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New Blindingly fast – electrophoresis in 10 min! Bullet PAGE One … · 2020. 2. 17. · Bullet...
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Bullet PAGE One Precast Gel Features ► Only 10 minutes with 400 V ► High transfer efficiency of proteins on western blot membrane ► Works well in conventional Laemmli running buffer and sample buffer ► 17-well gel is usable with multichannel pipet for consistent loading Performance Comparison Bullet PAGE One gel can produce an excellent separation image with the shortest electrophoresis. Product Name Bullet PAGE One Company A Company B Gel ％ 5 - 15％ 4 - 15％ 4 - 12％ Product No. 13079-84 － － Electrophoresis Time 11 min. 33 min. 50 min. Electrophoresis Images M.W.(KDa) 200→ 45→ 6.5→ 22→ M.W.(KDa) 200→ 45→ 6.5→ 22→ M.W.(KDa) 200→ 45→ 6.5→ 22→ <Electrophoresis condition> Sample: Protein Markers(10x)(Prod No. 29458-24), 5 μl Gel Staining: CBB Stain One (Prod No. 04543) Voltage Constant: Bullet PAGE One: 400V, Company A and Company B: 200V Comparison of Protein Transfer Efficiency Bullet PAGE One gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min. Transfer 10V, 10 min. Transfer 10V, 20 min. Bullet PAGE One （5 - 15％ Gel） Bullet PAGE One （5 - 15％ Gel） Company A （4 - 15％ Gel） M.W.(KDa) 200→ 45→ 22→ M.W.(KDa) ←200 ←45 ←22 Company A （4 - 15％ Gel） ←6.5 ① ② ③ ④ ④ ③ ② ① 6.5→ M.W.(KDa) 200→ 45→ 22→ 6.5→ M.W.(KDa) ←200 ←45 ←22 ←6.5 ① ② ③ ④ ④ ③ ② ① High transfer efficiency High transfer efficiency <Sample> ① Protein Ladder One(Triple-color)(Product No. ： 09547-74) ② Protein Markers(3 × ) 2 μl added ③ Protein Markers(1 × ) 2 μl added ④ Protein Markers(1/3) 2 μl added <Transfer> Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31) (Transfer time : 10 min. and 20 min. 10V) <PVDF membrane staining> Stain PVDF membrane with CBB Stain One (Product No. ： 04543). Blindingly fast – electrophoresis in 10 min!
Transcript of New Blindingly fast – electrophoresis in 10 min! Bullet PAGE One … · 2020. 2. 17. · Bullet...
Bullet PAGE One Precast GelFeatures
► Only 10 minutes with 400 V ► High transfer efficiency of proteins on western blot membrane ► Works well in conventional Laemmli running buffer and sample buffer ► 17-well gel is usable with multichannel pipet for consistent loading
Performance Comparison
Bullet PAGE One gel can produce an excellent separation image with the shortest electrophoresis.Product Name Bullet PAGE One Company A Company B
Gel ％ 5 - 15％ 4 - 15％ 4 - 12％
Product No. 13079-84 － －
Electrophoresis Time 11 min. 33 min. 50 min.
Electrophoresis Images
M.W.(KDa)
200→
45→
6.5→
22→
M.W.(KDa)
200→
45→
6.5→
22→
M.W.(KDa)
200→
45→
6.5→ 22→
<Electrophoresis condition> Sample: Protein Markers(10x)(Prod No. 29458-24), 5 μl Gel Staining: CBB Stain One (Prod No. 04543) Voltage Constant: Bullet PAGE One: 400V, Company A and Company B: 200V
Comparison of Protein Transfer Efficiency
Bullet PAGE One gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min.
Transfer 10V, 10 min. Transfer 10V, 20 min.Bullet PAGE One（5 - 15％ Gel）
Bullet PAGE One（5 - 15％ Gel）
Company A（4 - 15％ Gel）
M.W.(KDa)
200→
45→
22→
M.W.(KDa)
←200
←45
←22
Company A（4 - 15％ Gel）
←6.5
① ② ③ ④④ ③ ② ①
6.5→
M.W.(KDa)
200→
45→
22→ 6.5→
M.W.(KDa)
←200
←45
←22
←6.5
① ② ③ ④④ ③ ② ①
High transfer efficiency
High transfer efficiency
<Sample> ① Protein Ladder One(Triple-color)(Product No. ： 09547-74) ② Protein Markers(3 × ) 2 μl added③ Protein Markers(1 × ) 2 μl added④ Protein Markers(1/3) 2 μl added
<Transfer> Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)(Transfer time : 10 min. and 20 min. 10V)<PVDF membrane staining> Stain PVDF membrane with CBB Stain One (Product No. ： 04543).
Blindingly fast – electrophoresis in 10 min!
1505
For research use only, not intended for diagnostic or drug use.
NACALAI TESQUE, INC.Nijo Karasuma, Nakagyo-ku, Kyoto 604-0855 JAPANTEL : +81-(0)75-251-1730FAX : +81-(0)75-251-1763Website : www.nacalai.comE-mail : [email protected]
Gel TypesGel Gradient Gel Single Percentage GelGel % 5 - 11% 5 - 15% 6% 8%
Product No. 13 well 17 well
13077-0413078-94
13079-8413080-44
13081-3413082-24
13083-1413084-04
Electrophoresis Images
M.W.(KDa)
200→
45→
22→
M.W.(KDa)
200→
45→
6.5→
22→
M.W.(KDa)
200→
45→
M.W.(KDa)
200→
45→
22→
Product SpecificationGlass Plate Size: W100mm × H80mm × T3.2mmGel Size: W80mm × H60mm × T1.0mmSample Well Configuration / Recommended Load Volume: 13-well / < 20 μl, 17-well / < 14 µl
VideosElectrophoresis movie of Bullet PAGE One gel is accessible by visiting link below (YouTube). http://www.nacalai.co.jp/information/movie/bullet.html
Related Products
WEP-N Vertical Electrophoresis TankProduct Name: WEP-N Vertical Electrophoresis TankProduct No.: WEP-NSize: 154W × 88D × 146H (mm)Required Buffer Volume: 800 ml
Ordering Information
Product Name Well Grade Storage Product No. PKG SizeBullet PAGE One Precast Gel, 5-11% 13 SP R 13077-04 10 Sheets
Bullet PAGE One Precast Gel, 5-11% 17 SP R 13078-94 10 Sheets
Bullet PAGE One Precast Gel, 5-15% 13 SP R 13079-84 10 Sheets
Bullet PAGE One Precast Gel, 5-15% 17 SP R 13080-44 10 Sheets
Bullet PAGE One Precast Gel, 6% 13 SP R 13081-34 10 Sheets
Bullet PAGE One Precast Gel, 6% 17 SP R 13082-24 10 Sheets
Bullet PAGE One Precast Gel, 8% 13 SP R 13083-14 10 Sheets
Bullet PAGE One Precast Gel, 8% 17 SP R 13084-04 10 Sheets (Storage) R : Refrigerate
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