular Chloride

SCHWARTZ-BLOOM

Section VI. Imaging of Specialized Tissues

27 . Measurement of Mineral Gain and Loss on Dental CARLOS GONZALEZ-CABEZAS ,

Enamel and Dentin Using Confocal Microscopy MARGHERITA FONTANA, AND

GEORGE K. STOOKEY

482

28. Characterization of Ocular Cellular and Extracellu- DANIEL BROTCHIE ,

lar Structures Using Confocal Microscopy and NEIL ROBERTS ,

Computerized Three-Dimensional Recon- MIKE BIRCH,

struction

PENNY HOGG ,

C . VYVYAN HOWARD, AN D

IAN GRIERSON

496

29 . Multiphoton Excitation Microscopy, Confocal Mi- BARRY R . MASTERS ,

croscopy, and Spectroscopy of Living Cells and PETER T . C. So,

Tissues : Functional Metabolic Imaging of Human KI HEAN KIM,

Skin in Vivo

CHRISTOF BUEHLER, AND

ENRICO GRATTON

51 3

30 . Video-Rate, Scanning Slit Confocal Microscopy of BARRY R . MASTERS AND

Living Human Cornea in Vivo: Three-Dimen- MATTHIAS BÖHNKE

53 6

sional Confocal Microscopy of the Ey e

31 . In Vivo Imaging of Mammalian CNS Neurons RALF ENGELMANN AND

with ICON

BERNHARD A . SABEL

56 3

Section VII. Imaging Viruses and Fungi

32 . Identification of Viral Infection by Confocal Mi- DAVID N . HowELL AND

croscopy

SARA E . MILLER

573

33 . Using Confocal Microscopy to Study Virus Binding ALAIN VANDERPJ .ASSCHEN AND

and Entry into Cells

GEOFFREY L. SMITH

59 1

34. Fluorescent Labels, Confocal Microscopy, and RUSSELL N. SPEAR ,

Quantitative Image Analysis in Study of Fungal DANIEL CULLEN, AND

Biology

JOHN H . ANDREWS

607

AUTHOR INDEX 62 5

SUBJECT INDEX 647