Neural Representations of Airflow in Drosophila Mushroom Body
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Transcript of Neural Representations of Airflow in Drosophila Mushroom Body
Neural Representations of Airflow in Drosophila Mushroom BodyAkira Mamiya1, Jennifer Beshel1, Chunsu Xu1,2, Yi Zhong1*1 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States of America, 2 SUNY Stony Brook, Stony Brook, New York, United States of America
Take home points:
Goal: characterize the responses of MB neurons to changes in airflowMethod: In vivo calcium imaging from multiple MB regions using genetically altered fruit fly lines and 2-photon microscopyResults:
•Responses to an airflow stimulus from several sub regions of the MB•Different MB sub regions responded differently to different aspects (i.e. on/off responses)•Possibly sub sub regions respond differently•Dependent on the movement of the 3rd antennal segment suggesting JO involvement
Method1) Get TG fruitflies that have a calcium sensor
• UAS-G-CaMP1.3 • UAS-G-CaMP1.6
2) Cross with GAL4 fruit fly lines that will allow targeted expression of the calcium sensor in specific cells• OK107-Gal4: non-selective MB general• c739-Gal4: A/B lobe neurons• g0050-Gal4 and c305a-Gal4: A’/B’ lobe neurons
3) Fix fruit fly to an imaging stage4) Puff air and measure fluorescence:
• 3s stims • 100 ml/min (1.2m/s )• 3 min ITI
5) Analyses based on ΔF/Fo which is a measure of changes in Ca++ induced florescence on a pixel by pixel basis
Experimental recording sites and raw ΔF/Fo Figure 1
Temporal dynamics of responses 1 s data integration window (3 frames)Averaged results
Figure 2
Figure 2Mean On, off responses as a function of lobes
The total area involved in response
Figure 3
•OK107-Gal4: non-selective MB general•c739-Gal4: A/B lobe neurons•g0050-Gal4 and c305a-Gal4: A’/B’ lobe neurons
Different Gal4 lines show that A,B and A’B’ have different response profiles
Figure 4Responses generally depend on movement of the 3rd antennal segment•When glued Ca++ responses are greatly diminished
Figure 4
By comparison with Figure 2 most areas have dropped
Responses generally depend on movement of the 3rd antennal segment•When glued Ca++ responses are greatly diminished
Figure 5Sub region specific responses to “on” and “off” phases of the stimulus
Figure 5Watershed Segmentation highlights sub region specific responses
Figure 6“On Off Selectivity Index” (OSI) highlights watershed “patches” within lobes as “on” or “off” response selective (or neither)
Figure 7Off responding patches are spatially organized and stereotypic across individuals
Figure 8Using two Gal4 lines they show that the off and on responses are different subsets of cells
Figure 8
g0050-Gal4 cells are significantly more off responsive than c305a-Gal4