Name ____________________________________ Date ... · Web viewTranslation: prokaryotes vs....
Transcript of Name ____________________________________ Date ... · Web viewTranslation: prokaryotes vs....
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Name ____________________________________ Date ____________ Period _____
PROTEIN SYNTHESIS: TRANSLATIONRaven Ch. 15
Big Idea 3: Living systems store, retrieve, transmit, and respond to information essential to life processes.
Essential Knowledge: Heritable information provides for continuity of life. Expression of genetic information involves cellular and molecular mechanisms. The processing of genetic information is imperfect and is a source of genetic variation. Cells communicate by generating, transmitting and receiving chemical signals. Transmission of information results in changes within and between biological systems.
Translation-- from _________________ language to __________________ language
Cracking the code
Crick
determined _____________________________ system
WHYDIDTHEREDBATEATTHEFATRAT
Nirenberg (47) & Khorana (17)
determined mRNA–amino acid match
added fabricated mRNA to test tube of ribosomes, tRNA & amino acids
created artificial UUUUU… mRNA
found that UUU coded for phenylalanine
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Code for ALL life!
strongest support for a _____________________ for all life
Code is ___________________________
several codons for each amino acid
3rd base “____________________”
Start codon
___________________
___________________
_________________ codons
UGA, UAA, UAG
How are the codons matched to amino acids?
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Transfer RNA structure ( __________________)
“Clover leaf” structure
o _____________________ on “clover leaf” end
o ___________________ attached on 3 end
Loading tRNA
Aminoacyl tRNA synthetase
enzyme which bonds _____________ to _____________
bond requires energy
ATP AMP
bond is unstable
so it can release amino acid at ribosome easily
______________________
Facilitate coupling of tRNA _____________ to mRNA __________
organelle or enzyme?
Structure
ribosomal RNA (________) & __________________
2 subunits
large
small
____ site (aminoacyl-tRNA site)
holds tRNA carrying next amino acid to be added to chain
____ site (peptidyl-tRNA site)
holds tRNA carrying growing polypeptide chain
____ site (exit site)
empty tRNA leaves ribosome from exit site
Building a polypeptide
________________
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brings together mRNA, ribosome subunits, initiator tRNA
________________
adding amino acids based on codon sequence
________________
end codon
Protein targeting
Signal peptide
address label
Protein Synthesis in Prokaryotes
Prokaryote vs. Eukaryote genes
Prokaryotes Eukaroyotes
_________________ _________________
_________________ _________________
_________________ _________________
_________________ _________________
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Destinations:o secretiono nucleuso mitochondriao chloroplastso cell membraneo cytoplasmo etc…
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Translation in Prokaryotes
Transcription & translation are ___________________in bacteria
DNA is in _________________
no mRNA _________________
ribosomes read mRNA ______________________
Translation: prokaryotes vs. eukaryotes
Differences between prokaryotes & eukaryotes
__________________ separation between processes
takes eukaryote ~1 hour
from DNA to protein
no RNA processing
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