NEUROSPORA (MONILIA) HORMODENDRUM SCOPULARIOPSIS PAECILOMYCES SYNCEPHALASTRUM CEPHALOSPORIUMCOLONIAL MORPHOLOGY

White at first but grows rapidly filling the entire Petri dish in a few days and becoming a salmon to brown color. Mycelium may hang from the lid of the Petri dish.

Green to gray to black colony on both sides. Often wrinkled and grows flat

Powdery, light brown, wrinkled colony resembling Penicillium spp. Except for color.

Flat, rapid-growing, tan-colored colony resembling Aspergillus spp.

Gray to brown to black fluffy colony that may fill a Petri dish in a few days. Similar to Mucor and Rhizopus spp.

White to tan to rose-colroed colony, eventually developing White aerial hyphae.

CULTURE PHOTO

MICROSCOPIC MORPHOLOGY

Clear septate hyphae with large masses of ovate spores which are air-dispersed

Dark brown septate hyphae bear branching chains of elongate to ovate spores that often contain a small black dot at the end. Spore bearing structures look tree-like

Resembles Penicillium spp. except spores are larger and form unbranched-chains

Similar to Penicillium spp. except small spores are produced on very long, slender structures

Broad, clear, nonseptate hyphae, spores in many slender sac-like structures (sporangia) adhere to a swelling on the terminal end of hypha.

Single celled, clear, elliptical spores held together in a ball unless broken loose

MICROSCOPE PICTURE

LAB CULTURE

LAB MICROSCOPE

NIGROSPORA CHAETOMIUM BOTRYTIS STEMPHYLUM SEPEDONIUM GLIOCLADIUMCOLONIAL MORPHOLOGY

Rapid growing, producing abundant fluffly, aerial hyphae, gray to black on both sides. Resembles Mucor or Rhizopus

Slow growing, flat, white, yellow, tan or brown colony

Soft looking tan to gray colony Brown to black, wrinkled fuzzy colony

Fluffy, white colony resembles Histoplasma capsulatum

Flat, rapid-growing colony. White at first, then developing dark green central portion

CULTURE PHOTO

MICROSCOPIC MORPHOLOGY

Large, clearly visible jet black spores. Large, dark, central structure is perithecium that contains ascospores (sexually produced)

Colorless, one celled spores borne in clumps

Hyphae are brown and strongly septate: huge multicelled terminal spores that may either be smooth or rough

Large, rough-walled spores that resembles Histoplasma capsulatum

Numerous small spores held together in a clump. Similar to Penicillium spp. Except for the clumping of spores.

MICROSCOPE PICTURE

LAB CULTURE

LAB MICROSCOPE

ASPERGILLUM PENICILLIUM MUCOR RHIZOPUS FUSARIUM GEOTRICHIUMCOLONIAL MORPHOLOGY

Flat, compact colonies, white at first then becoming black, green, bluish or yellow

White colony at first but developing blue to green color

Cottony, rapid grower. May completely fill a Petri dish in 3 to 5 days. Brown to gray.

Gray to brown to black colony filling a Petri dish in 2 to 3 days. Similar to Mucor spp.

Fast-growing colony. At first, white and cottony but develping rose to red color on both sides

White to tan, flat or fluffy, rapid-growing fungus

CULTURE PHOTO

MICROSCOPIC MORPHOLOGY

Small one-celled spores irradiating out from swollen base (see arrows)

Small, round spores borne in “brush-like” formations

Clear, nonseptate hyphae. Spores borne inside large spherical structures called sporangia. Similar to Rhizopus spp. but lacking rhizoids.

Similar to Mucor spp. except foot-like structures (rhizoids) at base of spore bearing hyphae (see arrows). Spores in sporangium clear, coenocytic hyphae

Largest spores are sickle-shaped and may contain several cells. Small spores with one to two cells have more rounded ends.

Note hyphae breaking into arthrospores. May be confused with Coccidioides immitis.

MICROSCOPE PICTURE

LAB CULTURE

LAB MICROSCOPE

ALTERNARIA CURVALARIACOLONIAL MORPHOLOGY

Rapid-growing colonies, grayish to black to brown; underside jet black

Dark brown to black colony on both sides ragged in appearance.

CULTURE PHOTO

MICROSCOPIC MORPHOLOGY

Large, hand grenade-shaped spores with both longitudinal and transverse cross walls. Borne singly or in chains. Septate, dematiaceous fungi.

Large, bent spores with 3 to 5 cells. Similar to Helminthosporium spp. Brown, septate hyphae.

MICROSCOPE PICTURE

LAB CULTURE

LAB MICROSCOPE

TRICHODERMA SPECIESClassification: ContaminantsMounting fluid used: Lactophenol Cotton Blue (LPCB)

TRITRACHIUM SPECIESClassification: ContaminantsMounting fluid used: LPCB

DRESCHLERA SPECIESClassification: ContaminantsMounting fluid used: LPCB

MALASSEZIA FURFURKOH Smear – Skin scrapings Positive for Short hyphal elements with oval bodies(Malassezia furfur)

-COPYRIGHT © 2007-MICROBIOLOGY – MYCOLOGY LAB 1 – OPPORTUNISTIC MYCOSESUSTMED ’07 Sec C – AUDS M.; pictures provided by JV.NAVARRO

TEASE MOUNT TECHNIQUE

For the tease mount, a fragment of the colony collected using a wire or loop is transferred to a glass microscope slide. A drop of lactophenol cotton blue is then added, and the specimen is teased using dissecting needles as shown in this figure. The teasing of the specimen needs to be done carefully. Isolated elements can be observed, while at the same time preserving the integrity of the over-all structure of the microorganism

ADHESIVE TAPE TECHNIQUE

A piece of transparent adhesive tape is used to collect the specimen by pressing the adhesive slide against the surface of the fungal colony. Aerial elements will adhere to the tape, which is subsequently placed on a microscopic slide containing a drop of lactophenol cotton blue. This technique is good for preserving the original relationship between spores and aerial hyphae. However, it usually cannot be applied to mold specimens that

have few aerial mycelia or to yeast with a moist consistency.