R&D Activities National Botanical National Botanical Research Institute, Research Institute,

LucknowLucknow (Herbal Technology)(Herbal Technology)

R&D Activities National Botanical National Botanical Research Institute, Research Institute,

LucknowLucknow (Herbal Technology)(Herbal Technology)

Dr. P. PushpangadanDirector

National Botanical Research Institute, Lucknow

National Botanical Research Institute, National Botanical Research Institute, LucknowLucknowA premier plant research center of India under the umbrella of CSIR by undertaking both basic and applied research in various aspects of plant sciences for the conservation and sustainable utilization of plant genetic resources for human welfare and sustainable.

(Dr. P. Pushpangadan)

Management Council Research Council

Economic Botany Information Service

Information Liaison and Common Facilities

Planning and Coordination

Experimental Research stations

House KeepingAuxilliary

Natural Product Development

Genetics & Plant Breeding and Agrotechnology

Biomass Biology and Environmental Sciences

Molecular Biology and Genetic Engineering

Biotechnology and Plant Physiology

Botanic garden and Floriculture

Plant Biodiveristy and Conservation Biology

Microbiology &

Plant Pathology

MissionMissionThe mission of the National The mission of the National Botanical Research Institute is:Botanical Research Institute is:

To act as a premier plant research To act as a premier plant research centre of India under the umbrella of centre of India under the umbrella of CSIR by undertaking both basic and CSIR by undertaking both basic and applied research in various aspects of applied research in various aspects of plant sciences for the conservation plant sciences for the conservation and sustainable utilization of plant and sustainable utilization of plant genetic resources for human welfare genetic resources for human welfare and sustainable development.and sustainable development.

VisionVision To be a premier national and international centre To be a premier national and international centre

on plant science research and development in the on plant science research and development in the areas of plant biodiversity, conservation & areas of plant biodiversity, conservation & sustainable utilization of non-crop and wild Plant sustainable utilization of non-crop and wild Plant Genetic Resources (PGR), bioprospecting, Genetic Resources (PGR), bioprospecting, biofuels, bioinformatics and plant biotechnology.biofuels, bioinformatics and plant biotechnology.

To be a prime catalyst in conversion of plant To be a prime catalyst in conversion of plant wealth into economic wealth in an ecologically wealth into economic wealth in an ecologically sound and economically rewarding manner.sound and economically rewarding manner.

To develop as a national referral centre for all To develop as a national referral centre for all knowledgebase related to plant biodiversity.knowledgebase related to plant biodiversity.

MandateMandate

To undertake both basic and applied To undertake both basic and applied research on plant sciences in taxonomy research on plant sciences in taxonomy (including biosystematics and molecular (including biosystematics and molecular taxonomy), conservation biology, taxonomy), conservation biology, pharmacognosy, ethnopharmacology, pharmacognosy, ethnopharmacology, biomass biology, environmental sciences, biomass biology, environmental sciences, microbiology, plant biotechnology (tissue microbiology, plant biotechnology (tissue culture and molecular biology including culture and molecular biology including genetic engineering), plant physiology, genetic engineering), plant physiology, Phytochemistry, genetics and plant Phytochemistry, genetics and plant breeding, floriculture and bioinformatics of breeding, floriculture and bioinformatics of the non-crop and wild plant genetic the non-crop and wild plant genetic resources of India.resources of India.

Biodiversity research by inventorying, monitoring, assessment, conservation and sustainable utilization of plant genetic resources of India with special focus on medicinal, aromatic, dye Bioprospecting for search of commercially valuable genes, biodynamic compounds and development of scientifically validated, value-added and standardized noval plant products-herbal drugs, pharmaceuticals, nutraceuticals and cosmaceuticals. Biomass biology including biopesticides, biofuels and petroleum alternatives and environmental sciences including phytoremediation and abatment of terrestrial and aquatic pollution including studies on greenhouse gas emission.

MAJOR R & D PROGRAMMES

Biotechnology (biotech process/products) including trans-disciplinary studies on molecular biology and genetic engineering for development of transgenic plants.

Genetics, plant breeding and agrotechnology Selection and genetic enhancement for development of new promising varieties of economically important non-crop plants.

Bioinformatics by developing computerized/ electronic databases on Indian plants with special focus on medicinal, aromatic, dyes, gum and tannin yielding plants and products and applications of IT in plant science research.

MAJOR R & D PROGRAMMES

Herbal products Herbal products developed developed

by NBRIby NBRI Herbal fermented drink Herbal fermented drink Herbal Soft DrinkHerbal Soft Drink Herbal lipstick (Luvstik)Herbal lipstick (Luvstik) Herbal GulalHerbal Gulal Nutraceuticals productsNutraceuticals products Neem productsNeem products Organic cultivation using microbial Organic cultivation using microbial

technology/ vermiculture etc.technology/ vermiculture etc.

Products in pipelineProducts in pipeline

Wound healing ointmentWound healing ointment Anti-ulcer formulationAnti-ulcer formulation Anti-diarrhoreal syrupAnti-diarrhoreal syrup Anti-oxidant formulationAnti-oxidant formulation

Aims and objectives of Aims and objectives of Pharmacognosy & Ethnopharmacology Pharmacognosy & Ethnopharmacology

DivisionDivision To carry out detailed pharmacognostic studies which To carry out detailed pharmacognostic studies which

includes correct taxonomic identification of plants and includes correct taxonomic identification of plants and it parts, macro- and microscopical details and it parts, macro- and microscopical details and histochemical analysis of the plant parts used as histochemical analysis of the plant parts used as medicine. The SEM studies are also under taken for the medicine. The SEM studies are also under taken for the identification/authentication of those commercial identification/authentication of those commercial samples, which are available in broken or small pieces. samples, which are available in broken or small pieces.

To develop chemical markers for the identification, To develop chemical markers for the identification, quality and batch-to-batch consistency of herbal drugs quality and batch-to-batch consistency of herbal drugs with the help of HPTLC, HPLC, GLC & DNA finger with the help of HPTLC, HPLC, GLC & DNA finger printings etc.printings etc.

Contd..

To develop physico-chemical standards of crude as well as To develop physico-chemical standards of crude as well as finished herbal drugs/products. finished herbal drugs/products.

To detect the microbial contamination including the detection of To detect the microbial contamination including the detection of mycotoxins in herbal drugs (raw as well as finished) in mycotoxins in herbal drugs (raw as well as finished) in accordance with the latest WHO guidelines. accordance with the latest WHO guidelines.

To estimate concentration of heavy metals and pesticide To estimate concentration of heavy metals and pesticide residue in herbal drugs as per WHO guidelines. residue in herbal drugs as per WHO guidelines.

A small animal house and a pharmacology lab has also been A small animal house and a pharmacology lab has also been established to cover the safety aspects or toxicity and other established to cover the safety aspects or toxicity and other pharmacological evaluation of herbal products. pharmacological evaluation of herbal products.

Besides, the survey work for collection of authentic samples i. e. Besides, the survey work for collection of authentic samples i. e. resource survey, market surveillance, shelf-life studies, seasonal resource survey, market surveillance, shelf-life studies, seasonal and ecological variations, effect of ‘Shodhan Sanskar’ and and ecological variations, effect of ‘Shodhan Sanskar’ and scientific validation of ethnobotanical claims are some of the scientific validation of ethnobotanical claims are some of the important activities of the group.important activities of the group.

The Current The Current InnovationInnovation

Herbal slimming/ anti Herbal slimming/ anti obesity agentobesity agent

Based on certain traditional leads we have Based on certain traditional leads we have screened a large number of medicinal screened a large number of medicinal plants and identified the potential ones. plants and identified the potential ones. Further screening narrowed down to the Further screening narrowed down to the most promising ones. most promising ones.

These were then subjected to detailed These were then subjected to detailed chemical and pharmacological chemical and pharmacological standardization including the various standardization including the various ecotypes of the species selected.ecotypes of the species selected.

Contd…

Activity guided extraction(s) and their Activity guided extraction(s) and their various combination their of were made various combination their of were made and evaluated pharmacologically.and evaluated pharmacologically.

Various combinations were made with the Various combinations were made with the help of the best Ayurveda experts.help of the best Ayurveda experts.

The final formulation (including The final formulation (including standardized extracts) was made by a standardized extracts) was made by a team of scientists drawn from Ayurveda, team of scientists drawn from Ayurveda, Pharmacognosy, Medicinal chemistry, Pharmacognosy, Medicinal chemistry, Pharmacology and Pharmacy.Pharmacology and Pharmacy.

FormulationFormulation Contain 4 medicinal plants.Contain 4 medicinal plants. The extracts of the selected The extracts of the selected

plants were lyophilized and plants were lyophilized and used in the preparation of used in the preparation of the final formulation.the final formulation.

Chemical evaluationChemical evaluation Some chemical moieties/marker Some chemical moieties/marker

compounds present in the chosen plants compounds present in the chosen plants were identified.were identified.

Chemo/ fingerprint profiling of the Chemo/ fingerprint profiling of the extracts were done using modern extracts were done using modern sophisticated techniques like HPTLC/ sophisticated techniques like HPTLC/ HPLC.HPLC.

Profiling of the final product was also Profiling of the final product was also recorded.recorded.

Pharmacological Pharmacological evaluationevaluation

S D Rats (over weight) were used S D Rats (over weight) were used in experimentationin experimentation

Monitored:Monitored: Body weight profileBody weight profile

Biochemical estimationsBiochemical estimations TriglyceridesTriglycerides CholestrolCholestrol PhosphilipidsPhosphilipids

Pharmacological findingsPharmacological findings Over weight was significantly Over weight was significantly

reverted back.reverted back. Significant reduction inSignificant reduction in

PHOSPOLIPIDS and CHOLESTROL PHOSPOLIPIDS and CHOLESTROL TRIGLYCERIDE levelTRIGLYCERIDE level was maintained was maintained in the treated groups.in the treated groups.

Toxicological Toxicological evaluationevaluation

Given orally does not shown Given orally does not shown any gross abnormality or any gross abnormality or mortality in acute toxicity mortality in acute toxicity

studies studies

IPRIPRIt is protected by It is protected by

patenting in patenting in US and PCTUS and PCT..

ContactContact

Dr. P. PushpangadanDr. P. PushpangadanDirectorDirectorNational Botanical Research Institute,National Botanical Research Institute,Rana Pratap Marg, Post Box 436Rana Pratap Marg, Post Box 436Lucknow-226 001 (U.P.) IndiaLucknow-226 001 (U.P.) IndiaPh.: 0522-2205848, 2205839Ph.: 0522-2205848, 2205839Fax: 0522-2205836, 2205839Fax: 0522-2205836, 2205839email: email: pushpangadan@satyam.net.inpushpangadan@satyam.net.in