1. 1. Membrane-targeted DNA nanostructures Folding of a long single strand of DNA (e.g. viral) aided by multiple smaller "staple" strands. Sanderson 2010 Rothemund et al 2006
2. 2. Six helix bundle (6HB) Alexa488 TEG-CH To consturct a rod-like DNA nanostructure (422nm x 6nm) with cholesteryl moieties (spacing of 42 nm=12 helical turns) on one side labeled with fluorofor on the other side Used to follow initial steps in protein-assisted membrane sculpting
3. 3. DOPC/BSM/Chol 2/2/1 - addition of MgCl2 (final conc. 10mM) CH-modified 6HB on phase separated vesicles switchable partitioning to different domains DiD - marker for disordered membrane domains Origami DNA (6HB) labeled with Alexa 488
4. 4. Translational and rotational diffusion
5. 5. Origami DNA monoliths