Nassir Precentaion

8/14/2019 Nassir Precentaion

1/16

AA Comparative Study BetweenComparative Study BetweenProstatic Acid Phosphatase EnzymeProstatic Acid Phosphatase EnzymeAnd Prostatic -Specific Antigen InAnd Prostatic -Specific Antigen In

Diagnosis And Monitoring Of ProstateDiagnosis And Monitoring Of Prostate

CancerCancerBy/ Nassir hassan Mohammed elssayedBy/ Nassir hassan Mohammed elssayed

)Batch )25)Batch )25Department of chemical pathologyDepartment of chemical pathology

:Supervisor:SupervisorMr. Abo bakr HassanMr. Abo bakr Hassan

Faculty of Medical Laboratory ScienceFaculty of Medical Laboratory Science

University of GeziraUniversity of GeziraSe tember 2006


2/16

IntroductionIntroduction

prostate is male's gland. it starts to developprostate is male's gland. it starts to developat twelfth week of fetal life under theat twelfth week of fetal life under theinfluence of androgenic hormones from theinfluence of androgenic hormones from thefetal testis.fetal testis.

The normal size of prostate is 20g but can beThe normal size of prostate is 20g but can beincreased variably with malignancy but mayincreased variably with malignancy but maybe 100- 200% larger in benign prostaticbe 100- 200% larger in benign prostatichyperplasia )BPH.hyperplasia )BPH.

Prostate gland has a sexual function, and itProstate gland has a sexual function, and itforming secretion that may protect the lowerforming secretion that may protect the lowerurinary tract and reproductive system fromurinary tract and reproductive system from

the insult of pathogens.the insult of pathogens.


3/16

-: Enlargement of prostate-: Enlargement of prostate

Benign prostatic hyperplasia )BPH.Benign prostatic hyperplasia )BPH. Prostate cancer.Prostate cancer. Diagnosis of prostate enlargement :-Diagnosis of prostate enlargement :- prostatic acid phosphates )PAPprostatic acid phosphates )PAP

prostate specific antigen )PSAprostate specific antigen )PSA Biology of PSA .Biology of PSA . Protein Binding PSA.Protein Binding PSA. PSA clearance.PSA clearance.

Normal value:Normal value: PAP : 1.7 U/LPAP : 1.7 U/L PSA : Up to 4 ng/ml .PSA : Up to 4 ng/ml . -clinical important :-clinical important :


4/16

Justification and objectivesJustification and objectives

JustificationJustification 1. Prostate cancer is a serious disease and if is1. Prostate cancer is a serious disease and if isnot treated it can lead to death.not treated it can lead to death.

2. There is difficult in diagnosis and monitoring of2. There is difficult in diagnosis and monitoring ofprostate cancerprostate cancer

ObjectiveObjective 1. General objective1. General objective To compare serum PSA level with that of serumTo compare serum PSA level with that of serum

acid phosphates level.acid phosphates level. 2. Specific objective2. Specific objective To measure serum PSA levelTo measure serum PSA level To measure serum acid phosphates levelTo measure serum acid phosphates level To determine which of the tow measurementTo determine which of the tow measurement

should be recommended in diagnosis andshould be recommended in diagnosis and

monitoring of prostate cancer.monitoring of prostate cancer.


5/16

Material & methodsMaterial & methods

Study area and duration .Study area and duration . Sample size .Sample size . Equipment .Equipment . Reagent .Reagent . Methods for laboratory investigationMethods for laboratory investigation Determination of serum prostatic acidDetermination of serum prostatic acid

phosphatesphosphates

Principle:Principle:Alpha-naftyle phosphate + HAlpha-naftyle phosphate + H22O alpha-O alpha-

naphtol + phosphatenaphtol + phosphate

Alpha-naphtol + fast Red TR AZO dyeAlpha-naphtol + fast Red TR AZO dye


6/16

Determination of serum total PSA.Determination of serum total PSA.a Added 200nl assay buffer to 20nla Added 200nl assay buffer to 20nl

stander/sample, vortex and added onestander/sample, vortex and added onebead to them. Then incubated 2 hours onbead to them. Then incubated 2 hours on

rotator.rotator.b Added 2ml wash buffer and decanted theb Added 2ml wash buffer and decanted the

solution )repeated twice.solution )repeated twice.

c Added 200nlc Added 200nl 125125I tracer solution. IncubatedI tracer solution. Incubatedon rotator about 20hours.on rotator about 20hours.

d Then added 2ml wash buffer andd Then added 2ml wash buffer anddecanted the solution )repeated twice.decanted the solution )repeated twice.


7/16


8/16

Result & discussionResult & discussionGroups = Experimental

Table (1) Shows mean and std deviation of P.S A and P A P

8.931696.64457PAP

190.2668191.5422Total PSA

Std DeviationMean

.000495Total PSA&PAP

Sig (2-tailed )Correlation


9/16

std Devituon PSA PAP

(Experimental)

8.93169

190.2668

0

50

100

150

200

pappsa

toal psa & pap

stdSeries1

mean PSA PAP (Experimental)

6.64457

191.5422

0

50

100

150

200

250

pappsa

toal psa & pap

mean

Series1

Fig (1)

SHOWS MEAN & STD Deviation of PSA & PAP


10/16

GROUPS : CONTROL

Table (2)

Shows mean and std/ deviation of total PSA and PAP

.495031.00286PAP

.96044.0714Total PSA

Std Deviation

Mean

.001-.837Total PSA&PAP

Sig (2-tailed )Correlation


11/16

Fig (2)

SHOWS MEAN & STD Deviation of PSA & PAP

mean PSA PAP (control)

1.00286

4.0714

0

1

2

3

4

5

pappsa

toal psa & pap

mean

Series1

std Devituon PSA PAP

(control)

0.49503

0.9604

0

0.2

0.4

0.6

0.8

1

1.2

pappsa

toal psa & pap

stdSeries1


12/16

DiscussionDiscussion

This study done on 30 sample attended This study done on 30 sample attendedinstitute of nuclear medicine molecularinstitute of nuclear medicine molecularBiology. 23 of them is experimental groupBiology. 23 of them is experimental group

and 7 of them is group control. The ageand 7 of them is group control. The agebetween 50-90 years.between 50-90 years.

The result obtained from the prostheticThe result obtained from the prostheticacid phosphates test and total PSA.acid phosphates test and total PSA.

The result shows in table )1 fig )1 The result shows in table )1 fig )1include the mean std deviation andinclude the mean std deviation andsignificant for sample tested groupsignificant for sample tested group

ex erimental for PAP test and total PSA.experimental for PAP test and total PSA.


13/16

mean of PAP)6.64457 the differencemean of PAP)6.64457 the differenceis significant, the level of significantis significant, the level of significantis ).000is ).000

The result shows table )2 fig)2 The result shows table )2 fig)2include the mean std deviation andinclude the mean std deviation andsignificant for sample tested groupsignificant for sample tested groupcontrol for PAP test and total PSA.control for PAP test and total PSA.

The mean of total PSA was )4.0714The mean of total PSA was )4.0714this high than mean of PAP )1.00286this high than mean of PAP )1.00286the difference is significant. The levelthe difference is significant. The level

of si nificant is ).001of significant is ).001


14/16

Conclusion &-5Conclusion &-5

:recommendation:recommendation

Conclusion:Conclusion: There is significant difference There is significant difference

between acid phosphates estimationbetween acid phosphates estimationcompared to PSA estimation level incompared to PSA estimation level indiagnosis of prostate cancer.diagnosis of prostate cancer.

The serum PSA is moor accurate The serum PSA is moor accurateand precise than serum PAP inand precise than serum PAP indiagnosis and monitoring of prostaticdiagnosis and monitoring of prostaticcarcinoma.carcinoma.


15/16

RecommendationRecommendation::

PSA is time consuming andPSA is time consuming andexpensive I recommend the test toexpensive I recommend the test to

be done in a shorter time and withbe done in a shorter time and withminimal expensive.minimal expensive.


16/16

Nassir Precentaion

Documents

Transcript of Nassir Precentaion