New Diseases of P. Monodon and L. Vannamei [Compatibility Mode]
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Transcript of New Diseases of P. Monodon and L. Vannamei [Compatibility Mode]
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8/2/2019 New Diseases of P. Monodon and L. Vannamei [Compatibility Mode]
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Monodon Slow-Growth Syndrome
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Growth Retardation Phenomenon in culturedP. monodon
Culture Period (d) 118 13
Water Salinity (ppt) 11 8
Average Body Weight (g) 12.5 3.4
Average Daily Growth (g/d) 0.11 0.03
Coefficient of Variation of Body Weights (%) 66 39
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% of normal
size
shrimp
% of small
size
shrimpSingle infections 58 52MBV 38 30Microsporidian 13 6Gregarine 1 5HPV 0 2Bacteria 6 9
Dual infections 15 26MBV/HPV 7 10MBV/Microsporidian 7 6MBV/Gregarine 1 6HPV/Microsporidian 0 2Microsporidian/Gregarine 0 2Triple infections
9 7MBV/HPV/Microsporidian 7 5MBV/HPV/Gregarine 1 1MBV /Microsporidian/Gregarine 1 1
Quadruple infections 1 1Any of the tested pathogens 83 86None of the tested pathogens 17 15
Dual to quadruple infections 31 39Overall prevalence for each pathogen
MBV 63 60Microsproidians 29 23HPV 16 21Gregarines 5 16
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Criteria for the GrowthRetardation Syndrome
Growth retardation, ADG < .at mo Size difference, CV >
Abnormal appearance
No significant MBV or HPV infections
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Re-Infection Experiment Lymphoid organs of growth-retardedP. monodon from a commercial
farm
Lymphoid organ extract (LOE)
Homogenize (:with LHB)
Filtering through .mm
Stock healthyP. monodon ( - g BW, DOC< ) in l seawater
Tanks - = Control ( . ml LHB-injected)
Tanks - = . ml LOE injected
Determine BW, SR and general shrimp morphology at , and months
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Decreasing Size of the LOE-Injected Shrimp
Buffer-injected
LOE-injected
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Intense black-yellow stripes of LOE-injected shrimp
Buffer-injected
LOE-injected
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Buffer-Injected
CV (%)
BW (g)
LOE-Injected
CV (%)
BW (g)
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%SR
%CV
Buffer-injected LOE-injected
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MSGV = YHV Type
MSGV = nm virus = Laemsingh Virus
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)LHOG5HVXOWV VKRZHGWKDWRIWKHVORZJURZWKVKULPS
ZHUHYHZLWK573&5IRU
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-nm new
virus found in
both normal and
small shrimp Two types of
new viruses
LaemsinghVirus
Tegumental
gland-associatevirus
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Proportion and Growth Rateof Four Shrimp Families in a Common Pond
that Suffered from the Slow-Growth Phenomenon
.g/d.g/d .g/d.g/d
At Stocking At DOC At DOC
.g/d .g/d
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MSGA
Grow slowly but
survive better
(survive but the
price the shrimp
have to pay is
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Grow normally but
at low survival rate
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Positive Results of RT PCR for Laemsingh Virus from the Field
Small-sized
shrimp
Normal-sized
shrimp
P. monodon From slow-growth pond(No. of ponds =
From normal
L. vannamei
.
of ponds =
M. rosenbergii From slow-growth pond(No. of ponds =
Later on, with more data, the
virus was found in mostP.
monodon, small or normal size
Laemsingh virus and tegumental gland-
associate virus are candidates for being
MSGS
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Bamboo-ShapedL. vannamei